DoCl1 (S+L-) (original) (raw)

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DoCl1 (S+L-) (dog, kidney)

SPECIES: dog
TISSUE/ORGAN: kidney

ECACC catalog code: 90102523
Morphology: Epithelial
TRANSFORMED BY: murine sarcoma virus
Description: Canine kidney, Moloney Sarcoma Virus infected
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Murine MSV, RNA and xenotropic human tumour viruses

Available in the following LABORATORY:

• Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
  McCoy's 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/0.02% EDTA; 5% CO2; 37C.
  Passages: 12; Karyotype: 2n = 78
  Derived by the infection of a sub-clone of MDCK (ECACC No. 84121903) with murine MSV. The cells are used for studies of the interaction between RNA tumour viruses and mammalian cells including the isolation of xenotropic human tumour virus.
  Research council deposit: No; Price Code: C;
  Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 34.1

Bibliographic references:

• Virology 1975;67:344 - PMID: 52940
• Virology 1976;70:313 - PMID: 57666