satyendra singh | Banaras Hindu University, Varanasi (original) (raw)

Papers by satyendra singh

Contemporary Clinical Dentistry, 2015

Orofacial granulomatosis (OFG) is a rare disorder affecting the orofacial region, and clinically ... more Orofacial granulomatosis (OFG) is a rare disorder affecting the orofacial region, and clinically characterized by diffuse, nontender, soft to firm, painless swelling restricted to one or both lips and intraoral sites such as tongue, gingiva and buccal mucosa. Histologically, OFG is characterized by noncaseating granulomatous inflammation. The early diagnosis of OFG is essential for the better prognosis of the lesion. Delay in diagnosis of OFG results into formation of indurated and permanent swelling of the lip that not only compromises esthetic appearance but also causes impairment in function such as speaking and eating. Early diagnosis of OFG is challenging to the health care professionals due to clinical and histological resemblance to other chronic granulomatous disorders. Thus, dentists may act as a first person to diagnose the lesion and play an important role in the multidisciplinary treatment of granulomatous disorders. Here, we present a case of OFG affecting lips and gingiva in a 15-year-old patient without any identifiable systemic or local causes.

J Mol Biol, 1995

Energy-dependent protein degradation is carried out by large multimeric protein complexes such as... more Energy-dependent protein degradation is carried out by large multimeric protein complexes such as the proteasomes of eukaryotic and archaeal cells and the ATP-dependent proteases of eubacterial cells. Clp protease, a major multicomponent protease ofEscherichia coli, consists of a proteolytic component, ClpP, in association with an ATP-hydrolyzing, chaperonin-like component, ClpA. To provide a structural basis for understanding the regulation and mechanism of action of Clp protease, we have used negative staining electron microscopy and image analysis to examine ClpA and ClpP separately, as well as active ClpAP complexes. Digitized images of ClpP and ClpA were analyzed using a novel algorithm designed to detect rotational symmetries. ClpP is composed of two rings of seven subunits superimposed in bipolar fashion along the axis of rotational symmetry. This structure is similar to that formed by the beta subunits of the eukaryotic and archaeal proteasomes. In the presence of MgATP, ClpA forms an oligomer with 6-fold symmetry when vieweden face. Side views of ClpA indicate that the subunits are bilobed with the respective domains forming two stacked rings. ClpAP complexes contain a tetradecamer of ClpP flanked at one or both ends with a hexamer of ClpA, resulting in a symmetry mismatch between the axially aligned molecules. Our findings demonstrate that, despite the lack of sequence similarity between ClpAP and proteasomes, these multimeric proteases nevertheless have a profound similarity in their underlying architecture that may reflect a common mechanism of action.

Journal of Physics E Scientific Instruments, Nov 29, 2000

Experimental Parasitology, Apr 8, 2013

Neck muscle, eyelid, and tongue were examined for diagnosis of swine cysticercosis.In house ELISA... more Neck muscle, eyelid, and tongue were examined for diagnosis of swine cysticercosis.In house ELISA was developed as sero-diagnosis tool.ELISA with crude lysate is a good diagnostic tool for swine cysticercosis.Neck muscle, eyelid and tongue examination can also be used for screening of swine.Swine cysticercosis is very common in the developing countries where pigs are raised. Undercooked measly pork consumption leads to taeniasis; Taenia carriers act as source of human and swine cysticercosis and neurocysticercosis. Diagnosis of swine cysticercosis is important to break the cycle of disease transmission. The present study compared the neck muscle, tongue and eye examinations, and serum ELISA with different preparations (crude lysate, cyst fluid, scolex and cyst wall antigens) of Taenia solium cyst for the diagnosis of swine cysticercosis. Total of 24 pigs initially identified by neck muscle, tongue and eyelid examinations were purchased from local slaughter house and subjected to MRI for confirmation of cysticercosis. Sera from 20 MRI confirmed infected pigs and 50 disease free controls were subjected to ELISA with T. solium cyst antigens. Neck muscle examination was 100% sensitive and 75% specific for the diagnosis of swine cysticercosis, whereas tongue and eye examinations were 70% and 25% sensitive, respectively. ELISA with crude lysate had 85% sensitivity and 98% specificity. ELISA with cyst fluid, scolex and cyst wall antigens showed 70%, 65%, and 45% sensitivity, respectively. The present study showed that neck muscle examination was highly sensitive but less specific, while ELISA with crude antigens had reasonable sensitivity and high specificity for diagnosis of swine cysticercosis. ELISA with crude lysate can be used as a screening tool for swine infection.

Http Dx Doi Org 10 1080 01431168908903922, Apr 27, 2007

The aims of this presentation were (i) to simulate the solar zenith angle effect on the Global Ve... more The aims of this presentation were (i) to simulate the solar zenith angle effect on the Global Vegetation Index (GVI), (ii) to derive an expression for removing such an effect from the GVI data by the above simulation procedure and (iii) to apply this relation to the GVI data obtained from the NOAA-AVHRR imagery.

The Journal of Biological Chemistry, Jul 2, 1999

ClpA, a member of the Clp/Hsp100 family of ATPases, is both an ATP-dependent molecular chaperone ... more ClpA, a member of the Clp/Hsp100 family of ATPases, is both an ATP-dependent molecular chaperone and the regulatory component of ClpAP protease. We demonstrate that chaperone and protease activities occur concurrently in ClpAP complexes during a single round of RepA binding to ClpAP and ATP-dependent release. This result was substantiated with a ClpA mutant, ClpA(K220V), carrying an amino acid substitution in the N-terminal ATP binding site. ClpA(K220V) is unable to activate RepA, but the presence of ClpP or chemically inactivated ClpP restores its ability to activate RepA. The presence of ClpP simultaneously facilitates degradation of RepA. ClpP must remain bound to ClpA(K220V) for these effects, indicating that both chaperone and proteolytic activities of the mutant complex occur concurrently. ClpA(K220V) itself is able to form stable complexes with RepA in the presence of a poorly hydrolyzed ATP analog, adenosine 5-O-(thiotriphosphate), and to release RepA upon exchange of adenosine 5-O-(thiotriphosphate) with ATP. However, the released RepA is inactive in DNA binding, indicating that the N-terminal ATP binding site is essential for the chaperone activity of ClpA. Taken together, these results suggest that substrates bound to the complex of the proteolytic and ATPase components can be partitioned between release/reactivation and translocation/degradation.

Http Dx Doi Org 10 1080 01431168508948516, Jul 6, 2010

It has been established that the sea-surface brightness temperatures Tb4 in the 11 μ m channel an... more It has been established that the sea-surface brightness temperatures Tb4 in the 11 μ m channel and Tb4in the 12 μ m channel of the Advanced Very High Resolution Radiometer (AVHRR/ 2) are linearly related to a good degree of accuracy, i.e. Tb5= α+ β Tb4 Using AVHRR/ 2 data for various dates and from different parts of the world's oceans, the parameters a and 0 have been determined. The above relation may then be used for simulating Tb5 for those cases for which only Tb4 is available (e.g. for the AVHRR on TIROS-N, NOAA-6, NOAA-8, etc.). The brightness temperature TM and pseudo-brightness temperature Tb5 then enable one to use the split-window technique for estimating atmospherically-corrected sea-surface temperatures (SSTs) from the 11μ m channel data alone. Such an atmospheric correction technique should be a possibility because the 11μ m channel of the AVHRR on the various satellites in question are almost identicalThis technique has been used with two split-window algorithms for correcting the data from the 11μ m channel of the AVHRR instrument on the TIROS-N satellite obtained off south-western Portugal. One of the algorithms gives ‘ skin’ temperatures and the other algorithm gives bulk temperatures. The resulting SSTs for twelve dates from 15 June 1979 to 14 June 1980 have been compared with sea-surface (skin) temperatures which were obtained with airborne radiometer data obtained on the same dates.

Int J Remote Sens, 1986

... 466 J. R. Eyre ... with SST; correction for some of the uncorrelated components requires eith... more ... 466 J. R. Eyre ... with SST; correction for some of the uncorrelated components requires either additional channels or external information such as forecast atmo-spheric temperature/humidity profiles, as used in the METEOSAT operational SST retrieval method (Campbell 1982).A ...

Synthesis Stuttgart, 1993

short paper, Synthesis 1993; 1993: 468-470 DOI: 10.1055/s-1993-25882. © Georg Thieme Verlag, Rüdi... more short paper, Synthesis 1993; 1993: 468-470 DOI: 10.1055/s-1993-25882. © Georg Thieme Verlag, Rüdigerstr. 14, 70469 Stuttgart, Germany. All rights reserved. This journal, including all individual contributions and illustrations ...

Chinese Journal of Physics Taipei, 2009

Title: Electrical Transport and Phase Transition Study of Solid Electrolyte Li3VO4 : x Li3PO4. Au... more Title: Electrical Transport and Phase Transition Study of Solid Electrolyte Li3VO4 : x Li3PO4. Authors: Singh, Vishalakshi; Singh, Satyendra; Gaur, Kanchan. Publication: Chinese Journal of Physics, vol. 47, Issue 1, p.61. Publication Date: 02/2009. Origin: WEB. ...

Syn Reactiv Inorg Metal Org C, 2002

Communications of the Association For Information Systems, 2005

... [Dover 2004] And, according to the focus group, adoption can depend heavily on the initial da... more ... [Dover 2004] And, according to the focus group, adoption can depend heavily on the initial dashboard launch. One company used a pilot approach to building and introducing its dashboards. ... However you introduce dashboard technology, it is important to get it right the first ...

Veterinary Parasitology, 2015

Neurocysticercosis (NCC) is a parasitic infection of central nervous system (CNS). Expression of ... more Neurocysticercosis (NCC) is a parasitic infection of central nervous system (CNS). Expression of adhesion molecules, chemokines and matrix metalloproteinases (MMPs) were investigated on brain tissues surrounding viable (n=15) and degenerating cysticerci (n=15) of Taenia solium in swine by real-time RT-PCR and ELISA. Gelatin gel zymography was performed for MMPs activity. ICAM-1 (intercellular adhesion molecule-1), E-selectin, MIP-1α (macrophage inflammatory protein-1α), Eotaxin-1 and RANTES (regulated on activation, normal T cell expressed and secreted) were associated with degenerating cysticerci (cysts). However, VCAM-1 (vascular cell adhesion molecule-1), MCP-1 (monocyte chemotactic protein-1), MMP-2 and MMP-9 were associated with both viable and degenerating cysts. In conclusion, viable and degenerating cysticerci have different immune molecule profiles and role of these molecules in disease pathogenesis needs to be investigated.

Microbial Drug Resistance, 2015

Heterogeneous vancomycin intermediate Staphylococcus aureus (hVISA) strains are increasingly repo... more Heterogeneous vancomycin intermediate Staphylococcus aureus (hVISA) strains are increasingly reported, and their association with vancomycin treatment failure is a well-known problem worldwide. A total of 500 clinical isolates of methicillin-resistant S. aureus were screened for hVISA by four different methods from May 2011 to May 2014. The number of strains screened for hVISA from May to April in 2011-2012, 2012-2013, and 2013-2014 were 198, 123, and 179, respectively. hVISA strains were studied by transmission electron microscopy (TEM) for cell wall thickness and also for their ability to form biofilm on a polystyrene microtiter plate. hVISA strains detected by four different methods-brain heart infusion agar with vancomycin with 4 mg/L/gradient plate/macro E-test/and glycopeptide resistance detection (GRD) E test-were as follows: 11.6%/10%/9%, and 9.5% in 2011-2012, 12.1%/9.7%/8.9%, and 10.5% in 2012-2013, and 13.9%/11.7%/11.1%, and 12.8% in 2013-2014, respectively. Population analysis profile-area under curve analysis confirmed hVISA in 4.5% (9/198), 6.5% (8/123), and 6.7% (12/179) in respective years; 24% (7/29) of hVISA isolates were nonsusceptible to daptomycin. TEM showed a significant increase in cell wall thickness of hVISA isolates (p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;0.001) with a distinct reduction in their biofilm formation ability.

Antimicrobial Agents and Chemotherapy, 2015

Antimicrobial effects of copper ions and salts are well known, but the effects of cuprous oxide n... more Antimicrobial effects of copper ions and salts are well known, but the effects of cuprous oxide nanoparticles (Cu2O-NPs) on staphylococcal biofilm have not yet been clearly revealed. The present study evaluated Cu2O-NPs for antibacterial and antibiofilm activities against heterogeneous vancomycin intermediate Staphylococcus aureus (hVISA) and vancomycin intermediate S. aureus (VISA). Nanoscaled cuprous oxide (Cu2O), generated by solution phase technology, contained Cu2O octahedral nanoparticles. Field emission electron microscopy demonstrated particles size ranging from 100-150 nm. Cu2O-NPs inhibited the growth of S. aureus, and showed antibiofilm activity. The minimum inhibitory concentrations and minimum biofilm inhibitory concentrations ranged from 625 μg/mL to 5000 μg/mL and 2500 μg/ml to 10,000 μg/ml, respectively. Exposure of S. aureus to Cu2O-NPs caused leakage of cellular constituents and increased uptake of ethidium bromide and propidium iodide. Exposure also caused significant reduction in overall vancomycin-bodipy (dipyrromethene boron difluoride [4,4-difluoro-4-bora-3a,4a-diazas- indacene] fluorescent dye) binding, and decrease in viable cell counts in presence of 7.5% sodium chloride. Cu2O-NPs toxicity assessment by haemolysis assay showed no cytotoxicity at 625 - 10000 μg/ml concentrations. The results suggest that Cu2O-NPS exert their action by disruption of bacterial cell membrane and can be used as effective antistaphylococcal and antibiofilm agents in diverse medical devices.