Oleksii Khudyi | Yuriy Fedkovych Chernivtsi National University (original) (raw)

Papers by Oleksii Khudyi

Health, sport, rehabilitation

Purpose To study the peculiarities of adaptive and compensatory reactions in mature women after p... more Purpose To study the peculiarities of adaptive and compensatory reactions in mature women after prolonged hypokinesia using various kinesiological models of the neuromuscular system readaptation. Material and methods 52 women of mature age (39-46 years) after long-term (6-7 years) hypokinesia took part in the study. Group A used a kinesiological model based on free weight exercises with changes in kinematic and dynamic characteristics. Group B used a model based on machine exercises. The following methods were used to monitor adaptation processes: bioelectrical impedance analysis, anthropometry, testing of strength capabilities, biochemical blood analysis (cortisol and creatinine concentration). Results The dynamics of the studied indicators differed significantly between the groups using the proposed kinesiological models for 12 weeks. Thus, the fat-free and body cell mass indicators were 5 times higher in group A women compared to group B participants. The body fat mass decreased ...

Fifteenth International Conference on Correlation Optics

Under optimal conditions, the division of unicellular cyanobacteria lasts 6-12 hours, so during t... more Under optimal conditions, the division of unicellular cyanobacteria lasts 6-12 hours, so during the growing season there is an avalanche of reproduction. It also leads to increased exports of their metabolites to the environment. The toxins of Cyanobacterial after the destruction of these cells enter the aquatic environment and cause poisoning of aquatic organisms and animals and people who consume poisoned water. To avoid the release of toxins, it is necessary to control the rate of accumulation of cyanobacterial biomass. It is proposed to use titanium oxide (IV) as an adsorbent to regulate the number of cyanobacteria. TiO2 has a number of advantages: chemical and biological inertness, non-toxicity, high photostability. The aim of the work was to involve methods of microscopy of native and stained drugs to assess the physiological state of Microcystis pulverea (H.C. Wood) Forti under the action of TiO2. Microcystis pulverea was grown on Fitzgerald medium in the modification of Zender and Gorham №11, at a temperature of 21 ° C and a 16-hour photoperiod in a climatic room. To the cell biomass (4.5 x 106 cells / ml) was made white powder TIO2 in different concentrations: 0.5%, 1%, 2.5%, 5%. To assess the condition of M. pulverea cells after exposure to titanium (IV) oxide, microscopy of objects was performed. Native cells were analyzed for the presence of protective mu-cous formations. The localization of mucous formations on the surface of TiO2 was evaluated. Differential staining was used to determine the presence of living and dead cells and the number of metabolically active cells. These indicators make it possible to assess the condition of cyanobacterial cells under the influence of various environmental factors. To deter-mine the number of dead cells, the cells were stained with vital dyes (methylene blue and neutral red - 1: 5000). To determine the number of physiologically active cells and cytochrome oxidase test were performed according to standard methods. In both cases, cells were counted using a Fuchs-Rosenthal camera and a Micromed XS-3300 trinocular microscope. In samples with high concentrations of titanium dioxide, the cells remained unprotected. It is noted that the mucus was adsorbed around TIO2. The lowest degree of adsorption was characterized by samples containing 0.5% TiO2, and the highest degree - with 5% TiO2. When using TiO2, more than 2.5% can completely release cells of cyanobacteria in the aqueous medium from the mucous that protects their cells, and then use other drugs for lysis. An increase in the number of dead cells was observed in cyanobacterial culture. In M. pulverea culture, the number of metabolically active cells decreases sharply with increasing concentration of TiO2. Incubation with TiO2 led to a gradual decrease in the number of metabolically active cells: from 96% (in the control sample) to about 9% in the presence of 5% TiO2. Although this culture remains relatively alive, but loses the ability to actively metabolize. It can be predicted that over time the number of M. pulverea cells will decrease sharply, which will lead to the death of the culture as a whole.

Fifteenth International Conference on Correlation Optics

With the help of optical methods of fish functional state diagnostics the possibility of using DO... more With the help of optical methods of fish functional state diagnostics the possibility of using DON-1R, a drug that contains γ–crotonolactone, in recirculating aquaculture system was proven. It was stated that adding DON-1R to granulated feed while feeding sturgeon fish stimulates the raise in mass accumulating velocity. Moreover, microscopical analysis of histological cuts and detailed analysis of their photomicrographs with the use of various approaches in contrasting images showed that feeding with feed with DON-1R is accompanied with reactive changes to the liver. In particular, fish that received the tested drug had the inequable liver cytoarchitectonics, chaotic hepatocytes placement, radial character of liver channels and undefined sinusoids. Hepatocytes of experimental fish had large hyperchromatic nuclei with 4,5 μm diameter with multiple tiny defined nucleoli. Control fish’s nuclei were 1,5 times smaller, had even coloring and 1-2 nucleoli. Moreover, fish from experimental group had signs of partial acidophilic coagulation, such as occurrence of homogenous pink lumps in cytoplasm (Mallory bodies). It is worth noting that the application of DON-1R does not lead to deep destructive changes in liver structure, such as necrosis of fibrosis. Taking all of the above into consideration, DON-1R is the most effective for growth stimulation if applied for a short period of time for commercial growing. Optimal period of use is 20 days.

The dataset consists of records of protected plant and fungi species published in a series of pub... more The dataset consists of records of protected plant and fungi species published in a series of publications specially prepared by the Institute of Zoology: 1) Materials for the 4th edition of the Red Book of Ukraine. Fauna / Series: «Conservation Biology in Ukraine». – Vol. 6, Т. 1. – Kyiv, I. I. Schmalhausen institute of zoology NAS of Ukraine, 2018. – 438 p. 2) Materials for the 4th edition of the Red Book of Ukraine. Fauna / Series: «Conservation Biology in Ukraine». – Vol. 6, Т. 2. – Kyiv, I. I. Schmalhausen institute of zoology NAS of Ukraine, 2018. – 450 p. 3) Materials for the 4th edition of the Red Book of Ukraine. Fauna (Series: «Conservation Biology in Ukraine». – Vol. 7, T. 3). - Kyiv, 2019. - 416 p.

The article presents the results of a study of the regularities of the scattering of polarized li... more The article presents the results of a study of the regularities of the scattering of polarized light by fish mucus and fish scales in order to identify matrix elements that are most sensitive to changes in the conformation, shape and size of protein macromolecules of fish mucus, and to identify the structural features of fish scales.

Biolohichni systemy, Dec 23, 2020

<jats:p>We studied the possibility of using low frequency laser emission to improve product... more <jats:p>We studied the possibility of using low frequency laser emission to improve production properties of feed hydrobionts, in particular microalgae Desmodesmus armatus and Daphnia magna, which are highly applied in aquaculture. It was shown that low-frequency laser emission with the wavelength 650 nm has a positive influence on production properties of both phyto- and zooplankton. In 24 hours the biomass of irradiated D. armatus was 25% larger than non-irradiated one. Meanwhile, application λ 420 and 530 nm did not show credible accumulation of microalgae biomass. If it comes to daphnia, positive dynamic in population density enlarging was observed only since 5-7th day of cultivation, depending on emission exposition. Differences in low-frequency laser emission with λ 420 and 530 efficacy on D. magna production properties were observed while using different exposition. Thus, maximal culture density was observed when λ 420 nm for 90 s and λ 650 nm for 60 s were applied. Longer irradiation with red laser did not have positive effect. It was established that in daphnia tissues, which were influenced by λ 420 nm laser emission, TBA-active products were accumulating more. Meanwhile, for daphnias, which were irradiated with red laser, TBA amount was not different from the control group one. Catalase activity increased while applying λ 650 nm laser emission. On the other hand, λ 420 nm did not cause credible increasing of catalase activity in comparison with control group. Taking received results about photobiological effects of low-frequency laser emission on D. armatus and D. magna into consideration, both biomasses enlargement during co-cultivation can be obtained if wavelength 650 nm is used.</jats:p>

Archives of Polish Fisheries, 2018

The first aim of the study was to determine the impact the commercial product S.presso (INVE Aqua... more The first aim of the study was to determine the impact the commercial product S.presso (INVE Aquaculture, Belgium) had on enriching the proximate composition of brine shrimp (Artemia sp.). The second aim of the study was to develop a better enrichment protocol for preparing enriched brine shrimp larvae. Two different enrichment protocols were investigated; the supplement was administered in either one (0.5 g L-1) or two equal (0.25 g L-1) doses. Using S.presso to enrich brine shrimp nauplii with polyunsaturated fatty acids resulted in higher levels of total lipids and higher quantities of docosahexaenoic acid DHA and eicosapentaenoic acid EPA deposition. Additionally, the enrichment supplement did not lead to overall lowered protein content values caused by increased hydrolytic activity that likely facilitates digestion in larval fish as they shift to exogenous feeding.

International Letters of Natural Sciences, 2016

The results of using the waste water from recirculating aquaculture system for phyto- and zooplan... more The results of using the waste water from recirculating aquaculture system for phyto- and zooplankton cultivation are presented in the article. The physico-chemical parameters of the aquaculture system’s waste water and comparative culture media were recorded during hydrobionts cultivation. The indicators of productivity and the biochemical composition of investigated cultures were determined. The biochemical analysis of studied cultures included the investigations of the content of total proteins, lipids, carbohydrates, carotenoids and photosynthetic pigments. Using aquaculture system’s waste water as a medium for the cultivation of phyto- and zooplankton can significantly reduce the cost of technology for live feed biomass increase in the aquaculture industries.

Fisheries & Aquatic Life, 2020

This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae bioma... more This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae biomass and then using this method to correct the nutritional composition of Artemia. Accordingly, the influence of three species of microalgal monocultures was analyzed (2 freshwater – Desmodesmus armatus (Chod.) Hegew. and Chlorella vulgaris Veijerinck; one halophilous – Dunaliella viridis Teodor.). The algal monocultures were applied once in a quantity of 2-3 × 106 cells × l−1 for each 200,000 Artemia individuals hatched. The control group Artemia did not receive algae. The enrichment process lasted 24 h, and control measurements were performed every 6 h. The survival of Artemia nauplii, their proteolytic activity, and the content of proteins, lipids, and carotenoids were analyzed. The choice of algae species for Artemia enrichment was guided by the size of the algal cells and their biochemical composition. Selected algae contained about 50% protein, 20% lipid, and 12 mg of carotenoids per...

Fisheries & Aquatic Life, 2020

Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of esse... more Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of essential compounds such as carotenoids. The possibility of increasing the productivity of carotenogenic yeast, Rhodotorula glutinis, for further bioencapsulation of zooplankton Daphnia magna is shown. An association of a UV irradiated yeast strain with lactic acid bacteria was cultivated for this purpose. This permitted intensifying the carotenogenesis of yeast, and the content of â-carotene increased by 1.7 times, and that of torularodine by 2.3 times compared with the native monoculture. The use of the association of microorganisms as a feed substrate in the cultivation of Daphnia magna provided both an increase in the carotenoid content in the investigated crustaceans more than 8 times, and their biomass by 2.5 times compared with the standard feed of Saccharomyces cerevisiae.

Biolohichni systemy, 2020

The anthibiotics use in aquaculture these days is severely restricted by European standarts of ma... more The anthibiotics use in aquaculture these days is severely restricted by European standarts of marketable fish products quality. According to this, one of the most efficient alternatives are probiotics. So the search of applicable probiotic microorganisms that can be applied in aquaculture is relevant. On the other side, probiotics introduction into the fish body is usually accomplished in composition with dry feed as a biofilm. But a lot of fish larvae are not capable of consuming dry artificial feed due to numerous reasons. For their transmission for endogenic nourishment from exogenic live feeds are used in aquaculture, which have a higher level of digestibility compared to granulated feed. One more advantage of live feed is that they can be used as vectors to provide the targeted delivery of probiotics into fish body. In this work Lactobacillus casei IMV 7280 was firsly tested for the needs of aquaculture. It is shown that the usage of this probiotic during the growing of carp w...

Aquaculture Research, 2018

Successful breeding of fish species in aquaculture depends on several factors, among which the te... more Successful breeding of fish species in aquaculture depends on several factors, among which the temperature and feed are the most significant ones. This study was designed to evaluate the effect of temperature in the range of 14–18°C on the rate of embryogenesis and duration of larva period and to estimate the efficiency of Artemia nauplii enriched with PUFAs in growing sturgeon larvae. The temperature of 16°C is the most suitable for both egg incubation and Atlantic sturgeon prelarvae maintenance under aquaculture conditions. Even minor temperature fluctuations of 1 degree up or down leads to increased loss both of eggs and prelarvae. Increased temperature shortens the incubation period but has a lesser impact on the duration of prelarvae onset of external feeding. The technology of Artemia nauplii bioencapsulation with a PUFA‐containing supplement in A. oxyrinchus rearing increases sturgeon larvae weight by 1.5 times at a constant survival dynamic.

The research work is meant to investigate the possibility of using the zeolite-containing tuffs i... more The research work is meant to investigate the possibility of using the zeolite-containing tuffs in the technology of live fish feed cultivation by example of Cladocera. It is shown that the use of natural and treated at the temperature of 150°C basaltic tuffs from „Polytske-2” basalt deposit during Moina macrocopa cultivation leads to an increase of the culture density by 2 and 2.3 times, respectively. In the experiment with Simocephalus vetulus the increase of culture density has not been found, however basaltic tuffs allow to stabilize the culture growth and slow its extinction rate. Besides, it is observed the effective purification of cultural medium of dissolved nitrogen forms probably by adsorption. The use of basaltic tuffs, calcined at a temperature of 1000 °C, leads to the death of M. macrocopa and S. vetulus cultures in 6 days. This circumstance makes the use of calcined tuffs impractical.

Fisheries & Aquatic Life, 2020

This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae bioma... more This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae biomass and then using this method to correct the nutritional composition of Artemia. Accordingly, the influence of three species of microalgal monocultures was analyzed (2 freshwater – Desmodesmus armatus (Chod.) Hegew. and Chlorella vulgaris Veijerinck; one halophilous – Dunaliella viridis Teodor.). The algal monocultures were applied once in a quantity of 2-3 × 106 cells × l−1 for each 200,000 Artemia individuals hatched. The control group Artemia did not receive algae. The enrichment process lasted 24 h, and control measurements were performed every 6 h. The survival of Artemia nauplii, their proteolytic activity, and the content of proteins, lipids, and carotenoids were analyzed. The choice of algae species for Artemia enrichment was guided by the size of the algal cells and their biochemical composition. Selected algae contained about 50% protein, 20% lipid, and 12 mg of carotenoids per...

The maintenance of live feed in automatic feeders is accompanied by fasting of fodder organisms, ... more The maintenance of live feed in automatic feeders is accompanied by fasting of fodder organisms, resultedin a lose of their nutritional value and even death. The way out of this situation can be the joint maintenance of Artemia nauplii and their fodder objects – microalgae. Accordingly, an influence of three microalgae monocultures (Desmodesmus armatus, Chlorella vulgaris, Dunaliella salina) and the AlgaMac Protein Plus drug on survival of Artemia nauplii was analyzed. It was shown that the lowest mortality rates of brine shrimp were observed when it was co-cultivated with monoculture of Chlorella. The use of the AlgaMac Protein Plus algal drug provided a significant reduction in the mortality rate of Artemia nauplii at 12 hours of cultivation. Cell size of studied algal species is a key point for their using as a fodder object for brine shrimp. Maximum diameter of Chlorella cells reaches 10 μm. Cells of D. armatus are single nucleated autospores with coccoid shape. Their size is 10...

International Letters of Natural Sciences

The nutritional composition and amino acid profile ofMoina macrocopawere studied using different ... more The nutritional composition and amino acid profile ofMoina macrocopawere studied using different types of yeast (Saccharomyces cerevisiaeandRhodotorula glutinis) as fodder substrates. The effective accumulation of carotenoids inMoina macrocopaduringR. glutinisyeast application was not accompanied by deterioration in the nutritional value of zooplankton. The content of total proteins and total lipids inMoinagrown onS. cerevisiaeandR. glutiniswas not significantly different. However, the use ofR. glutinisin the cultivation ofM. macrocopaled to the change in the ratio of proteinogenic amino acids in the studied cladocerans. In particular, the share of methionine, leucine and isoleucine significantly increased. It allowed to enhance the quality of protein in the fodder zooplankton, that is especially important in the feeding of fish fry.

Health, sport, rehabilitation

Purpose To study the peculiarities of adaptive and compensatory reactions in mature women after p... more Purpose To study the peculiarities of adaptive and compensatory reactions in mature women after prolonged hypokinesia using various kinesiological models of the neuromuscular system readaptation. Material and methods 52 women of mature age (39-46 years) after long-term (6-7 years) hypokinesia took part in the study. Group A used a kinesiological model based on free weight exercises with changes in kinematic and dynamic characteristics. Group B used a model based on machine exercises. The following methods were used to monitor adaptation processes: bioelectrical impedance analysis, anthropometry, testing of strength capabilities, biochemical blood analysis (cortisol and creatinine concentration). Results The dynamics of the studied indicators differed significantly between the groups using the proposed kinesiological models for 12 weeks. Thus, the fat-free and body cell mass indicators were 5 times higher in group A women compared to group B participants. The body fat mass decreased ...

Fifteenth International Conference on Correlation Optics

Under optimal conditions, the division of unicellular cyanobacteria lasts 6-12 hours, so during t... more Under optimal conditions, the division of unicellular cyanobacteria lasts 6-12 hours, so during the growing season there is an avalanche of reproduction. It also leads to increased exports of their metabolites to the environment. The toxins of Cyanobacterial after the destruction of these cells enter the aquatic environment and cause poisoning of aquatic organisms and animals and people who consume poisoned water. To avoid the release of toxins, it is necessary to control the rate of accumulation of cyanobacterial biomass. It is proposed to use titanium oxide (IV) as an adsorbent to regulate the number of cyanobacteria. TiO2 has a number of advantages: chemical and biological inertness, non-toxicity, high photostability. The aim of the work was to involve methods of microscopy of native and stained drugs to assess the physiological state of Microcystis pulverea (H.C. Wood) Forti under the action of TiO2. Microcystis pulverea was grown on Fitzgerald medium in the modification of Zender and Gorham №11, at a temperature of 21 ° C and a 16-hour photoperiod in a climatic room. To the cell biomass (4.5 x 106 cells / ml) was made white powder TIO2 in different concentrations: 0.5%, 1%, 2.5%, 5%. To assess the condition of M. pulverea cells after exposure to titanium (IV) oxide, microscopy of objects was performed. Native cells were analyzed for the presence of protective mu-cous formations. The localization of mucous formations on the surface of TiO2 was evaluated. Differential staining was used to determine the presence of living and dead cells and the number of metabolically active cells. These indicators make it possible to assess the condition of cyanobacterial cells under the influence of various environmental factors. To deter-mine the number of dead cells, the cells were stained with vital dyes (methylene blue and neutral red - 1: 5000). To determine the number of physiologically active cells and cytochrome oxidase test were performed according to standard methods. In both cases, cells were counted using a Fuchs-Rosenthal camera and a Micromed XS-3300 trinocular microscope. In samples with high concentrations of titanium dioxide, the cells remained unprotected. It is noted that the mucus was adsorbed around TIO2. The lowest degree of adsorption was characterized by samples containing 0.5% TiO2, and the highest degree - with 5% TiO2. When using TiO2, more than 2.5% can completely release cells of cyanobacteria in the aqueous medium from the mucous that protects their cells, and then use other drugs for lysis. An increase in the number of dead cells was observed in cyanobacterial culture. In M. pulverea culture, the number of metabolically active cells decreases sharply with increasing concentration of TiO2. Incubation with TiO2 led to a gradual decrease in the number of metabolically active cells: from 96% (in the control sample) to about 9% in the presence of 5% TiO2. Although this culture remains relatively alive, but loses the ability to actively metabolize. It can be predicted that over time the number of M. pulverea cells will decrease sharply, which will lead to the death of the culture as a whole.

Fifteenth International Conference on Correlation Optics

With the help of optical methods of fish functional state diagnostics the possibility of using DO... more With the help of optical methods of fish functional state diagnostics the possibility of using DON-1R, a drug that contains γ–crotonolactone, in recirculating aquaculture system was proven. It was stated that adding DON-1R to granulated feed while feeding sturgeon fish stimulates the raise in mass accumulating velocity. Moreover, microscopical analysis of histological cuts and detailed analysis of their photomicrographs with the use of various approaches in contrasting images showed that feeding with feed with DON-1R is accompanied with reactive changes to the liver. In particular, fish that received the tested drug had the inequable liver cytoarchitectonics, chaotic hepatocytes placement, radial character of liver channels and undefined sinusoids. Hepatocytes of experimental fish had large hyperchromatic nuclei with 4,5 μm diameter with multiple tiny defined nucleoli. Control fish’s nuclei were 1,5 times smaller, had even coloring and 1-2 nucleoli. Moreover, fish from experimental group had signs of partial acidophilic coagulation, such as occurrence of homogenous pink lumps in cytoplasm (Mallory bodies). It is worth noting that the application of DON-1R does not lead to deep destructive changes in liver structure, such as necrosis of fibrosis. Taking all of the above into consideration, DON-1R is the most effective for growth stimulation if applied for a short period of time for commercial growing. Optimal period of use is 20 days.

The dataset consists of records of protected plant and fungi species published in a series of pub... more The dataset consists of records of protected plant and fungi species published in a series of publications specially prepared by the Institute of Zoology: 1) Materials for the 4th edition of the Red Book of Ukraine. Fauna / Series: «Conservation Biology in Ukraine». – Vol. 6, Т. 1. – Kyiv, I. I. Schmalhausen institute of zoology NAS of Ukraine, 2018. – 438 p. 2) Materials for the 4th edition of the Red Book of Ukraine. Fauna / Series: «Conservation Biology in Ukraine». – Vol. 6, Т. 2. – Kyiv, I. I. Schmalhausen institute of zoology NAS of Ukraine, 2018. – 450 p. 3) Materials for the 4th edition of the Red Book of Ukraine. Fauna (Series: «Conservation Biology in Ukraine». – Vol. 7, T. 3). - Kyiv, 2019. - 416 p.

The article presents the results of a study of the regularities of the scattering of polarized li... more The article presents the results of a study of the regularities of the scattering of polarized light by fish mucus and fish scales in order to identify matrix elements that are most sensitive to changes in the conformation, shape and size of protein macromolecules of fish mucus, and to identify the structural features of fish scales.

Biolohichni systemy, Dec 23, 2020

<jats:p>We studied the possibility of using low frequency laser emission to improve product... more <jats:p>We studied the possibility of using low frequency laser emission to improve production properties of feed hydrobionts, in particular microalgae Desmodesmus armatus and Daphnia magna, which are highly applied in aquaculture. It was shown that low-frequency laser emission with the wavelength 650 nm has a positive influence on production properties of both phyto- and zooplankton. In 24 hours the biomass of irradiated D. armatus was 25% larger than non-irradiated one. Meanwhile, application λ 420 and 530 nm did not show credible accumulation of microalgae biomass. If it comes to daphnia, positive dynamic in population density enlarging was observed only since 5-7th day of cultivation, depending on emission exposition. Differences in low-frequency laser emission with λ 420 and 530 efficacy on D. magna production properties were observed while using different exposition. Thus, maximal culture density was observed when λ 420 nm for 90 s and λ 650 nm for 60 s were applied. Longer irradiation with red laser did not have positive effect. It was established that in daphnia tissues, which were influenced by λ 420 nm laser emission, TBA-active products were accumulating more. Meanwhile, for daphnias, which were irradiated with red laser, TBA amount was not different from the control group one. Catalase activity increased while applying λ 650 nm laser emission. On the other hand, λ 420 nm did not cause credible increasing of catalase activity in comparison with control group. Taking received results about photobiological effects of low-frequency laser emission on D. armatus and D. magna into consideration, both biomasses enlargement during co-cultivation can be obtained if wavelength 650 nm is used.</jats:p>

Archives of Polish Fisheries, 2018

The first aim of the study was to determine the impact the commercial product S.presso (INVE Aqua... more The first aim of the study was to determine the impact the commercial product S.presso (INVE Aquaculture, Belgium) had on enriching the proximate composition of brine shrimp (Artemia sp.). The second aim of the study was to develop a better enrichment protocol for preparing enriched brine shrimp larvae. Two different enrichment protocols were investigated; the supplement was administered in either one (0.5 g L-1) or two equal (0.25 g L-1) doses. Using S.presso to enrich brine shrimp nauplii with polyunsaturated fatty acids resulted in higher levels of total lipids and higher quantities of docosahexaenoic acid DHA and eicosapentaenoic acid EPA deposition. Additionally, the enrichment supplement did not lead to overall lowered protein content values caused by increased hydrolytic activity that likely facilitates digestion in larval fish as they shift to exogenous feeding.

International Letters of Natural Sciences, 2016

The results of using the waste water from recirculating aquaculture system for phyto- and zooplan... more The results of using the waste water from recirculating aquaculture system for phyto- and zooplankton cultivation are presented in the article. The physico-chemical parameters of the aquaculture system’s waste water and comparative culture media were recorded during hydrobionts cultivation. The indicators of productivity and the biochemical composition of investigated cultures were determined. The biochemical analysis of studied cultures included the investigations of the content of total proteins, lipids, carbohydrates, carotenoids and photosynthetic pigments. Using aquaculture system’s waste water as a medium for the cultivation of phyto- and zooplankton can significantly reduce the cost of technology for live feed biomass increase in the aquaculture industries.

Fisheries & Aquatic Life, 2020

This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae bioma... more This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae biomass and then using this method to correct the nutritional composition of Artemia. Accordingly, the influence of three species of microalgal monocultures was analyzed (2 freshwater – Desmodesmus armatus (Chod.) Hegew. and Chlorella vulgaris Veijerinck; one halophilous – Dunaliella viridis Teodor.). The algal monocultures were applied once in a quantity of 2-3 × 106 cells × l−1 for each 200,000 Artemia individuals hatched. The control group Artemia did not receive algae. The enrichment process lasted 24 h, and control measurements were performed every 6 h. The survival of Artemia nauplii, their proteolytic activity, and the content of proteins, lipids, and carotenoids were analyzed. The choice of algae species for Artemia enrichment was guided by the size of the algal cells and their biochemical composition. Selected algae contained about 50% protein, 20% lipid, and 12 mg of carotenoids per...

Fisheries & Aquatic Life, 2020

Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of esse... more Freshwater zooplankton, which is commonly used as a starting fish feed, has a low content of essential compounds such as carotenoids. The possibility of increasing the productivity of carotenogenic yeast, Rhodotorula glutinis, for further bioencapsulation of zooplankton Daphnia magna is shown. An association of a UV irradiated yeast strain with lactic acid bacteria was cultivated for this purpose. This permitted intensifying the carotenogenesis of yeast, and the content of â-carotene increased by 1.7 times, and that of torularodine by 2.3 times compared with the native monoculture. The use of the association of microorganisms as a feed substrate in the cultivation of Daphnia magna provided both an increase in the carotenoid content in the investigated crustaceans more than 8 times, and their biomass by 2.5 times compared with the standard feed of Saccharomyces cerevisiae.

Biolohichni systemy, 2020

The anthibiotics use in aquaculture these days is severely restricted by European standarts of ma... more The anthibiotics use in aquaculture these days is severely restricted by European standarts of marketable fish products quality. According to this, one of the most efficient alternatives are probiotics. So the search of applicable probiotic microorganisms that can be applied in aquaculture is relevant. On the other side, probiotics introduction into the fish body is usually accomplished in composition with dry feed as a biofilm. But a lot of fish larvae are not capable of consuming dry artificial feed due to numerous reasons. For their transmission for endogenic nourishment from exogenic live feeds are used in aquaculture, which have a higher level of digestibility compared to granulated feed. One more advantage of live feed is that they can be used as vectors to provide the targeted delivery of probiotics into fish body. In this work Lactobacillus casei IMV 7280 was firsly tested for the needs of aquaculture. It is shown that the usage of this probiotic during the growing of carp w...

Aquaculture Research, 2018

Successful breeding of fish species in aquaculture depends on several factors, among which the te... more Successful breeding of fish species in aquaculture depends on several factors, among which the temperature and feed are the most significant ones. This study was designed to evaluate the effect of temperature in the range of 14–18°C on the rate of embryogenesis and duration of larva period and to estimate the efficiency of Artemia nauplii enriched with PUFAs in growing sturgeon larvae. The temperature of 16°C is the most suitable for both egg incubation and Atlantic sturgeon prelarvae maintenance under aquaculture conditions. Even minor temperature fluctuations of 1 degree up or down leads to increased loss both of eggs and prelarvae. Increased temperature shortens the incubation period but has a lesser impact on the duration of prelarvae onset of external feeding. The technology of Artemia nauplii bioencapsulation with a PUFA‐containing supplement in A. oxyrinchus rearing increases sturgeon larvae weight by 1.5 times at a constant survival dynamic.

The research work is meant to investigate the possibility of using the zeolite-containing tuffs i... more The research work is meant to investigate the possibility of using the zeolite-containing tuffs in the technology of live fish feed cultivation by example of Cladocera. It is shown that the use of natural and treated at the temperature of 150°C basaltic tuffs from „Polytske-2” basalt deposit during Moina macrocopa cultivation leads to an increase of the culture density by 2 and 2.3 times, respectively. In the experiment with Simocephalus vetulus the increase of culture density has not been found, however basaltic tuffs allow to stabilize the culture growth and slow its extinction rate. Besides, it is observed the effective purification of cultural medium of dissolved nitrogen forms probably by adsorption. The use of basaltic tuffs, calcined at a temperature of 1000 °C, leads to the death of M. macrocopa and S. vetulus cultures in 6 days. This circumstance makes the use of calcined tuffs impractical.

Fisheries & Aquatic Life, 2020

This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae bioma... more This study focused on testing Artemia sp. zooplankton saturation with freshwater microalgae biomass and then using this method to correct the nutritional composition of Artemia. Accordingly, the influence of three species of microalgal monocultures was analyzed (2 freshwater – Desmodesmus armatus (Chod.) Hegew. and Chlorella vulgaris Veijerinck; one halophilous – Dunaliella viridis Teodor.). The algal monocultures were applied once in a quantity of 2-3 × 106 cells × l−1 for each 200,000 Artemia individuals hatched. The control group Artemia did not receive algae. The enrichment process lasted 24 h, and control measurements were performed every 6 h. The survival of Artemia nauplii, their proteolytic activity, and the content of proteins, lipids, and carotenoids were analyzed. The choice of algae species for Artemia enrichment was guided by the size of the algal cells and their biochemical composition. Selected algae contained about 50% protein, 20% lipid, and 12 mg of carotenoids per...

The maintenance of live feed in automatic feeders is accompanied by fasting of fodder organisms, ... more The maintenance of live feed in automatic feeders is accompanied by fasting of fodder organisms, resultedin a lose of their nutritional value and even death. The way out of this situation can be the joint maintenance of Artemia nauplii and their fodder objects – microalgae. Accordingly, an influence of three microalgae monocultures (Desmodesmus armatus, Chlorella vulgaris, Dunaliella salina) and the AlgaMac Protein Plus drug on survival of Artemia nauplii was analyzed. It was shown that the lowest mortality rates of brine shrimp were observed when it was co-cultivated with monoculture of Chlorella. The use of the AlgaMac Protein Plus algal drug provided a significant reduction in the mortality rate of Artemia nauplii at 12 hours of cultivation. Cell size of studied algal species is a key point for their using as a fodder object for brine shrimp. Maximum diameter of Chlorella cells reaches 10 μm. Cells of D. armatus are single nucleated autospores with coccoid shape. Their size is 10...

International Letters of Natural Sciences

The nutritional composition and amino acid profile ofMoina macrocopawere studied using different ... more The nutritional composition and amino acid profile ofMoina macrocopawere studied using different types of yeast (Saccharomyces cerevisiaeandRhodotorula glutinis) as fodder substrates. The effective accumulation of carotenoids inMoina macrocopaduringR. glutinisyeast application was not accompanied by deterioration in the nutritional value of zooplankton. The content of total proteins and total lipids inMoinagrown onS. cerevisiaeandR. glutiniswas not significantly different. However, the use ofR. glutinisin the cultivation ofM. macrocopaled to the change in the ratio of proteinogenic amino acids in the studied cladocerans. In particular, the share of methionine, leucine and isoleucine significantly increased. It allowed to enhance the quality of protein in the fodder zooplankton, that is especially important in the feeding of fish fry.