Robert E. Fields | University of Cambridge (original) (raw)

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Research paper thumbnail of The measurement of amino groups in proteins and peptides

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of Active site of human liver aldehyde dehydrogenase

Biochemistry, Jan 1, 1987

Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde ... more Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.

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Research paper thumbnail of Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2, 4-dinitrophenylhydrazine

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of A spectrophotometric method for the microdetermination of periodate

Biochemical Journal, Jan 1, 1968

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Research paper thumbnail of Purification of ribonuclease T1 by diethylaminoethylcellulose chromatography

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of Recombinant rat and murine immune interferons are phosphorylated at a single site, Ser132

Journal of interferon …, Jan 1, 1988

Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylat... more Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylated by bovine heart muscle cAMP-dependent protein kinase at a single site, in contrast to human (Hu) IFN-gamma, which was reported to be phosphorylated at two different serine residues. Chromatography of a Staphylococcal aureus V8 protease digest of Ra or MuIFN-gamma indicated that the site of phosphorylation was in the carboxy-terminal undecamer fragment of the protein. Due to inherent problems in measuring both phenylthiohydantoin-serine (PTH-serine) and PTH-phosphoserine with an automated sequenator, a novel approach, involving partial Edman degradation of aliquots of the peptide followed by high performance liquid chromatography (HPLC) analysis, was developed. It was determined that Ser132 is the exclusive site of phosphorylation for both IFNs.

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Research paper thumbnail of Peptides mimicking selected disulfide loops in HIV-1 gp120, other than V3, do not elicit virus-neutralizing antibodies

AIDS research and …, Jan 1, 1991

... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not ... more ... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not Elicit Virus-Neutralizing Antibodies A. ROBERT NEURATH, NATHAN STRICK, ROBERT FIELDS, and SHIBO JIANG ABSTRACT The ...

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Research paper thumbnail of The measurement of amino groups in proteins and peptides

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of Active site of human liver aldehyde dehydrogenase

Biochemistry, Jan 1, 1987

Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde ... more Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Micro method for determination of reactive carbonyl groups in proteins and peptides, using 2, 4-dinitrophenylhydrazine

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of A spectrophotometric method for the microdetermination of periodate

Biochemical Journal, Jan 1, 1968

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Research paper thumbnail of Purification of ribonuclease T1 by diethylaminoethylcellulose chromatography

Biochemical Journal, Jan 1, 1971

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Research paper thumbnail of Recombinant rat and murine immune interferons are phosphorylated at a single site, Ser132

Journal of interferon …, Jan 1, 1988

Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylat... more Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylated by bovine heart muscle cAMP-dependent protein kinase at a single site, in contrast to human (Hu) IFN-gamma, which was reported to be phosphorylated at two different serine residues. Chromatography of a Staphylococcal aureus V8 protease digest of Ra or MuIFN-gamma indicated that the site of phosphorylation was in the carboxy-terminal undecamer fragment of the protein. Due to inherent problems in measuring both phenylthiohydantoin-serine (PTH-serine) and PTH-phosphoserine with an automated sequenator, a novel approach, involving partial Edman degradation of aliquots of the peptide followed by high performance liquid chromatography (HPLC) analysis, was developed. It was determined that Ser132 is the exclusive site of phosphorylation for both IFNs.

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Research paper thumbnail of Peptides mimicking selected disulfide loops in HIV-1 gp120, other than V3, do not elicit virus-neutralizing antibodies

AIDS research and …, Jan 1, 1991

... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not ... more ... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not Elicit Virus-Neutralizing Antibodies A. ROBERT NEURATH, NATHAN STRICK, ROBERT FIELDS, and SHIBO JIANG ABSTRACT The ...

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