Robert E. Fields | University of Cambridge (original) (raw)
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Papers by Robert E. Fields
Biochemical Journal, Jan 1, 1971
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Biochemistry, Jan 1, 1987
Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde ... more Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.
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Biochemical Journal, Jan 1, 1971
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Biochemical Journal, Jan 1, 1968
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Biochemical Journal, Jan 1, 1971
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Journal of interferon …, Jan 1, 1988
Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylat... more Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylated by bovine heart muscle cAMP-dependent protein kinase at a single site, in contrast to human (Hu) IFN-gamma, which was reported to be phosphorylated at two different serine residues. Chromatography of a Staphylococcal aureus V8 protease digest of Ra or MuIFN-gamma indicated that the site of phosphorylation was in the carboxy-terminal undecamer fragment of the protein. Due to inherent problems in measuring both phenylthiohydantoin-serine (PTH-serine) and PTH-phosphoserine with an automated sequenator, a novel approach, involving partial Edman degradation of aliquots of the peptide followed by high performance liquid chromatography (HPLC) analysis, was developed. It was determined that Ser132 is the exclusive site of phosphorylation for both IFNs.
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AIDS research and …, Jan 1, 1991
... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not ... more ... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not Elicit Virus-Neutralizing Antibodies A. ROBERT NEURATH, NATHAN STRICK, ROBERT FIELDS, and SHIBO JIANG ABSTRACT The ...
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Biochemical Journal, Jan 1, 1971
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Biochemistry, Jan 1, 1987
Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde ... more Bromoacetophenone (2-bromo-1-phenylethanone) functions as an affinity reagent for human aldehyde dehydrogenase (EC 1.2.1.3) and has been found specifically to label a unique tryptic peptide in the enzyme. Amino-terminal sequence analysis of the labeled peptide after purification by two different procedures revealed the following sequence: Val-Thr-Leu-Glu-Leu-Gly-Gly-Lys. Radioactivity was found to be associated with the glutamate residue, which was identified as Glu-268 by reference to the known amino acid sequence. This paper constitutes the first identification of an active site of aldehyde dehydrogenase.
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Biochemical Journal, Jan 1, 1971
Bookmarks Related papers MentionsView impact
Biochemical Journal, Jan 1, 1968
Bookmarks Related papers MentionsView impact
Biochemical Journal, Jan 1, 1971
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Journal of interferon …, Jan 1, 1988
Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylat... more Recombinant rat (Ra) and murine (Mu) immune interferons (IFN-gamma) were found to be phosphorylated by bovine heart muscle cAMP-dependent protein kinase at a single site, in contrast to human (Hu) IFN-gamma, which was reported to be phosphorylated at two different serine residues. Chromatography of a Staphylococcal aureus V8 protease digest of Ra or MuIFN-gamma indicated that the site of phosphorylation was in the carboxy-terminal undecamer fragment of the protein. Due to inherent problems in measuring both phenylthiohydantoin-serine (PTH-serine) and PTH-phosphoserine with an automated sequenator, a novel approach, involving partial Edman degradation of aliquots of the peptide followed by high performance liquid chromatography (HPLC) analysis, was developed. It was determined that Ser132 is the exclusive site of phosphorylation for both IFNs.
Bookmarks Related papers MentionsView impact
AIDS research and …, Jan 1, 1991
... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not ... more ... Publishers Peptides Mimicking Selected Disulfide Loops in HIV-1 gpl20, Other Than V3, Do Not Elicit Virus-Neutralizing Antibodies A. ROBERT NEURATH, NATHAN STRICK, ROBERT FIELDS, and SHIBO JIANG ABSTRACT The ...
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