Anupama S. kariyappa | Bangalore University (original) (raw)
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Papers by Anupama S. kariyappa
Biotechnological Utilization of Mangrove Resources, 2020
Abstract Mangroves are marine environments confined to temperate and subtropical intertidal zones... more Abstract Mangroves are marine environments confined to temperate and subtropical intertidal zones. They are rich sources of potent secondary metabolites due to their exposure to diverse marine habitats and environmental conditions. From time immemorial, they have been used in traditional medicine for their antioxidant and numerous pharmacological properties. However, the neuroprotective potential of these mangrove resources is not well explored. Therefore, in this chapter, we attempt to put forth information regarding the studies pertaining to the neuroprotective role of the mangroves and their bioactive compounds. The chapter will help to create awareness among researchers and clinicians to further investigate promising therapeutic candidate molecules from these potential mangrove plants in the treatment of neurodegenerative diseases.
International Journal of Nanomedicine, 2021
Purpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decale... more Purpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential. Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging ability were performed to assess the antioxidant potential of the fabricated NPs. Results: The bioactive component, 4-HIA, was efficiently encapsulated by the PLGA polymer and was found to be spherical and smooth with a size <10nm. 4-HIA showed better scavenging capability in DPPH and superoxide assays as compared to 4-HIA encapsulated PLGA and butylated hydroxytoluene (BHT). In contrast, 4-HIA encapsulated PLGA NPs exhibited a significant hydroxyl radical scavenging activity than 4-HIA and BHT alone. Further, the encapsulated NPs efficiently curtailed hydrogen peroxide (H 2 O 2)-induced cytotoxicity in PC12 cells. Conclusion: Our findings indicate that 4-HIA encapsulated PLGA NPs might be a therapeutic intervention towards the effective management of oxidative stress as it has exhibited efficient neuroprotective potential against H 2 O 2-induced oxidative stress in PC12 cells.
Critical Reviews™ in Oncogenesis, 2020
Hepatocellular carcinoma (HCC) stands third among cancer-related deaths globally. Owing to its hi... more Hepatocellular carcinoma (HCC) stands third among cancer-related deaths globally. Owing to its high incidence and linked mortality, early diagnosis is alarming for patient survival and the management of patients with developing HCC requires immediate attention. Advances in the knowledge of cancer biology and recognizing unique molecular candidates, including genomic and proteomic profiles, substantiate our understanding about several biological signatures connected with HCC. Precise identification and differential diagnosis of early HCC can remarkably improve patient survival. Currently, detection of HCC in clinical practice is performed by diagnostic imaging and noninvasive methods such as evaluation of serum biomarkers, growth factors, and the like. In this review, we discuss recent developments in targeting biomarkers for HCC.
Pharmacognosy Magazine, 2020
Background: In the current investigation, we explored the impact of swimming training and dietary... more Background: In the current investigation, we explored the impact of swimming training and dietary fortification of Hemidesmus indicus (HI) extract on markers of oxidative stress (OS) and antioxidant capacity in the middle-aged Wistar rats. Materials and Methods: Male rats received oral supplementation of HI at a dosage of 50 and 100 mg/kg B.W and swim trained for 30 min/day with 3% intensity, 6 days/week for a duration of 84 days. Results: Training and dietary intake of 100 mg/Kg B.W HI extract enhanced antioxidant enzyme activities in the discrete regions of the brain. The OS indicators were attenuated in response to single or combined interventions. The levels of thiols were significantly upregulated in SW-T (+HI2) group. Conclusion: Our findings suggest that both interventions improve antioxidant status, by attenuating the OS markers, which can be used as a therapeutic strategy in preventing age-associated neurological disorders.
Pharmacognosy Magazine, 2019
Background: Decalepis hamiltonii (Dh), a climbing shrub belonging to the family Apocynaceae, is c... more Background: Decalepis hamiltonii (Dh), a climbing shrub belonging to the family Apocynaceae, is consumed for its health-promoting properties. Objective: The present study was designed to evaluate the cardioprotective potential of Dh extract and its bioactive compounds on region-specific responses against isoproterenol (ISO)-induced myocardial injury in male Wistar rats. Materials and Methods: Rats were orally supplemented with ellagic acid (50 mg/kg body weight [BW]), 4-hydroxy isophthalic acid (30 mg/kg BW), and Dh extract (100 mg/kg BW) for 30 days, and they were subsequently administered (intraperitoneally) with ISO (150 mg/kg BW) for the last 2 days. Results: ISO-treated rats showed a significant increase in serum lipid profile, markers of cardiac damage, and decreased antioxidant status. Supplemented groups showed improved lipid profile, reduced serum marker enzymes, and enhanced antioxidant status in ISO-administered rats. Histopathological studies further confirmed the protective effect of Dh extract against ISO-induced myocardial infarction (MI). Conclusion: Our results demonstrated that Dh extract and its compounds efficiently ameliorated ISO-induced MI in rats.
National Journal of Physiology, Pharmacy and Pharmacology, 2019
Background: The progressive decline in learning and memory is an inevitable effect of aging. Nume... more Background: The progressive decline in learning and memory is an inevitable effect of aging. Numerous experimental evidences suggest that diet and physical exercise prevent cognitive deficits with age. Aims and Objectives: The aim of this study was designed to explore the influence of dietary supplementation of Hemidesmus indicus (HI) extract and swimming exercise on cognitive ability and brain-derived neurotrophic factor (BDNF) expression in rats. Materials and Methods: Male Wistar rats received oral supplementation of HI extract (50 and 100 mg/kg BW) and swim trained for 84 days, 30 min/day, 6 days/week. The rats were subjected to behavioral studies by T-maze followed by western blotting for BDNF expression. Results: The synergistic intervention was found to be more effective in improving cognition in terms of acquisition and retention. Training and supplementation of 100 mg/kg BW HI extract enhanced BDNF expression in the different regions of the brain, cerebral cortex, hippocampus, and cerebellum regions of the brain. Conclusion: Our findings suggest that both interventions improve cognitive ability and promote neurogenesis and can be used as a therapeutic strategy in preventing age-associated neurological disorders.
Medicinal Plants - International Journal of Phytomedicines and Related Industries, 2019
Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to... more Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to its health-promoting properties. However, the cardioprotective effect of Dh against oxidative stress remains unclear. In the present study, we investigated the protective effect of Dh on H2O2 induced oxidative stress in H9c2 cells. Pre-treatment with Dh extract prior to H2O2 exposure significantly increased the cell viability as measured by MTT assay and decreased the levels of LDH release. Dh extract inhibited H2O2 induced apoptosis and enhanced the number of viable cells in comparison to H2O2 treated cells as revealed by Flow cytometry analysis, AO/EB and DAPI staining. Furthermore, the Dh extract attenuated the H2O2 induced oxidative stress by up-regulating the antioxidant enzymes, reducing oxidative stress markers and inhibiting ROS generation. These results demonstrate that Dh extract ameliorates H2O2 induced cytotoxicity and may be a promising therapeutic intervention in treating oxidative stress related cardiovascular diseases.
Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to... more Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to its health-promoting properties. However, the cardio protective effect of Dh against oxidative stress remains unclear. In the present study, we investigated the protective effect of Dh on H 2 O 2 induced oxidative stress in H9c2 cells. Pre-treatment with Dh extract prior to H 2 O 2 exposure significantly increased the cell viability as measured by MTT assay and decreased the levels of LDH release. Dh extract inhibited H 2 O 2 induced apoptosis and enhanced the number of viable cells in comparison to H 2 O 2 treated cells as revealed by Flow cytometry analysis, AO/EB and DAPI staining. Furthermore, the Dh extract attenuated the H 2 O 2 induced oxidative stress by up-regulating the antioxidant enzymes, reducing oxidative stress markers and inhibiting ROS generation. These results demonstrate that Dh extract ameliorates H 2 O 2 induced cytotoxicity and may be a promising therapeutic intervention in treating oxidative stress related cardiovascular diseases.
Biotechnological Utilization of Mangrove Resources, 2020
Abstract Mangroves are marine environments confined to temperate and subtropical intertidal zones... more Abstract Mangroves are marine environments confined to temperate and subtropical intertidal zones. They are rich sources of potent secondary metabolites due to their exposure to diverse marine habitats and environmental conditions. From time immemorial, they have been used in traditional medicine for their antioxidant and numerous pharmacological properties. However, the neuroprotective potential of these mangrove resources is not well explored. Therefore, in this chapter, we attempt to put forth information regarding the studies pertaining to the neuroprotective role of the mangroves and their bioactive compounds. The chapter will help to create awareness among researchers and clinicians to further investigate promising therapeutic candidate molecules from these potential mangrove plants in the treatment of neurodegenerative diseases.
International Journal of Nanomedicine, 2021
Purpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decale... more Purpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential. Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging ability were performed to assess the antioxidant potential of the fabricated NPs. Results: The bioactive component, 4-HIA, was efficiently encapsulated by the PLGA polymer and was found to be spherical and smooth with a size <10nm. 4-HIA showed better scavenging capability in DPPH and superoxide assays as compared to 4-HIA encapsulated PLGA and butylated hydroxytoluene (BHT). In contrast, 4-HIA encapsulated PLGA NPs exhibited a significant hydroxyl radical scavenging activity than 4-HIA and BHT alone. Further, the encapsulated NPs efficiently curtailed hydrogen peroxide (H 2 O 2)-induced cytotoxicity in PC12 cells. Conclusion: Our findings indicate that 4-HIA encapsulated PLGA NPs might be a therapeutic intervention towards the effective management of oxidative stress as it has exhibited efficient neuroprotective potential against H 2 O 2-induced oxidative stress in PC12 cells.
Critical Reviews™ in Oncogenesis, 2020
Hepatocellular carcinoma (HCC) stands third among cancer-related deaths globally. Owing to its hi... more Hepatocellular carcinoma (HCC) stands third among cancer-related deaths globally. Owing to its high incidence and linked mortality, early diagnosis is alarming for patient survival and the management of patients with developing HCC requires immediate attention. Advances in the knowledge of cancer biology and recognizing unique molecular candidates, including genomic and proteomic profiles, substantiate our understanding about several biological signatures connected with HCC. Precise identification and differential diagnosis of early HCC can remarkably improve patient survival. Currently, detection of HCC in clinical practice is performed by diagnostic imaging and noninvasive methods such as evaluation of serum biomarkers, growth factors, and the like. In this review, we discuss recent developments in targeting biomarkers for HCC.
Pharmacognosy Magazine, 2020
Background: In the current investigation, we explored the impact of swimming training and dietary... more Background: In the current investigation, we explored the impact of swimming training and dietary fortification of Hemidesmus indicus (HI) extract on markers of oxidative stress (OS) and antioxidant capacity in the middle-aged Wistar rats. Materials and Methods: Male rats received oral supplementation of HI at a dosage of 50 and 100 mg/kg B.W and swim trained for 30 min/day with 3% intensity, 6 days/week for a duration of 84 days. Results: Training and dietary intake of 100 mg/Kg B.W HI extract enhanced antioxidant enzyme activities in the discrete regions of the brain. The OS indicators were attenuated in response to single or combined interventions. The levels of thiols were significantly upregulated in SW-T (+HI2) group. Conclusion: Our findings suggest that both interventions improve antioxidant status, by attenuating the OS markers, which can be used as a therapeutic strategy in preventing age-associated neurological disorders.
Pharmacognosy Magazine, 2019
Background: Decalepis hamiltonii (Dh), a climbing shrub belonging to the family Apocynaceae, is c... more Background: Decalepis hamiltonii (Dh), a climbing shrub belonging to the family Apocynaceae, is consumed for its health-promoting properties. Objective: The present study was designed to evaluate the cardioprotective potential of Dh extract and its bioactive compounds on region-specific responses against isoproterenol (ISO)-induced myocardial injury in male Wistar rats. Materials and Methods: Rats were orally supplemented with ellagic acid (50 mg/kg body weight [BW]), 4-hydroxy isophthalic acid (30 mg/kg BW), and Dh extract (100 mg/kg BW) for 30 days, and they were subsequently administered (intraperitoneally) with ISO (150 mg/kg BW) for the last 2 days. Results: ISO-treated rats showed a significant increase in serum lipid profile, markers of cardiac damage, and decreased antioxidant status. Supplemented groups showed improved lipid profile, reduced serum marker enzymes, and enhanced antioxidant status in ISO-administered rats. Histopathological studies further confirmed the protective effect of Dh extract against ISO-induced myocardial infarction (MI). Conclusion: Our results demonstrated that Dh extract and its compounds efficiently ameliorated ISO-induced MI in rats.
National Journal of Physiology, Pharmacy and Pharmacology, 2019
Background: The progressive decline in learning and memory is an inevitable effect of aging. Nume... more Background: The progressive decline in learning and memory is an inevitable effect of aging. Numerous experimental evidences suggest that diet and physical exercise prevent cognitive deficits with age. Aims and Objectives: The aim of this study was designed to explore the influence of dietary supplementation of Hemidesmus indicus (HI) extract and swimming exercise on cognitive ability and brain-derived neurotrophic factor (BDNF) expression in rats. Materials and Methods: Male Wistar rats received oral supplementation of HI extract (50 and 100 mg/kg BW) and swim trained for 84 days, 30 min/day, 6 days/week. The rats were subjected to behavioral studies by T-maze followed by western blotting for BDNF expression. Results: The synergistic intervention was found to be more effective in improving cognition in terms of acquisition and retention. Training and supplementation of 100 mg/kg BW HI extract enhanced BDNF expression in the different regions of the brain, cerebral cortex, hippocampus, and cerebellum regions of the brain. Conclusion: Our findings suggest that both interventions improve cognitive ability and promote neurogenesis and can be used as a therapeutic strategy in preventing age-associated neurological disorders.
Medicinal Plants - International Journal of Phytomedicines and Related Industries, 2019
Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to... more Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to its health-promoting properties. However, the cardioprotective effect of Dh against oxidative stress remains unclear. In the present study, we investigated the protective effect of Dh on H2O2 induced oxidative stress in H9c2 cells. Pre-treatment with Dh extract prior to H2O2 exposure significantly increased the cell viability as measured by MTT assay and decreased the levels of LDH release. Dh extract inhibited H2O2 induced apoptosis and enhanced the number of viable cells in comparison to H2O2 treated cells as revealed by Flow cytometry analysis, AO/EB and DAPI staining. Furthermore, the Dh extract attenuated the H2O2 induced oxidative stress by up-regulating the antioxidant enzymes, reducing oxidative stress markers and inhibiting ROS generation. These results demonstrate that Dh extract ameliorates H2O2 induced cytotoxicity and may be a promising therapeutic intervention in treating oxidative stress related cardiovascular diseases.
Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to... more Decalepis hamiltonii (Dh) is used to treat various diseases in Indian traditional medicine due to its health-promoting properties. However, the cardio protective effect of Dh against oxidative stress remains unclear. In the present study, we investigated the protective effect of Dh on H 2 O 2 induced oxidative stress in H9c2 cells. Pre-treatment with Dh extract prior to H 2 O 2 exposure significantly increased the cell viability as measured by MTT assay and decreased the levels of LDH release. Dh extract inhibited H 2 O 2 induced apoptosis and enhanced the number of viable cells in comparison to H 2 O 2 treated cells as revealed by Flow cytometry analysis, AO/EB and DAPI staining. Furthermore, the Dh extract attenuated the H 2 O 2 induced oxidative stress by up-regulating the antioxidant enzymes, reducing oxidative stress markers and inhibiting ROS generation. These results demonstrate that Dh extract ameliorates H 2 O 2 induced cytotoxicity and may be a promising therapeutic intervention in treating oxidative stress related cardiovascular diseases.