Silent infection of human dendritic cells by African and Asian strains of Zika virus. (original) (raw)

Vielle, Nathalie J.; Zumkehr, Beatrice; Garcia-Nicolas, Obdulio; Blank, Fabian; Stojanov, Miloš; Musso, Didier; Baud, David; Summerfield, Artur; Alves, Marco (2018). Silent infection of human dendritic cells by African and Asian strains of Zika virus. Scientific Reports, 8(1), p. 5440. Nature Publishing Group10.1038/s41598-018-23734-3

While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.

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Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Pneumologie (Erwachsene)04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Pneumology 05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
Graduate School:	Graduate School for Cellular and Biomedical Sciences (GCB)
UniBE Contributor:	Vielle, Nathalie Jane, Garcia Nicolas, Obdulio, Blank, Fabian, Summerfield, Artur, Alves, Marco
Subjects:	600 Technology > 610 Medicine & health 600 Technology > 630 Agriculture 500 Science > 570 Life sciences; biology
ISSN:	2045-2322
Publisher:	Nature Publishing Group
Language:	English
Submitter:	Nathalie Jane Vielle
Date Deposited:	18 Dec 2018 12:41
Last Modified:	05 Dec 2022 15:21
Publisher DOI:	10.1038/s41598-018-23734-3
PubMed ID:	29615676
Additional Information:	Article number: 5440
BORIS DOI:	10.7892/boris.121741
URI:	https://boris.unibe.ch/id/eprint/121741

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