Avni Blotra | PUNJAB TECHNICAL UNIVERSITY, JALANDHAR, PUNJAB, INDIA (original) (raw)
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Hazara University Mansehra, Dhodial KPK Pakistan
Università Cattolica del Sacro Cuore (Catholic University of the Sacred Heart)
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Papers by Avni Blotra
Journal of Proteome Research, Dec 14, 2022
ABSTRACTEchis carinatusis a widely distributed venomous snake in the Indian subcontinent. It is o... more ABSTRACTEchis carinatusis a widely distributed venomous snake in the Indian subcontinent. It is one of the “Big Four” snake species responsible for mortality and severe health complications caused by envenomations. Given the significance of the species for human health, we set out to assemble the whole genome ofE. carinatus. Using only long reads for assembly and short reads for correction, we achieved a highly contiguous 1.57Gb assembly, with a contig N50 of 17.1 Mb and scaffold N50 of 23.4 Mb. We could map 96.58% of our reads back to the assembled genome, and Benchmarking Universal Single-Copy Orthologs (BUSCO) score of 97.4%, indicate near completeness. 48.59% of the genome was repeat-masked using a combination of de novo and annotated repeat databases. The predominant types of repeats include retrotransposons (LINEs, SINEs and LTR elements), DNA transposons and satellite DNA (SSRs). Our work would serve as a resource to understand the role of genes responsible for venom related ...
Journal of Proteome Research
AL-Kindy College Medical Journal, 2019
Background: Ultrasound provides a powerful tool for assessing early pregnancy and detecting preg... more Background: Ultrasound provides a powerful tool for assessing early pregnancy and detecting pregnancy failure at first trimester and promoting rapid effective management. Several criteria have been established to predict the pregnancy outcome particularly in symptomatic women . Aim: To evaluate asymptomatic women at the first trimester of pregnancy , to assess the efficacy of certain ratios as mid sac diameter (MSD) / yolk sac ratio & crown rump length (CRL)/mid sac diameter (as indicator of early first trimester oligohydramnios) in predicting poor 1st trimester outcome Type of the study: A cross-sectional study. Patients & Methods: A sixty three asymptomatic women were enrolled in this prospective study . Transvaginal sonography was performed for confirmation of pregnancy viability & exclusion of multiple pregnancies 6-8 weeks of gestation & follow up ultrasound repeated at the beginning of second trimester to confirm the continuation of pregnancy and viability of fetus...
Toxins
In India, polyvalent antivenom is the mainstay treatment for snakebite envenoming. Due to batch-t... more In India, polyvalent antivenom is the mainstay treatment for snakebite envenoming. Due to batch-to-batch variation in antivenom production, manufacturers have to estimate its efficacy at each stage of IgG purification using the median effective dose which involves 100–120 mice for each batch. There is an urgent need to replace the excessive use of animals in snake antivenom production using in vitro alternatives. We tested the efficacy of a single batch of polyvalent antivenom from VINS bioproducts limited on Echis carinatus venom collected from three different locations—Tamil Nadu (ECVTN), Goa (ECVGO) and Rajasthan (ECVRAJ)—using different in vitro assays. Firstly, size-exclusion chromatography (SEC-HPLC) was used to quantify antivenom–venom complexes to assess the binding efficiency of the antivenom. Secondly, clotting, proteolytic and PLA2 activity assays were performed to quantify the ability of the antivenom to neutralize venom effects. The use of both binding and functional as...
Journal of Proteome Research, Dec 14, 2022
ABSTRACTEchis carinatusis a widely distributed venomous snake in the Indian subcontinent. It is o... more ABSTRACTEchis carinatusis a widely distributed venomous snake in the Indian subcontinent. It is one of the “Big Four” snake species responsible for mortality and severe health complications caused by envenomations. Given the significance of the species for human health, we set out to assemble the whole genome ofE. carinatus. Using only long reads for assembly and short reads for correction, we achieved a highly contiguous 1.57Gb assembly, with a contig N50 of 17.1 Mb and scaffold N50 of 23.4 Mb. We could map 96.58% of our reads back to the assembled genome, and Benchmarking Universal Single-Copy Orthologs (BUSCO) score of 97.4%, indicate near completeness. 48.59% of the genome was repeat-masked using a combination of de novo and annotated repeat databases. The predominant types of repeats include retrotransposons (LINEs, SINEs and LTR elements), DNA transposons and satellite DNA (SSRs). Our work would serve as a resource to understand the role of genes responsible for venom related ...
Journal of Proteome Research
AL-Kindy College Medical Journal, 2019
Background: Ultrasound provides a powerful tool for assessing early pregnancy and detecting preg... more Background: Ultrasound provides a powerful tool for assessing early pregnancy and detecting pregnancy failure at first trimester and promoting rapid effective management. Several criteria have been established to predict the pregnancy outcome particularly in symptomatic women . Aim: To evaluate asymptomatic women at the first trimester of pregnancy , to assess the efficacy of certain ratios as mid sac diameter (MSD) / yolk sac ratio & crown rump length (CRL)/mid sac diameter (as indicator of early first trimester oligohydramnios) in predicting poor 1st trimester outcome Type of the study: A cross-sectional study. Patients & Methods: A sixty three asymptomatic women were enrolled in this prospective study . Transvaginal sonography was performed for confirmation of pregnancy viability & exclusion of multiple pregnancies 6-8 weeks of gestation & follow up ultrasound repeated at the beginning of second trimester to confirm the continuation of pregnancy and viability of fetus...
Toxins
In India, polyvalent antivenom is the mainstay treatment for snakebite envenoming. Due to batch-t... more In India, polyvalent antivenom is the mainstay treatment for snakebite envenoming. Due to batch-to-batch variation in antivenom production, manufacturers have to estimate its efficacy at each stage of IgG purification using the median effective dose which involves 100–120 mice for each batch. There is an urgent need to replace the excessive use of animals in snake antivenom production using in vitro alternatives. We tested the efficacy of a single batch of polyvalent antivenom from VINS bioproducts limited on Echis carinatus venom collected from three different locations—Tamil Nadu (ECVTN), Goa (ECVGO) and Rajasthan (ECVRAJ)—using different in vitro assays. Firstly, size-exclusion chromatography (SEC-HPLC) was used to quantify antivenom–venom complexes to assess the binding efficiency of the antivenom. Secondly, clotting, proteolytic and PLA2 activity assays were performed to quantify the ability of the antivenom to neutralize venom effects. The use of both binding and functional as...