Ashraf M Abu-Seida | Cairo University (original) (raw)

Papers by Ashraf M Abu-Seida

Research paper thumbnail of Collagen membrane and L-PRF with Xenogeneic bone block for vertical ridge augmentation: An Experimental study in a canine model

Ain Shams Dental Journal, Jun 1, 2021

Research paper thumbnail of Comparison of the Sedative, Analgesic, Behavioral, Hematological and Serum Biochemistry Effects of Four Analgesics in Dogs

Advances in Animal and Veterinary Sciences, 2022

Research paper thumbnail of Treatment of a congenital lateral patellar luxation by recession trochleoplasty in a donkey foal

Equine Veterinary Education, Dec 11, 2020

Research paper thumbnail of Treatment challenges in equine fibrosarcomas: Can the harmonic scalpel help in the treatment success?

Equine Veterinary Education, Jun 25, 2023

SummaryFibrosarcoma is relatively uncommon aggressive neoplasm, representing less than 3% of all ... more SummaryFibrosarcoma is relatively uncommon aggressive neoplasm, representing less than 3% of all neoplasms recorded in horses. Successful treatment of fibrosarcoma can be challenging in equine and recurrence is a common late complication. The accompanying paper by Bellitto et al. (2023, Equine Veterinary Education, 10.1111/eve.13808) illustrates the successful surgical treatment of a large fibrosarcoma over the medial and plantar aspect of the left distal limb in an 18‐year‐old Warmblood gelding. The authors report on the success of treatment after surgical excision of the tumour under general anaesthesia using a harmonic scalpel. The gelding recovered well after 1 year of surgery with no recurrence at that time. Further clinical studies are recommended to compare the harmonic scalpel with other modalities like surgical lasers and conventional sharp excision for treatment of fibrosarcoma in horses as well as to understand the potential benefits of using the harmonic scalpel when treating infiltrative tumours such as fibrosarcoma. The publication of this case report in Equine Veterinary Education is of clinical importance to equine practitioners when treating equine infiltrative neoplasms such as fibrosarcomas.

Research paper thumbnail of A novel surgical technique for treatment of cervical vertebral stenotic myelopathy (wobbler syndrome) in a filly

Journal of Equine Veterinary Science, Jul 1, 2023

Research paper thumbnail of Histopathological pulp response of dog's teeth capped with biosealer and biodentine: An in vivo study

Saudi Endodontic Journal, Sep 1, 2020

Introduction: The aim of this study was to evaluate the pulpal response after pulp capping using ... more Introduction: The aim of this study was to evaluate the pulpal response after pulp capping using either biodentine (BD) or tech biosealer capping (TBC) in the dog model. Materials and Methods: Class V cavities were carried out on 45 teeth in three mongrel dogs. The dental pulp was exposed in 30 teeth (2 experimental groups) and left unexposed in 15 teeth (control group). The cavities of the experimental groups were capped with either BD (n = 15 teeth) or TBC (n = 15 teeth). All cavities in the experimental and control groups were restored with resin-modified glass ionomer. Dentin bridge formation, architecture of the odontoblastic layers, and signs of inflammation were assessed after 1, 2, and 3 months using the computer image analyzer. Results: The BD group exhibited a thick newly formed reparative dentin bridge completely closing the exposure site with cell inclusions and mineralization, variable numbers of odontoblast-like cells, preserved pulp tissue, marked numerous collagen fibers, and blood vessels. While the TBC group exhibited an incomplete newly formed reparative dentin bridge with tunnel defect, vacuolated odontoblasts, complete pulp degeneration with multiple edematous spaces, hyperemic blood vessels, extravasated red blood cells, multiple calcified structures scattered just beneath the dentin bridge and through the pulp tissue, and newly ill-defined odontoblasts. Conclusion: For pulp capping, BD has a better dentin bridge formation and pulp preservation than TBC in the dog model.

Research paper thumbnail of Inflammatory response and immunohistochemical characterization of experimental calcium silicate‐based perforation repair material

International Journal of Experimental Pathology, Apr 1, 2022

This study compares the immunohistochemical reaction of a new experimental tricalcium silicate pe... more This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided into three experimental groups (n = 54 teeth each) according to the evaluation period: 1, 2 and 3 months. Each group was further divided into two equal subgroups (n = 27 teeth each) according to the time of repair: immediate repair and delayed repair. Each subgroup was subdivided according to the material used into three experimental subdivisions (n = 8 teeth each): MTA, Biodentine (Septodont) and experimental material, and two control subdivisions: positive control (n = 2 teeth) and negative control (one tooth). Under general anaesthesia, access cavity was done. Cleaning and shaping were performed using ProTaper universal rotary instruments. The canals were obturated using cold lateral compaction technique with Gutta percha and Adseal sealer. Furcation perforations were created then randomly sealed using the three materials either immediately or after one month (delayed repair). Inflammatory cell count and immunohistochemical analysis of osteopontin-positive area fraction were digitally analysed using the ImageJ software. Delayed furcal perforation repair showed significantly higher inflammatory cell count than immediate repair. No significant difference in inflammatory cell count and immunohistochemical analysis was detected between the three tested materials. The immunohistochemical analysis revealed the highest immunopositive area fraction in the 3-month evaluation period. The experimental tricalcium silicate cement performed similarly to Biodentine and MTA regarding the osteopontin expression during perforation repair, suggesting it is a suitable alternative with favourable handling characters.

Research paper thumbnail of Feline Hypertrophic Osteopathy Associated with Congenital Megaesophagus: Two Case Reports and Literature Review

Journal of the Hellenic Veterinary Medical Society, Oct 15, 2020

Research paper thumbnail of Diagnostic and Treatment Challenges for Diaphragmatic Hernia in Equids: A Concise Review of Literature

Journal of Equine Veterinary Science, Nov 1, 2021

Diaphragmatic hernia (DH) represents an acute abdominal crisis, but respiratory symptoms may be o... more Diaphragmatic hernia (DH) represents an acute abdominal crisis, but respiratory symptoms may be occurring. Different types of diaphragmatic hernias (DHs) like typical DH, Morgagni hernia, hiatal hernia and diaphragmatic diverticulum have been recorded in equids. These DHs have various clinical presentations and prognoses. Early diagnosis and prompt surgical repair are highly recommended in order to diminish the ischemic changes to thoracic and abdominal viscera, thus improving the overall prognosis of equids with DH. Over last decade, the prognosis of DH in equids was better than previously believed due to improvements in diagnostic tools, general anesthesia of equids, (particularly those with systemic compromise) and gastrointestinal surgery, development of thoracoscopic techniques and use of staples for surgical repair. Nevertheless, several diagnostic and treatment challenges are still present during management of DH in equids. Diagnostic challenges include inconsistent clinical signs of DH, acute nature of the associated colic and absence of highly qualified diagnostic tools. Increased awareness of the case, good clinical observation and availability of diagnostic tools are recommended to overcome these challenges. Also anesthesia, reduction of large herniated organs and herniorrhaphy are major challenges. Preoperative stabilization, careful monitoring, selection of the most accessible surgical approach and good intraoperative as well as postoperative support are highly recommended to overcome these challenges. Surgical repair, particularly prosthetic herniorrhaphy seems to be effective to close the diaphragmatic defect and prevent recurrences. The present review describes the different types of DHs in equids, their diagnostic and treatment challenges and how to overcome these challenges.

Research paper thumbnail of Regenerative endodontic therapy in mature teeth with necrotic pulp and apical periodontitis using two disinfection protocols

BMC Oral Health, Mar 22, 2023

Research paper thumbnail of First report of nephrocutaneous fistula in a cat

Bulgarian Journal of Veterinary Medicine, 2022

Research paper thumbnail of Luxation of the patella in foals: A treatment challenge

Equine Veterinary Education, Mar 14, 2021

Research paper thumbnail of Biosealer versus Biodentine for direct pulp capping in dog’s teeth: A histopathological evaluation

Research Square (Research Square), Aug 14, 2019

Research paper thumbnail of MRI features of multiple phalangeal disorders in a draft horse – a case report

Veterinarski arhiv

Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness ... more Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness in equines. This case report describes the MRI features of multiple phalangeal disorders in a 9-year-old horse with chronic unilateral forelimb lameness due to severe trauma. A complete case history was taken, and a thorough clinical examination, radiography, ultrasonography and MRI were performed. Radiography and ultrasonography showed limited value. MRI revealed edema of the navicular bursa (NB), distal sesamoidean impar desmitis, DDFT injury at navicular and post-navicular levels, cartilage erosion of the pastern and coffin joints, medial collateral desmitis of the fetlock joint, medial collateral sesamoidean desmitis, and lateral collateral desmitis of the coffin joint. In conclusion, severe trauma may induce multiple phalangeal disorders with severe pain in draft horses, and MRI is superior to other diagnostic imaging modalities, such as radiography and ultrasonography, for diagnosi...

Research paper thumbnail of The impact of membrane perforation and L-PRF for vertical ridge augmentation with a xenogeneic block graft: an experimental study in a canine model

Clinical Oral Investigations

Objectives This study evaluated clinically and histologically the efficacy of modified perforat... more Objectives This study evaluated clinically and histologically the efficacy of modified perforated collagen membrane (PCM) and/or leukocyte- and platelet-rich fibrin (L-PRF) in combination with xenogeneic block bone graft in the vertical alveolar ridge augmentation. Materials and methods Six adult mongrel dogs were enrolled in this randomized blinded study. After defect preparation, xenogeneic screw-fixed block graft was covered by an occlusive collagen membrane in group 1 that represented the control group (Block + CM). In group 2, L-PRF membrane was added first before top coverage by occlusive collagen membrane (Block + L-PRF + CM). Groups 3 (Block + PCM) and 4 (Block + L-PRF + PCM) were identical to the first two groups except that the occlusive collagen membrane was replaced by a perforated one. Following a healing period of 2 months, the dogs were submitted to the surgical reentry phase for clinical and histological evaluation. Results Clinically, no significant differences we...

Research paper thumbnail of The use of Xenogeneic Block Graft and Perforated Collagen Membrane Augmented L-PRF Coverage in Vertical Ridge Augmentation: An Experimental Study in Canine Model

Research Square (Research Square), Jan 19, 2023

Research paper thumbnail of Histopathological and immunohistochemical profiles of pulp tissues in immature dogs’ teeth to two recently introduced pulpotomy materials

Clinical Oral Investigations

Objective The pulpal response to Hoffmann’s Pulpine mineral (PMIN) and Pulpine NE (PNE) was compa... more Objective The pulpal response to Hoffmann’s Pulpine mineral (PMIN) and Pulpine NE (PNE) was compared to mineral trioxide aggregate (MTA) when used as pulpotomy materials in immature permanent teeth in dogs. Materials and methods Immature premolars were randomly divided according to the observation period into three equal groups (n = 24) (10 days, 30 days, and 90 days) then furtherly subdivided into 3 subgroups according to the material used. Histopathological analysis regarding inflammatory cell infiltration and dentin bridge (DB) formation was done. Immunohistochemical analysis was performed using osteopontin marker. Results The results showed that after 90 days, both MTA and PMIN subgroups had 100% complete thick DB without inflammation in 87.5% of the samples, while the PNE subgroup failed to form DB in 37.5% of the samples and 50% of samples showed thin initial DB with heavy inflammation in 62.5% of the samples. There was no significant difference between MTA and PMIN, while the...

Research paper thumbnail of Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)

Research paper thumbnail of Biological Pulp Response of Pulpine, Polyamidoamine Dendrimer and Their Combination in Dogs and their Remineralizing Effect on Carious Affected Human Dentin: A Randomized Clinical Trial

Al-Azhar Dental Journal for Girls, 2021

Purpose: This study was conducted to evaluate the biological pulp response to Pulpine and Polyami... more Purpose: This study was conducted to evaluate the biological pulp response to Pulpine and Polyamidoamine Dendrimer and their combination in dogs and comparing their remineralizing potentials on human caries affected dentin. Materials and Methods: Forty-five teeth in 3 mongrel dogs were randomly divided into 3 groups according to the applied pulp capping material; group A1: Pulpine NE, group A2: PAMAM G3 and group A3: Pulpine and PAMAM. Class V cavities were prepared; the pulps were mechanically exposed and finally restored with resin modified glass ionomer. Histological evaluation was performed after 2 weeks, 1 and 2 months. The in vivo study was designed as a randomized, unicenter, parallel, two arms, double– blind split mouth clinical trial with 1:1 allocation ratio. The subjects who fulfilled the eligibility criteria were asked to participate and signed informed consent. Twenty-eight adhesive occlusal cavities were prepared; infected dentin was removed leaving carious affected de...

Research paper thumbnail of The effect of changing apical foramen diameter on regenerative potential of mature teeth with necrotic pulp and apical periodontitis

Clinical Oral Investigations, 2021

Objective The aim of the present study was to evaluate the regenerative potential of permanent ne... more Objective The aim of the present study was to evaluate the regenerative potential of permanent necrotic mature teeth with different apical foramen diameters in comparison to conventional endodontic treatment. Methods Pulp necrosis and periapical pathosis were induced in premolars (144 roots) in six mongrel dogs. The infected teeth were divided into six groups; conventional root canal treatment (RCT) group prepared up to apical preparation sizes #F3 ProTaper file, regenerative endodontic therapy (RET) groups; groups RET-F3, RET-F4, and RET-F5 where regenerative endodontic procedures were applied after apical preparation up to sizes #F3, F4, and F5 ProTaper file, respectively, control groups; and positive (P) and negative (N) groups included infected and healthy teeth respectively. Each group (12 teeth with 24 roots each) was further subdivided into two subgroups (6 teeth with 12 roots each) according to the evaluation periods 1 and 3 months; each group was equally randomized in the upper and lower jaws during each evaluation period. Results The inflammatory cell counts for the RCT group were statistically significantly higher than that of all RET groups at the first period and only significantly higher than RET-F5 at the second period. The enlargement of the apical foramen to #F5 showed no statistically significant difference with #F4 regarding the inflammatory cell count, tissue in-growth inside the root canal, and percentage of bone resorption. The tissue formed after RET was not true pulp tissue and is considered tissue repair with connective tissue, dilated blood vessels, and cementum-like structures. Conclusion Increasing the apical size diameter was associated with less inflammatory cell count, less percentage of bone resorption, more tissue ingrowth, and more percentage of new hard tissue formation inside root canal. The minimum apical diameter that should be enlarged for RET is 0.4 mm. Clinical relevance RET could be used as an alternative procedure to RCT for the treatment of mature teeth with necrotic pulp and apical periodontitis.

Research paper thumbnail of Collagen membrane and L-PRF with Xenogeneic bone block for vertical ridge augmentation: An Experimental study in a canine model

Ain Shams Dental Journal, Jun 1, 2021

Research paper thumbnail of Comparison of the Sedative, Analgesic, Behavioral, Hematological and Serum Biochemistry Effects of Four Analgesics in Dogs

Advances in Animal and Veterinary Sciences, 2022

Research paper thumbnail of Treatment of a congenital lateral patellar luxation by recession trochleoplasty in a donkey foal

Equine Veterinary Education, Dec 11, 2020

Research paper thumbnail of Treatment challenges in equine fibrosarcomas: Can the harmonic scalpel help in the treatment success?

Equine Veterinary Education, Jun 25, 2023

SummaryFibrosarcoma is relatively uncommon aggressive neoplasm, representing less than 3% of all ... more SummaryFibrosarcoma is relatively uncommon aggressive neoplasm, representing less than 3% of all neoplasms recorded in horses. Successful treatment of fibrosarcoma can be challenging in equine and recurrence is a common late complication. The accompanying paper by Bellitto et al. (2023, Equine Veterinary Education, 10.1111/eve.13808) illustrates the successful surgical treatment of a large fibrosarcoma over the medial and plantar aspect of the left distal limb in an 18‐year‐old Warmblood gelding. The authors report on the success of treatment after surgical excision of the tumour under general anaesthesia using a harmonic scalpel. The gelding recovered well after 1 year of surgery with no recurrence at that time. Further clinical studies are recommended to compare the harmonic scalpel with other modalities like surgical lasers and conventional sharp excision for treatment of fibrosarcoma in horses as well as to understand the potential benefits of using the harmonic scalpel when treating infiltrative tumours such as fibrosarcoma. The publication of this case report in Equine Veterinary Education is of clinical importance to equine practitioners when treating equine infiltrative neoplasms such as fibrosarcomas.

Research paper thumbnail of A novel surgical technique for treatment of cervical vertebral stenotic myelopathy (wobbler syndrome) in a filly

Journal of Equine Veterinary Science, Jul 1, 2023

Research paper thumbnail of Histopathological pulp response of dog's teeth capped with biosealer and biodentine: An in vivo study

Saudi Endodontic Journal, Sep 1, 2020

Introduction: The aim of this study was to evaluate the pulpal response after pulp capping using ... more Introduction: The aim of this study was to evaluate the pulpal response after pulp capping using either biodentine (BD) or tech biosealer capping (TBC) in the dog model. Materials and Methods: Class V cavities were carried out on 45 teeth in three mongrel dogs. The dental pulp was exposed in 30 teeth (2 experimental groups) and left unexposed in 15 teeth (control group). The cavities of the experimental groups were capped with either BD (n = 15 teeth) or TBC (n = 15 teeth). All cavities in the experimental and control groups were restored with resin-modified glass ionomer. Dentin bridge formation, architecture of the odontoblastic layers, and signs of inflammation were assessed after 1, 2, and 3 months using the computer image analyzer. Results: The BD group exhibited a thick newly formed reparative dentin bridge completely closing the exposure site with cell inclusions and mineralization, variable numbers of odontoblast-like cells, preserved pulp tissue, marked numerous collagen fibers, and blood vessels. While the TBC group exhibited an incomplete newly formed reparative dentin bridge with tunnel defect, vacuolated odontoblasts, complete pulp degeneration with multiple edematous spaces, hyperemic blood vessels, extravasated red blood cells, multiple calcified structures scattered just beneath the dentin bridge and through the pulp tissue, and newly ill-defined odontoblasts. Conclusion: For pulp capping, BD has a better dentin bridge formation and pulp preservation than TBC in the dog model.

Research paper thumbnail of Inflammatory response and immunohistochemical characterization of experimental calcium silicate‐based perforation repair material

International Journal of Experimental Pathology, Apr 1, 2022

This study compares the immunohistochemical reaction of a new experimental tricalcium silicate pe... more This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided into three experimental groups (n = 54 teeth each) according to the evaluation period: 1, 2 and 3 months. Each group was further divided into two equal subgroups (n = 27 teeth each) according to the time of repair: immediate repair and delayed repair. Each subgroup was subdivided according to the material used into three experimental subdivisions (n = 8 teeth each): MTA, Biodentine (Septodont) and experimental material, and two control subdivisions: positive control (n = 2 teeth) and negative control (one tooth). Under general anaesthesia, access cavity was done. Cleaning and shaping were performed using ProTaper universal rotary instruments. The canals were obturated using cold lateral compaction technique with Gutta percha and Adseal sealer. Furcation perforations were created then randomly sealed using the three materials either immediately or after one month (delayed repair). Inflammatory cell count and immunohistochemical analysis of osteopontin-positive area fraction were digitally analysed using the ImageJ software. Delayed furcal perforation repair showed significantly higher inflammatory cell count than immediate repair. No significant difference in inflammatory cell count and immunohistochemical analysis was detected between the three tested materials. The immunohistochemical analysis revealed the highest immunopositive area fraction in the 3-month evaluation period. The experimental tricalcium silicate cement performed similarly to Biodentine and MTA regarding the osteopontin expression during perforation repair, suggesting it is a suitable alternative with favourable handling characters.

Research paper thumbnail of Feline Hypertrophic Osteopathy Associated with Congenital Megaesophagus: Two Case Reports and Literature Review

Journal of the Hellenic Veterinary Medical Society, Oct 15, 2020

Research paper thumbnail of Diagnostic and Treatment Challenges for Diaphragmatic Hernia in Equids: A Concise Review of Literature

Journal of Equine Veterinary Science, Nov 1, 2021

Diaphragmatic hernia (DH) represents an acute abdominal crisis, but respiratory symptoms may be o... more Diaphragmatic hernia (DH) represents an acute abdominal crisis, but respiratory symptoms may be occurring. Different types of diaphragmatic hernias (DHs) like typical DH, Morgagni hernia, hiatal hernia and diaphragmatic diverticulum have been recorded in equids. These DHs have various clinical presentations and prognoses. Early diagnosis and prompt surgical repair are highly recommended in order to diminish the ischemic changes to thoracic and abdominal viscera, thus improving the overall prognosis of equids with DH. Over last decade, the prognosis of DH in equids was better than previously believed due to improvements in diagnostic tools, general anesthesia of equids, (particularly those with systemic compromise) and gastrointestinal surgery, development of thoracoscopic techniques and use of staples for surgical repair. Nevertheless, several diagnostic and treatment challenges are still present during management of DH in equids. Diagnostic challenges include inconsistent clinical signs of DH, acute nature of the associated colic and absence of highly qualified diagnostic tools. Increased awareness of the case, good clinical observation and availability of diagnostic tools are recommended to overcome these challenges. Also anesthesia, reduction of large herniated organs and herniorrhaphy are major challenges. Preoperative stabilization, careful monitoring, selection of the most accessible surgical approach and good intraoperative as well as postoperative support are highly recommended to overcome these challenges. Surgical repair, particularly prosthetic herniorrhaphy seems to be effective to close the diaphragmatic defect and prevent recurrences. The present review describes the different types of DHs in equids, their diagnostic and treatment challenges and how to overcome these challenges.

Research paper thumbnail of Regenerative endodontic therapy in mature teeth with necrotic pulp and apical periodontitis using two disinfection protocols

BMC Oral Health, Mar 22, 2023

Research paper thumbnail of First report of nephrocutaneous fistula in a cat

Bulgarian Journal of Veterinary Medicine, 2022

Research paper thumbnail of Luxation of the patella in foals: A treatment challenge

Equine Veterinary Education, Mar 14, 2021

Research paper thumbnail of Biosealer versus Biodentine for direct pulp capping in dog’s teeth: A histopathological evaluation

Research Square (Research Square), Aug 14, 2019

Research paper thumbnail of MRI features of multiple phalangeal disorders in a draft horse – a case report

Veterinarski arhiv

Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness ... more Magnetic resonance imaging (MRI) is fast becoming the gold standard diagnostic tool for lameness in equines. This case report describes the MRI features of multiple phalangeal disorders in a 9-year-old horse with chronic unilateral forelimb lameness due to severe trauma. A complete case history was taken, and a thorough clinical examination, radiography, ultrasonography and MRI were performed. Radiography and ultrasonography showed limited value. MRI revealed edema of the navicular bursa (NB), distal sesamoidean impar desmitis, DDFT injury at navicular and post-navicular levels, cartilage erosion of the pastern and coffin joints, medial collateral desmitis of the fetlock joint, medial collateral sesamoidean desmitis, and lateral collateral desmitis of the coffin joint. In conclusion, severe trauma may induce multiple phalangeal disorders with severe pain in draft horses, and MRI is superior to other diagnostic imaging modalities, such as radiography and ultrasonography, for diagnosi...

Research paper thumbnail of The impact of membrane perforation and L-PRF for vertical ridge augmentation with a xenogeneic block graft: an experimental study in a canine model

Clinical Oral Investigations

Objectives This study evaluated clinically and histologically the efficacy of modified perforat... more Objectives This study evaluated clinically and histologically the efficacy of modified perforated collagen membrane (PCM) and/or leukocyte- and platelet-rich fibrin (L-PRF) in combination with xenogeneic block bone graft in the vertical alveolar ridge augmentation. Materials and methods Six adult mongrel dogs were enrolled in this randomized blinded study. After defect preparation, xenogeneic screw-fixed block graft was covered by an occlusive collagen membrane in group 1 that represented the control group (Block + CM). In group 2, L-PRF membrane was added first before top coverage by occlusive collagen membrane (Block + L-PRF + CM). Groups 3 (Block + PCM) and 4 (Block + L-PRF + PCM) were identical to the first two groups except that the occlusive collagen membrane was replaced by a perforated one. Following a healing period of 2 months, the dogs were submitted to the surgical reentry phase for clinical and histological evaluation. Results Clinically, no significant differences we...

Research paper thumbnail of The use of Xenogeneic Block Graft and Perforated Collagen Membrane Augmented L-PRF Coverage in Vertical Ridge Augmentation: An Experimental Study in Canine Model

Research Square (Research Square), Jan 19, 2023

Research paper thumbnail of Histopathological and immunohistochemical profiles of pulp tissues in immature dogs’ teeth to two recently introduced pulpotomy materials

Clinical Oral Investigations

Objective The pulpal response to Hoffmann’s Pulpine mineral (PMIN) and Pulpine NE (PNE) was compa... more Objective The pulpal response to Hoffmann’s Pulpine mineral (PMIN) and Pulpine NE (PNE) was compared to mineral trioxide aggregate (MTA) when used as pulpotomy materials in immature permanent teeth in dogs. Materials and methods Immature premolars were randomly divided according to the observation period into three equal groups (n = 24) (10 days, 30 days, and 90 days) then furtherly subdivided into 3 subgroups according to the material used. Histopathological analysis regarding inflammatory cell infiltration and dentin bridge (DB) formation was done. Immunohistochemical analysis was performed using osteopontin marker. Results The results showed that after 90 days, both MTA and PMIN subgroups had 100% complete thick DB without inflammation in 87.5% of the samples, while the PNE subgroup failed to form DB in 37.5% of the samples and 50% of samples showed thin initial DB with heavy inflammation in 62.5% of the samples. There was no significant difference between MTA and PMIN, while the...

Research paper thumbnail of Evaluation of Particulate Autogenous Bone Graft Harvested by Auto Chip Maker versus Milling of Block Bone Graft (Experimental Study with Biochemical and Genetic Assessment)

Research paper thumbnail of Biological Pulp Response of Pulpine, Polyamidoamine Dendrimer and Their Combination in Dogs and their Remineralizing Effect on Carious Affected Human Dentin: A Randomized Clinical Trial

Al-Azhar Dental Journal for Girls, 2021

Purpose: This study was conducted to evaluate the biological pulp response to Pulpine and Polyami... more Purpose: This study was conducted to evaluate the biological pulp response to Pulpine and Polyamidoamine Dendrimer and their combination in dogs and comparing their remineralizing potentials on human caries affected dentin. Materials and Methods: Forty-five teeth in 3 mongrel dogs were randomly divided into 3 groups according to the applied pulp capping material; group A1: Pulpine NE, group A2: PAMAM G3 and group A3: Pulpine and PAMAM. Class V cavities were prepared; the pulps were mechanically exposed and finally restored with resin modified glass ionomer. Histological evaluation was performed after 2 weeks, 1 and 2 months. The in vivo study was designed as a randomized, unicenter, parallel, two arms, double– blind split mouth clinical trial with 1:1 allocation ratio. The subjects who fulfilled the eligibility criteria were asked to participate and signed informed consent. Twenty-eight adhesive occlusal cavities were prepared; infected dentin was removed leaving carious affected de...

Research paper thumbnail of The effect of changing apical foramen diameter on regenerative potential of mature teeth with necrotic pulp and apical periodontitis

Clinical Oral Investigations, 2021

Objective The aim of the present study was to evaluate the regenerative potential of permanent ne... more Objective The aim of the present study was to evaluate the regenerative potential of permanent necrotic mature teeth with different apical foramen diameters in comparison to conventional endodontic treatment. Methods Pulp necrosis and periapical pathosis were induced in premolars (144 roots) in six mongrel dogs. The infected teeth were divided into six groups; conventional root canal treatment (RCT) group prepared up to apical preparation sizes #F3 ProTaper file, regenerative endodontic therapy (RET) groups; groups RET-F3, RET-F4, and RET-F5 where regenerative endodontic procedures were applied after apical preparation up to sizes #F3, F4, and F5 ProTaper file, respectively, control groups; and positive (P) and negative (N) groups included infected and healthy teeth respectively. Each group (12 teeth with 24 roots each) was further subdivided into two subgroups (6 teeth with 12 roots each) according to the evaluation periods 1 and 3 months; each group was equally randomized in the upper and lower jaws during each evaluation period. Results The inflammatory cell counts for the RCT group were statistically significantly higher than that of all RET groups at the first period and only significantly higher than RET-F5 at the second period. The enlargement of the apical foramen to #F5 showed no statistically significant difference with #F4 regarding the inflammatory cell count, tissue in-growth inside the root canal, and percentage of bone resorption. The tissue formed after RET was not true pulp tissue and is considered tissue repair with connective tissue, dilated blood vessels, and cementum-like structures. Conclusion Increasing the apical size diameter was associated with less inflammatory cell count, less percentage of bone resorption, more tissue ingrowth, and more percentage of new hard tissue formation inside root canal. The minimum apical diameter that should be enlarged for RET is 0.4 mm. Clinical relevance RET could be used as an alternative procedure to RCT for the treatment of mature teeth with necrotic pulp and apical periodontitis.