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This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa ... more This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18.The antimicrobial susceptibility test shows, streptomycin, ciprofloxacin, pefloxicin and tarivid to be effective in the treatment of urinary tract infection against Pseudomonas aeruginosa and Proteus mirabilis but were resistant to augmentin. Proper awareness on personal hygiene, routine sample culture and antibiotic sensitivity profiling would play a significant role in reducing the incidence of urinary tract infection.

COMPARATIVE BACTERIOLOGICAL ANALYSIS OF FROZENAND FRESH CHICKEN MEATS SOLD, 2021

Foodborne pathogens are serious public health food production and healthcare while poultry are of... more Foodborne pathogens are serious public health food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken me old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 10 CFU/g and 3.12 x 10 6 CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 a slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus a samples while freshly slaughtered chicken meat samples revealed Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

Habibu Usman Abdu and Aisha Mustapha Abubakar Department of Microbiology, Bayero University Kano ... more Habibu Usman Abdu and Aisha Mustapha Abubakar Department of Microbiology, Bayero University Kano *Corresponding author: ABSTRACT Foodborne pathogens are serious public health food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken me old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 10 CFU/g and 3.12 x 10 CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 a slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylob...

International Journal of Scientific & Technology Research, 2017

this study was carried out to assess the level of susceptibility or resistance of sibling species... more this study was carried out to assess the level of susceptibility or resistance of sibling species of Anopheles gambiae complex from Auyo in Jigawa state between 2013 and 2014 to three classes of insecticides approved by World Health Organization (WHO) for vector control. A. gambiae larvae collected from ecologically contrasting breeding sites were reared to adults in the laboratory. Adults from the F1 progeny were assayed for resistance using the WHO adult insecticide susceptibility bioassay protocol. The mosquitoes were exposed to DDT, Permethrin and Bendiocarb belonging to 3 classes of insecticides approved by WHOPES for malaria vector control. The individual sibling species were identified molecularly and the molecular resistance mechanisms characterized by SINE and kdr PCR methods respectively. The An. gambiae populations tested were highly resistant to DDT and permethrin insecticides but less resistant to bendiocarb. Analysis of the genetic composition of the vector population ...

Glutathione (GSH) is widely distributed among all living organisms and associated with diverse fu... more Glutathione (GSH) is widely distributed among all living organisms and associated with diverse functions that include maintaining redox homeostasis and detoxification of xenobiotic compounds. The level of GSH within the cell varies with stress often involving changes in GSH content, which is consumed in reactions that protect the cell leading to formation of oxidized GSH (GSSG). Mosquitoes breeding in habitats located at agricultural and residential ecosystems may encounter different kinds of pesticides. Enzyme recycling assay method was used to determine glutathione levels in adults Anopheles gambiae (An. gambiae) reared from larvae collected from agricultural (zone A) and residential (zone B) sites. More than 70% of the GSH occur in the reduced form across the sampling sites. Significantly, higher levels of the oxidized form of GSH were recorded in mosquitoes from agricultural compared to residential sites. This may possibly be an adaptive response to strong selection pressure due...

This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa ... more This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18.The antimicrobial susceptibility test shows, streptomycin, ciprofloxacin, pefloxicin and tarivid to be effective in the treatment of urinary tract infection against Pseudomonas aeruginosa and Proteus mirabilis but were resistant to augmentin. Proper awareness on personal hygiene, routine sample culture and antibiotic sensitivity profiling would play a significant role in reducing the incidence of urinary tract infection.

ABSTRACT This study was carried out to determine the distribution of uro-pathogens Pseudomonas ae... more ABSTRACT This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18....

ABSTRACT: The study was conducted to determine various bacterial species responsible for the cont... more ABSTRACT: The study was conducted to determine various bacterial species responsible for the contamination of leafy vegetables in Kano metropolis. Three leafy vegetable samples namely; Brassica olerencea (cabbage), Lactuca sativa (Lettuce) and Spinacia olerenceae (Spinach) were obtained from different markets. Bacterial species isolated were Escherichia coli, Pseudomonas aeruginosa, Staphylococcus and Streptococcus spps. The results have shown that out of the samples examined Staphylococcus sp accounts for a high percentage of occurrences with 42.5% followed by Escherichia coli having 27.9%, then Pseudomonas aeruginosa with 22% and 7.5% for Streptococcus which has been the least. Therefore, consumption of these types of vegetables unhygienically paves way for ingestion of considerable numbers of human pathogenic bacteria. This ultimately results in establishment and manifestation of diseases in the final host. Key word: Bacterial contamination, Lactuca sativa, Spinacea olerencea, Br...

Journal of Biotechnology, 2015

ABSTRACT Abstract Anopheles gambiae (An. gambiae) is the principal malaria vector in Africa, wher... more ABSTRACT Abstract Anopheles gambiae (An. gambiae) is the principal malaria vector in Africa, where vector control measures involve the use of insecticides. The development of insecticides resistance mitigates these approaches. Glutathione (GSH) is widely distributed among all living organisms, and is associated with detoxification pathways, especially the Glutathione S- transferases (GSTs). Its direct involvement and relevance in insecticide resistance in An. gambiae has not been determined. Thus, this work examines the contribution of GSH, its biosynthetic genes (GCLM, GCLC) and their possible transcriptional regulator (Nrf2) in insecticide resistance in An. gambiae. We use bioinformatics, bioassay and molecular techniques to investigate. An. gambiae s.l. studied here were highly resistant to DDT and permethrin but less resistant to bendiocarb. The levels of total, reduced and oxidized GSH were higher in mosquitoes from agricultural sites than those from residential sites. Increased oxidized GSH levels relate with higher insecticides resistance. The expression of GCLM, GCLC and Nrf2 were up-regulated in adults An. gambiae raised from agricultural field-caught larvae when challenged with insecticide. The increased expression levels of these genes and also GSH levels in this population suggest their roles in the response and adaptation of An. gambiae to insecticide challenges. There exists the feasibility of using GSH status in An. gambiae to monitor adaptation and resistance to insecticides. Keywords: Anopheles gambiae, Bioinformatics, Glutathione, Genes, Insecticides.

Journal of Biotechnology, 2014

ABSTRACT Investigating the role of glutathione and glutathione biosynthetic enzymes in the adapta... more ABSTRACT Investigating the role of glutathione and glutathione biosynthetic enzymes in the adaptation of Anopheles gambiae to insecticides Habibu Usman Abdu, , Andrew Spiers, Yusuf Deeni Show more DOI: 10.1016/j.jbiotec.2014.07.310 Get rights and content Glutathione (GSH) plays a central role in the detoxification of xenobiotics. The GSH biosynthetic gene γ-glutamylcyteine ligase modifier subunit (GCLM) is regulated by nuclear factor-like 2 (Nrf2) that protects cell from oxidative stress. To examine the molecular mechanism and the role of GSH in the adaptation of Anopheles gambiae mosquito to insecticides, bioinformatics analyses of GCLM promoter region was performed. Also bioassay carried out on mosquito larvae collected from north western Nigeria where insecticides are used for agriculture or against mosquitoes. Two to five day old adult mosquitoes were tested for susceptibility to insecticides using standard WHO procedures. GSH levels were determined. Insilico analyses of GCLM promoter region revealed several putative transcription factor binding sites, including Nrf2 binding sites, which may be involved in its transcriptional regulation. Results indicate 6–54% (DDT and pyrethroids), 74–95% (Bendiocarb) mortality rates among the mosquitoes tested. Reduced GSH levels were found to be lower in mosquitoes from agricultural sites than those from residential sites. The decreased reduced GSH in mosquitoes from agricultural sites indicates an interaction of insecticides with GSH. Extensive agricultural practice is suspected as a source for selection pressure for the adaptation to insecticides in Anopheles gambiae.

Abstract: This Experiment was carried out in the screen house of the International Institute of T... more Abstract: This Experiment was carried out in the screen house of the International Institute of Tropical Agriculture (IITA) Kano station to study the leaf relative water content and yield of nine cowpea (Vigna unguiculata (L) Walp.) varieties to water stress. Data were collected on the following criteria, number of leaves, leaf area (LA), leaf relative water content (LRWC), and agronomic traits. The experimental materials were arranged in randomize complete block design with three treatments which include unstressed, vegetative stage and flowering stage of water stress. The results indicate that Leaf Relative Water Content (LRWC) of the water stressed genotypes were lower than the unstressed genotypes. At vegetative stage of water stress only 22.22% had an increase in their leaf water content and 77.78% recorded reduction in their LRWC. The highest reduction was exhibited by IT97K-568-19 with 9.5% and lowest reduction was recorded 0.1% by TVU-7778. 55.55% of the genotypes at floweri...

This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa ... more This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18.The antimicrobial susceptibility test shows, streptomycin, ciprofloxacin, pefloxicin and tarivid to be effective in the treatment of urinary tract infection against Pseudomonas aeruginosa and Proteus mirabilis but were resistant to augmentin. Proper awareness on personal hygiene, routine sample culture and antibiotic sensitivity profiling would play a significant role in reducing the incidence of urinary tract infection.

COMPARATIVE BACTERIOLOGICAL ANALYSIS OF FROZENAND FRESH CHICKEN MEATS SOLD, 2021

Foodborne pathogens are serious public health food production and healthcare while poultry are of... more Foodborne pathogens are serious public health food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken me old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 10 CFU/g and 3.12 x 10 6 CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 a slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus a samples while freshly slaughtered chicken meat samples revealed Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

Habibu Usman Abdu and Aisha Mustapha Abubakar Department of Microbiology, Bayero University Kano ... more Habibu Usman Abdu and Aisha Mustapha Abubakar Department of Microbiology, Bayero University Kano *Corresponding author: ABSTRACT Foodborne pathogens are serious public health food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken me old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 10 CFU/g and 3.12 x 10 CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 a slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylob...

International Journal of Scientific & Technology Research, 2017

this study was carried out to assess the level of susceptibility or resistance of sibling species... more this study was carried out to assess the level of susceptibility or resistance of sibling species of Anopheles gambiae complex from Auyo in Jigawa state between 2013 and 2014 to three classes of insecticides approved by World Health Organization (WHO) for vector control. A. gambiae larvae collected from ecologically contrasting breeding sites were reared to adults in the laboratory. Adults from the F1 progeny were assayed for resistance using the WHO adult insecticide susceptibility bioassay protocol. The mosquitoes were exposed to DDT, Permethrin and Bendiocarb belonging to 3 classes of insecticides approved by WHOPES for malaria vector control. The individual sibling species were identified molecularly and the molecular resistance mechanisms characterized by SINE and kdr PCR methods respectively. The An. gambiae populations tested were highly resistant to DDT and permethrin insecticides but less resistant to bendiocarb. Analysis of the genetic composition of the vector population ...

Glutathione (GSH) is widely distributed among all living organisms and associated with diverse fu... more Glutathione (GSH) is widely distributed among all living organisms and associated with diverse functions that include maintaining redox homeostasis and detoxification of xenobiotic compounds. The level of GSH within the cell varies with stress often involving changes in GSH content, which is consumed in reactions that protect the cell leading to formation of oxidized GSH (GSSG). Mosquitoes breeding in habitats located at agricultural and residential ecosystems may encounter different kinds of pesticides. Enzyme recycling assay method was used to determine glutathione levels in adults Anopheles gambiae (An. gambiae) reared from larvae collected from agricultural (zone A) and residential (zone B) sites. More than 70% of the GSH occur in the reduced form across the sampling sites. Significantly, higher levels of the oxidized form of GSH were recorded in mosquitoes from agricultural compared to residential sites. This may possibly be an adaptive response to strong selection pressure due...

This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa ... more This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18.The antimicrobial susceptibility test shows, streptomycin, ciprofloxacin, pefloxicin and tarivid to be effective in the treatment of urinary tract infection against Pseudomonas aeruginosa and Proteus mirabilis but were resistant to augmentin. Proper awareness on personal hygiene, routine sample culture and antibiotic sensitivity profiling would play a significant role in reducing the incidence of urinary tract infection.

ABSTRACT This study was carried out to determine the distribution of uro-pathogens Pseudomonas ae... more ABSTRACT This study was carried out to determine the distribution of uro-pathogens Pseudomonas aeruginosa and Proteus mirabilis and their antimicrobial susceptibility pattern from female patients attending Muhammad Abdullahi Wase Specialist Hospital, Kano, Nigeria, with suspected urinary tract infection. Between July to October, 2012 mid-stream urine samples from 200 patients suspected of urinary tract infection were collected for routine culture and antibiotic susceptibility testing and were processed using standard laboratory methods. Out of the 200 samples, 39% (78) were positives while 61% (122) were negatives. Gram negative bacteria were the predominant isolates. Among them Escherichia coli was the most frequent isolate (33.3%), then Pseudomonas aeruginosa (26.9%), Proteus mirabilis(21.7%), Klebsiella species (8.97%), and Proteus vulgaris (8.97%).The infections caused by Proteus mirabilis and Pseudomonas aeruginosa were more prevalent in adult females especially at the age ≥18....

ABSTRACT: The study was conducted to determine various bacterial species responsible for the cont... more ABSTRACT: The study was conducted to determine various bacterial species responsible for the contamination of leafy vegetables in Kano metropolis. Three leafy vegetable samples namely; Brassica olerencea (cabbage), Lactuca sativa (Lettuce) and Spinacia olerenceae (Spinach) were obtained from different markets. Bacterial species isolated were Escherichia coli, Pseudomonas aeruginosa, Staphylococcus and Streptococcus spps. The results have shown that out of the samples examined Staphylococcus sp accounts for a high percentage of occurrences with 42.5% followed by Escherichia coli having 27.9%, then Pseudomonas aeruginosa with 22% and 7.5% for Streptococcus which has been the least. Therefore, consumption of these types of vegetables unhygienically paves way for ingestion of considerable numbers of human pathogenic bacteria. This ultimately results in establishment and manifestation of diseases in the final host. Key word: Bacterial contamination, Lactuca sativa, Spinacea olerencea, Br...

Journal of Biotechnology, 2015

ABSTRACT Abstract Anopheles gambiae (An. gambiae) is the principal malaria vector in Africa, wher... more ABSTRACT Abstract Anopheles gambiae (An. gambiae) is the principal malaria vector in Africa, where vector control measures involve the use of insecticides. The development of insecticides resistance mitigates these approaches. Glutathione (GSH) is widely distributed among all living organisms, and is associated with detoxification pathways, especially the Glutathione S- transferases (GSTs). Its direct involvement and relevance in insecticide resistance in An. gambiae has not been determined. Thus, this work examines the contribution of GSH, its biosynthetic genes (GCLM, GCLC) and their possible transcriptional regulator (Nrf2) in insecticide resistance in An. gambiae. We use bioinformatics, bioassay and molecular techniques to investigate. An. gambiae s.l. studied here were highly resistant to DDT and permethrin but less resistant to bendiocarb. The levels of total, reduced and oxidized GSH were higher in mosquitoes from agricultural sites than those from residential sites. Increased oxidized GSH levels relate with higher insecticides resistance. The expression of GCLM, GCLC and Nrf2 were up-regulated in adults An. gambiae raised from agricultural field-caught larvae when challenged with insecticide. The increased expression levels of these genes and also GSH levels in this population suggest their roles in the response and adaptation of An. gambiae to insecticide challenges. There exists the feasibility of using GSH status in An. gambiae to monitor adaptation and resistance to insecticides. Keywords: Anopheles gambiae, Bioinformatics, Glutathione, Genes, Insecticides.

Journal of Biotechnology, 2014

ABSTRACT Investigating the role of glutathione and glutathione biosynthetic enzymes in the adapta... more ABSTRACT Investigating the role of glutathione and glutathione biosynthetic enzymes in the adaptation of Anopheles gambiae to insecticides Habibu Usman Abdu, , Andrew Spiers, Yusuf Deeni Show more DOI: 10.1016/j.jbiotec.2014.07.310 Get rights and content Glutathione (GSH) plays a central role in the detoxification of xenobiotics. The GSH biosynthetic gene γ-glutamylcyteine ligase modifier subunit (GCLM) is regulated by nuclear factor-like 2 (Nrf2) that protects cell from oxidative stress. To examine the molecular mechanism and the role of GSH in the adaptation of Anopheles gambiae mosquito to insecticides, bioinformatics analyses of GCLM promoter region was performed. Also bioassay carried out on mosquito larvae collected from north western Nigeria where insecticides are used for agriculture or against mosquitoes. Two to five day old adult mosquitoes were tested for susceptibility to insecticides using standard WHO procedures. GSH levels were determined. Insilico analyses of GCLM promoter region revealed several putative transcription factor binding sites, including Nrf2 binding sites, which may be involved in its transcriptional regulation. Results indicate 6–54% (DDT and pyrethroids), 74–95% (Bendiocarb) mortality rates among the mosquitoes tested. Reduced GSH levels were found to be lower in mosquitoes from agricultural sites than those from residential sites. The decreased reduced GSH in mosquitoes from agricultural sites indicates an interaction of insecticides with GSH. Extensive agricultural practice is suspected as a source for selection pressure for the adaptation to insecticides in Anopheles gambiae.

Abstract: This Experiment was carried out in the screen house of the International Institute of T... more Abstract: This Experiment was carried out in the screen house of the International Institute of Tropical Agriculture (IITA) Kano station to study the leaf relative water content and yield of nine cowpea (Vigna unguiculata (L) Walp.) varieties to water stress. Data were collected on the following criteria, number of leaves, leaf area (LA), leaf relative water content (LRWC), and agronomic traits. The experimental materials were arranged in randomize complete block design with three treatments which include unstressed, vegetative stage and flowering stage of water stress. The results indicate that Leaf Relative Water Content (LRWC) of the water stressed genotypes were lower than the unstressed genotypes. At vegetative stage of water stress only 22.22% had an increase in their leaf water content and 77.78% recorded reduction in their LRWC. The highest reduction was exhibited by IT97K-568-19 with 9.5% and lowest reduction was recorded 0.1% by TVU-7778. 55.55% of the genotypes at floweri...