Mahaboob basha P | Bangalore University (original) (raw)

Papers by Mahaboob basha P

Fluoride, 2009

Premated 3-month-old albino rats received 200-ppm fluoride ion (F) in their drinking water; the p... more Premated 3-month-old albino rats received 200-ppm fluoride ion (F) in their drinking water; the pups born to them were separately administered, in groups of six, daily doses of clinoptilolite, zinc, selenium, vitamin C, vitamin D, and propolis. On post-partum day 45, the pups were sacrificed, brain regions separated, and oxidative stress markers were analyzed. Prenatal (maternal) and postnatal F exposure in the developing rats caused a significant increase in the activity of lipid peroxidation and a decrease in catalase, superoxide dismutase, and glutathione peroxidase activity, thus indicating vulnerability of the developing brain to oxidative stress. Alterations were region specific, and oral supplementation of the listed antioxidants not only inhibited oxidative stress but also enhanced the activity of antioxidant enzymes. Administration of antioxidants during F exposure significantly overcame neuronal F toxicity (mostly with p<0.05 or <0.01) and therefore may be a therapeutic strategy for fluorotic victims.

International journal of innovative science and research technology, Jul 30, 2020

Gestational diabetes is unique because of the diversity of problems that can affect the embryo/fe... more Gestational diabetes is unique because of the diversity of problems that can affect the embryo/fetus beginning with conception. Streptozotocin (STZ), a diabetogenic agent when administered to pregnant rats in high dose, induces diabetes by destructing pancreatic β-islet cells resultantly in the intrauterine life of developing fetuses limits their adaptation with depleted insulin secretion. Similarly, environmental stressors like cold-stress result in fetal hypoinsulinemia with a reduction in the number of insulin receptors on target cells. In a given situation, if both stressors are prevailing, the resultant free radical production in prenatal life may bring severe oxidative stress on the molecular integrity of proteins that might progress to weaning and adulthood. In this study, the oxidative indices measured in STZ induced gestational rats upon exposure to cold stress (15°C & 20°C) indicate significant changes in discrete brain regions. Cold-stress found to exacerbate the free radical production in diabetic subjects and impose a higher rate of protein oxidation confirming synergetic effects. The findings for the first time confirm that the oxidative changes that occurred due to prenatal stress remain into weaning and adulthood, specifically in the functional areas like the cerebral cortex and hippocampus, which in turn may bring impairments/deficits in memory and cognitive processes.

International journal of innovative science and research technology, Jul 1, 2020

Ca 2+ sequestration and its homeostasis is disrupted when mitochondrial membrane permeabilises to... more Ca 2+ sequestration and its homeostasis is disrupted when mitochondrial membrane permeabilises to form a large opening-the mitochondrial permeability transition pore (MPTP). The MPTP formation is common during aging and age related pathologies, activating cell death pathways to avoid unhealthy consequences and malignancies in the brain tissue. Several studies have identified the participation of Cyclophilin-D (Cyp-D) and adenine nucleotide translocase(ANT) in forming MPTP. However, Ca 2+ is known to participate significantly in MPTP induction, although its concentration and time dependent permeabilization mechanisms are still elusive. In this work, we have focused on the contribution of Ca 2+ participation, its concentration and time taken to permeabilze the mitochondrial inner membrane as a measure of light scattering at 540 ηM. We have observed that MPTP formation is increased in mitochondria isolated from aged rats in comparison to young adult and neonatal rats. The cyclosporin A application blocks the cyclophilin interaction thus avoiding MPTP formation confirming the crucial role of Ca 2+ inducted MPTP opening. A 100 µM Ca 2+ incubation for 15 minutes allowed the 50% probability of formation of MPTP in mitochondria isolated from all age groups. Thus, alleviating its role in aging and neurodegeneration.

Mining, Metallurgy & Exploration, 2022

Biological Trace Element Research, 2016

Fluoride toxicity is known to pose infertility in fluoride-intoxicated animals as well as in peop... more Fluoride toxicity is known to pose infertility in fluoride-intoxicated animals as well as in people residing in fluoride endemic zones. The present study addresses the degree of impairments caused due to co-exposure of high fluoride toxicity in diabetic mice. Swiss mice, Mus musculus, were subjected to fluoride toxicity by providing fluoride-supplemented drinking water (600 ppm NaF) for a period of 30 days after the confirmation of streptozotocin-induced diabetes(STZ, 50 mg/kgbw). Consequently, aggravated hyperglycemia and tissue fluoride accumulation were witnessed in fluoride-intoxicated diabetic mice; later, these toxicated mice were treated with ginseng extract (GE) and banaba leaf extract, (BLE) at dose of 150 mg/kgbw/day alone and in combination for 15 and 30-day duration to check the efficacy of phytoextracts in reversing the toxicity. The spermatological indices studied, such as sperm density, motility, viability and morphology as well as the testicular biochemical parameters showed enhanced impairment in reproductive status of fluoride-intoxicated diabetic mice. Further, 15-days administration of GE and BLE in combination at a dose of 150 mg/kgbw/day was found to be beneficial in normalizing the alterations observed upon fluoride intoxication to diabetic mice. However, the correlates showed moderate association between blood glucose levels and the spermatological as well as biochemical indices wherein the tissue fluoride levels correlate least.

International Journal of Medical Science and Public Health

in Australia, the women who get pregnant during winter are at a higher risk due to gestational di... more in Australia, the women who get pregnant during winter are at a higher risk due to gestational diabetes. [2,3] Even in other parts of the world, the incidence has seen a definite rise in recent times. The prevalence rates are between 6% and 14% in East and West Africa [4] and between 13% and 18% in South Asia. In Indian population, the incidence of GDM is 14.42% which relies on Diabetes in Pregnancy Study Group India recommendation [5] while, its awareness and possible morbid outcomes among mothers are very low (6%). [5] Existing data suggest that the hypothalamicpituitary-adrenal axis (HPA) and its hormonal response through cortisol, are liable for critical neurodevelopmental deficits in the juvenile offspring which is transduced due to Research Article Background: Prenatal stress is unique due to range of problems and can affect the embryo/fetus beginning with conception. Gestational diabetes mellitus is the concern for expectant-mothers wherein glucose intolerance with consistent hyperglycemia is a threatening factor during pregnancy. Objectives: In the event of multiple stressors posing their effects on intrauterine life and placenta being the target of increased sympathetic tone during gestation, there is a possibility of functional vulnerabilities that may contribute to the pathogenesis in post-natal life. Studying brain regional discrepancies in offspring might help to know the prenatal stress-induced variation in the antioxidant barrier and promoted oxidative stress. Materials and Methods: The changes occurring in oxidative stress indices in discrete brain regions of rat offspring born as a consequential exposure to gestational diabetes (streptozotocin induction) and cold stress (15 and 20°C) are assessed in this study. Results: The findings specify the involvement of cold-stress provoked induction of higher degree oxidative stress within brain compartments as evidenced by a decrease in antioxidant enzymes, namely, superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, and GSH as well as increase in the concentration of malondialdehyde. Results highlight the synergistic actions of stressors due to the increased generation of free radicals. Cold stress at 15°C found to cause exacerbatory actions by depleting antioxidant enzymes in diabetic subjects than the exposures made at 20°C. Conclusion: The findings prove that cold stress is a crucial stimulus to a fetus during gestation and acts as a trigger of oxidative stress especially in diabetic subjects and can pose an adverse impact. These changes could partly explain the increased vulnerability of prenatally stressed subjects to functional disorders including deficits in memory and cognitive processes in later life.

Environmental science and pollution research international, Jan 12, 2016

With the limited but ongoing usage of di-n-butyl phthalate (DBP) as plasticizer, the health effec... more With the limited but ongoing usage of di-n-butyl phthalate (DBP) as plasticizer, the health effects of both phthalate and its alternatives are far from being understood. Multigenerational effects of phthalates were evaluated in rats upon exposure to DBP, aiming to provide some evidences about its potential in causing developmental teratogenicity. Gestational rats were exposed to DBP (500 mg/kg bw/day) and control groups with olive oil. On the 18th day of gestation, fetuses (F1) isolated from a few dams were subjected to prenatal screening, and the other rats were allowed to litter, and later postnatal screening was made. DBP-toxicated (F1) rats were crossed and reared up to three generations (F2 and F3) by adopting the same experimental design. A considerable decrease in the weight of placenta, low number of corpora lutea and increased resorptions, and pre- and postimplantation loss were observed in F1, F2, and F3 generations. Further, there was a decrease in the number of live birt...

Indian journal of experimental biology, 2011

Aqueous leaf extract of L. speciosa (banaba) effectively decreased the blood glucose in streptozo... more Aqueous leaf extract of L. speciosa (banaba) effectively decreased the blood glucose in streptozotocin-induced diabetic mice after 15th day of banaba exposure. Further, banaba leaf extract have the potential to inhibit lipid peroxidation and effectively intercept/neutralize reactive oxygen species such as super oxide, H2O2 and NO based free radicals. The aqueous banaba leaf extract (150 mg/kg bodyweight) duly reduced STZ generated reactive intermediates and radical species helping to regulate normal levels of antioxidative markers like superoxide dismutase, catalase, glutathione-S-transferase and reduced glutathione.

Journal of Pharmaceutical and Scientific Innovation, 2013

Neurochemical Research, 2010

The effect of fluoride exposure during gestation and post gestation periods were studied to check... more The effect of fluoride exposure during gestation and post gestation periods were studied to check the status of oxidant, antioxidant and macromolecular changes in CNS and ameliorative role of antioxidants. The pregnant Wistar albino rats were exposed to 50 and 150 ppm fluoride in drinking water and the pups born to them were used for experimentation. After postpartum, the pups were administered daily selected antioxidants through oral gavage. On 21st postnatal day pups were sacrificed and biochemical parameters were assessed. Fluoride exposure substantially increased the activity/levels of fluoride, LPO, protein oxidation, MAO-B, GOT, GPT and decreased protein thiols, RNA and total proteins in discrete regions of CNS. The findings evidenced fluoride induced dyshomeostasis caused on antioxidants, enzymes, macromolecules and governed the pathophysiological events leading to functional loss in a dose dependent manner. The administration of antioxidants remedied the disquiet caused by high fluoride exposure at extreme vulnerable periods of life.

Cellular and Molecular Neurobiology, 2013

Biologia, 2011

Fluoride has been implicated as a pathologic mediator of fluorosis. Interestingly neuronal destru... more Fluoride has been implicated as a pathologic mediator of fluorosis. Interestingly neuronal destruction, synaptic injury occurs by a mechanism involving oxidative stress, however, its effects in developmental stages of life, during maternal fluoride exposure and amelioration are not elucidated. In the present study, pregnant Wistar albino rats were exposed to 50 and 150 ppm fluoride in drinking water during gestation and post gestation. After parturition the pups born to the experimental animals were administered daily with selected antioxidants for 21 consecutive days. Fluoride administration substantially enhanced fluoride accumulation, lipid peroxidation and decreased the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione levels in discrete regions of central nervous system. The results significantly (P < 0.05) demonstrated the effect of fluoride through exacerbated oxidative damage and disrupted antioxidant homeostasis...

Toxicology Reports, 2020

The extensive use of din -butyl phthalate (DBP) as a plasticizer in medical devices, personal car... more The extensive use of din -butyl phthalate (DBP) as a plasticizer in medical devices, personal care products, and industries, which is a major threat to humankind as it leaches out easily from the plastic matrix into the environment. Health risks posed to adults and children from the broad usage of DBP in cosmetics and infant toys observed predominantly due to repeated and prolonged exposure. Hence, this study was undertaken to evaluate the potential effect of DBP in the hepatic tissue of rats up to three generations. Wistar rats were induced at a dose of 500 mg DBP /kg body weight dissolved in olive oil by oral gavage throughout gestation (GD 6-21), lactation and post-weaning and reared by crossing intoxicated rats up to three generations. Results of the present study showed a significant increase in the relative weight of liver, while decreased levels of antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathione (GSH) was evident in DBP treated rats at P < 0.05. Besides hepatic marker enzymes viz., alanine transaminase (ALT) and aspartate transaminase (AST) were elevated significantly in experimental rats compared to those of the control group. Furthermore, histological studies revealed congested central veins and dilated sinusoids in F 1 progeny while mild to severe focal inflammatory infiltrations were evident in F 2 & F 3 rats. Negative correlation observed between the levels of antioxidant enzymes and transaminase activity. In brief, DBP exposure elicits oxidative stress and alters the transaminase activity levels causing damage in hepatic tissue. F 3 progeny found to high vulnerability to the exposure of DBP than F 2 & F 1 rats.

Cellular and Molecular Neurobiology, 2014

Mitochondrial dysfunction and consequent energy depletion are the major causes of oxidative stres... more Mitochondrial dysfunction and consequent energy depletion are the major causes of oxidative stress resulting to bring alterations in the ionic homeostasis causing loss of cellular integrity. Our previous studies have shown the age-associated interactive effects in rat central nervous system (CNS) upon co-exposure to chlorpyrifos (CPF) and cold stress leading to macromolecular oxidative damage. The present study elucidates a possible mechanism by which CPF and cold stress interaction cause(s) mitochondrial dysfunction in an age-related manner. In this study, the activity levels of Krebs cycle enzymes and electron transport chain (ETC) protein complexes were assessed in the isolated fraction of mitochondria. CPF and cold stress (15 and 20°C) exposure either individually or in combination decreased the activity level of Krebs cycle enzymes and ETC protein complexes in discrete regions of rat CNS. The findings confirm that cold stress produces significant synergistic effect in CPF intoxicated aging rats. The synergism between CPF and cold stress at 15°C caused a higher depletion of respiratory enzymes in comparison with CPF and cold stress alone and together at 20°C indicating the extent of deleterious functional alterations in discrete regions of brain and spinal cord (SC) which may result in neurodegeneration and loss in neuronal metabolic control. Hence, co-exposure of CPF and cold stress is more dangerous than exposure of either alone. Among the discrete regions studied, the cerebellum and medulla oblongata appears to be the most susceptible regions when compared to cortex and SC. Furthermore, the study reveals a gradual decrease in sensitivity to CPF toxicity as the rat matures.

Safer antioxidants from plant origin are essential to prevent the progression of free radical med... more Safer antioxidants from plant origin are essential to prevent the progression of free radical mediated disorders. Current research is directed towards finding naturally-occurring antioxidants of plant origin that provided efficacy by additive or synergistic activities. Panax ginseng (ginseng) and Lagerstroemia speciosa (banaba) are suggested to have bioactive components having health benefits. The present study investigates on the free radical scavenging potential as well as total phenolic and flavanoid contents of aqueous extract of ginseng and banaba. They were analysed for total antioxidant activity by TEAC assay, superoxide, hydroxyl, hydrogen peroxide and nitric oxide radical scavenging activities as well as total phenolic and flavanoid contents. The calculated results with trolox standard curve, the TEAC value explain the antioxidant potential of the GE overhaul BLE. In superoxide and nitric oxide radical scavenging assays, both GE and BLE showed almost similar range of activi...

Di-n-butyl phthalate (DBP) is a well-known plasticizer and cause a wide range of reproductive or ... more Di-n-butyl phthalate (DBP) is a well-known plasticizer and cause a wide range of reproductive or endocrine disruptive disorders. Exposure to DBP during gestation shown to disrupt testosterone synthesis and male sexual development in the fetal rat, however, the underlying mechanisms and its impact on the cortical and cerebellar neuronal development remain poorly understood. Here, we examine the effect of multigenerational exposure of DBP on rat cortical as well as cerebellar neurons. In this study 1/16 LD50 (i.e., 500mg/kg BW) administered through oral gavage during gestation day 6 to till parturition; exposure continued until they were weaned on postnatal (PND) 30. All pups at PND30 were analysed for cytoarchitectural alternations in brain cortex and cerebellum. Variations were observed in the rate of feed and water consumption, litter size, organ (brain) somatic index and mortality. Significant degenerative changes in neuroglial cells of brain cortex of F1; focal aggregates of infl...

Indian journal of experimental biology, 1993

Sublethal doses of methyl parathion (o, o-dimethyl-o-nitrophenyl thiophosphate) injected intraper... more Sublethal doses of methyl parathion (o, o-dimethyl-o-nitrophenyl thiophosphate) injected intraperitoneally to 7th day old developing albino rat pups induced alterations in the inhibitory GABAergic system of CNS. A substantial simulation of the inhibitory system was noticed. A profound increase was found in the level of the inhibitory transmitter, GABA on methyl parathion injection. An increase in the activity levels of the enzymes glutamic acid decarboxylase and 4-aminobutyrate-2-oxoglutarate-amino transferase in the cortex, brain stem and spinal cord of the CNS was found. It is observed that methyl parathion causes potentiation of the inhibitory transmission (GABAergic system) in the wake of inducing suppression of cholinergic system in CNS of developing rat pups.

Indian journal of experimental biology, 2001

Methyl parathion induced alterations in the level of monoamines, viz. norepinephrine, dopamine an... more Methyl parathion induced alterations in the level of monoamines, viz. norepinephrine, dopamine and serotonin were studied in discrete regions of developing central nervous system of rat pups. A significant decrease in the level of monoamines noticed in methyl parathion toxicosis may be related to the altered neuronal activity and inefficiency, leading to depression and impairment in various behavioural activities. In contrast to AChE inhibition, monoamine oxidase (MAO) activity showed an increasing trend and it could cause deamination of catecholamines and accumulation of its metabolites. This suggests that an increased AChE inhibition may indirectly stimulate MAO activity in developing rat pups exposed to methyl parathion.