András Saftics | Budapest University of Technology and Economics (original) (raw)

Papers by András Saftics

Research paper thumbnail of Cardiovesicles: A Liquid Biopsy Technology for Heart Failure and Cardiovascular Diseases

Journal of the American College of Cardiology, Apr 1, 2024

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Research paper thumbnail of Abstract 2081 Cancer Exosome Biogenesis Occurs Primarily by an Endocytosis-Independent Pathway

Journal of biological chemistry/˜The œJournal of biological chemistry, Mar 1, 2024

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Research paper thumbnail of Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of extracellular vesicles, Feb 1, 2024

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Research paper thumbnail of Molecular Atlas of HER2+ Breast Cancer Cells Treated with Endogenous Ligands: Temporal Insights into Mechanisms of Trastuzumab Resistance

Cancers, Jan 27, 2024

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Research paper thumbnail of Abstract P3167: Identification Of Macrophage-specific Markers On Extracellular Vesicles In A Transgenic Mouse Model And Humans

Circulation Research, Aug 3, 2023

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Research paper thumbnail of Exomap1 mouse: A transgenic model for in vivo studies of exosome biology

Extracellular vesicle, Nov 30, 2023

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Research paper thumbnail of Characterization of the dissolution of water microdroplets in oil

arXiv (Cornell University), Mar 27, 2020

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Research paper thumbnail of Single Extracellular VEsicle Nanoscopy

Journal of extracellular vesicles, Jul 1, 2023

Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations have ... more Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations have been defined not only by abundant tetraspanins (e.g., CD9, CD63 and CD81) but also by specific markers derived from their source cells. However, it remains a challenge to robustly isolate and characterize EV subpopulations. Here, we combined affinity isolation with super‐resolution imaging to comprehensively assess EV subpopulations from human plasma. Our Single Extracellular VEsicle Nanoscopy (SEVEN) assay successfully quantified the number of affinity‐isolated EVs, their size, shape, molecular tetraspanin content, and heterogeneity. The number of detected tetraspanin‐enriched EVs positively correlated with sample dilution in a 64‐fold range (for SEC‐enriched plasma) and a 50‐fold range (for crude plasma). Importantly, SEVEN robustly detected EVs from as little as ∼0.1 μL of crude plasma. We further characterized the size, shape and molecular tetraspanin content (with corresponding heterogeneities) for CD9‐, CD63‐ and CD81‐enriched EV subpopulations. Finally, we assessed EVs from the plasma of four pancreatic ductal adenocarcinoma patients with resectable disease. Compared to healthy plasma, CD9‐enriched EVs from patients were smaller while IGF1R‐enriched EVs from patients were larger, rounder and contained more tetraspanin molecules, suggestive of a unique pancreatic cancer‐enriched EV subpopulation. This study provides the method validation and demonstrates that SEVEN could be advanced into a platform for characterizing both disease‐associated and organ‐associated EV subpopulations.

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Research paper thumbnail of Exomap1 mouse: a transgenic model for<i>in vivo</i>studies of exosome biology

bioRxiv (Cold Spring Harbor Laboratory), May 29, 2023

ABSTRACTExosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the ... more ABSTRACTExosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the same topology as the cell, are enriched in selected exosome cargo proteins, and play important roles in health and disease. To address large unanswered questions regarding exosome biologyin vivo, we created theexomap1transgenic mouse model. In response to Cre recombinase,exomap1mice express HsCD81mNG, a fusion protein between human CD81, the most highly enriched exosome protein yet described, and the bright green fluorescent protein mNeonGreen. As expected, cell type-specific expression of Cre induced the cell type-specific expression of HsCD81mNG in diverse cell types, correctly localized HsCD81mNG to the plasma membrane, and selectively loaded HsCD81mNG into secreted vesicles that have the size (∼80 nm), topology (outside out), and content (presence of mouse exosome markers) of exosomes. Furthermore, mouse cells expressing HsCD81mNG released HsCD81mNG-marked exosomes into blood and other biofluids. Using high-resolution, single-exosome analysis by quantitative single molecule localization microscopy, we show here that that hepatocytes contribute ∼15% of the blood exosome population whereas neurons contribute &lt;1% of blood exosomes. These estimates of cell type-specific contributions to blood EV population are consistent with the porosity of liver sinusoidal endothelial cells to particles of ∼50-300 nm in diameter, as well as with the impermeability of blood-brain and blood-neuron barriers to particles &gt;5 nm in size. Taken together, these results establish theexomap1mouse as a useful tool forin vivostudies of exosome biology, and for mapping cell type-specific contributions to biofluid exosome populations. In addition, our data confirm that CD81 is a highly-specific marker for exosomes and is not enriched in the larger microvesicle class of EVs.

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Research paper thumbnail of Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Scientific Reports, Jan 9, 2020

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Research paper thumbnail of Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases

Scientific Reports, Feb 24, 2020

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Research paper thumbnail of Biológiai vékonyrétegek és élõ sejtek mechanikai tulajdonságainak vizsgálata kvarckristály mikromérleggel

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Research paper thumbnail of Oxidization increases the binding of EGCG to serum albumin revealed by kinetic data from label-free optical biosensor with reference channel

Analyst, 2020

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Research paper thumbnail of Bloch surface waves biosensing in the ultraviolet wavelength range – Bragg structure design for investigating protein adsorption by in situ Kretschmann-Raether ellipsometry

Applied Surface Science, 2021

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Research paper thumbnail of Biomimetic Dextran-Based Hydrogel Layers for Cell Micropatterning over Large Areas Using the FluidFM BOT Technology

Langmuir, Jan 17, 2019

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Research paper thumbnail of Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing

Biosensors, Jan 20, 2022

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Research paper thumbnail of Investigation of thin polymer layers for biosensor applications

Applied Surface Science, Sep 1, 2013

ABSTRACT Novel biosensors made of polymers may offer advantages over conventional technology such... more ABSTRACT Novel biosensors made of polymers may offer advantages over conventional technology such as possibility of mass production and tunability of the material properties. With the ongoing work on the polymer photonic chip fabrication in our project, simple model samples were tested parallel for future immobilization and accessing conditions for applications in typical aqueous buffers. The model samples consist of a thin, high refractive index polyimide film on top of TEOS on Si wafer. These model samples were measured by in situ spectroscopic ellipsometry using different aqueous buffers. The experiments revealed a high drift in aqueous solutions; the drift in the ellipsometric parameters (delta, psi) can be evaluated and presented as changes in thickness and refractive index of the polyimide layer. The first molecular layer of immobilization is based on polyethyleneimine (PEI). The signal for the PEI adsorption was detected on a stable baseline, only after a long conditioning. The stability of polyimide films in aqueous buffer solutions should be improved toward the real biosensor application. Preliminary results are shown on the possibilities to protect the polyimide. Optical Waveguide Lightmode Spectroscopy (OWLS) has been used to demonstrate the shielding effect of the thin TiO2 adlayer in biosensor applications.

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Research paper thumbnail of Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Scientific Reports, Dec 17, 2020

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Research paper thumbnail of Characterization of the Dissolution of Water Microdroplets in Oil

Colloids and interfaces, Feb 14, 2022

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Research paper thumbnail of Molecular Assessment of HER2 to Identify Signatures Associated with Therapy Response in HER2-Positive Breast Cancer

Cancers, Jun 4, 2022

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Research paper thumbnail of Cardiovesicles: A Liquid Biopsy Technology for Heart Failure and Cardiovascular Diseases

Journal of the American College of Cardiology, Apr 1, 2024

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Abstract 2081 Cancer Exosome Biogenesis Occurs Primarily by an Endocytosis-Independent Pathway

Journal of biological chemistry/˜The œJournal of biological chemistry, Mar 1, 2024

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of extracellular vesicles, Feb 1, 2024

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular Atlas of HER2+ Breast Cancer Cells Treated with Endogenous Ligands: Temporal Insights into Mechanisms of Trastuzumab Resistance

Cancers, Jan 27, 2024

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Abstract P3167: Identification Of Macrophage-specific Markers On Extracellular Vesicles In A Transgenic Mouse Model And Humans

Circulation Research, Aug 3, 2023

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Exomap1 mouse: A transgenic model for in vivo studies of exosome biology

Extracellular vesicle, Nov 30, 2023

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Characterization of the dissolution of water microdroplets in oil

arXiv (Cornell University), Mar 27, 2020

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Research paper thumbnail of Single Extracellular VEsicle Nanoscopy

Journal of extracellular vesicles, Jul 1, 2023

Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations have ... more Extracellular vesicles (EVs) and their cargo constitute novel biomarkers. EV subpopulations have been defined not only by abundant tetraspanins (e.g., CD9, CD63 and CD81) but also by specific markers derived from their source cells. However, it remains a challenge to robustly isolate and characterize EV subpopulations. Here, we combined affinity isolation with super‐resolution imaging to comprehensively assess EV subpopulations from human plasma. Our Single Extracellular VEsicle Nanoscopy (SEVEN) assay successfully quantified the number of affinity‐isolated EVs, their size, shape, molecular tetraspanin content, and heterogeneity. The number of detected tetraspanin‐enriched EVs positively correlated with sample dilution in a 64‐fold range (for SEC‐enriched plasma) and a 50‐fold range (for crude plasma). Importantly, SEVEN robustly detected EVs from as little as ∼0.1 μL of crude plasma. We further characterized the size, shape and molecular tetraspanin content (with corresponding heterogeneities) for CD9‐, CD63‐ and CD81‐enriched EV subpopulations. Finally, we assessed EVs from the plasma of four pancreatic ductal adenocarcinoma patients with resectable disease. Compared to healthy plasma, CD9‐enriched EVs from patients were smaller while IGF1R‐enriched EVs from patients were larger, rounder and contained more tetraspanin molecules, suggestive of a unique pancreatic cancer‐enriched EV subpopulation. This study provides the method validation and demonstrates that SEVEN could be advanced into a platform for characterizing both disease‐associated and organ‐associated EV subpopulations.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Exomap1 mouse: a transgenic model for<i>in vivo</i>studies of exosome biology

bioRxiv (Cold Spring Harbor Laboratory), May 29, 2023

ABSTRACTExosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the ... more ABSTRACTExosomes are small extracellular vesicles (sEVs) of ∼30-150 nm in diameter that have the same topology as the cell, are enriched in selected exosome cargo proteins, and play important roles in health and disease. To address large unanswered questions regarding exosome biologyin vivo, we created theexomap1transgenic mouse model. In response to Cre recombinase,exomap1mice express HsCD81mNG, a fusion protein between human CD81, the most highly enriched exosome protein yet described, and the bright green fluorescent protein mNeonGreen. As expected, cell type-specific expression of Cre induced the cell type-specific expression of HsCD81mNG in diverse cell types, correctly localized HsCD81mNG to the plasma membrane, and selectively loaded HsCD81mNG into secreted vesicles that have the size (∼80 nm), topology (outside out), and content (presence of mouse exosome markers) of exosomes. Furthermore, mouse cells expressing HsCD81mNG released HsCD81mNG-marked exosomes into blood and other biofluids. Using high-resolution, single-exosome analysis by quantitative single molecule localization microscopy, we show here that that hepatocytes contribute ∼15% of the blood exosome population whereas neurons contribute &lt;1% of blood exosomes. These estimates of cell type-specific contributions to blood EV population are consistent with the porosity of liver sinusoidal endothelial cells to particles of ∼50-300 nm in diameter, as well as with the impermeability of blood-brain and blood-neuron barriers to particles &gt;5 nm in size. Taken together, these results establish theexomap1mouse as a useful tool forin vivostudies of exosome biology, and for mapping cell type-specific contributions to biofluid exosome populations. In addition, our data confirm that CD81 is a highly-specific marker for exosomes and is not enriched in the larger microvesicle class of EVs.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy

Scientific Reports, Jan 9, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Human primary endothelial label-free biochip assay reveals unpredicted functions of plasma serine proteases

Scientific Reports, Feb 24, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Biológiai vékonyrétegek és élõ sejtek mechanikai tulajdonságainak vizsgálata kvarckristály mikromérleggel

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Oxidization increases the binding of EGCG to serum albumin revealed by kinetic data from label-free optical biosensor with reference channel

Analyst, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Bloch surface waves biosensing in the ultraviolet wavelength range – Bragg structure design for investigating protein adsorption by in situ Kretschmann-Raether ellipsometry

Applied Surface Science, 2021

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Biomimetic Dextran-Based Hydrogel Layers for Cell Micropatterning over Large Areas Using the FluidFM BOT Technology

Langmuir, Jan 17, 2019

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing

Biosensors, Jan 20, 2022

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Investigation of thin polymer layers for biosensor applications

Applied Surface Science, Sep 1, 2013

ABSTRACT Novel biosensors made of polymers may offer advantages over conventional technology such... more ABSTRACT Novel biosensors made of polymers may offer advantages over conventional technology such as possibility of mass production and tunability of the material properties. With the ongoing work on the polymer photonic chip fabrication in our project, simple model samples were tested parallel for future immobilization and accessing conditions for applications in typical aqueous buffers. The model samples consist of a thin, high refractive index polyimide film on top of TEOS on Si wafer. These model samples were measured by in situ spectroscopic ellipsometry using different aqueous buffers. The experiments revealed a high drift in aqueous solutions; the drift in the ellipsometric parameters (delta, psi) can be evaluated and presented as changes in thickness and refractive index of the polyimide layer. The first molecular layer of immobilization is based on polyethyleneimine (PEI). The signal for the PEI adsorption was detected on a stable baseline, only after a long conditioning. The stability of polyimide films in aqueous buffer solutions should be improved toward the real biosensor application. Preliminary results are shown on the possibilities to protect the polyimide. Optical Waveguide Lightmode Spectroscopy (OWLS) has been used to demonstrate the shielding effect of the thin TiO2 adlayer in biosensor applications.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Scientific Reports, Dec 17, 2020

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Characterization of the Dissolution of Water Microdroplets in Oil

Colloids and interfaces, Feb 14, 2022

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular Assessment of HER2 to Identify Signatures Associated with Therapy Response in HER2-Positive Breast Cancer

Cancers, Jun 4, 2022

Bookmarks Related papers MentionsView impact