gursimran singh | Bogomolets National Medical University (original) (raw)
Papers by gursimran singh
Molecular nutrition & food research, Jan 21, 2015
Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugat... more Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5 and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1 and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underw...
Journal of Medical Science And clinical Research
Introduction: Pleural effusion is an abnormal, collection of fluid in pleural space resulting fro... more Introduction: Pleural effusion is an abnormal, collection of fluid in pleural space resulting from excess of fluid production or decreased absorption. Exudates are produced by variety of inflammatory conditions and require more extensive evaluation and treatment than transudates. Laboratory testing (albumin, glucose, pH, LDH, cell counts) helps to reach the aetiology of pleural exudates. Present study was conducted to find out socio-clinical profile of plural effusion and to find out aetiology by using various laboratory tests. Aims and Objectives: To study socio-clinical profile and aetiology of pleural effusion. Material and Methods: Descriptive and Cross sectional study was conducted in Dept. of General Medicine, Dr. D.Y. Patil Medical College, Pune from Sept 2013 to Aug2015.Institute Ethics committee approvals obtained before the start of study. Total 50 cases were collected by convenient sampling method. Age more than 15years were included. Patients on AKT or ART, Age less than 15 years, pregnant women, post traumatic effusion and critically ill patients of pleural effusion on ventilator were excluded from study. Results: Maximum 29(58%) patients were in age group of 30-45 years, M:F= 2.3:1. Mean age was 37.34years and in range of 17-67years. More commonly presenting symptoms were pain in chest, fever and dyspnoea. Typical features of pleural effusion i.e. diminished movements, dull percussion note and absent breath sounds were observed in 40 (80%). Out of 50 cases 30 were exudative pleural effusion, and maximum 18 cases out of 30 were Tuberculosis as a cause for pleural effusion.
International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 2013
Molar pregnancy is characterized histologically by abnormalities of the chorionic villi that cons... more Molar pregnancy is characterized histologically by abnormalities of the chorionic villi that consist of trophoblastic proliferation and oedema of villous stroma. The incidence of hydatiform moles in India is 1 in 1000. The incidence of recurrence is 1 in 60 in a subsequent pregnancy and 1 in 65 in the third pregnancy after two molar pregnancies. The occurrence of hydatiform mole is related to a few risk factors such as smoking, increased paternal or maternal age, and previous history of abortions. Here we report a case of a 29 year old woman with history of four partial moles and two first trimester abortions and her management. The patient finally opted for adoption.
Molecular nutrition & food research, Jan 21, 2015
Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugat... more Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5 and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1 and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underw...
Journal of Medical Science And clinical Research
Introduction: Pleural effusion is an abnormal, collection of fluid in pleural space resulting fro... more Introduction: Pleural effusion is an abnormal, collection of fluid in pleural space resulting from excess of fluid production or decreased absorption. Exudates are produced by variety of inflammatory conditions and require more extensive evaluation and treatment than transudates. Laboratory testing (albumin, glucose, pH, LDH, cell counts) helps to reach the aetiology of pleural exudates. Present study was conducted to find out socio-clinical profile of plural effusion and to find out aetiology by using various laboratory tests. Aims and Objectives: To study socio-clinical profile and aetiology of pleural effusion. Material and Methods: Descriptive and Cross sectional study was conducted in Dept. of General Medicine, Dr. D.Y. Patil Medical College, Pune from Sept 2013 to Aug2015.Institute Ethics committee approvals obtained before the start of study. Total 50 cases were collected by convenient sampling method. Age more than 15years were included. Patients on AKT or ART, Age less than 15 years, pregnant women, post traumatic effusion and critically ill patients of pleural effusion on ventilator were excluded from study. Results: Maximum 29(58%) patients were in age group of 30-45 years, M:F= 2.3:1. Mean age was 37.34years and in range of 17-67years. More commonly presenting symptoms were pain in chest, fever and dyspnoea. Typical features of pleural effusion i.e. diminished movements, dull percussion note and absent breath sounds were observed in 40 (80%). Out of 50 cases 30 were exudative pleural effusion, and maximum 18 cases out of 30 were Tuberculosis as a cause for pleural effusion.
International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 2013
Molar pregnancy is characterized histologically by abnormalities of the chorionic villi that cons... more Molar pregnancy is characterized histologically by abnormalities of the chorionic villi that consist of trophoblastic proliferation and oedema of villous stroma. The incidence of hydatiform moles in India is 1 in 1000. The incidence of recurrence is 1 in 60 in a subsequent pregnancy and 1 in 65 in the third pregnancy after two molar pregnancies. The occurrence of hydatiform mole is related to a few risk factors such as smoking, increased paternal or maternal age, and previous history of abortions. Here we report a case of a 29 year old woman with history of four partial moles and two first trimester abortions and her management. The patient finally opted for adoption.