Aleksandra Bočarov-Stančić | University of Belgrade (original) (raw)

Papers by Aleksandra Bočarov-Stančić

Research paper thumbnail of Relationships of mycobiota on rachides and kernels of wheat

European Journal of Plant Pathology, 2012

The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14... more The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r00.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r00.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r0−0.3783 *) and kernels (r0−0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.

Research paper thumbnail of Toxigenic potential Fusarium langsethiae isolates from Serbian wheat kernels

Cereal Research Communications

Research paper thumbnail of Overview on toxigenic fungi and mycotoxins in Serbia and Montenegro

Research paper thumbnail of Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Pesticidi i fitomedicina, 2012

A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sun... more A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies.

Research paper thumbnail of The influence of mineral adsorbent „Minazel” on the reduction of residual zearalenone and its metabolites in cow's milk

Veterinarski glasnik, 2003

Izvod: U radu su prikazani rezultati kori{}enja adsorbenta mikotoksina -Minazel, preparata na baz... more Izvod: U radu su prikazani rezultati kori{}enja adsorbenta mikotoksina -Minazel, preparata na bazi prirodnog zeolita, u prevenciji mikotoksikoza i spre~avanju prelaska (akumuliranja) zearalenona i njegovih metabolita u mleku krava. Mikotoksikolo{kim analizama mleka utvr|eno je da je prisustvo zearalenona u mleku (0,053; 0,019 i 0,004 mg/kg) opadalo sa pove}anjem koli~ine Minazela u koncentratu (0; 0,2; 0,5), {to uz ostvareno pove}anje proizvodnje mleka opravdava njegovo kori{}enje.

Research paper thumbnail of An outbreak of Aspergillus species in response to environmental conditions in Serbia

Pesticidi i fitomedicina, 2013

The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wh... more The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.

Research paper thumbnail of Influence of Min-a-zel Plus on biochemical, microbiological and mycotoxicological parameters of lucerne silage

Journal of Agricultural Sciences, Belgrade, 2003

Lucerne of the 5 th cut in the budding phase was ensiled in the experiment as fresh and wilted (D... more Lucerne of the 5 th cut in the budding phase was ensiled in the experiment as fresh and wilted (DM= 220 and 360 g/kg), with two degrees of compression (520 and 380 g/dm 3 ) and with added Min-a-zel plus as 2 g/kg green mass. The experiment was performed as the statistical model 2 K .

Research paper thumbnail of The Overview on Toxigenic Fungi and Mycotoxins in Serbia and Montenegro

An Overview on Toxigenic Fungi and Mycotoxins in Europe, 2004

Research paper thumbnail of Microbiological and mycotoxicological correctness of protein feed ingredients in Vojvodina

Zbornik Matice srpske za prirodne nauke, 2011

A l e k s a n d r a S . B o č a r o v S t a n č i ć 1 , N a t a š a M . S a l m a 1 , V l a d i m... more A l e k s a n d r a S . B o č a r o v S t a n č i ć 1 , N a t a š a M . S a l m a 1 , V l a d i m i r R . P a n t i ć 1 , M i l a n J . A d a m o v i ć 2 , A l e k s a n d r a D . M i l j k o v i ć 1 , S v e t l a n a V ABSTRACT: During 2009 and 2010, the microbiological tests of a total of 40 samples of protein feed ingredients (sunflower meal, soybean, soybean cake, soybean grits and soybean meal) originating from Vojvodina were carried out. The most of the samples (57.5%) matched the Serbian regulations on feed. Microbiologically, there was not adequate quality of protein ingredients, which was a consequence of the presence of pathogenic bacteria: Proteus spp. in 12 samples of sunflower meal,

Research paper thumbnail of The role of paecilomyces lilacinus (thom) samson and other fungal species in biodegradation of ochratoxin a

Zbornik Matice srpske za prirodne nauke, 2011

... FEMS Micro-bio Lett, 255: 203-208. B o č aro vS tan č i ć, SA, L evi ć, TJ, D imi ć, RG, S ta... more ... FEMS Micro-bio Lett, 255: 203-208. B o č aro vS tan č i ć, SA, L evi ć, TJ, D imi ć, RG, S tankovi ć, Ž. S., S alma, MN (2009a): Investigation of toxigenic potential of fungal species by the use of simple screening method. Proc. Nat. Sci. Matica Srpska, 116: 25−32. ...

Research paper thumbnail of Mycopopulations of alfalfa silage with particular review on toxigenic Fusarium spp

Zbornik Matice srpske za prirodne nauke, 2005

Research paper thumbnail of Mycotoxins in the silage: Causes of creating, aftermath and protection from acting

Zbornik Matice srpske za prirodne nauke, 2005

Research paper thumbnail of Investigations of the capability of Fusarium isolates from corn for biosynthesis of fusariotoxins

Zbornik Matice srpske za prirodne nauke, 2007

Research paper thumbnail of The toxigenic potential of Fusarium poae originated from wheat

Zbornik Matice srpske za prirodne nauke, 2007

Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were ... more Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight ...

Research paper thumbnail of Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia

Zbornik Matice srpske za prirodne nauke, 2007

Research paper thumbnail of Optimization of laboratory conditions for biosynthesis of type A trichothecenes

Zbornik Matice srpske za prirodne nauke, 2007

A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , V e s n a M. J a ã e v i ã 2 , R a d m i ... more A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , V e s n a M. J a ã e v i ã 2 , R a d m i ABSTRACT: Type A trichothecenes, T-2 toxin and diacetoxyscirpenol -DAS, belong to one of the most toxic groups of fusariotoxins. Although larger quantities of them can be found more often in cooler parts of Europe, regarding their methabolic characteristics and the types of illnesses they provoke, it is obvious that even smaller quantities of these toxins can cause serious health disturbances of humans and animals in climatic conditions of Serbia.

Research paper thumbnail of Fusarium species: The occurrence and the importance in agriculture of Serbia

Zbornik Matice srpske za prirodne nauke, 2009

Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspe... more Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period.

Research paper thumbnail of Investigation of toxigenic potential of fungal species by the use of simple screening method

Zbornik Matice srpske za prirodne nauke, 2009

A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , J e l e n a T. L e v i ã 2 , G o r d a n ... more A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , J e l e n a T. L e v i ã 2 , G o r d a n a R. D i m i ã 3 , S l a v i c a Ÿ. S t a n k o v i ã 2 , N a t a š a M. S a l m a 1

Research paper thumbnail of Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions

Zbornik Matice srpske za prirodne nauke, 2009

Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, or... more Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of F i l t e n b o r g et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO 4 x 5 H 2 O, pH 6.5 (YES Zn ) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs).

Research paper thumbnail of In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate

Zbornik Matice srpske za prirodne nauke, 2011

Research paper thumbnail of Relationships of mycobiota on rachides and kernels of wheat

European Journal of Plant Pathology, 2012

The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14... more The frequency and incidence of fungi, as well as their interdependence, on rachis and grain of 14 wheat cultivars grown under 19 different agroecological conditions in Serbia, were studied. Out of the 23 identified fungal genera, a significantly higher number of species was isolated and identified from rachides (22) than from kernels of wheat (9). Fusarium and Alternaria species were the most frequent (up to 100 %) species on both, rachides and kernels, but the incidence of these fungi were higher on rachides than on kernels. The most frequent of the 14 Fusarium species were F. graminearum (96.8 % on both, rachides and kernels) and F. poae (93.8 % on rachides and 51.6 % on kernels). The frequency of F. verticillioides was significantly higher on rachides (64.5 %) than on kernels (19.4 %). A positive correlation (r00.5356 **) was established between the frequency of F. graminearum on rachides and on kernels. Furthermore, the frequency of Alternaria spp. was also statistically higher on rachides than on kernels, but the correlation was not statistically significant (r00.1729). The incidence of F. graminearum was negatively correlated with the incidence of Alternaria species in both, rachides (r0−0.3783 *) and kernels (r0−0.4863 **). These are the first data on the frequency and incidence of fungi on wheat rachides in Serbia, and they support the few data presented in the world literature. Results of this research could be useful for better understanding of pathways in a fungal infection and the improvement of wheat breeding for resistance, as well as, a proper application of fungicides in the wheat head protection.

Research paper thumbnail of Toxigenic potential Fusarium langsethiae isolates from Serbian wheat kernels

Cereal Research Communications

Research paper thumbnail of Overview on toxigenic fungi and mycotoxins in Serbia and Montenegro

Research paper thumbnail of Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia

Pesticidi i fitomedicina, 2012

A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sun... more A total of 41 species of fungi were isolated from seed samples of barley, maize, soybean, and sunflower collected at different locations in Serbia. The majority of detected species occurred on barley (35 of 41 species or 87.8%) comparing to soybean (17 of 41 species or 41.5%), sunflower (16 of 41 species or 39.0%) and maize (15 of 41 species or 36.9%). Species belonging to genera Alternaria, Chaetomium, Epicoccum, Fusarium, Penicillium and Rhizopus were present on seeds of all four plant species. Alternaria species were dominant on soybean, barley and sunflower seeds (85.7%, 84.7% and 76.9%). F. verticillioides and Penicillium spp. were mainly isolated from maize seeds (100 and 92.3% respectively), while other species were isolated up to 38.5% (Chaetomium spp. and Rhizopus spp.). F. graminearum, F. proliferatum, F. poae and F. sporotrichioides were the most common Fusarium species isolated from barley (51.1-93.3%), while on the soybean seeds F. oxysporum (71.4%), F. semitectum (57.1%) and F. sporotrichioides (57.1%) were prevalent. Frequency of Fusarium species on sunflower seeds varied from 7% (F. equiseti, F. graminearum, F. proliferatum and F. subglutinans) to 15.4% (F. verticillioides). Statistically significant negative correlation (r = -0.678*) was determined for the incidence of F. graminearum and Alternaria spp., as well as, Fusarium spp. and Alternaria spp. (r = -0.614*), on barley seeds. The obtained results revealed that seedborne pathogens were present in most seed samples of important cereals and industrial crops grown under different agroecological conditions in Serbia. Some of the identified fungi are potential producers of mycotoxins, thus their presence is important in terms of reduced food safety for humans and animals. Therefore, an early and accurate diagnosis and pathogen surveillance will provide time for the development and the application of disease strategies.

Research paper thumbnail of The influence of mineral adsorbent „Minazel” on the reduction of residual zearalenone and its metabolites in cow's milk

Veterinarski glasnik, 2003

Izvod: U radu su prikazani rezultati kori{}enja adsorbenta mikotoksina -Minazel, preparata na baz... more Izvod: U radu su prikazani rezultati kori{}enja adsorbenta mikotoksina -Minazel, preparata na bazi prirodnog zeolita, u prevenciji mikotoksikoza i spre~avanju prelaska (akumuliranja) zearalenona i njegovih metabolita u mleku krava. Mikotoksikolo{kim analizama mleka utvr|eno je da je prisustvo zearalenona u mleku (0,053; 0,019 i 0,004 mg/kg) opadalo sa pove}anjem koli~ine Minazela u koncentratu (0; 0,2; 0,5), {to uz ostvareno pove}anje proizvodnje mleka opravdava njegovo kori{}enje.

Research paper thumbnail of An outbreak of Aspergillus species in response to environmental conditions in Serbia

Pesticidi i fitomedicina, 2013

The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wh... more The frequency and incidence of A. flavus and A. niger on barley, maize, soybean, sunflower and wheat grain, the abundance of European corn borer (Ostrinia nubilalis) moths and their interaction depending on weather conditions in the 2008-2012 period were studied. Under the agroecological conditions of Serbia, the species A. niger is more frequent than A. flavus, and concerning the crop species, its frequency is highest in kernels of sunflower, than soybean, maize, barley and wheat. A. flavus was extremely dominant on all plant species in 2012 regarding its frequency: 100% on soybean, 95.3% on maize, 65.2% on barley, 57.1% on sunflower and 45.8% on wheat. Furthermore, the incidence of A. flavus was higher in 2012 than in previous years. The uncommonly high frequency and incidence of A. flavus infestation of maize grain in 2012 were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturity of maize. The precipitation factor (Pf = precipitation sum / average monthly temperature) showed that 2012 was extremely arid in June (Pf = 0.57), July (Pf = 1.45), August (Pf = 0.15) and September (Pf = 1.42). European corn borer (ECB) was a second factor causing intensive occurrence of A. flavus on maize grain in 2012. The maximum flight of ECB moths was recorded as early as in July (5,149) and, as a result of this, high damage and numerous injuries were detected at harvest. Those injuries were covered by visible olive-green powdery colonies typical of A. flavus. In the chronology of A. flavus occurrence, these are the first data on its very high frequency and incidence under the agroecological conditions of Serbia. As intensive infections with A. flavus were rare in the past 50 years, the level of aflatoxins in maize grain was low.

Research paper thumbnail of Influence of Min-a-zel Plus on biochemical, microbiological and mycotoxicological parameters of lucerne silage

Journal of Agricultural Sciences, Belgrade, 2003

Lucerne of the 5 th cut in the budding phase was ensiled in the experiment as fresh and wilted (D... more Lucerne of the 5 th cut in the budding phase was ensiled in the experiment as fresh and wilted (DM= 220 and 360 g/kg), with two degrees of compression (520 and 380 g/dm 3 ) and with added Min-a-zel plus as 2 g/kg green mass. The experiment was performed as the statistical model 2 K .

Research paper thumbnail of The Overview on Toxigenic Fungi and Mycotoxins in Serbia and Montenegro

An Overview on Toxigenic Fungi and Mycotoxins in Europe, 2004

Research paper thumbnail of Microbiological and mycotoxicological correctness of protein feed ingredients in Vojvodina

Zbornik Matice srpske za prirodne nauke, 2011

A l e k s a n d r a S . B o č a r o v S t a n č i ć 1 , N a t a š a M . S a l m a 1 , V l a d i m... more A l e k s a n d r a S . B o č a r o v S t a n č i ć 1 , N a t a š a M . S a l m a 1 , V l a d i m i r R . P a n t i ć 1 , M i l a n J . A d a m o v i ć 2 , A l e k s a n d r a D . M i l j k o v i ć 1 , S v e t l a n a V ABSTRACT: During 2009 and 2010, the microbiological tests of a total of 40 samples of protein feed ingredients (sunflower meal, soybean, soybean cake, soybean grits and soybean meal) originating from Vojvodina were carried out. The most of the samples (57.5%) matched the Serbian regulations on feed. Microbiologically, there was not adequate quality of protein ingredients, which was a consequence of the presence of pathogenic bacteria: Proteus spp. in 12 samples of sunflower meal,

Research paper thumbnail of The role of paecilomyces lilacinus (thom) samson and other fungal species in biodegradation of ochratoxin a

Zbornik Matice srpske za prirodne nauke, 2011

... FEMS Micro-bio Lett, 255: 203-208. B o č aro vS tan č i ć, SA, L evi ć, TJ, D imi ć, RG, S ta... more ... FEMS Micro-bio Lett, 255: 203-208. B o č aro vS tan č i ć, SA, L evi ć, TJ, D imi ć, RG, S tankovi ć, Ž. S., S alma, MN (2009a): Investigation of toxigenic potential of fungal species by the use of simple screening method. Proc. Nat. Sci. Matica Srpska, 116: 25−32. ...

Research paper thumbnail of Mycopopulations of alfalfa silage with particular review on toxigenic Fusarium spp

Zbornik Matice srpske za prirodne nauke, 2005

Research paper thumbnail of Mycotoxins in the silage: Causes of creating, aftermath and protection from acting

Zbornik Matice srpske za prirodne nauke, 2005

Research paper thumbnail of Investigations of the capability of Fusarium isolates from corn for biosynthesis of fusariotoxins

Zbornik Matice srpske za prirodne nauke, 2007

Research paper thumbnail of The toxigenic potential of Fusarium poae originated from wheat

Zbornik Matice srpske za prirodne nauke, 2007

Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were ... more Eleven isolates of F. poae, originated from wheat grain at 9 locations mainly in Vojvodina, were encompassed by the present study. The greatest number of samples was collected in 2005, in which the climatic conditions favoured a more intensive occurrence of Fusarium ear blight ...

Research paper thumbnail of Frequency of toxigenic Fusarium species and fusariotoxins in wheat grain in Serbia

Zbornik Matice srpske za prirodne nauke, 2007

Research paper thumbnail of Optimization of laboratory conditions for biosynthesis of type A trichothecenes

Zbornik Matice srpske za prirodne nauke, 2007

A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , V e s n a M. J a ã e v i ã 2 , R a d m i ... more A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , V e s n a M. J a ã e v i ã 2 , R a d m i ABSTRACT: Type A trichothecenes, T-2 toxin and diacetoxyscirpenol -DAS, belong to one of the most toxic groups of fusariotoxins. Although larger quantities of them can be found more often in cooler parts of Europe, regarding their methabolic characteristics and the types of illnesses they provoke, it is obvious that even smaller quantities of these toxins can cause serious health disturbances of humans and animals in climatic conditions of Serbia.

Research paper thumbnail of Fusarium species: The occurrence and the importance in agriculture of Serbia

Zbornik Matice srpske za prirodne nauke, 2009

Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspe... more Fusarium species have been isolated from over 100 plant species in Serbia. From the economic aspect, they have been and still are the most important for the production and storage of small grains and maize, and are exceptionally important for some other species. Total of 63 species, 35 varieties (var.) and 19 specialised forms (f. sp.) of basic species, particularly of F. oxysporum (4 var. and 12 f. sp.) and F. solani (7 var. and 3 f. sp.) were identified. Species F. langsethiae and F. thapsinum, recently identified, have been isolated from wheat and s o r g h u m seeds, respectively. F. graminearum is the most important pathogen for wheat, barely and maize, while F. poae is also important for wheat and barely. Furthermore, species of the section Liseola (F. verticillioides, F. subglutinans and F. proliferatum) are important for maize and sorghum. In recent years, species of the section Liseola have been increasingly occurring in wheat and barley. The June-October period in Serbia is the most critical period for quality maintenance of stored maize, as the abundance and frequency of fungi, particularly of toxigenic species of the genus Fusarium, are the greatest during that period.

Research paper thumbnail of Investigation of toxigenic potential of fungal species by the use of simple screening method

Zbornik Matice srpske za prirodne nauke, 2009

A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , J e l e n a T. L e v i ã 2 , G o r d a n ... more A l e k s a n d r a S. B o å a r o v -S t a n å i ã 1 , J e l e n a T. L e v i ã 2 , G o r d a n a R. D i m i ã 3 , S l a v i c a Ÿ. S t a n k o v i ã 2 , N a t a š a M. S a l m a 1

Research paper thumbnail of Dynamics of deoxynivalenol and zearalenone production by Fusarium graminearum under laboratory conditions

Zbornik Matice srpske za prirodne nauke, 2009

Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, or... more Toxicological investigations encompassed two cultures of Fusarium graminearum: (i) D2 isolate, originating from air was obtained on Sabouraud medium during a routine control of laboratory sterility conditions at the Department of Microbiology of the Center for Bio-Ecology in 2006, and (ii) GZ-LES control isolate, a well known producer of zearalenone (ZON) and deoxynivalenol (DON), was isolated from maize kernel collected at Leskovac in 1975. Preliminary analysis of fungal potential for the production of DON and ZON were performed by the modified rapid screening method of F i l t e n b o r g et al. (1983). Dynamics of DON and ZON biosynthesis was tested under different conditions of isolate cultivation: (i) in a basic liquid semi-synthetic medium with 2% yeast extract and 15% sucrose, pH 6.5 (YES), (ii) in broth with same concentrations of yeast extract and sucrose supplemented with 0.23 mg/l ZnSO 4 x 5 H 2 O, pH 6.5 (YES Zn ) and (iii) on natural solid substrates such as wet sterilized maize and rice kernels. The quantitative determination of DON and ZON was performed in both liquid and natural solid substrates with thin-layer chromatographic methods (TLCs).

Research paper thumbnail of In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate

Zbornik Matice srpske za prirodne nauke, 2011