Simon Tinman | Bar-Ilan University (original) (raw)

Papers by Simon Tinman

Laboratory Animals, Dec 4, 2023

Chemotherapy, 1994

Mice infected with Brucella melitensis were treated with azithromycin or roxithromycin at a dose ... more Mice infected with Brucella melitensis were treated with azithromycin or roxithromycin at a dose of 50 mg/kg/day i.p. alone and in combination with streptomycin 75 mg/kg/day for 14 days. Streptomycin at this dose was previously documented to be ineffective against murine brucellosis. Azithromycin- and azithromycin/streptomycin-treated animals demonstrated a significantly better cure rate than controls. Therapy failure was observed in all mice treated with roxithromycin 50 mg/kg/day i.p. alone or in combination with streptomycin 75 mg/kg/day. Our findings demonstrate that azithromycin cures experimental murine brucellosis and may be an effective alternative in the therapy of human brucellosis.

O. mossambicus). Yaniv Palti 1* , Simon Tinman 2 , Avner Cnaani 1 , Yaakov Avidar 3 , Micha Ron 1... more O. mossambicus). Yaniv Palti 1* , Simon Tinman 2 , Avner Cnaani 1 , Yaakov Avidar 3 , Micha Ron 1 ,. and Gideon Hulata 1. 1. Institute of Animal Science, Agricultural Research Organization, PO Box 6, Bet Dagan 50250, Israel. ...

(A) Average body weight of WT and KO male (left panel) and female (right panel) mice. In... more (A) Average body weight of WT and KO male (left panel) and female (right panel) mice. In both genders, the weight of SIRT6 KO mice was significantly lower as compared with age-matched WT animals. (B) KO mice showed a trend towards lower percentage of total body fat in comparison to their WT littermates. The percentage of total body fat was measured at 6–8 months of age. Results are presented as mean ± SEM. n = 3–14 per group.</p

Scotopic (A-F) and photopic (G, H) full-field electroretinography (ERG) recordings may s... more Scotopic (A-F) and photopic (G, H) full-field electroretinography (ERG) recordings may suggest accelerated aging in KO eyes. At one month of age, dark-adapted ERG responses to a series of increasing white flash intensities showed preserved a-wave amplitudes in KO as compared to WT and HET eyes (A), while a trend towards lower amplitudes was observed in the scotopic b-wave amplitudes, attaining statistical significance at 3 high intensities (B). At the age of 6 months, scotopic a-wave responses at higher intensities showed a trend towards lower amplitudes (C), and b-wave amplitudes were markedly reduced (D). At 10 months, both a- and b-wave amplitudes in KO mice were markedly reduced as compared to WT and HET mice (E, F). Light adapted 16Hz flicker responses were also markedly reduced in KO mice at 6 and 10 months (G, H). ERG responses were similar in WT and HET mice at all ages. Results are presented as mean ± SEM; n = 6–9 for WT, n = 7–8 for HET, and n = 4–5 for KO mice. * P < 0.05.</p

Glucose tolerance test (GTT) in WT and KO male (A) and female (B) mice. Area under curve... more Glucose tolerance test (GTT) in WT and KO male (A) and female (B) mice. Area under curve (AUC) is shown on the right for each GTT. Both male and female KO mice show increased glucose uptake in comparison to WT mice. (C) Insulin tolerance test in male (left panel) and female (right panel) mice showed no significant difference between genotypes. (D) Insulin blood levels after glucose stimulation as determined by ELISA. All assays were done at 3–4 months old. Results are presented as mean ± SEM. n = 5–14 per group. * P < 0.05, ** P < 0.01</p

Israeli Journal of Aquaculture-bamidgeh, 2000

(A-B) Photographs and histological sections of corneas in 10-month-old WT and KO mice. C... more (A-B) Photographs and histological sections of corneas in 10-month-old WT and KO mice. Corneas of normal WT mice are fully transparent, with no ingrowth of blood vessels (A, left panel). In contrast, from 6 months of age, corneal scarring and vascularization were often present in KO mice eyes (A, right panel). Histological section in KO mice (B, lower panel) shows corneal thickening, stromal edema, inflammation and scarring that are not present in WT mice of the same age (B, upper panel). B: Paraffin embedded sections stained with hematoxylin and eosin; C- Cornea; L-Lens; original magnification x10; Scale bar = 200μm.</p

(A) Relative expression of GLUT1 in MEF's, liver and muscle of WT... more (A) Relative expression of GLUT1 in MEF's, liver and muscle of WT and KO male mice as determined by quantitative real-time PCR. (B) Serum IGF-1 levels of WT and KO male and female mice. All measurements were done at 6–8 months of age. Results are presented as mean ± SEM. n = 5–14 per group. * P < 0.05.</p

1. Hair is emerging as a popular tool to examine steroid hormone levels in wild mammals. The reli... more 1. Hair is emerging as a popular tool to examine steroid hormone levels in wild mammals. The reliability of this approach, however, depends on an understanding of steroid hormone incorporation into hair as well as appropriate validations. 2. We reviewed studies that have examined steroid hormones in wildlife hair with the goal of summarizing the analytical, physiological, and biological evidence that this approach is meaningful. Accordingly, we differentiated among validations aimed at evaluating the reliability of the analytical method versus those designed to assess whether hormone levels in hair reflect physiologically meaningful processes in the target species. 3. Our literature survey revealed that endogenous steroids have been examined in hair from 42 species of non-human animals across 7 mammalian classes. Although the majority (85%) of 72 studies reported analytical validations of the method, physiological validations have only been reported for five species. Moreover, results of physiological validations were inconsistent among studies, highlighting the need for further research designed carefully to differentiate among the multiple purported models of steroid incorporation into hair in species with different types of hair and hair growth patterns. 4. To complement our review of published studies, we present new data supporting a positive relationship between levels of the steroid, cortisol, in hair and blood across eight mammalian species. In addition, we present novel results from a laboratory-based study showing variable hair growth in genetically identical laboratory mice that were kept under controlled conditions. 5. Synthesis and Applications: Collectively, this synthesis reveals substantial progress towards the validation of endocrine assays in hair from a variety of wildlife species. Further validations of other steroids, combined with appropriate physiological validations, would expand the potential applications of hair testing in wildlife research. As a key example, physiological data can provide mechanistic insight into species’ responses to change and may therefore contribute to conservation planning

Genotypes of progeny from crosses of F1 SIRT6+/− mice.</p

Kaplan—Meier survival curves of WT, HET and KO male (left panel) and female (right panel... more Kaplan—Meier survival curves of WT, HET and KO male (left panel) and female (right panel) mice in 129/SvJ/BALB/c background. 90% of KO male mice died before 200 days of age and more than 75% of KO female mice survived over 300 days of age. Males, n = 23–39 per group; Females, n = 14–40 per group.</p

Vaccine, 2003

We have isolated a virus, which causes a mortal disease in cultured ornamental Koi and Common car... more We have isolated a virus, which causes a mortal disease in cultured ornamental Koi and Common carps (Cyprinus carpio) in many countries worldwide. This unclassified virus, which causes nephritis and gill necrosis, and so has been given the name carp nephritis and gill necrosis virus (CNGV), has a morphology resembling the herpes virus, but bears a genomic DNA of ca 250-300 kbp. So far, both others and we have been unable to find CNGV-DNA sequences possessing a significant similarity to known DNA viruses. The virus induces a lethal disease when water temperature ranges between 18 and 25 • C (permissive temperature). In this report, we demonstrate that carps, exposed to the virus at 23 • C for 3-5 days and then transferred to the non-permissive temperature of 30 • C, became resistant to a challenged infection and their sera demonstrated a high level of virus-specific antibodies. We have isolated attenuated non-pathogenic viruses that render virus-vaccinated carps resistant to the disease. Furthermore, vaccinated fish developed high levels of antibodies against the virus. We suggest, therefore, that this attenuated virus could be used as a live vaccine for the eradication of the mortal disease afflicting Common and ornamental carp fisheries in many countries.

Aquaculture Research, 2004

... Avner Cnaani 1 ,; Simon Tinman 2 ,; Yaacov Avidar 3 ,; Micha Ron 1 ,; Gideon Hulata 1. ... Th... more ... Avner Cnaani 1 ,; Simon Tinman 2 ,; Yaacov Avidar 3 ,; Micha Ron 1 ,; Gideon Hulata 1. ... The biochemical plasma components measured were total cholesterol (CHOL), triglyceride (TRIG), total protein (PROT), albumin (Alb), alkaline phosphatase (AP), aspartate aminotransferase ...

Journal of Clinical Microbiology, 2014

Tilapines are important for the sustainability of ecological systems and serve as the second most... more Tilapines are important for the sustainability of ecological systems and serve as the second most important group of farmed fish worldwide. Significant mortality of wild and cultured tilapia has been observed recently in Israel. The etiological agent of this disease, a novel RNA virus, is described here, and procedures allowing its isolation and detection are revealed. The virus, denominated tilapia lake virus (TiLV), was propagated in primary tilapia brain cells or in an E-11 cell line, and it induced a cytopathic effect at 5 to 10 days postinfection. Electron microscopy revealed enveloped icosahedral particles of 55 to 75 nm. Low-passage TiLV, injected intraperitoneally in tilapia, induced a disease resembling the natural disease, which typically presents with lethargy, ocular alterations, and skin erosions, with >80% mortality. Histological changes included congestion of the internal organs (kidneys and brain) with foci of gliosis and perivascular cuffing of lymphocytes in the...

Journal of Applied Ecology