Patrick Rouimi | Université de Toulouse III Paul Sabatier (original) (raw)

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Papers by Patrick Rouimi

Research paper thumbnail of Electrospray ionization-mass spectrometry as a tool for characterization of glutathione S-transferase isozymes

HAL (Le Centre pour la Communication Scientifique Directe), 1995

A method is described to study the precise positioning of transmembrane peptides in a phospholipi... more A method is described to study the precise positioning of transmembrane peptides in a phospholipid bilayer combining hydrogen/deuterium (H/D) exchange and nanoelectrospray ionization mass spectrometry. The method was tested by using model systems consisting of designed α-helical transmembrane peptides [acetylGW 2 (LA) 5 W 2 Aethanolamine (WALP16) and acetyl-(GA) 3 W 2 (LA) 5 W 2 (AG) 3 ethanolamine (WALP16(+10))] incorporated in large unilamellar vesicles of 1,2-dimyristoyl- sn -glycero-3-phosphocholine. Both peptides consist of an alternating leucine/alanine hydrophobic core sequence flanked by tryptophan residues as interfacial anchor residues. In the case of WALP16(+10), this sequence is extended at both ends by 5-aa glycine/alanine tails extending into the aqueous phase surrounding the bilayer. H/D exchange of labile hydrogens in these peptides was monitored in time after dilution of the vesicles in buffered deuterium oxide. It was found that the peptides can be measured by direct introduction of the proteoliposome suspension into the mass spectrometer. Several distinct H/D exchange rates were observed (corresponding to half-life values varying from ≤2 to ≈2 × 10 4 min). Fast exchange rates were assigned to the water-exposed tails of WALP16(+10). For both WALP16 and WALP16(+10), intermediate exchange rates were assigned to the residues close to the membrane/water interface, and the slow exchange rates to the membrane-embedded hydrophobic core. These assignments were confirmed by results from collision-induced dissociation tandem mass spectrometry experiments, which allowed analysis of exchange of individual peptide amide linkages. This proteoliposome nanoelectrospray ionization mass spectrometry technique is shown to be an extremely sensitive and powerful tool for revealing site-specific information on peptide–membrane interactions.

Research paper thumbnail of Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts

Biochemical Journal, Aug 15, 2001

Research paper thumbnail of Projet Euromix. Vers une démarche normalisée, à l’échelle européenne, pour évaluer les risques des mélanges de contaminants chimiques

HAL (Le Centre pour la Communication Scientifique Directe), 2019

Research paper thumbnail of Micronutrients and cancer prevention: effect of sulphoraphane, an isothiocyanate from broccoli, in the regulation of human colon cancerous cell growth and death

HAL (Le Centre pour la Communication Scientifique Directe), 2002

Research paper thumbnail of Growth arrest and cell death induced by the dietary anticarcinogen sulforaphane in human renal cancer cells are maintained after it was conjugated to glutathione

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Cell death and the glutathione S-transferase alpha overexpression induced by the natural anticarcinogen sulforaphane in Caco2 cells follow independent pathways

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts

Biochemical Journal, Aug 8, 2001

A cytosolic glutathione S-transferase (GST, EC 2.5.1.18) from the recently characterized Omega cl... more A cytosolic glutathione S-transferase (GST, EC 2.5.1.18) from the recently characterized Omega class [GSTO; Board et al. 2000, J. Biol. Chem. 275, 24798–24806] has been identified in pig organs. It was found widely distributed in the different tissues investigated and especially abundant in liver and muscle. The hepatic enzyme has been purified to homogeneity by using its selective affinity for S-hexylglutathione over GSH, thus providing a simple method to isolate mammalian GSTO. The dimeric protein has a subunit molecular mass of 27328Da as measured by electrospray ionization MS. Internal peptide sequencing and complete cDNA sequencing revealed strong similarities with its human recombinant orthologue and two rodent GST-like proteins with the ability to catalyse the GSH-dependent reduction of dehydroascorbate. Additional similarities, including the presence of a specific N-terminal extension and of immunological cross-reactivity, support the results. Moreover, this gene encoding GSTO generates two organ-specific transcripts, suggesting transcriptional mechanisms with a significance that is as yet uncharacterized.

Research paper thumbnail of Chevolleau, S., Rouimi, P. et Tulliez, J. Séparation des sous-unités de glutathion S-transférases par électrophorèse capillaire

HAL (Le Centre pour la Communication Scientifique Directe), 1995

Research paper thumbnail of Rouimi P, Marcelin M, Fricaux T, Payrastre L (2005-11). Utilisation de membranes de macroarray dédiées à l'étude des voies de signalisation cellulaires modulées par le sulforaphane, isothiocyanate issu du brocoli

HAL (Le Centre pour la Communication Scientifique Directe), Nov 8, 2005

Research paper thumbnail of Ledger TN, Pinton P, Bourges D, Roumi P, Salmon H, Oswald IP (2004). Development of a macroarray to specifically analyze immunological gene expression in swine

HAL (Le Centre pour la Communication Scientifique Directe), 2004

Research paper thumbnail of Sanchez-Rodrigez R, Rouimi P, Perez-Carreon JI. Prostaglandin reductase 1 expression is increased in hepatocellular carcinomas from clinical cases and experimental tumors in rat

HAL (Le Centre pour la Communication Scientifique Directe), Jun 26, 2014

Research paper thumbnail of M. Pérez-Lopez, P. Rouimi, L. Debrauwer and Cravedi, J.P. Glutathione S-transferase subunits pattern in rainbow trout isolated hepatocytes

HAL (Le Centre pour la Communication Scientifique Directe), 1997

Research paper thumbnail of Roumi P, Marcellin M, Fricaux T et Gamet-Payrastre L. Utilisation de macroarrays dédiés dans l'étude de la modulation des voies de signalisation cellulaires par le sulforaphane, isothiocyanate du brocoli

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Micronutriments, contaminants alimentaires et signalisation cellulaire liée au cancer

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Ferté, M.P., Debrauwer, L., Pérez-Lopez, M., Rouimi, P. et Cravedi, J.P. Caractérisation de glutathione S-transférases de truite en spectrométrie de masse avec ionisation par electrospray

HAL (Le Centre pour la Communication Scientifique Directe), 1996

Research paper thumbnail of Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response

Cellular and Molecular Life Sciences, Jul 25, 2021

Research paper thumbnail of Electrospray Ionization-Mass Spectrometry as a Tool for Characterization of Glutathione S-Transferase Isozymes

Analytical Biochemistry, Aug 1, 1995

Reversed-phase high-performance liquid chromatography coupled to electrospray ionization-mass spe... more Reversed-phase high-performance liquid chromatography coupled to electrospray ionization-mass spectrometry (ESI-MS) was used for the first time to determine the molecular masses of nine rat liver cytosolic glutathione S-transferase (GST) subunits. The precision of the measurements was ±3-4 mass units which, in practice, allowed discrimination between monomers differing by more than 8 Da. Mass accuracy was improved by replicates in the

Research paper thumbnail of Rouimi P, Anglade P, Debrauwer L, Tulliez J. Characterizaion of pig liver cytosolic glutathione S-transferase using HPLC-electrospray ionization mass spectrometry

HAL (Le Centre pour la Communication Scientifique Directe), Apr 22, 1995

Research paper thumbnail of Gaillard L, Gasc N, Tulliez J, Gamet-Payrastre L, Rouimi P. Etude des effets de l'administration intracolique d'un micronutriment du brocoli, le sulforaphane, sur la cancérogenèse induite chez le rat

HAL (Le Centre pour la Communication Scientifique Directe), Jan 23, 2003

Research paper thumbnail of Patrick Rouimi, Jacques Tulliez, Laurence Gamet-Payrastre. Sulforaphane induces glutathione-S-transferase alpha in subconfluent Caco-2 colon cancer cells

HAL (Le Centre pour la Communication Scientifique Directe), May 19, 2000

Research paper thumbnail of Electrospray ionization-mass spectrometry as a tool for characterization of glutathione S-transferase isozymes

HAL (Le Centre pour la Communication Scientifique Directe), 1995

A method is described to study the precise positioning of transmembrane peptides in a phospholipi... more A method is described to study the precise positioning of transmembrane peptides in a phospholipid bilayer combining hydrogen/deuterium (H/D) exchange and nanoelectrospray ionization mass spectrometry. The method was tested by using model systems consisting of designed α-helical transmembrane peptides [acetylGW 2 (LA) 5 W 2 Aethanolamine (WALP16) and acetyl-(GA) 3 W 2 (LA) 5 W 2 (AG) 3 ethanolamine (WALP16(+10))] incorporated in large unilamellar vesicles of 1,2-dimyristoyl- sn -glycero-3-phosphocholine. Both peptides consist of an alternating leucine/alanine hydrophobic core sequence flanked by tryptophan residues as interfacial anchor residues. In the case of WALP16(+10), this sequence is extended at both ends by 5-aa glycine/alanine tails extending into the aqueous phase surrounding the bilayer. H/D exchange of labile hydrogens in these peptides was monitored in time after dilution of the vesicles in buffered deuterium oxide. It was found that the peptides can be measured by direct introduction of the proteoliposome suspension into the mass spectrometer. Several distinct H/D exchange rates were observed (corresponding to half-life values varying from ≤2 to ≈2 × 10 4 min). Fast exchange rates were assigned to the water-exposed tails of WALP16(+10). For both WALP16 and WALP16(+10), intermediate exchange rates were assigned to the residues close to the membrane/water interface, and the slow exchange rates to the membrane-embedded hydrophobic core. These assignments were confirmed by results from collision-induced dissociation tandem mass spectrometry experiments, which allowed analysis of exchange of individual peptide amide linkages. This proteoliposome nanoelectrospray ionization mass spectrometry technique is shown to be an extremely sensitive and powerful tool for revealing site-specific information on peptide–membrane interactions.

Research paper thumbnail of Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts

Biochemical Journal, Aug 15, 2001

Research paper thumbnail of Projet Euromix. Vers une démarche normalisée, à l’échelle européenne, pour évaluer les risques des mélanges de contaminants chimiques

HAL (Le Centre pour la Communication Scientifique Directe), 2019

Research paper thumbnail of Micronutrients and cancer prevention: effect of sulphoraphane, an isothiocyanate from broccoli, in the regulation of human colon cancerous cell growth and death

HAL (Le Centre pour la Communication Scientifique Directe), 2002

Research paper thumbnail of Growth arrest and cell death induced by the dietary anticarcinogen sulforaphane in human renal cancer cells are maintained after it was conjugated to glutathione

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Cell death and the glutathione S-transferase alpha overexpression induced by the natural anticarcinogen sulforaphane in Caco2 cells follow independent pathways

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts

Biochemical Journal, Aug 8, 2001

A cytosolic glutathione S-transferase (GST, EC 2.5.1.18) from the recently characterized Omega cl... more A cytosolic glutathione S-transferase (GST, EC 2.5.1.18) from the recently characterized Omega class [GSTO; Board et al. 2000, J. Biol. Chem. 275, 24798–24806] has been identified in pig organs. It was found widely distributed in the different tissues investigated and especially abundant in liver and muscle. The hepatic enzyme has been purified to homogeneity by using its selective affinity for S-hexylglutathione over GSH, thus providing a simple method to isolate mammalian GSTO. The dimeric protein has a subunit molecular mass of 27328Da as measured by electrospray ionization MS. Internal peptide sequencing and complete cDNA sequencing revealed strong similarities with its human recombinant orthologue and two rodent GST-like proteins with the ability to catalyse the GSH-dependent reduction of dehydroascorbate. Additional similarities, including the presence of a specific N-terminal extension and of immunological cross-reactivity, support the results. Moreover, this gene encoding GSTO generates two organ-specific transcripts, suggesting transcriptional mechanisms with a significance that is as yet uncharacterized.

Research paper thumbnail of Chevolleau, S., Rouimi, P. et Tulliez, J. Séparation des sous-unités de glutathion S-transférases par électrophorèse capillaire

HAL (Le Centre pour la Communication Scientifique Directe), 1995

Research paper thumbnail of Rouimi P, Marcelin M, Fricaux T, Payrastre L (2005-11). Utilisation de membranes de macroarray dédiées à l'étude des voies de signalisation cellulaires modulées par le sulforaphane, isothiocyanate issu du brocoli

HAL (Le Centre pour la Communication Scientifique Directe), Nov 8, 2005

Research paper thumbnail of Ledger TN, Pinton P, Bourges D, Roumi P, Salmon H, Oswald IP (2004). Development of a macroarray to specifically analyze immunological gene expression in swine

HAL (Le Centre pour la Communication Scientifique Directe), 2004

Research paper thumbnail of Sanchez-Rodrigez R, Rouimi P, Perez-Carreon JI. Prostaglandin reductase 1 expression is increased in hepatocellular carcinomas from clinical cases and experimental tumors in rat

HAL (Le Centre pour la Communication Scientifique Directe), Jun 26, 2014

Research paper thumbnail of M. Pérez-Lopez, P. Rouimi, L. Debrauwer and Cravedi, J.P. Glutathione S-transferase subunits pattern in rainbow trout isolated hepatocytes

HAL (Le Centre pour la Communication Scientifique Directe), 1997

Research paper thumbnail of Roumi P, Marcellin M, Fricaux T et Gamet-Payrastre L. Utilisation de macroarrays dédiés dans l'étude de la modulation des voies de signalisation cellulaires par le sulforaphane, isothiocyanate du brocoli

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Micronutriments, contaminants alimentaires et signalisation cellulaire liée au cancer

HAL (Le Centre pour la Communication Scientifique Directe), 2005

Research paper thumbnail of Ferté, M.P., Debrauwer, L., Pérez-Lopez, M., Rouimi, P. et Cravedi, J.P. Caractérisation de glutathione S-transférases de truite en spectrométrie de masse avec ionisation par electrospray

HAL (Le Centre pour la Communication Scientifique Directe), 1996

Research paper thumbnail of Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response

Cellular and Molecular Life Sciences, Jul 25, 2021

Research paper thumbnail of Electrospray Ionization-Mass Spectrometry as a Tool for Characterization of Glutathione S-Transferase Isozymes

Analytical Biochemistry, Aug 1, 1995

Reversed-phase high-performance liquid chromatography coupled to electrospray ionization-mass spe... more Reversed-phase high-performance liquid chromatography coupled to electrospray ionization-mass spectrometry (ESI-MS) was used for the first time to determine the molecular masses of nine rat liver cytosolic glutathione S-transferase (GST) subunits. The precision of the measurements was ±3-4 mass units which, in practice, allowed discrimination between monomers differing by more than 8 Da. Mass accuracy was improved by replicates in the

Research paper thumbnail of Rouimi P, Anglade P, Debrauwer L, Tulliez J. Characterizaion of pig liver cytosolic glutathione S-transferase using HPLC-electrospray ionization mass spectrometry

HAL (Le Centre pour la Communication Scientifique Directe), Apr 22, 1995

Research paper thumbnail of Gaillard L, Gasc N, Tulliez J, Gamet-Payrastre L, Rouimi P. Etude des effets de l'administration intracolique d'un micronutriment du brocoli, le sulforaphane, sur la cancérogenèse induite chez le rat

HAL (Le Centre pour la Communication Scientifique Directe), Jan 23, 2003

Research paper thumbnail of Patrick Rouimi, Jacques Tulliez, Laurence Gamet-Payrastre. Sulforaphane induces glutathione-S-transferase alpha in subconfluent Caco-2 colon cancer cells

HAL (Le Centre pour la Communication Scientifique Directe), May 19, 2000

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