Andreas Budi Wijaya | Brawijaya University (original) (raw)

Drafts by Andreas Budi Wijaya

Papers by Andreas Budi Wijaya

Research Journal of Pharmacy and Technology

Oxidative stress in β-TM (beta-thalassemia major) patients is associated with increased of malond... more Oxidative stress in β-TM (beta-thalassemia major) patients is associated with increased of malondialdehyde (MDA) level and also decreased of superoxide dismutase (SOD) level. Deferiprone and deferasirox, which are used for the treatment of iron overload, exhibit antioxidant potential. However, various clinical studies have shown an increase in creatinine levels in pediatric patients receiving oral iron chelator. There was limited study assessed oxidative stress and impact of β-TM on the renal function, especially in children that growing up with β-TM and receiving iron chelator. Therefore, the aim of the study is to investigate renal function and oxidative stress between β-TM patients at Ulin Hospital Banjarmasin who received deferasirox and deferiprone. Ninety β-TM patients (aged 2-≤18 years) with regular iron chelators (deferiprone or deferasirox) use at Ulin Hospital between October-December 2020, were included in this cross-sectional study. Laboratory examinations included compl...

Kanker leher rahim atau kanker serviks merupakan kanker pembunuh nomor dua di dunia setelah kanke... more Kanker leher rahim atau kanker serviks merupakan kanker pembunuh nomor dua di dunia setelah kanker payudara, namun merupakan kanker pembunuh nomor satu di Indonesia dengan persentase sekitar 25,91% dari seluruh kanker. Sifat immortal sel kanker terjadi karena adanya protein telomerase . Jika sebuah terapi mampu menonaktifkan telomerase pada sel kanker, maka akan terjadi pemendekan telomer seiring dengan pembelahan sel kanker yang kontinu. Berdasarkan hal di atas, antibodi terhadap telomerase berpotensi dikembangkan sebagai kandidat terapi kanker serviks untuk menghambat proliferasi sel kanker, yaitu melalui penghambatan aktivitas telomerase dan secara berkelanjutan akan mengobati kanker. Tujuan dari penelitian ini adalah untuk membuktikan bahwa antibodi telomerase dapat diproduksi sebagai agen terapi kanker leher rahim. Penelitian ini menggunakan desain eksperimen murni ( true experimental design ) dan dikerjakan secara in vivo dengan menggunakan hewan coba kelinci. Penelitian ini m...

Research Journal of Pharmacy and Technology, 2021

High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to P... more High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to Pseudomonas aeruginosa’s (P. aeruginosa) virulence and its ability to form biofilm. Biofilm’s formation is affected by the presence of rhamnolipid, whose production is regulated by quorum sensing systems. Piper betle (P. betle) possesses antimicrobial, antioxidant, anti-inflammatory and immunomodulatory properties. The aim of our study is to investigate the effects of P. betle leaf’s extract against biofilm formation and rhamnolipid production of P. aeruginosa. Active compounds of P. betle were identified using plate chromatography. Agar dilution method was used to determine the minimum biofilm inhibitory concentration (MBIC) of methanolic leaf extract of P. betle. A biofilm-producing P. aeruginosa isolate in the polystyrene plate adherence test was selected for confirmation of biofilm production by Scanning Electron Microscopy (SEM), after P. betle administration. Rhamnolipid detection a...

Cervical cancer is the first leading cause of cancer death in Indonesia. About 95% telomerase act... more Cervical cancer is the first leading cause of cancer death in Indonesia. About 95% telomerase activity is associated with cervical cancer’s malignancy. Therefore, telomerase is very potential as a biomarker for cervical cancer detection. This research’s purpose is to produce cut-off point of cervical cancer telomerase. Telomerase antibody is produced and detected using Western Blotting and ELISA, 15 samples of each cancer and normal patients were collected from RSSA. ELISA Absorbance value of antibody and antigen binding were collected and analyzed using independent t-test and ROC. cut off point is found at 0.092 with 81,25% specificity and 80% sensitivity. Keywords : Cervical Cancer, Telomerase, Telomerase Antibody, Cut-Off point

The Indian Journal of Pediatrics

Turkish Journal of Immunology, 2018

Introduction: Vitamin D is very important for Type 1 diabetes mellitus (T1 DM) pathogenesis, and ... more Introduction: Vitamin D is very important for Type 1 diabetes mellitus (T1 DM) pathogenesis, and plays major role in the regulation of pancreatic beta cells. However, serum vitamin D (25(OH)D 3) level related to inflammatory condition in T1 DM has not been sufficiently investigated. Therefore, we aimed to investigate the relationship between serum vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 levels in children with T1 DM. Material and Methods: Blood samples from 20 subjects of T1 DM patients and healthy patients were collected, and analyzed. Serum level of vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 between two groups were measured by using Indirect ELISA. Results: Serum vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 level between both groups were significantly different (p<0.001). It was shown that serum vitamin D (25(OH)D 3) and C-Peptide level had significant positive correlation, while serum C-Peptide and Interleukin-2 level had significant negative correlation (p=0.002; r=-0.658). Serum vitamin D (25(OH)D 3) and Interleukin-2 had also significant negative correlation (p<0.001; r=-0.753). Discussion: Serum vitamin D (25(OH)D 3) level is related to inflammatory condition in T1 DM. Low level of serum vitamin D (25(OH)D 3) in T1 DM patient tends to increase the level of Inteleukin-2, and it is highly correlated with pancreatic beta cells' impairment, which is marked by low C-Peptide level and high Inteleukin-2 level.

Journal of Hypertension

Background: Hypertension becomes the strongest risk factor for cardiovascular events, such stroke... more Background: Hypertension becomes the strongest risk factor for cardiovascular events, such stroke and acute coronary syndrome (ACS), as seen in RSUD Labuha. Blood pressure variability, especially Morning Blood Pressure Surge (MBPS) phase, might influence the onset time of cardiovascular events. Hypertensive mouse model shows Ccl19 overexpression, which responsible for exhibiting similar MBPS as seen in human essential hypertension. We aimed to investigate the relationship between cardiovascular events in RSUD Labuha and its onset time, which might related to MBPS. Human and mouse Ccl19 were also compared bioinformatically to elucidate probable involved mechanisms in human essential hypertension. Method: Observational cross-sectional study was held on 131 reports at Emergency Department of RSUD Labuha in 2016. Cardiovascular event was classified into three group based on its onset time; Morning (04.00–10.00), Noon (10.00–19.00), and Night (19.00–04.00). Data was analyzed using Chi-Square. Bioinformatical analysis was performed using Pymol, BLAT-UCSC Genome Browser, and EMBL-Pairwise Sequence Alignment. Result: 92 of 131 patients experiencing cardiovascular event were analyzed based on its onset time. Samples from this study were 46.6% male and 53.4% female. 31.3% patients were diagnosed with stroke, 38.9% with ACS and 69.5% with other cardiovascular events. While 22.2% patients had diabetes, 96.2% had hypertension, 47.3% had history of smoking, 9.2% experience kidney failure and 16.8% had dyslipidemia. Most events (54 (41.2%) subjects) were occurring in the morning, followed by noon onset in 21 (16.0%) subjects and night in 17 (13.0%) subjects. However, there were no significant differences between cardiovascular events and its onset time (p = 0.294). Conclusions: MBPS might increase risk for cardiovascular events occur in the morning in RSUD Labuha, but statistically not significant. Exaggerated MBPS in hypertensive mouse was related to high level of Ccl19, in association with Ccl19 gene polymorphism (SNP). Mouse Ccl19 (AA22–63) has 77.8% similarity, in comparison with human Ccl19 (AA356–1127).

BACKGROUND: Piper betle (P. betle) is widely used as a traditional medicine in Indonesia, with an... more BACKGROUND: Piper betle (P. betle) is widely used as a traditional medicine in Indonesia, with anti-quorum sensing and anti-biofilm activity. We investigated the impact of methanolic leaf extract of P. betle against Pseudomonas aeruginosa’s (P. aeruginosa) virulence factor, which associated with rhamnolipid (rhl) genes, METHODS: Minimum biofilm inhibitory concentration of the extract was determined at a concentration of 0.4% by agar dilution assay. The expression of rhlA and rhlC gene was assessed by using real-time polymerase chain reaction. RESULTS: All P. aeruginosa isolates contained rhlA, rhlB, and rhlC genes, which associated with rhl production. The expression of the rhlC gene decreased after administration of P. betle leaf extract at concentration of 0.4%, with beta coefficient was 0.662 (p = 0.019). CONCLUSION: The methanolic leaf extract of P. betle shows inhibition of rhlC gene expression, indicating the antirhl properties of P. betle against P. aeruginosa infection. Edit...

To the Editor: Novel coronavirus infection in Indonesia reached 140,000 cases by 17th August 2020... more To the Editor: Novel coronavirus infection in Indonesia reached 140,000 cases by 17th August 2020. There was inadequate data that supported the involvement of coagulation problems in pediatric cases, especially those with transfusion-dependent-thalassemia. We presented 4 pediatric cases of COVID-19 (positive oropharyngeal swab) and nonsplenectomized transfusiondependent-thalassemia (beta-thalassemia), which came for blood transfusion visit. Three patients were male and 1 patient was female. The age ranged from 9 y 8-mo-old to 17-y-old. All of our patients were mildly ill; mild respiratory symptoms were reported only in 2 cases; however, three patients showed lung infiltrate in the radiologic examination. None of them presented lymphopenia, thrombocytopenia, elevated CRP, elevated Troponin-T, or bleeding manifestation. The mean hemoglobin level was 6.7 g/dL. Elevated D-dimer was detected in 1 patient. Three thalassemic patients had prolongation of APTT or PT. None of our patients dev...

TURKISH JOURNAL OF MEDICAL SCIENCES, 2017

Background/aim: Telomerase activity is influenced by hTERT transcriptional regulation, shelterin,... more Background/aim: Telomerase activity is influenced by hTERT transcriptional regulation, shelterin, and posttranscriptional alternative splicing. Telomerase shelterin such as POT1 is highly correlated with various cancers. However, the profile of POT1 in cervical cancer has not been clearly identified. Therefore, it is important to identify its profile in cervical cancer biopsy tissue and normal cervical smears. Materials and methods: Biopsy tissue of cervical cancer patients and normal cervical smears were characterized using SDS-PAGE and western blot. Sixteen biopsy tissues of cervical cancer patients and 15 normal cervical smears were measured for POT1 level using ELISA. Results: The inline band at 70 kDa indicated that all samples had protein that was identified as POT1. Western blot showed that telomerase antibody only recognized POT1 in biopsy tissue of cervical cancer patients. There was a significant difference (P = 0.01) in POT1 level between biopsy tissue of cervical cancer patients and normal cervical smears. Conclusion: POT1 was identified at 70 kDa in biopsy tissue of cervical cancer patients and its level was higher than that in normal cervical smears. The high level of POT1 in the biopsy tissue of cervical cancer patients showed the influence of this shelterin component in cervical carcinogenesis and also cell immortalization.

Berkala Kedokteran

Subacute Sclerosing Panencephalitis (SSPE) is a progressive neurodegenerative disease that attack... more Subacute Sclerosing Panencephalitis (SSPE) is a progressive neurodegenerative disease that attacks the central nervous system, especially in the population of children and early adolescents, due to persistent measles virus infection. The incidence of SSPE is quite rare, and data shows that in developing countries it is still quite high compared to developed countries. Diagnosis is based on clinical, supporting examinations such as EEG, as well as increased antibodies against measles virus in serum and cerebrospinal fluid. Symptoms can include changes in behavior, myoclonus, memory problems, and persistent pyramidal or extrapyramidal movements. Management to date has not provided satisfactory results and is individualized. Most SSPE patients experience a progressive and gradual course, leading to death within 1-3 years. The challenge of SSPE in children is the approach to diagnosis and management. Until now, the approach to treating SSPE in children is still based on the pathophysiological mechanisms from several existing research studies. That why therapy guidelines for children with SSPE are still varied. This paper aims to discuss the topic of SSPE in children with a major focus on diagnostic and therapeutic approaches based on the latest scientific evidence.

Research Journal of Pharmacy and Technology

Oxidative stress in β-TM (beta-thalassemia major) patients is associated with increased of malond... more Oxidative stress in β-TM (beta-thalassemia major) patients is associated with increased of malondialdehyde (MDA) level and also decreased of superoxide dismutase (SOD) level. Deferiprone and deferasirox, which are used for the treatment of iron overload, exhibit antioxidant potential. However, various clinical studies have shown an increase in creatinine levels in pediatric patients receiving oral iron chelator. There was limited study assessed oxidative stress and impact of β-TM on the renal function, especially in children that growing up with β-TM and receiving iron chelator. Therefore, the aim of the study is to investigate renal function and oxidative stress between β-TM patients at Ulin Hospital Banjarmasin who received deferasirox and deferiprone. Ninety β-TM patients (aged 2-≤18 years) with regular iron chelators (deferiprone or deferasirox) use at Ulin Hospital between October-December 2020, were included in this cross-sectional study. Laboratory examinations included compl...

Kanker leher rahim atau kanker serviks merupakan kanker pembunuh nomor dua di dunia setelah kanke... more Kanker leher rahim atau kanker serviks merupakan kanker pembunuh nomor dua di dunia setelah kanker payudara, namun merupakan kanker pembunuh nomor satu di Indonesia dengan persentase sekitar 25,91% dari seluruh kanker. Sifat immortal sel kanker terjadi karena adanya protein telomerase . Jika sebuah terapi mampu menonaktifkan telomerase pada sel kanker, maka akan terjadi pemendekan telomer seiring dengan pembelahan sel kanker yang kontinu. Berdasarkan hal di atas, antibodi terhadap telomerase berpotensi dikembangkan sebagai kandidat terapi kanker serviks untuk menghambat proliferasi sel kanker, yaitu melalui penghambatan aktivitas telomerase dan secara berkelanjutan akan mengobati kanker. Tujuan dari penelitian ini adalah untuk membuktikan bahwa antibodi telomerase dapat diproduksi sebagai agen terapi kanker leher rahim. Penelitian ini menggunakan desain eksperimen murni ( true experimental design ) dan dikerjakan secara in vivo dengan menggunakan hewan coba kelinci. Penelitian ini m...

Research Journal of Pharmacy and Technology, 2021

High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to P... more High mortality rate and antimicrobial resistance are still becoming world-wide problems, due to Pseudomonas aeruginosa’s (P. aeruginosa) virulence and its ability to form biofilm. Biofilm’s formation is affected by the presence of rhamnolipid, whose production is regulated by quorum sensing systems. Piper betle (P. betle) possesses antimicrobial, antioxidant, anti-inflammatory and immunomodulatory properties. The aim of our study is to investigate the effects of P. betle leaf’s extract against biofilm formation and rhamnolipid production of P. aeruginosa. Active compounds of P. betle were identified using plate chromatography. Agar dilution method was used to determine the minimum biofilm inhibitory concentration (MBIC) of methanolic leaf extract of P. betle. A biofilm-producing P. aeruginosa isolate in the polystyrene plate adherence test was selected for confirmation of biofilm production by Scanning Electron Microscopy (SEM), after P. betle administration. Rhamnolipid detection a...

Cervical cancer is the first leading cause of cancer death in Indonesia. About 95% telomerase act... more Cervical cancer is the first leading cause of cancer death in Indonesia. About 95% telomerase activity is associated with cervical cancer’s malignancy. Therefore, telomerase is very potential as a biomarker for cervical cancer detection. This research’s purpose is to produce cut-off point of cervical cancer telomerase. Telomerase antibody is produced and detected using Western Blotting and ELISA, 15 samples of each cancer and normal patients were collected from RSSA. ELISA Absorbance value of antibody and antigen binding were collected and analyzed using independent t-test and ROC. cut off point is found at 0.092 with 81,25% specificity and 80% sensitivity. Keywords : Cervical Cancer, Telomerase, Telomerase Antibody, Cut-Off point

The Indian Journal of Pediatrics

Turkish Journal of Immunology, 2018

Introduction: Vitamin D is very important for Type 1 diabetes mellitus (T1 DM) pathogenesis, and ... more Introduction: Vitamin D is very important for Type 1 diabetes mellitus (T1 DM) pathogenesis, and plays major role in the regulation of pancreatic beta cells. However, serum vitamin D (25(OH)D 3) level related to inflammatory condition in T1 DM has not been sufficiently investigated. Therefore, we aimed to investigate the relationship between serum vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 levels in children with T1 DM. Material and Methods: Blood samples from 20 subjects of T1 DM patients and healthy patients were collected, and analyzed. Serum level of vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 between two groups were measured by using Indirect ELISA. Results: Serum vitamin D (25(OH)D 3), C-Peptide, and Interleukin-2 level between both groups were significantly different (p<0.001). It was shown that serum vitamin D (25(OH)D 3) and C-Peptide level had significant positive correlation, while serum C-Peptide and Interleukin-2 level had significant negative correlation (p=0.002; r=-0.658). Serum vitamin D (25(OH)D 3) and Interleukin-2 had also significant negative correlation (p<0.001; r=-0.753). Discussion: Serum vitamin D (25(OH)D 3) level is related to inflammatory condition in T1 DM. Low level of serum vitamin D (25(OH)D 3) in T1 DM patient tends to increase the level of Inteleukin-2, and it is highly correlated with pancreatic beta cells' impairment, which is marked by low C-Peptide level and high Inteleukin-2 level.

Journal of Hypertension

Background: Hypertension becomes the strongest risk factor for cardiovascular events, such stroke... more Background: Hypertension becomes the strongest risk factor for cardiovascular events, such stroke and acute coronary syndrome (ACS), as seen in RSUD Labuha. Blood pressure variability, especially Morning Blood Pressure Surge (MBPS) phase, might influence the onset time of cardiovascular events. Hypertensive mouse model shows Ccl19 overexpression, which responsible for exhibiting similar MBPS as seen in human essential hypertension. We aimed to investigate the relationship between cardiovascular events in RSUD Labuha and its onset time, which might related to MBPS. Human and mouse Ccl19 were also compared bioinformatically to elucidate probable involved mechanisms in human essential hypertension. Method: Observational cross-sectional study was held on 131 reports at Emergency Department of RSUD Labuha in 2016. Cardiovascular event was classified into three group based on its onset time; Morning (04.00–10.00), Noon (10.00–19.00), and Night (19.00–04.00). Data was analyzed using Chi-Square. Bioinformatical analysis was performed using Pymol, BLAT-UCSC Genome Browser, and EMBL-Pairwise Sequence Alignment. Result: 92 of 131 patients experiencing cardiovascular event were analyzed based on its onset time. Samples from this study were 46.6% male and 53.4% female. 31.3% patients were diagnosed with stroke, 38.9% with ACS and 69.5% with other cardiovascular events. While 22.2% patients had diabetes, 96.2% had hypertension, 47.3% had history of smoking, 9.2% experience kidney failure and 16.8% had dyslipidemia. Most events (54 (41.2%) subjects) were occurring in the morning, followed by noon onset in 21 (16.0%) subjects and night in 17 (13.0%) subjects. However, there were no significant differences between cardiovascular events and its onset time (p = 0.294). Conclusions: MBPS might increase risk for cardiovascular events occur in the morning in RSUD Labuha, but statistically not significant. Exaggerated MBPS in hypertensive mouse was related to high level of Ccl19, in association with Ccl19 gene polymorphism (SNP). Mouse Ccl19 (AA22–63) has 77.8% similarity, in comparison with human Ccl19 (AA356–1127).

BACKGROUND: Piper betle (P. betle) is widely used as a traditional medicine in Indonesia, with an... more BACKGROUND: Piper betle (P. betle) is widely used as a traditional medicine in Indonesia, with anti-quorum sensing and anti-biofilm activity. We investigated the impact of methanolic leaf extract of P. betle against Pseudomonas aeruginosa’s (P. aeruginosa) virulence factor, which associated with rhamnolipid (rhl) genes, METHODS: Minimum biofilm inhibitory concentration of the extract was determined at a concentration of 0.4% by agar dilution assay. The expression of rhlA and rhlC gene was assessed by using real-time polymerase chain reaction. RESULTS: All P. aeruginosa isolates contained rhlA, rhlB, and rhlC genes, which associated with rhl production. The expression of the rhlC gene decreased after administration of P. betle leaf extract at concentration of 0.4%, with beta coefficient was 0.662 (p = 0.019). CONCLUSION: The methanolic leaf extract of P. betle shows inhibition of rhlC gene expression, indicating the antirhl properties of P. betle against P. aeruginosa infection. Edit...

To the Editor: Novel coronavirus infection in Indonesia reached 140,000 cases by 17th August 2020... more To the Editor: Novel coronavirus infection in Indonesia reached 140,000 cases by 17th August 2020. There was inadequate data that supported the involvement of coagulation problems in pediatric cases, especially those with transfusion-dependent-thalassemia. We presented 4 pediatric cases of COVID-19 (positive oropharyngeal swab) and nonsplenectomized transfusiondependent-thalassemia (beta-thalassemia), which came for blood transfusion visit. Three patients were male and 1 patient was female. The age ranged from 9 y 8-mo-old to 17-y-old. All of our patients were mildly ill; mild respiratory symptoms were reported only in 2 cases; however, three patients showed lung infiltrate in the radiologic examination. None of them presented lymphopenia, thrombocytopenia, elevated CRP, elevated Troponin-T, or bleeding manifestation. The mean hemoglobin level was 6.7 g/dL. Elevated D-dimer was detected in 1 patient. Three thalassemic patients had prolongation of APTT or PT. None of our patients dev...

TURKISH JOURNAL OF MEDICAL SCIENCES, 2017

Background/aim: Telomerase activity is influenced by hTERT transcriptional regulation, shelterin,... more Background/aim: Telomerase activity is influenced by hTERT transcriptional regulation, shelterin, and posttranscriptional alternative splicing. Telomerase shelterin such as POT1 is highly correlated with various cancers. However, the profile of POT1 in cervical cancer has not been clearly identified. Therefore, it is important to identify its profile in cervical cancer biopsy tissue and normal cervical smears. Materials and methods: Biopsy tissue of cervical cancer patients and normal cervical smears were characterized using SDS-PAGE and western blot. Sixteen biopsy tissues of cervical cancer patients and 15 normal cervical smears were measured for POT1 level using ELISA. Results: The inline band at 70 kDa indicated that all samples had protein that was identified as POT1. Western blot showed that telomerase antibody only recognized POT1 in biopsy tissue of cervical cancer patients. There was a significant difference (P = 0.01) in POT1 level between biopsy tissue of cervical cancer patients and normal cervical smears. Conclusion: POT1 was identified at 70 kDa in biopsy tissue of cervical cancer patients and its level was higher than that in normal cervical smears. The high level of POT1 in the biopsy tissue of cervical cancer patients showed the influence of this shelterin component in cervical carcinogenesis and also cell immortalization.

Berkala Kedokteran

Subacute Sclerosing Panencephalitis (SSPE) is a progressive neurodegenerative disease that attack... more Subacute Sclerosing Panencephalitis (SSPE) is a progressive neurodegenerative disease that attacks the central nervous system, especially in the population of children and early adolescents, due to persistent measles virus infection. The incidence of SSPE is quite rare, and data shows that in developing countries it is still quite high compared to developed countries. Diagnosis is based on clinical, supporting examinations such as EEG, as well as increased antibodies against measles virus in serum and cerebrospinal fluid. Symptoms can include changes in behavior, myoclonus, memory problems, and persistent pyramidal or extrapyramidal movements. Management to date has not provided satisfactory results and is individualized. Most SSPE patients experience a progressive and gradual course, leading to death within 1-3 years. The challenge of SSPE in children is the approach to diagnosis and management. Until now, the approach to treating SSPE in children is still based on the pathophysiological mechanisms from several existing research studies. That why therapy guidelines for children with SSPE are still varied. This paper aims to discuss the topic of SSPE in children with a major focus on diagnostic and therapeutic approaches based on the latest scientific evidence.