Afaf Amin | Cairo University (original) (raw)

Papers by Afaf Amin

Planta Medica, 2014

ABSTRACT Nymphoides indica (L.) Kuntze (Menyanthaceae) is traditionally used in many ethnomedicin... more ABSTRACT Nymphoides indica (L.) Kuntze (Menyanthaceae) is traditionally used in many ethnomedicinal formulations in Asia [1]; however, scientific data reporting its constituents are poor. N. indica leaves were macerated with methanol 90% and subsequently extracted using various solvents. After successful extraction, each fraction was evaluated for various claimed bioactivities such as antibacterial, antifungal, cytotoxic, antioxidant and antiglycation activity. The microorganism used included S. aureus, E. coli, C. albicans, M. canis and MRC-5 cells. The methanol 90% fraction showed notable cytotoxic properties (IC50 38.9 µg/mL). Antifungal and antibacterial activities were observed for the methanol 90% (IC50 32 µg/mL) and n-hexane (IC50 19.15 µg/mL) fractions respectively. The ethyl acetate fraction was found to present the highest antioxidant activity (IC50 147 µg/mL) followed by methanol 90% (IC50 211 µg/mL) and chloroform (IC50 380 µg/mL) fractions. The n-butanol fraction had the highest (IC50 32 µg/mL) anti-glycation (Advanced Glycation Endproducts, AGEs) activity, followed by the chloroform (IC50 64 µg/mL), ethyl acetate (IC50 69 µg/mL) and methanol 90% (IC50 86 µg/mL) fractions. All extracts were further fractionated using repeated flash chromatography and analysed using TLC and HPLC-DAD. For the isolation of major compounds, a semi preparative HPLC(RP)-DAD-MS system was used. Subsequently NMR and mass spectra were recorded to elucidate the structure of the isolated compounds. Isolated compounds included flavonoids (1 – 4), seco-cyclopentane glucosides (5 – 7), caumerine (8), phenolic acids (9) and monoterpenes (10 – 11).

Journal of Scientific Research in Science

Pot experiment was carried out during May 2019 at private farm in el Manyial, Cairo, Egypt. The i... more Pot experiment was carried out during May 2019 at private farm in el Manyial, Cairo, Egypt. The investigation target was to find out the difference between using plant growth promoting rhizobacteria (B. cereus, Streptomyces chibaensis, B. megaterium, B. polymyxa) either individual or in combination with each other and mineral fertilization on antimicrobial activity and some phytochemical constitutes of Moringa olifera leaves. The highest values of antimicrobial activity recorded for mineral fertilization on treatment followed by mixed biofertilization treatment (GP4) (B. megaterium, B. polymyxa) against seven foodborne pathogens Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus stolonifer and Botrytis cinera, as compared with the control treatment. Biofertilization treatment (B. megaterium, B. polymyxa) recorded increasing over control in (GP10) by 160.8% and decreasing of chemical fertilization by 5.41% for polyphenol content, also, the antioxidant content shown increasing over the control (GP10) by 81.1% and decreasing of chemical fertilization (GP9) by 5.6% as well as vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 55 days. Biofertilization in (GP4) recorded increasing over control group (GP10) by 48.89%, 27.08% and 58% for vitamin A, C and chlorophyll respectively. Ethyl acetate extract was found to be most potent extract than ethanol and water against all tested microorganism.

Detection of 793/B serotype of infection bronchitis virus from broiler flocks with respiratory si... more Detection of 793/B serotype of infection bronchitis virus from broiler flocks with respiratory signs in west of Mazandran province. Hosseini Aliabad, S.A1 *., Momayez, R2., Mahmodzadeh, M2., Yosefi Amin, A2. 1- Veterinary Department, Faculty of agriculture, Islamic Azad University, Chalous,Iran. 2- Razi Vaccine and Serum Research Institute, Karaj, Iran. *Corresponding Author: erfsat@yahoo.com Abstract For detection of the 793/B serotype of infection bronchitis virus in respiratory infections, 11 samples were taken from broiler flocks with respiratory signs in west of Mazandran province in autumn and winter of 2010. After inoculation of samples into the embryonated SPF chicken eggs, samples were checked for heamagglutination (HA) characteristic by HA test for Newcastle disease (ND) and Avian Influenza (AI) viruses, and then the cases having HA property were tested by ND and AI specific antiserum and evaluated for ND and AI virus by heamagglutination inhibition (HI) test. For identifi...

Zagazig Journal of Pharmaceutical Sciences, Jun 1, 1998

3 Abstract: Newcastle disease virus (NDV) clone 30 was propagated on Vero cells. Plaque forming t... more 3 Abstract: Newcastle disease virus (NDV) clone 30 was propagated on Vero cells. Plaque forming test and minimal cytotoxic concentration at multiplicities of infection (MOI)of 0.01, 0.1 and 1were performed to use 50 minimal concentration of the virus. HeLa cells were infected with an MOI of 1.0. At 1, 3, 5 and 7 days post infection. On the other hand the oncolytic activity of NDV was tested on induced tumor in mice. The minimal lethal concentration was firstly determined to use minimal safe concentration of the virus. Then 40 mice were 50

Journal of Scientific Research in Science

Hydrocolloid gums are extensively used in food industry. Recently hydrocolloid gums especially Gu... more Hydrocolloid gums are extensively used in food industry. Recently hydrocolloid gums especially Gum Arabic (GA) has been widely used as edible coatings to extent shelf life of postharvest fruits and vegetables. The objective of this study was to evaluate the effect of GA edible film application mixed with glycerol and CaCl2as base matrix with all GA films i.e. GA/EDTA,GA/L. paracasei supernatant and GA/EDTA /L. paracasei supernatant in preservation of potato tubers stored at 8°C and 30±5°C for 35 days. Physicochemical analyses including pH, weight loss percentage and total soluble solids percentage (TSS), as well as microbial analysis (total counts of bacteria, mold and yeast and Enterobacteriaceae).The obtained result revealed that the total microbial count was found that GA/EDTA/ L. paracasei supernatant gave a very close results to petroleum coating (wax) and the best results compared withnoncoated (control) tubers, this was evident through the lower microbial load, better results than other treatments and noncoated tubers such as reducing weight loss, total soluble solids, better control on pH and expansion of the shelf life.

Zagazig Journal of Pharmaceutical Sciences

A significant portion of the agricultural produce in the countries and the world over become unfi... more A significant portion of the agricultural produce in the countries and the world over become unfit for human consumption due to mycotoxins contamination of grains and cereals. The main toxic effects are carcinogenicity, genotoxicity, teratogenicity, nephrotoxicity, hepatotoxicity, reproductive disorders and imunosuppression. This study was done to identify the molds and aflatoxins that contaminate cereal–based baby foods and corn–based snacks products. The most frequent fungal genera found in the samples were Aspergillus, Penicillium, Alternaria, Fusariumand Cladosporium with frequencies of 41, 16, 10, 8 and 3%, respectively. Additionally, the numbers of contaminated cereal–based baby foods samples with AFB1, B2, G1 and G2 were 14, 2, 6 and 4%. Also, 34, 14, 18 and 8% of corn-based snack samples respectively. Ten essentials oils of (cinnamon, cumin, clove, fennel, garlic, lemon grass, marjoram, peppermint, rosemary and thyme) plants using in combating aflatoxigenic mold A. flavus gr...

The purpose of this study was to estimate the proleolytic activity of subgingival nucroflora of d... more The purpose of this study was to estimate the proleolytic activity of subgingival nucroflora of diabetic and non-diabetic individividuals as an attempt to clarify the role of these enzymes which are produced by some Gram + ve and Cram - ve bacteria in the pathogenesis of periodontal tissue destruction in patients suffering from. diabetes mellitus. Clinical indices and microbiological parameters were determined of39 adult patients , their age ranged from 35-65, 18 diabetic (non-insuline dependent diabetes mellitus) and 21 non-diabetics. The clinical results demonstrated the significant increase in all gineival clinical parameters in diabetics comparing with the non-diabetics, Microblologically, staphy - lococcus aureus, micrococcus sp.. Gram - ve bacilli and yeasts showed higher percentage of isolates in diabetic patients than in non-diabetic patients. Thus diabetic status may, not only affect the clinical aspect of the gingival tissue of the diabetic patients, but may also modify mi...

International Journal of Advanced Research

COVID-19 is a new strain of coronavirus to which humans have not got immunity. It originated in C... more COVID-19 is a new strain of coronavirus to which humans have not got immunity. It originated in China and has quickly spread around the world.It is a disease caused by an infection by SARS-CoV-2 virus, first identified in the city of Wuhan, in Chinas Hubei province in December 2019. COVID-19 was previously known as 2019 Novel Coronavirus (2019-nCoV) respiratory disease before the World Health Organization (WHO) declared the official name as COVID-19 in February 2020. Like the other coronaviruses, the SARS-CoV-2 virus primarily causes respiratory tract infections, and the severity of the COVID-19 disease can range from mild to fatal.

Egyptian Journal of Nutrition and Health, 2006

Twenty eight crude water extracts of four Egyptian plants: Hibiscus sabdariffa (Karkade) , Tamari... more Twenty eight crude water extracts of four Egyptian plants: Hibiscus sabdariffa (Karkade) , Tamarindus indica (Tamarind) t Glycyrrhiza glabra (Liquorice) and Ceratonia siliqua (Carob) , were tested for their antibacterial activities against 10 common pathogenic bacteria,Bacillus cereus, Shigella flexenary and Escherichia coli showed sensitivity to the 28 extracts. Shigella sp. showed sensitivity to 24 extracts while Pseudomonas sp. and Salmonella typhimurium were sensitive to 23 extracts. Salmonella paratyphi and Aerobacter aerogenes showed sensitivity to 22 extracts, Serratia marcesence and Aeromonas hydrophila showed sensitivity to 20 extracts. Karkade exhibited marked antibacterial activity against all the 10 organisms. E. coli and Shigella flexenary had no observed sensitivity to Liquorice extracts. Aerobacter aerogenes was the only one which had no observed sensitivity to Tamarind extracts. All the extracts of Carob were effective against only two pathogens:Bacillus cereus and Shigella flexenary. The results support the traditional uses of extracts of these plants for the management of bacterial infections and for the development of antibacterial agents for the preservation of foods. Introduction: Herbal medicine depends on the action of non-essential nutrients and the phytochemical (Walker, 2006).Liquorice has been used as medicine in China for centuries. It may be taken by mouth to treat stomach conditions such as ulcers and respiratory conditions as bronchitis and it may be anti-infective and anti-inflammatory (Frus-Moller et al., 2002).Two compounds from Liquorice inhibit the growth of Streptococcus mutans , the primary bacteria responsible for causing cavities. In fact, Liquorice roots antimicrobial activity was seen in a number of experiments (Sahelian, R., 2005). Polyphenols in Carob possess antibacterial and anti-inflammatory effects. Substantial in vitro and animal studies support the beneficial effects of polyphenols in many gastrointestinal diseases (

African Journal of Biological Sciences

Mycological survey was carried out to identify the molds and aflatoxins present in cereal -derive... more Mycological survey was carried out to identify the molds and aflatoxins present in cereal -derived products. One hundred samples of cereal -derived products (50 samples of cereal -based baby foods, for infants and young children, and 50 samples of corn -based snacks), purchased from supermarkets and small shops in Cairo and El-Qaliubia Governorates, were used for fungal quantification, isolation, identification and analyzed for the presence of aflatoxins (B 1 , B 2 ) using High Performance Liquid Chromatography (HPLC). Aspergillus (41%), Penicillium (16%), Alternaria (10%), Fusarium (8%) and Cladosporium (3%) were the prevalent genera in the samples. Aflatoxin B 1 (AFB 1 ) could be detected in 14% of the cereal samples purchased from Cairo and in 40 %, 28% corn -based snacks samples purchased from Cairo and El-Qaliubia respectively. Aflatoxin B 2 (AFB 2 ) could be detected in 2% of the cereal samples of Cairo and in 4%, 24% of the corn -based snacks samples of Cairo and El-Qaliubia respectively. The results revealed the level of AFB 1 in 13% of the samples examined was higher than the EU permission limit (2 µg kg -1 ) and the daily intake, for all ages, of the products of corn -based snacks (from 30 to 50 g), contaminated with AFB 1 is ranging from 0.42 to 23.75 ng kg -1 bw per day, it is higher than the PMTDI (Provisional Maximum Tolerable Daily Intake,1.0 ng AFB 1 kg -1 body weight for adult and children without hepatitis B), also there is a significant risk for babies (6, 12 and 24 months), who occasionally consumes a cereal -based food (30 g per day), from the most contaminated sample, the estimated daily intake would be from 6.66 to 11.1 ng k g -1 bw per day and even in consumption of the less contaminated sample, the estimated daily intake is from 1.62 to 2.7 ng k g -1 bw per day. The food-borne mycotoxins with the greatest significance for human health in developing countries is aflatoxins thus, routine measurements of the toxin levels in foods should be carried out to prevent their harmful effects on health.

Planta Medica, 2014

Journal of Scientific Research in Science

Zagazig Journal of Pharmaceutical Sciences, Jun 1, 1998

Journal of Scientific Research in Science

Zagazig Journal of Pharmaceutical Sciences

International Journal of Advanced Research

Egyptian Journal of Nutrition and Health, 2006

African Journal of Biological Sciences