lobna abdelsalam | Cairo University (original) (raw)

Papers by lobna abdelsalam

Public spaces are the structural elements of any architectural environment, areas of socialcohesi... more Public spaces are the structural elements of any architectural environment, areas of socialcohesion, spaces of coexistence, outbreaks of urbanity programmatically designed to attract all typesof public, to reunite the citizens of the city and to improve the dynamics of the urban space; in oneword, the city’s front window. The quality of urban space is primarily determined by the quality of thepublic areas belonging to the city, the areas that the city offers for common use to its inhabitants. Theaesthetic of landscape improvements have benefic influences on the cultural and artistic education ofcitizens and on their moral well-being. This study aims to establish the aesthetic values generated bythe structural elements of landscape improvements and their influences on human perception. Once theaesthetical values being identified, the paper tries to establish parameters for measuring the quality ofpublic space, and in the end, aims to generate hypothesis whose final goal is to incre...

ECS Meeting Abstracts, 2014

Gene Reports, 2020

Abstract Background Hemophilia A (HA) is an X-linked bleeding disorder caused by the absence or r... more Abstract Background Hemophilia A (HA) is an X-linked bleeding disorder caused by the absence or reduced activity of coagulation factor VIII. Inversion of intron 22 (inv22) is detected in 50% of cases of severe HA and is known as a major risk factor for inhibitor formation. Aim The aim was to study inv22 prevalence among the Egyptian hemophilic children and the genetic association of inv22 with the disease severity and inhibitor formation. We also aimed to implement a faster and more accurate molecular technology to facilitate inv22 detection in the genetic diagnosis of hemophilic patients. Methods This is a cross sectional study performed on 60 hemophilic boys. DNA samples were tested for inv22 by coupled long distance polymerase chain reaction with Syber green detection system using Real Time PCR technology. Results Out of the 60 hemophilic boys, 24 patients harbored inv22. All of them presented with severe HA, where allof them had joint bleeds [OR (95% CI): 1.9 (1.5–2.5); p 0.008] Inv 22 was not detected in patients having mild or moderate HA [OR (95% CI): 2.5 (1.71 – –3.65); p 0.001]. . Moreover, there was no significant correlation between the occurrence of inv. 22 and inhibitor formation (p = 0.5). Conclusion There is a significant direct correlation between Inv22 and the severity of the disease, however, there was no significant correlation between Inv22 occurrence and inhibitor formation. Our modified PCR protocol that couples LD-PCR with Syber green detection system using Real Time PCR technology is more rapid and convenient for Inv22 detection in genetic diagnosis of HA. It could be applied clinically in the Pre-genetic diagnosis in the future.

Arab Journal of Gastroenterology, 2019

Open Forum Infectious Diseases, 2015

Journal of Infection, 2015

Objectives: The Centers for Disease Control and Prevention (CDC) only recommends risk-based HCV s... more Objectives: The Centers for Disease Control and Prevention (CDC) only recommends risk-based HCV screening for pregnant women in the United States. This study sought to determine the reliability of risk-based versus universal HCV screening for pregnant women in Egypt, a country with the world's highest HCV prevalence that also relies on risk-based screening, and to identify additional characteristics that could increase the reliability of risk-based screening. Methods: Pregnant women attending the Cairo University antenatal clinic were tested for anti-HCV antibodies and RNA, and demographic characteristics and risk factors for infection were assessed. Results: All 1250 pregnant women approached agreed to participate (100%) with a mean age of 27.4 AE 5.5 years (range:16e45). HCV antibodies and RNA were positive in 52 (4.2%) and 30 (2.4%) women respectively. After adjustment, only age (OR:1.08, 95%CI:1.002e1.16,

Infectious Agents and Cancer, 2016

Background: The association of human papillomavirus (HPV) with cervical cancer is well establishe... more Background: The association of human papillomavirus (HPV) with cervical cancer is well established. Aim: To investigate HPV genotype distribution and co-infection occurrence in cervical specimens from a group of Egyptian women. Methods: A group of 152 women with and without cervical lesions were studied. All women had cervical cytology and HPV testing. They were classified according to cytology into those with normal cytology, with squamous intraepithelial lesions (SIL) and invasive squamous cell carcinoma (SCC). Cervical samples were analyzed to identify the presence of HPV by PCR, and all positive HPV-DNA samples underwent viral genotype analysis by means of LINEAR ARRAY HPV Genotyping assay. Results: A total of 26 HPV types with a prevalence of 40.8 % were detected. This prevalence was distributed as follows: 17.7 % among cytologically normal females, 56.5, 3.2, and 22.6 % among those with LSIL, HSIL and invasive SCC respectively. Low-risk HPV types were detected in 81.8 % of the cytologically-normal women, in 5.7 % of those in LSIL women, and in 14.3 % of infections with invasive SCC, while no low-risk types were detected in HSIL. High-risk HPV types were detected in 18.2 % of infections in the cytologically normal women, 14.3 % of infections in LSIL, and in 21.4 % of invasive lesions. The probable and possible carcinogenic HPV were not detected as single infections. Mixed infection was present in 80 % of women with LSIL, in 100 % of those with HSIL, and in 64.3 % of those with invasive SCC. This difference was statistically significant. HPV 16, 18 and 31 were the most prevalent HR HPV types, constituting 41.9, 29.03 and 12.9 % respectively, and HPV 6, 62 and CP6108 were the most prevalent LR HPV types constituting 11.3, 9.7 and 9.7 % respectively. Conclusion: These data expand the knowledge concerning HPV prevalence and type distribution in Egypt which may help to create a national HPV prevention program. HPV testing using the LINEAR ARRAY HPV Genotyping assay is a useful tool when combined with cytology in the diagnosis of mixed and non-conventional HPV viral types.

Surgical Atlas of Cleft Palate and Palatal Fistulae, 2022

Arab Journal of Gastroenterology

Zagazig University Medical Journal, Sep 1, 2013

Background: Although current improvements in surgery, chemotherapy and radiotherapy, the still su... more Background: Although current improvements in surgery, chemotherapy and radiotherapy, the still survival of astrocytoma's patient is poor. It is assumed that the combined expression of BMI1 and EZH2 may be associated with malignant transformation of astrocytomas and also it may reveal the biological aggressiveness of this disease. Aim of work: assessment the value of the combined immunohistochemical expression of BMI1 and EZH2 and their correlation with the clinicopathological characters and prognosis in astrocytoma patient. Subjects & Methods: BMI1 and EZH2 expressions were evaluated using immunohistochemical staining in 70 patients 40 cases with astrocytomas and 30 cases of non-neoplastic brain tissue. The relationship between their expressions and clinicopathological factors were analyzed. Results: A significant difference (P<0.002) between expression of BMI1 and EZH2 in astrocytoma compared to corresponding non-neoplastic brain tissue. A significant association was found between high expression of BMI1 and EZH2 and WHO high grades in astrocytomas. No statistically significant association was found between BMI1 or EZH2 with gender of patients, age at diagnosis, site and size of tumor (P > 0.05). The spearman correlation analysis revealed a significant positive association between BMI1 and EZH2 expressions (r = 0.311; P=0.05) revealing direct relationship between BMI1 and EZH2. Conclusions: BMI1 and EZH2 were involved in astrocytoma malignant transformation and poor prognosis in astrocytoma particularly glioblastoma.

Open Forum Infectious Diseases, 2015

Arab Journal of Gastroenterology

Archives of Medical Science - Civilization Diseases, 2017

Introduction: Autophagy was found to play a major role in the pathogenesis of acute lymphoblastic... more Introduction: Autophagy was found to play a major role in the pathogenesis of acute lymphoblastic leukemia (ALL). In this study we investigated the expression of beclin-1, Bad, Bax, Bcl-2, and Bcl-xL in patients with ALL. Material and methods: This was a comparative study conducted on 100 ALL patients (age 8-15) divided into 2 groups. The first group, the ALL group, comprised ALL cases at their initial diagnosis (46 patients), while the second group, the Remission group, comprised in-remission cases (50 patients). mRNA expression levels in patients' blood samples were determined using real-time polymerase chain reaction (PCR). Results: Beclin-1 levels were significantly lower in the ALL group than in the Remission group (0.22 ±0.03 vs. 196.8 ±32.47; p = 0.001). Bad levels were significantly lower in the ALL group (1.0 ±0.18 vs. 163.6 ±36.2; p = 0.001), while Bax levels were significantly higher in the ALL group than in the Remission group (131.52 ±31.4 vs. 4.29 ±0.64; p = 0.001). Bcl-2 levels were significantly higher in the ALL group (2678.91 ±575.5 vs. 7.56 ±2.9; p = 0.001), and Bcl-xL levels were also significantly higher in the ALL group (142.99 ±24.43 vs. 0.99 ±0.2; p = 0.001). There was negative correlation between immunophenotyping with beclin-1 (r =-0.725; p < 0.001), while there was a positive correlation with Bcl-2 (r = 0.533; p < 0.001). Conclusions: Our findings reveal potential prognostic value for these markers in pediatric ALL, with regard to the delicate mutual balance among them.

Archives of Medical Science, 2019

Introduction: New diagnostic tools are needed to accurately detect acute myocardial infarction (A... more Introduction: New diagnostic tools are needed to accurately detect acute myocardial infarction (AMI) in patients with end stage renal disease (ESRD) presenting with ischemic chest pain. We aimed in this study to investigate circulating miR-122,-192 and-499 expression levels in patients with AMI on top of ESRD and evaluate the potential of these miRNAs as blood-based biomarkers for AMI in patients with ESRD. Material and methods: The study included 80 ESRD patients without AMI, 80 patients with ESRD associated with AMI and 60 healthy subjects. Assessment of microRNAs was done using SYBR Green based real-time PCR. Results: Levels of miR-122 were 28-fold and 20-fold higher in controls than in ESRD patients with or without AMI respectively (p < 0.001), while no differences were detected between the two patient groups (p = 0.9). Levels of miR-192 showed a marked increase in ESRD patients with and without AMI compared to the control group (> 500-fold, > 8000-fold respectively, p ≤ 0.001). Patients who developed AMI had lower expression than ESRD patients without AMI (p < 0.001). Non-significant miR-499 elevation was found in ESRD patients without cardiac disease compared to the control group, while highly significant elevation of miR-499 was demonstrated in ESRD patients who developed AMI compared to other ESRD patients and the control group (> 100-fold, > 350-fold respectively, p = 0.001). Conclusions: Altered expression of miR-122 and-192 may contribute in pathogenesis of ESRD. MiR-192 and-499 may serve as potential biomarkers for AMI in ESRD. Further studies are needed to correlate these miRNAs with disease progression and outcome.

The Turkish Journal of Gastroenterology

European Geriatric Medicine

Clinical Infectious Diseases

Background. Postpartum hepatitis C viral (HCV) load decline followed by spontaneous clearance has... more Background. Postpartum hepatitis C viral (HCV) load decline followed by spontaneous clearance has been previously described. Herein we identify predictors for viral decline in a cohort of HCV-infected postpartum women. Methods. Pregnant women at Cairo University were screened for anti-HCV antibodies and HCV RNA, and viremic women were tested for quantitative HCV RNA at 3, 6, 9, and 12 months postpartum. Spontaneous clearance was defined as undetectable viremia twice at least 6-months apart. Associations between viral load and demographic, obstetrical, HCV risk factors, and interleukin-28B gene (IL28B) polymorphism (rs12979860) were assessed. Results. Of 2514 women, 97 (3.9%) had anti-HCV antibodies, 54 (2.1%) were viremic and of those, 52 (2.1%) agreed to IL28B testing. From pregnancy until 12 months postpartum, IL28B-CC allele women had a significant viral decline (P = .009). After adjusting, the IL28B-CC allele had a near significant difference compared to the CT allele (odds ratio [OR], 0.75; 95% confidence interval [CI], 0.75,1.00; P = .05), but not the TT allele (OR, 0.91; 95% CI, 0.61,1.38; P = .64). All 14/52 (26.9%) women who subsequently cleared were among the 15 with undetectable viremia at 12 months, making that time point a strong predictor of subsequent clearance (sensitivity = 100%, specificity = 97.4%, positive predictive value = 93.3%, negative predictive value = 100%). Conclusions. In this HCV-infected cohort of postpartum women 26.9% spontaneously cleared. IL28B-CC genotype and 12-month postpartum undetectable viremia were the best predictors for viral decline and subsequent clearance. These 2 predictors should influence clinical decision making.

Public spaces are the structural elements of any architectural environment, areas of socialcohesi... more Public spaces are the structural elements of any architectural environment, areas of socialcohesion, spaces of coexistence, outbreaks of urbanity programmatically designed to attract all typesof public, to reunite the citizens of the city and to improve the dynamics of the urban space; in oneword, the city’s front window. The quality of urban space is primarily determined by the quality of thepublic areas belonging to the city, the areas that the city offers for common use to its inhabitants. Theaesthetic of landscape improvements have benefic influences on the cultural and artistic education ofcitizens and on their moral well-being. This study aims to establish the aesthetic values generated bythe structural elements of landscape improvements and their influences on human perception. Once theaesthetical values being identified, the paper tries to establish parameters for measuring the quality ofpublic space, and in the end, aims to generate hypothesis whose final goal is to incre...

ECS Meeting Abstracts, 2014

Gene Reports, 2020

Abstract Background Hemophilia A (HA) is an X-linked bleeding disorder caused by the absence or r... more Abstract Background Hemophilia A (HA) is an X-linked bleeding disorder caused by the absence or reduced activity of coagulation factor VIII. Inversion of intron 22 (inv22) is detected in 50% of cases of severe HA and is known as a major risk factor for inhibitor formation. Aim The aim was to study inv22 prevalence among the Egyptian hemophilic children and the genetic association of inv22 with the disease severity and inhibitor formation. We also aimed to implement a faster and more accurate molecular technology to facilitate inv22 detection in the genetic diagnosis of hemophilic patients. Methods This is a cross sectional study performed on 60 hemophilic boys. DNA samples were tested for inv22 by coupled long distance polymerase chain reaction with Syber green detection system using Real Time PCR technology. Results Out of the 60 hemophilic boys, 24 patients harbored inv22. All of them presented with severe HA, where allof them had joint bleeds [OR (95% CI): 1.9 (1.5–2.5); p 0.008] Inv 22 was not detected in patients having mild or moderate HA [OR (95% CI): 2.5 (1.71 – –3.65); p 0.001]. . Moreover, there was no significant correlation between the occurrence of inv. 22 and inhibitor formation (p = 0.5). Conclusion There is a significant direct correlation between Inv22 and the severity of the disease, however, there was no significant correlation between Inv22 occurrence and inhibitor formation. Our modified PCR protocol that couples LD-PCR with Syber green detection system using Real Time PCR technology is more rapid and convenient for Inv22 detection in genetic diagnosis of HA. It could be applied clinically in the Pre-genetic diagnosis in the future.

Arab Journal of Gastroenterology, 2019

Open Forum Infectious Diseases, 2015

Journal of Infection, 2015

Objectives: The Centers for Disease Control and Prevention (CDC) only recommends risk-based HCV s... more Objectives: The Centers for Disease Control and Prevention (CDC) only recommends risk-based HCV screening for pregnant women in the United States. This study sought to determine the reliability of risk-based versus universal HCV screening for pregnant women in Egypt, a country with the world's highest HCV prevalence that also relies on risk-based screening, and to identify additional characteristics that could increase the reliability of risk-based screening. Methods: Pregnant women attending the Cairo University antenatal clinic were tested for anti-HCV antibodies and RNA, and demographic characteristics and risk factors for infection were assessed. Results: All 1250 pregnant women approached agreed to participate (100%) with a mean age of 27.4 AE 5.5 years (range:16e45). HCV antibodies and RNA were positive in 52 (4.2%) and 30 (2.4%) women respectively. After adjustment, only age (OR:1.08, 95%CI:1.002e1.16,

Infectious Agents and Cancer, 2016

Background: The association of human papillomavirus (HPV) with cervical cancer is well establishe... more Background: The association of human papillomavirus (HPV) with cervical cancer is well established. Aim: To investigate HPV genotype distribution and co-infection occurrence in cervical specimens from a group of Egyptian women. Methods: A group of 152 women with and without cervical lesions were studied. All women had cervical cytology and HPV testing. They were classified according to cytology into those with normal cytology, with squamous intraepithelial lesions (SIL) and invasive squamous cell carcinoma (SCC). Cervical samples were analyzed to identify the presence of HPV by PCR, and all positive HPV-DNA samples underwent viral genotype analysis by means of LINEAR ARRAY HPV Genotyping assay. Results: A total of 26 HPV types with a prevalence of 40.8 % were detected. This prevalence was distributed as follows: 17.7 % among cytologically normal females, 56.5, 3.2, and 22.6 % among those with LSIL, HSIL and invasive SCC respectively. Low-risk HPV types were detected in 81.8 % of the cytologically-normal women, in 5.7 % of those in LSIL women, and in 14.3 % of infections with invasive SCC, while no low-risk types were detected in HSIL. High-risk HPV types were detected in 18.2 % of infections in the cytologically normal women, 14.3 % of infections in LSIL, and in 21.4 % of invasive lesions. The probable and possible carcinogenic HPV were not detected as single infections. Mixed infection was present in 80 % of women with LSIL, in 100 % of those with HSIL, and in 64.3 % of those with invasive SCC. This difference was statistically significant. HPV 16, 18 and 31 were the most prevalent HR HPV types, constituting 41.9, 29.03 and 12.9 % respectively, and HPV 6, 62 and CP6108 were the most prevalent LR HPV types constituting 11.3, 9.7 and 9.7 % respectively. Conclusion: These data expand the knowledge concerning HPV prevalence and type distribution in Egypt which may help to create a national HPV prevention program. HPV testing using the LINEAR ARRAY HPV Genotyping assay is a useful tool when combined with cytology in the diagnosis of mixed and non-conventional HPV viral types.

Surgical Atlas of Cleft Palate and Palatal Fistulae, 2022

Arab Journal of Gastroenterology

Zagazig University Medical Journal, Sep 1, 2013

Background: Although current improvements in surgery, chemotherapy and radiotherapy, the still su... more Background: Although current improvements in surgery, chemotherapy and radiotherapy, the still survival of astrocytoma's patient is poor. It is assumed that the combined expression of BMI1 and EZH2 may be associated with malignant transformation of astrocytomas and also it may reveal the biological aggressiveness of this disease. Aim of work: assessment the value of the combined immunohistochemical expression of BMI1 and EZH2 and their correlation with the clinicopathological characters and prognosis in astrocytoma patient. Subjects & Methods: BMI1 and EZH2 expressions were evaluated using immunohistochemical staining in 70 patients 40 cases with astrocytomas and 30 cases of non-neoplastic brain tissue. The relationship between their expressions and clinicopathological factors were analyzed. Results: A significant difference (P<0.002) between expression of BMI1 and EZH2 in astrocytoma compared to corresponding non-neoplastic brain tissue. A significant association was found between high expression of BMI1 and EZH2 and WHO high grades in astrocytomas. No statistically significant association was found between BMI1 or EZH2 with gender of patients, age at diagnosis, site and size of tumor (P > 0.05). The spearman correlation analysis revealed a significant positive association between BMI1 and EZH2 expressions (r = 0.311; P=0.05) revealing direct relationship between BMI1 and EZH2. Conclusions: BMI1 and EZH2 were involved in astrocytoma malignant transformation and poor prognosis in astrocytoma particularly glioblastoma.

Open Forum Infectious Diseases, 2015

Arab Journal of Gastroenterology

Archives of Medical Science - Civilization Diseases, 2017

Introduction: Autophagy was found to play a major role in the pathogenesis of acute lymphoblastic... more Introduction: Autophagy was found to play a major role in the pathogenesis of acute lymphoblastic leukemia (ALL). In this study we investigated the expression of beclin-1, Bad, Bax, Bcl-2, and Bcl-xL in patients with ALL. Material and methods: This was a comparative study conducted on 100 ALL patients (age 8-15) divided into 2 groups. The first group, the ALL group, comprised ALL cases at their initial diagnosis (46 patients), while the second group, the Remission group, comprised in-remission cases (50 patients). mRNA expression levels in patients' blood samples were determined using real-time polymerase chain reaction (PCR). Results: Beclin-1 levels were significantly lower in the ALL group than in the Remission group (0.22 ±0.03 vs. 196.8 ±32.47; p = 0.001). Bad levels were significantly lower in the ALL group (1.0 ±0.18 vs. 163.6 ±36.2; p = 0.001), while Bax levels were significantly higher in the ALL group than in the Remission group (131.52 ±31.4 vs. 4.29 ±0.64; p = 0.001). Bcl-2 levels were significantly higher in the ALL group (2678.91 ±575.5 vs. 7.56 ±2.9; p = 0.001), and Bcl-xL levels were also significantly higher in the ALL group (142.99 ±24.43 vs. 0.99 ±0.2; p = 0.001). There was negative correlation between immunophenotyping with beclin-1 (r =-0.725; p < 0.001), while there was a positive correlation with Bcl-2 (r = 0.533; p < 0.001). Conclusions: Our findings reveal potential prognostic value for these markers in pediatric ALL, with regard to the delicate mutual balance among them.

Archives of Medical Science, 2019

Introduction: New diagnostic tools are needed to accurately detect acute myocardial infarction (A... more Introduction: New diagnostic tools are needed to accurately detect acute myocardial infarction (AMI) in patients with end stage renal disease (ESRD) presenting with ischemic chest pain. We aimed in this study to investigate circulating miR-122,-192 and-499 expression levels in patients with AMI on top of ESRD and evaluate the potential of these miRNAs as blood-based biomarkers for AMI in patients with ESRD. Material and methods: The study included 80 ESRD patients without AMI, 80 patients with ESRD associated with AMI and 60 healthy subjects. Assessment of microRNAs was done using SYBR Green based real-time PCR. Results: Levels of miR-122 were 28-fold and 20-fold higher in controls than in ESRD patients with or without AMI respectively (p < 0.001), while no differences were detected between the two patient groups (p = 0.9). Levels of miR-192 showed a marked increase in ESRD patients with and without AMI compared to the control group (> 500-fold, > 8000-fold respectively, p ≤ 0.001). Patients who developed AMI had lower expression than ESRD patients without AMI (p < 0.001). Non-significant miR-499 elevation was found in ESRD patients without cardiac disease compared to the control group, while highly significant elevation of miR-499 was demonstrated in ESRD patients who developed AMI compared to other ESRD patients and the control group (> 100-fold, > 350-fold respectively, p = 0.001). Conclusions: Altered expression of miR-122 and-192 may contribute in pathogenesis of ESRD. MiR-192 and-499 may serve as potential biomarkers for AMI in ESRD. Further studies are needed to correlate these miRNAs with disease progression and outcome.

The Turkish Journal of Gastroenterology

European Geriatric Medicine

Clinical Infectious Diseases

Background. Postpartum hepatitis C viral (HCV) load decline followed by spontaneous clearance has... more Background. Postpartum hepatitis C viral (HCV) load decline followed by spontaneous clearance has been previously described. Herein we identify predictors for viral decline in a cohort of HCV-infected postpartum women. Methods. Pregnant women at Cairo University were screened for anti-HCV antibodies and HCV RNA, and viremic women were tested for quantitative HCV RNA at 3, 6, 9, and 12 months postpartum. Spontaneous clearance was defined as undetectable viremia twice at least 6-months apart. Associations between viral load and demographic, obstetrical, HCV risk factors, and interleukin-28B gene (IL28B) polymorphism (rs12979860) were assessed. Results. Of 2514 women, 97 (3.9%) had anti-HCV antibodies, 54 (2.1%) were viremic and of those, 52 (2.1%) agreed to IL28B testing. From pregnancy until 12 months postpartum, IL28B-CC allele women had a significant viral decline (P = .009). After adjusting, the IL28B-CC allele had a near significant difference compared to the CT allele (odds ratio [OR], 0.75; 95% confidence interval [CI], 0.75,1.00; P = .05), but not the TT allele (OR, 0.91; 95% CI, 0.61,1.38; P = .64). All 14/52 (26.9%) women who subsequently cleared were among the 15 with undetectable viremia at 12 months, making that time point a strong predictor of subsequent clearance (sensitivity = 100%, specificity = 97.4%, positive predictive value = 93.3%, negative predictive value = 100%). Conclusions. In this HCV-infected cohort of postpartum women 26.9% spontaneously cleared. IL28B-CC genotype and 12-month postpartum undetectable viremia were the best predictors for viral decline and subsequent clearance. These 2 predictors should influence clinical decision making.