Stephen Koury | SUNY: University at Buffalo (original) (raw)
Papers by Stephen Koury
British Journal of Haematology, 1996
Contributions to nephrology
Seminars in nephrology, 1993
Contributions to nephrology, 1989
Seminars in hematology, 1991
Klinische Wochenschrift, 1991
The kidney is the main site of erythropoietin (EPO) formation. Oxygen sensing in the kidney itsel... more The kidney is the main site of erythropoietin (EPO) formation. Oxygen sensing in the kidney itself plays a major role in the control of EPO synthesis. By in situ hybridization it has been established that the EPO-producing cells are situated in the interstitium of the cortical labyrinth, but they have not been precisely identified. Morphological findings provide new insights into the location and mechanism of oxygen sensing in the kidney. In addition to causing an increase in the number of cells containing EPO messenger RNA, anemia provokes structural changes exclusively in the cortical labyrinth. Specifically, the fibroblasts become enlarged and show increased activity of 5'-nucleotidase, and the $1 segment of the proximal tubule shows similar alterations as in various models of hypoxia. Thus, structures that are situated in the close vicinity of the EPO-producing cells appear to be sensitive to decreased oxygen delivery.
Future Oncology, 2014
Breast cancer is one of the most prevalent cancers in women worldwide [1,2] and is the most commo... more Breast cancer is one of the most prevalent cancers in women worldwide [1,2] and is the most common cause of cancer deaths in women [2]. The deaths related to breast cancer are not due to the primary tumors, but are due to the metastases to the lungs, brain and bone [3,4]. Therefore, treatment and management to block metastases is an important target for therapy that would improve survival in breast cancer patients.
Applied Radiation and Isotopes, 2008
Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antige... more Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antigen (TF-Ag) mAb that bound both alpha-and beta-linked TF-Ag. In this report, 124 I-labeled mAb JAA-F11 specific for alpha-linked TF-Ag showed higher tumor specificity in in vivo micro-positron emission tomography (micro-PET) of the mouse mammary adenocarcinoma line, 4T1, showing no preferential uptake by the kidney. Labeled product remained localized in the tumor for at least 20 days. Glycan array analysis showed structural specificity of the antibody. r
Breast cancer is one of the most prevalent cancers in women worldwide [1, and is the most common ... more Breast cancer is one of the most prevalent cancers in women worldwide [1, and is the most common cause of cancer deaths in women . The deaths related to breast cancer are not due to the primary tumors, but are due to the metastases to the lungs, brain and bone . Therefore, treatment and management to block metastases is an important target for therapy that would improve survival in breast cancer patients.
Cell and tissue research, 1987
The Journal of cell biology, Jan 1, 1988
Spectrin-like proteins are found in a wide 9 The Rockefeller University Press,
Proceedings of the …, Jan 1, 1991
Synthesis of erythropoietin, the primary humoral regulator of erythropoiesis, in liver and kidney... more Synthesis of erythropoietin, the primary humoral regulator of erythropoiesis, in liver and kidney is inducible by anemia or hypoxia. Analysis of human erythropoietin gene expression in transgenic mice revealed that sequences located 6-14 kilobases 5' to the gene direct expression to the kidney, whereas sequences within the immediate 3'flanking region control hepatocyte-specific expression. Human erythropoietin transcription initiation sites were differentially utilized in liver and kidney. Inducible transgene expression was precisely targeted to peritubular interstitial cells in the renal cortex that synthesize endogenous mouse erythropoietin. These studies demonstrate that multiple erythropoietin gene regulatory elements control cell-type-specific expression and inducibility by a fundamental physiologic stimulus, hypoxia.
![Research paper thumbnail of Localization of cells producing erythropoietin in murine liver by in situ hybridization [see comments]](https://attachments.academia-assets.com/5546328/thumbnails/1.jpg)
](…, Jan 1, 1991
In situ hybridization using antisense RNA probes was used t o localize cells that produce erythro... more In situ hybridization using antisense RNA probes was used t o localize cells that produce erythropoietin (EPO) in the livers of anemic transgenic mice expressing the human EPO gene and in livers of anemic nontransgenic mice. In transgenic mice bled from a hematocrit of 55% to one of 10%. hepatocytes surrounding central veins synthesized large amounts of human EPO mRNA. EPO-producing cells were very rare in the area of portal triads. In transgenic mice bled t o a hematocrit of 20%. a similar number and distribution of cells contained human EPO mRNA as was found with a 10% hematocrit, but the cells were less heavily labeled, indicating increased EPO production per cell at 10% hematocrit as compared with 20% hematocrit. No human EPO mRNA was detected in the kidneys of anemic transgenic mice, although endogenous murine EPO mRNA was strongly expressed in cortical intersti-IDNEY AND LIVER are the only organs known to K produce erythropoietin (EPO) in adult mammals.'-5
British Journal of Haematology, 1992
Experimental hematology, 1988
Kidney …, Jan 1, 1993
Distribution of erythropoietin producing cells in rat kidneys during hypoxic hypoxia. We have use... more Distribution of erythropoietin producing cells in rat kidneys during hypoxic hypoxia. We have used in situ hybridization to determine the localization and distribution of cells expressing the erythropoietin (EPO) gene in kidneys of rats exposed to reduced oxygen tensions to characterize the control of renal EPO formation during hypoxic hypoxia. Animals were subjected to severe hypoxia (7.5% 02) for4, 8 and
The Journal of cell biology, Jan 1, 1989
We have used murine splenic erythrolasts 1. Abbreviatio~ used in this paper: EP, erythropoietin; ... more We have used murine splenic erythrolasts 1. Abbreviatio~ used in this paper: EP, erythropoietin; F-actin, filamentous actin; FVA cells, murine splenic erythroblasts infected with the anemiainducing strain of Friend virus; MEL, murine erythroleukemia; PHEM buffer, 60 mM 1,4 Pipes, 24 mM Hepes, 10 mM EGTA, 2 mM MgCI2, 0.5 mM PMSF (pH 6.9).
British Journal of Haematology, 1996
Contributions to nephrology
Seminars in nephrology, 1993
Contributions to nephrology, 1989
Seminars in hematology, 1991
Klinische Wochenschrift, 1991
The kidney is the main site of erythropoietin (EPO) formation. Oxygen sensing in the kidney itsel... more The kidney is the main site of erythropoietin (EPO) formation. Oxygen sensing in the kidney itself plays a major role in the control of EPO synthesis. By in situ hybridization it has been established that the EPO-producing cells are situated in the interstitium of the cortical labyrinth, but they have not been precisely identified. Morphological findings provide new insights into the location and mechanism of oxygen sensing in the kidney. In addition to causing an increase in the number of cells containing EPO messenger RNA, anemia provokes structural changes exclusively in the cortical labyrinth. Specifically, the fibroblasts become enlarged and show increased activity of 5'-nucleotidase, and the $1 segment of the proximal tubule shows similar alterations as in various models of hypoxia. Thus, structures that are situated in the close vicinity of the EPO-producing cells appear to be sensitive to decreased oxygen delivery.
Future Oncology, 2014
Breast cancer is one of the most prevalent cancers in women worldwide [1,2] and is the most commo... more Breast cancer is one of the most prevalent cancers in women worldwide [1,2] and is the most common cause of cancer deaths in women [2]. The deaths related to breast cancer are not due to the primary tumors, but are due to the metastases to the lungs, brain and bone [3,4]. Therefore, treatment and management to block metastases is an important target for therapy that would improve survival in breast cancer patients.
Applied Radiation and Isotopes, 2008
Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antige... more Clinical immunolocalization has been attempted by others with an anti-Thomsen-Friedenreich antigen (TF-Ag) mAb that bound both alpha-and beta-linked TF-Ag. In this report, 124 I-labeled mAb JAA-F11 specific for alpha-linked TF-Ag showed higher tumor specificity in in vivo micro-positron emission tomography (micro-PET) of the mouse mammary adenocarcinoma line, 4T1, showing no preferential uptake by the kidney. Labeled product remained localized in the tumor for at least 20 days. Glycan array analysis showed structural specificity of the antibody. r
Breast cancer is one of the most prevalent cancers in women worldwide [1, and is the most common ... more Breast cancer is one of the most prevalent cancers in women worldwide [1, and is the most common cause of cancer deaths in women . The deaths related to breast cancer are not due to the primary tumors, but are due to the metastases to the lungs, brain and bone . Therefore, treatment and management to block metastases is an important target for therapy that would improve survival in breast cancer patients.
Cell and tissue research, 1987
The Journal of cell biology, Jan 1, 1988
Spectrin-like proteins are found in a wide 9 The Rockefeller University Press,
Proceedings of the …, Jan 1, 1991
Synthesis of erythropoietin, the primary humoral regulator of erythropoiesis, in liver and kidney... more Synthesis of erythropoietin, the primary humoral regulator of erythropoiesis, in liver and kidney is inducible by anemia or hypoxia. Analysis of human erythropoietin gene expression in transgenic mice revealed that sequences located 6-14 kilobases 5' to the gene direct expression to the kidney, whereas sequences within the immediate 3'flanking region control hepatocyte-specific expression. Human erythropoietin transcription initiation sites were differentially utilized in liver and kidney. Inducible transgene expression was precisely targeted to peritubular interstitial cells in the renal cortex that synthesize endogenous mouse erythropoietin. These studies demonstrate that multiple erythropoietin gene regulatory elements control cell-type-specific expression and inducibility by a fundamental physiologic stimulus, hypoxia.
…, Jan 1, 1991
In situ hybridization using antisense RNA probes was used t o localize cells that produce erythro... more In situ hybridization using antisense RNA probes was used t o localize cells that produce erythropoietin (EPO) in the livers of anemic transgenic mice expressing the human EPO gene and in livers of anemic nontransgenic mice. In transgenic mice bled from a hematocrit of 55% to one of 10%. hepatocytes surrounding central veins synthesized large amounts of human EPO mRNA. EPO-producing cells were very rare in the area of portal triads. In transgenic mice bled t o a hematocrit of 20%. a similar number and distribution of cells contained human EPO mRNA as was found with a 10% hematocrit, but the cells were less heavily labeled, indicating increased EPO production per cell at 10% hematocrit as compared with 20% hematocrit. No human EPO mRNA was detected in the kidneys of anemic transgenic mice, although endogenous murine EPO mRNA was strongly expressed in cortical intersti-IDNEY AND LIVER are the only organs known to K produce erythropoietin (EPO) in adult mammals.'-5
British Journal of Haematology, 1992
Experimental hematology, 1988
Kidney …, Jan 1, 1993
Distribution of erythropoietin producing cells in rat kidneys during hypoxic hypoxia. We have use... more Distribution of erythropoietin producing cells in rat kidneys during hypoxic hypoxia. We have used in situ hybridization to determine the localization and distribution of cells expressing the erythropoietin (EPO) gene in kidneys of rats exposed to reduced oxygen tensions to characterize the control of renal EPO formation during hypoxic hypoxia. Animals were subjected to severe hypoxia (7.5% 02) for4, 8 and
The Journal of cell biology, Jan 1, 1989
We have used murine splenic erythrolasts 1. Abbreviatio~ used in this paper: EP, erythropoietin; ... more We have used murine splenic erythrolasts 1. Abbreviatio~ used in this paper: EP, erythropoietin; F-actin, filamentous actin; FVA cells, murine splenic erythroblasts infected with the anemiainducing strain of Friend virus; MEL, murine erythroleukemia; PHEM buffer, 60 mM 1,4 Pipes, 24 mM Hepes, 10 mM EGTA, 2 mM MgCI2, 0.5 mM PMSF (pH 6.9).