Lipa Deb | Central Agricultural University (CAU), Imphal (original) (raw)

Papers by Lipa Deb

Frontiers in Agronomy

Biological control of plant pathogens has become increasingly possible with the use of fungi, whi... more Biological control of plant pathogens has become increasingly possible with the use of fungi, which have a high reproductive rate (both sexually and asexually) and a short generation time and are very specific to their target. Trichoderma species are found in diverse habitats and experience various interactions with other organisms. They are used as bio-fungicides owing to their plant-protecting abilities, and they produce a large number of secondary metabolites (SMs) accompanied by enrichment in secondary metabolism-associated genes. This article aims to review and discuss the SMs produced by Trichoderma species, including their physiology, mode of action, mass production, and industrial and field applications for the control of plant diseases. We also discuss the evolutionary history, taxonomical gradient, classification, and ecology of Trichoderma species, as well as indirect and direct mechanisms used as plant protectors with gene improvement strategies. Aside from the bioactivi...

International Journal of Current Microbiology and Applied Sciences, 2020

The Pharma Innovation, 2020

The Pharma Innovation, 2021

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. Mechanisms of plant disease suppression by Beauveria sp. involve antibiosis, mycoparasitism, competition, endophytism and induced systemic resistance. Moreover, production of wide array of volatile organic compounds, hydrolytic enzymes (chitinases, amylases, lipases, cellulases, caseinases and proteases) and various secondary metabolites (beauvericin, bassinolide, beauvolide, beauviroloide, oosporein, bassianin and tenellin) triggers its antifungal and antimicrobial properties against various plant pathogens. Beauveria sp. have been reported to suppress diseases caused by various foliar and soil borne phytopathogens belonging to Oomycetes (Phytopthora infestans, Pythium myriotylum, P. debaryanum, P. irregular and P. ultimum ), fungi...

Research Article, 2021

Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has drawn attention worldwide a... more Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has drawn attention worldwide as potential biocontrol agent against insect-pests since decades. In the rice ecosystem, B. bassiana has been established as potential mycoinsecticide against major insect-pests of rice viz., Cnaphalocrocis medunalis, Nilaparvata lugens, Dicladispa armigera, Scirpophaga incertulas etc., but its efficacy against phytopathogens of rice in simultaneous application is least studied. Among major phytopathogens of rice, bacterial leaf blight incurs a yield loss of up to 12-75%, affecting crop at seedling, tillering and vegetative stages. Therefore, recent studies have inclined towards unravelling antimicrobial potential of B. bassiana as effective plant disease antagonist, thereby, opening a newer dimension in dualpurpose crop protection strategies. In the present study, antagonistic abilities of fifteen (15) native isolates of B. bassiana were evaluated against Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice by conducting agar-plug diffusion and inverted plate assays. The results showed that, majority of B. bassiana isolates viz., Bb31, Bb45, Bb48, Bb25, Bb53 were able to inhibit bacterial growth, with maximum per cent inhibition was recorded in the range of 60-84% through development of inhibition zone. Further, antibacterial ability of B. bassiana was also attributed to release of non-volatile inhibitory metabolites responsible for the formation of inhibition zone on the bacterial lawn. These findings provide substantial evidences on antibacterial abilities of B. bassiana against X. oryzae pv. oryzae and further provides a scope to test the efficacy of B. bassiana against bacterial leaf blight of rice in the glasshouse and field condition in addition to their potential entomogenous behaviour against insect-pests of rice.

Phytopathology, 2020

Peach, (Prunus persica (L) Batsch.) is one of the most important stone fruit commercially grown i... more Peach, (Prunus persica (L) Batsch.) is one of the most important stone fruit commercially grown in the hills including Meghalaya. During Feb-June, 2020 in a roving survey we have found infection on twigs and leaves of peach at Umiam and Pepbah of Ribhoi and East Khasi Hills district of Meghalaya. The infected part showing symptoms of twig blight, blossom blight, cankers and fruit rot. Samples of young and matured leaf, blossom, twigs etc with infection were collected. We studied the detailed symptomatology, isolated and purified the associated causal organism. Through Koch’s postulate we confirmed the pathogenic association of the causal agent. Further through cultural, morphological, and micrometry study the microorganism was identified as Monilinia fructicola (G. Winter) Honey and this is the first report from the Meghalaya of North East India.

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. The compatibility of Beauveria isolate BP1.1 was evaluated with different fungicides namely Carbendazim (50 WP) and Copper Oxychloride (50 WP) and botanicals such as Aqueous Neem Leaf Extract (ANLE) (1% w/v), Aqueous Garlic Extract (AGE) (1% w/v) and Neem Seed Kernel Extract (NLKE) (5% w/v) at three different concentrations. The isolate of Beauveria BP1.1 showed maximum growth in Copper Oxychloride (80.50 mm) followed by Neem Leaf Extract (74.75 mm) and Neem Seed kernel Extract (70.75 mm), whereas, the least growth was observed in Garlic extract (60.50 mm) and total inhibition was observed in carbendazim.

Indian Phytopathology, 2021

Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has widely drawn attention of c... more Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has widely drawn attention of crop protection practitioners as potential biocontrol agent against insect-pests since decades. In the present study, antagonistic potential of 22 native of B. bassiana isolates were evaluated against damping-off disease of tomato caused by Pythium sp. Results showed that all B. bassiana isolates were able to inhibit mycelial growth of P. myriotylum to the extent of 68–82%. Further studies were conducted to understand the ability of screened B. bassiana isolates to produce cell wall degrading enzymes (CWDEs) viz., amylase, caesinase, chitinase, cellulase, lipase and protease respectively. The potential B. bassiana isolates showed dynamic colonization efficiency in tomato plants with colonization percentage (%) as high as 72–80% through seed inoculation. Eventually, potential isolate BP1.1 has showed wide pH tolerance ability ranging from pH 6–10. Further, potential isolate B. bassiana BP1.1 was evaluated for their efficacy against damping off of tomato under in vitro condition yielding higher germination percentage of 87.34% of and lower disease incidence up to 33.45% under treatment combinations. These findings provide substantial evidences on multifarious potential of B. bassiana as antagonist as well as plant growth promoter in addition to a potential entomopathogen, thus paves the way of a newer domain in the arena of crop protection.

Indian Phytopathology, 2021

Sclerotium rolfsii Sacc causes serious yield loss of Pippali (Piper longum Linn.). Catkin, the fr... more Sclerotium rolfsii Sacc causes serious yield loss of Pippali (Piper longum Linn.). Catkin, the fruit of Pippali is directly used
in medicinal industry so, use of chemical fungicides is not encourages for the cultivation of Pippali. The present study was
carried out to bring orgnaic management strategy of the crop. Six native isolates of fungal and bacterial antagonists were
tested against S. rolfsii and found T. harzianum was found as the best agents. In in vitro studies, sclerotial production was
found drastically reduced when exposed to biocontrol agents. Result on field experiment showed that soil application of
mustard oil cake (MOC) @ 1 kg/2X2 m2
plot fortified with T. harzianum @ 5 ml/m2 found effective in managing the basal
rot with disease reduction upto 10.65% with increased plant growth parameters. This was followed by soil application of T.
harzianum @ 5 ml/m2 with disease incidence of 14.98%.

Bulletin of environment, pharmacology and life sciences, 2017

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. Mechanisms of plant disease suppression by Beauveria sp. involve antibiosis, mycoparasitism, competition, endophytism and induced systemic resistance. Moreover, production of wide array of volatile organic compounds, hydrolytic enzymes (chitinases, amylases, lipases, cellulases, caseinases and proteases) and various secondary metabolites (beauvericin, bassinolide, beauvolide, beauviroloide, oosporein, bassianin and tenellin) triggers its antifungal and antimicrobial properties against various plant pathogens. Beauveria sp. have been reported to suppress diseases caused by various foliar and soil borne phytopathogens belonging to Oomycetes

Trends in biosciences, 2017

The growth of B. bassiana was tested in different solid media namely SDA (Sabouraud Dextrose Agar... more The growth of B. bassiana was tested in different solid media namely SDA (Sabouraud Dextrose Agar), PDA (Potato Dextrose Agar), Sabouraud Dextrose Yeast Agar (SDAY), Czapek Dox Agar (CDA), Oat Meal Agar (OMA), Potato Carrot Agar (PCA) and Rose Bengal Agar (RBA) on 3 rd , 5 th and 7 th day after incubation. Maximum colony diameter of 45.33 mm was observed in SDYA followed by SDB (43.00 mm). The morphological characteristics observed in SDA media were white circular colony with entire margin and smooth texture, whereas, in SDYA were white colony with undulated margin and rough texture.

Journal of pharmacognosy and phytochemistry, 2017

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. The compatibility of Beauveria isolate BP1.1 was evaluated with different fungicides namely Carbendazim (50 WP) and Copper Oxychloride (50 WP) and botanicals such as Aqueous Neem Leaf Extract (ANLE) (1% w/v), Aqueous Garlic Extract (AGE) (1% w/v) and Neem Seed Kernel Extract (NLKE) (5% w/v) at three different concentrations. The isolate of Beauveria BP1.1 showed maximum growth in Copper Oxychloride (80.50 mm) followed by Neem Leaf Extract (74.75 mm) and Neem Seed kernel Extract (70.75 mm), whereas, the least growth was observed in Garlic extract (60.50 mm) and total inhibition was observed in carbendazim.

world journal of pharmacy and pharmaceutical sciences, 2019

A new fungal species namely Paecilomyces acridomorphum on Grasshopper from the field of Central a... more A new fungal species namely Paecilomyces acridomorphum on Grasshopper from the field of Central agriculture university, Imphal, Meghalaya has been recorded to science and being described taxonomically.

The Pharma innovation , 2020

In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of fr... more In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of frogeye leaf spots, canker and twig die back that in severe cases causing dead branches and severe defoliation have been observed from apple plantation of Umiam, Meghalaya. Isolation of the associated causal fungal agent was done in selective media and its cultural, morphological as well as microscopical studies were carried out. Furthermore, confirmation of the putative fungus was done by conducting pathogenicity test to prove Koch's postulate. From the available literature, the fungus associated with the disease symptom have been identified as Diplodia seriata causing black rot of apple. To the best of the knowledge, this is the first report of isolation and pathogenicity confirmation of black rot of apple caused by D. seriata from Meghalaya, India.

Journal of pharmacognosy and phytochemistry, 2020

In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of fr... more In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of frogeye leaf spots, canker and twig die back that in severe cases causing dead branches and severe defoliation have been observed from apple plantation of Umiam, Meghalaya. Isolation of the associated causal fungal agent was done in selective media and its cultural, morphological as well as microscopical studies were carried out. Furthermore, confirmation of the putative fungus was done by conducting pathogenicity test to prove Koch's postulate. From the available literature, the fungus associated with the disease symptom have been identified as Diplodia seriata causing black rot of apple. To the best of the knowledge, this is the first report of isolation and pathogenicity confirmation of black rot of apple caused by D. seriata from Meghalaya, India.

International Journal of Current Microbiology and Applied Science, 2020

Rhizoctonia solani is one of the most important soil borne plant pathogenic fungus of many agricu... more Rhizoctonia solani is one of the most important soil borne plant pathogenic fungus of many agricultural and horticultural crops worldwide. The detailed analysis of sheath blight resistance at genetic, molecular, biochemical and
functional levels is facilitated by development of effective and uniform
inoculation techniques. The efficiency of R. solani infection plays a pivotal
role in accurate evaluation of disease and screening of resistant cultivars. In the
present study, an innovative technique for artificial inoculation of R. solani in
rice was evaluated under field conditions using maize sand meal as inoculum
type. Development of sheath blight disease symptoms was found to be
effective and uniform in inoculated plots.

Biological Forum – An International Journal, 2021

Groundnut (Arachis hypogea L., Fam: Fabaceae), an important annual leguminous and oilseed crop gr... more Groundnut (Arachis hypogea L., Fam: Fabaceae), an important annual leguminous and oilseed crop grown in an area of 7.78 m ha with an average production and productivity of 7.79 m t and 990 kg ha-1 respectively. The crop is susceptible to many fungal diseases such as tikka, rust, charcoal rot and crown rot, of which tikka disease caused by Cercospora spp. is the most destructive disease claiming 10-50 % yield losses affecting pod yield and quality. The state of Meghalaya is considered as organic state by default. But there no recommendation on organic management practices of the tikka disease of groundnut which is urgent need for the state. In the present study, a field trial was conducted to evaluate the efficacy of UmTricho, a bioformulation developed from native strain of Trichoderma harzianum of Meghalaya against tikka leaf spot disease of groundnut. Efficacy of UmTricho was compared in six different treatment combinations viz., T 1-Biopriming of seeds (10 ml UmTricho in 1000 ml water for 1 Kg seed), T 2-Soil application with UmTricho enriched compost (1 Kg of compost + 40 ml on UmTricho in 1 litre water incubate for 7 days), apply twice first at the time of seed sowing and second at canopy closure stage, T 3-Foliar spray of cow dung slurry enriched with UmTricho (100 ml in 10000 litres of water) from 15 days after germination, thrice at 15 days interval, T 4-T 1 + T 2 + T 3 , T 5-Chemical check (Bavistin @ 0.1% as foliar spray) and T 6-Uninoculated control. Combined application (T 4) with UmTricho@ 10 ml in 1 litre as seed treatment + 3 foliar sprays impregnated with enriched compost at 15 days interval starting from appearance of symptom reduced 30-40% disease incidence as compared to control. In addition, enhanced physiological parameters viz., plant biomass, pod number, pod length, root nodulation and yield were observed as compared to control (T 5).

Frontiers in Agronomy

Biological control of plant pathogens has become increasingly possible with the use of fungi, whi... more Biological control of plant pathogens has become increasingly possible with the use of fungi, which have a high reproductive rate (both sexually and asexually) and a short generation time and are very specific to their target. Trichoderma species are found in diverse habitats and experience various interactions with other organisms. They are used as bio-fungicides owing to their plant-protecting abilities, and they produce a large number of secondary metabolites (SMs) accompanied by enrichment in secondary metabolism-associated genes. This article aims to review and discuss the SMs produced by Trichoderma species, including their physiology, mode of action, mass production, and industrial and field applications for the control of plant diseases. We also discuss the evolutionary history, taxonomical gradient, classification, and ecology of Trichoderma species, as well as indirect and direct mechanisms used as plant protectors with gene improvement strategies. Aside from the bioactivi...

International Journal of Current Microbiology and Applied Sciences, 2020

The Pharma Innovation, 2020

The Pharma Innovation, 2021

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. Mechanisms of plant disease suppression by Beauveria sp. involve antibiosis, mycoparasitism, competition, endophytism and induced systemic resistance. Moreover, production of wide array of volatile organic compounds, hydrolytic enzymes (chitinases, amylases, lipases, cellulases, caseinases and proteases) and various secondary metabolites (beauvericin, bassinolide, beauvolide, beauviroloide, oosporein, bassianin and tenellin) triggers its antifungal and antimicrobial properties against various plant pathogens. Beauveria sp. have been reported to suppress diseases caused by various foliar and soil borne phytopathogens belonging to Oomycetes (Phytopthora infestans, Pythium myriotylum, P. debaryanum, P. irregular and P. ultimum ), fungi...

Research Article, 2021

Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has drawn attention worldwide a... more Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has drawn attention worldwide as potential biocontrol agent against insect-pests since decades. In the rice ecosystem, B. bassiana has been established as potential mycoinsecticide against major insect-pests of rice viz., Cnaphalocrocis medunalis, Nilaparvata lugens, Dicladispa armigera, Scirpophaga incertulas etc., but its efficacy against phytopathogens of rice in simultaneous application is least studied. Among major phytopathogens of rice, bacterial leaf blight incurs a yield loss of up to 12-75%, affecting crop at seedling, tillering and vegetative stages. Therefore, recent studies have inclined towards unravelling antimicrobial potential of B. bassiana as effective plant disease antagonist, thereby, opening a newer dimension in dualpurpose crop protection strategies. In the present study, antagonistic abilities of fifteen (15) native isolates of B. bassiana were evaluated against Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice by conducting agar-plug diffusion and inverted plate assays. The results showed that, majority of B. bassiana isolates viz., Bb31, Bb45, Bb48, Bb25, Bb53 were able to inhibit bacterial growth, with maximum per cent inhibition was recorded in the range of 60-84% through development of inhibition zone. Further, antibacterial ability of B. bassiana was also attributed to release of non-volatile inhibitory metabolites responsible for the formation of inhibition zone on the bacterial lawn. These findings provide substantial evidences on antibacterial abilities of B. bassiana against X. oryzae pv. oryzae and further provides a scope to test the efficacy of B. bassiana against bacterial leaf blight of rice in the glasshouse and field condition in addition to their potential entomogenous behaviour against insect-pests of rice.

Phytopathology, 2020

Peach, (Prunus persica (L) Batsch.) is one of the most important stone fruit commercially grown i... more Peach, (Prunus persica (L) Batsch.) is one of the most important stone fruit commercially grown in the hills including Meghalaya. During Feb-June, 2020 in a roving survey we have found infection on twigs and leaves of peach at Umiam and Pepbah of Ribhoi and East Khasi Hills district of Meghalaya. The infected part showing symptoms of twig blight, blossom blight, cankers and fruit rot. Samples of young and matured leaf, blossom, twigs etc with infection were collected. We studied the detailed symptomatology, isolated and purified the associated causal organism. Through Koch’s postulate we confirmed the pathogenic association of the causal agent. Further through cultural, morphological, and micrometry study the microorganism was identified as Monilinia fructicola (G. Winter) Honey and this is the first report from the Meghalaya of North East India.

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. The compatibility of Beauveria isolate BP1.1 was evaluated with different fungicides namely Carbendazim (50 WP) and Copper Oxychloride (50 WP) and botanicals such as Aqueous Neem Leaf Extract (ANLE) (1% w/v), Aqueous Garlic Extract (AGE) (1% w/v) and Neem Seed Kernel Extract (NLKE) (5% w/v) at three different concentrations. The isolate of Beauveria BP1.1 showed maximum growth in Copper Oxychloride (80.50 mm) followed by Neem Leaf Extract (74.75 mm) and Neem Seed kernel Extract (70.75 mm), whereas, the least growth was observed in Garlic extract (60.50 mm) and total inhibition was observed in carbendazim.

Indian Phytopathology, 2021

Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has widely drawn attention of c... more Beauveria bassiana (Balsamo) Vuillemin, a white muscardine fungus has widely drawn attention of crop protection practitioners as potential biocontrol agent against insect-pests since decades. In the present study, antagonistic potential of 22 native of B. bassiana isolates were evaluated against damping-off disease of tomato caused by Pythium sp. Results showed that all B. bassiana isolates were able to inhibit mycelial growth of P. myriotylum to the extent of 68–82%. Further studies were conducted to understand the ability of screened B. bassiana isolates to produce cell wall degrading enzymes (CWDEs) viz., amylase, caesinase, chitinase, cellulase, lipase and protease respectively. The potential B. bassiana isolates showed dynamic colonization efficiency in tomato plants with colonization percentage (%) as high as 72–80% through seed inoculation. Eventually, potential isolate BP1.1 has showed wide pH tolerance ability ranging from pH 6–10. Further, potential isolate B. bassiana BP1.1 was evaluated for their efficacy against damping off of tomato under in vitro condition yielding higher germination percentage of 87.34% of and lower disease incidence up to 33.45% under treatment combinations. These findings provide substantial evidences on multifarious potential of B. bassiana as antagonist as well as plant growth promoter in addition to a potential entomopathogen, thus paves the way of a newer domain in the arena of crop protection.

Indian Phytopathology, 2021

Sclerotium rolfsii Sacc causes serious yield loss of Pippali (Piper longum Linn.). Catkin, the fr... more Sclerotium rolfsii Sacc causes serious yield loss of Pippali (Piper longum Linn.). Catkin, the fruit of Pippali is directly used
in medicinal industry so, use of chemical fungicides is not encourages for the cultivation of Pippali. The present study was
carried out to bring orgnaic management strategy of the crop. Six native isolates of fungal and bacterial antagonists were
tested against S. rolfsii and found T. harzianum was found as the best agents. In in vitro studies, sclerotial production was
found drastically reduced when exposed to biocontrol agents. Result on field experiment showed that soil application of
mustard oil cake (MOC) @ 1 kg/2X2 m2
plot fortified with T. harzianum @ 5 ml/m2 found effective in managing the basal
rot with disease reduction upto 10.65% with increased plant growth parameters. This was followed by soil application of T.
harzianum @ 5 ml/m2 with disease incidence of 14.98%.

Bulletin of environment, pharmacology and life sciences, 2017

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. Mechanisms of plant disease suppression by Beauveria sp. involve antibiosis, mycoparasitism, competition, endophytism and induced systemic resistance. Moreover, production of wide array of volatile organic compounds, hydrolytic enzymes (chitinases, amylases, lipases, cellulases, caseinases and proteases) and various secondary metabolites (beauvericin, bassinolide, beauvolide, beauviroloide, oosporein, bassianin and tenellin) triggers its antifungal and antimicrobial properties against various plant pathogens. Beauveria sp. have been reported to suppress diseases caused by various foliar and soil borne phytopathogens belonging to Oomycetes

Trends in biosciences, 2017

The growth of B. bassiana was tested in different solid media namely SDA (Sabouraud Dextrose Agar... more The growth of B. bassiana was tested in different solid media namely SDA (Sabouraud Dextrose Agar), PDA (Potato Dextrose Agar), Sabouraud Dextrose Yeast Agar (SDAY), Czapek Dox Agar (CDA), Oat Meal Agar (OMA), Potato Carrot Agar (PCA) and Rose Bengal Agar (RBA) on 3 rd , 5 th and 7 th day after incubation. Maximum colony diameter of 45.33 mm was observed in SDYA followed by SDB (43.00 mm). The morphological characteristics observed in SDA media were white circular colony with entire margin and smooth texture, whereas, in SDYA were white colony with undulated margin and rough texture.

Journal of pharmacognosy and phytochemistry, 2017

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. The compatibility of Beauveria isolate BP1.1 was evaluated with different fungicides namely Carbendazim (50 WP) and Copper Oxychloride (50 WP) and botanicals such as Aqueous Neem Leaf Extract (ANLE) (1% w/v), Aqueous Garlic Extract (AGE) (1% w/v) and Neem Seed Kernel Extract (NLKE) (5% w/v) at three different concentrations. The isolate of Beauveria BP1.1 showed maximum growth in Copper Oxychloride (80.50 mm) followed by Neem Leaf Extract (74.75 mm) and Neem Seed kernel Extract (70.75 mm), whereas, the least growth was observed in Garlic extract (60.50 mm) and total inhibition was observed in carbendazim.

world journal of pharmacy and pharmaceutical sciences, 2019

A new fungal species namely Paecilomyces acridomorphum on Grasshopper from the field of Central a... more A new fungal species namely Paecilomyces acridomorphum on Grasshopper from the field of Central agriculture university, Imphal, Meghalaya has been recorded to science and being described taxonomically.

The Pharma innovation , 2020

In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of fr... more In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of frogeye leaf spots, canker and twig die back that in severe cases causing dead branches and severe defoliation have been observed from apple plantation of Umiam, Meghalaya. Isolation of the associated causal fungal agent was done in selective media and its cultural, morphological as well as microscopical studies were carried out. Furthermore, confirmation of the putative fungus was done by conducting pathogenicity test to prove Koch's postulate. From the available literature, the fungus associated with the disease symptom have been identified as Diplodia seriata causing black rot of apple. To the best of the knowledge, this is the first report of isolation and pathogenicity confirmation of black rot of apple caused by D. seriata from Meghalaya, India.

Journal of pharmacognosy and phytochemistry, 2020

In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of fr... more In a survey conducted during Januray-May, 2020, apple trees showing characteristic symptoms of frogeye leaf spots, canker and twig die back that in severe cases causing dead branches and severe defoliation have been observed from apple plantation of Umiam, Meghalaya. Isolation of the associated causal fungal agent was done in selective media and its cultural, morphological as well as microscopical studies were carried out. Furthermore, confirmation of the putative fungus was done by conducting pathogenicity test to prove Koch's postulate. From the available literature, the fungus associated with the disease symptom have been identified as Diplodia seriata causing black rot of apple. To the best of the knowledge, this is the first report of isolation and pathogenicity confirmation of black rot of apple caused by D. seriata from Meghalaya, India.

International Journal of Current Microbiology and Applied Science, 2020

Rhizoctonia solani is one of the most important soil borne plant pathogenic fungus of many agricu... more Rhizoctonia solani is one of the most important soil borne plant pathogenic fungus of many agricultural and horticultural crops worldwide. The detailed analysis of sheath blight resistance at genetic, molecular, biochemical and
functional levels is facilitated by development of effective and uniform
inoculation techniques. The efficiency of R. solani infection plays a pivotal
role in accurate evaluation of disease and screening of resistant cultivars. In the
present study, an innovative technique for artificial inoculation of R. solani in
rice was evaluated under field conditions using maize sand meal as inoculum
type. Development of sheath blight disease symptoms was found to be
effective and uniform in inoculated plots.

Biological Forum – An International Journal, 2021

Groundnut (Arachis hypogea L., Fam: Fabaceae), an important annual leguminous and oilseed crop gr... more Groundnut (Arachis hypogea L., Fam: Fabaceae), an important annual leguminous and oilseed crop grown in an area of 7.78 m ha with an average production and productivity of 7.79 m t and 990 kg ha-1 respectively. The crop is susceptible to many fungal diseases such as tikka, rust, charcoal rot and crown rot, of which tikka disease caused by Cercospora spp. is the most destructive disease claiming 10-50 % yield losses affecting pod yield and quality. The state of Meghalaya is considered as organic state by default. But there no recommendation on organic management practices of the tikka disease of groundnut which is urgent need for the state. In the present study, a field trial was conducted to evaluate the efficacy of UmTricho, a bioformulation developed from native strain of Trichoderma harzianum of Meghalaya against tikka leaf spot disease of groundnut. Efficacy of UmTricho was compared in six different treatment combinations viz., T 1-Biopriming of seeds (10 ml UmTricho in 1000 ml water for 1 Kg seed), T 2-Soil application with UmTricho enriched compost (1 Kg of compost + 40 ml on UmTricho in 1 litre water incubate for 7 days), apply twice first at the time of seed sowing and second at canopy closure stage, T 3-Foliar spray of cow dung slurry enriched with UmTricho (100 ml in 10000 litres of water) from 15 days after germination, thrice at 15 days interval, T 4-T 1 + T 2 + T 3 , T 5-Chemical check (Bavistin @ 0.1% as foliar spray) and T 6-Uninoculated control. Combined application (T 4) with UmTricho@ 10 ml in 1 litre as seed treatment + 3 foliar sprays impregnated with enriched compost at 15 days interval starting from appearance of symptom reduced 30-40% disease incidence as compared to control. In addition, enhanced physiological parameters viz., plant biomass, pod number, pod length, root nodulation and yield were observed as compared to control (T 5).

Today and tomorrow printers and publishers, 2019

Entomopathogenic fungi (EPF) are considered as potential biocontrol agents against various insect... more Entomopathogenic fungi (EPF) are considered as potential biocontrol agents against various insect pests since late nineteenth century. Since then more than 170 mycoinsecticides have been produced with at least 12 species, out of over 800 fungal species identified as pathogenic to insects. The genera which have been exploited commercially are Beauveria bassiana, B. Brongniartii, Metarhizium anisopliae, M. flavoviridae, Lecanicillium lecanii, Isaria fumosorosea, Nomuraea rileyi, Hirsutella thompsonii etc. Farmers usually rely on use of chemicals against pests and diseases. However, injudicious application of chemicals over many years for crop protection is the serious concern for environment and health hazards. Moreover, such practices also deteriorate the soil health, disturb ecological balance and led to the development of resistance. To overcome these problems, there is a need to develop alternative control methods to suppress pests and plant pathogens. Recent studies established that EPF play additional roles such as antagonists against plant pathogens remain as endophyte, act as plant growth promoter and efficient rhizosphere colonizer. Such additional roles played by EPF provide opportunities for the dual purpose use as biocontrol agent (BCA) in integrated pest management (IPM) as well as in integrated disease management (IDM) strategies. This current chapter reviews various potential EPF genera, their characteristics and effective application against plant pathogens, mechanisms of antagonism, distribution in nature, endophytic directions for the use of EPF as dual microbial control agents, alternative to synthetic chemicals are summarized.

Vigyan Varta, 2020

Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention w... more Beauveria bassiana is the most widely studied entomopathogenic fungi which have drawn attention worldwide not only as an insect-pest control but also as management of plant pathogenic fungi and bacteria along with their plant growth promotion activities. In addition to its entomopathogenic properties, this fungus is also being exploited for its other beneficial properties like plant endophytes, antagonists for plant diseases, beneficial rhizosphere colonizers and plant growth promoters.