SL Kothari | University of Rajasthan, Jaipur (original) (raw)

Papers by SL Kothari

Journal of Plant Physiology, 1984

The immature unpollinated disc florets showed differentiation of a large number of shoot primordi... more The immature unpollinated disc florets showed differentiation of a large number of shoot primordia on MS (Murashige and Skoog, 1962) basal medium supplemented with BAP (5 mg .1-1 ) + IAA (3 mg '1-1 ). These shoot buds were further grown on a medium with BAP (3 mg .1-1 ) + IAA (5 mg ,1-1 ) + GA3 (0.5 mg ,1-1 ) where they grew to a well developed shoot system. Complete plantlets were obtained by subculturing the shoots on a medium with IBA (0.5 mg .1-1 ) + GA3 (0.5 mg ,1-1 ). Plant regeneration from callus tissue could be maintained for two years by subculturing the callus together with developing shoot buds.

Journal of Plant Biochemistry and Biotechnology, 2004

The effect of various concentrations of CuS0 4 on the induction and regeneration of embryogenic c... more The effect of various concentrations of CuS0 4 on the induction and regeneration of embryogenic callus from immature embryos of wheat was investigated. Immature embryos of wheat cvs C-306 and R-3777 were cultured on MS medium supplemented with 2,4-0 (11.3 IJM) and different levels of cupric sulphate, i.e. 0, 0.1 (MS level), 0.5, 1 and 5 IJM. Relatively high induction frequency of callus was obtained on MSmedium supplemented with 2,4-0 (11.3IJM) and 0.51JM CuS0 4 • The compact, nodular, embryogenic callus was maintained on the medium having 2,4-0 (11.3 IJ M) and proline (86.8 IJ M) by regular subculturing. Plant regeneration from the embryogenic callus occurred on MS medium supplemented with NAA (1.07 IJM) and SAP (44.4 IJM). Regenerated plantlets were rooted on MS medium supplemented with IAA (2.85 IJM). The average number of regenerated plantlets produced from primary callus induced on 2,4-0 (11.3 IJM) and 5x CuS0 4 was significantly higher.

Plant Biotechnology, 2005

Millets are small seeded grasses grown for food, feed or forage and cultivated mostly in less dev... more Millets are small seeded grasses grown for food, feed or forage and cultivated mostly in less developed countries in poor soil and dry conditions. There are at least 10 genera and 14 species of millets belonging to the Poaceae (Gramineae) family. Tissue culture and plant regeneration occurring through different morphogenic pathways have been reported in great detail in millets. Gene transfer has been attempted using various methods, but so far transgenic plants have been developed only in Pearl millet and Bahiagrass. Not much work has been done on transgenesis in other millets. This is primarily because they have less economic value and are cultivated in poor countries, where research and development are also poor. In the present review we have attempted to provide available information on millet tissue culture and genetic transformation. We have underlined the importance of transgenesis in millet improvement and the role that biotechnology can play in the improvement of these crops grown in a variety of harsh conditions.

Advances in Intelligent Systems and Computing, 2016

Journal of Bionanoscience, 2015

ABSTRACT Common carbohydrates of distinct classes (mono, di and polysaccharides) were examined to... more ABSTRACT Common carbohydrates of distinct classes (mono, di and polysaccharides) were examined to synthesize silver nanoparticles (Ag NPs) and a comparison was made about the efficacy towards synthesizing the Ag NPs and properties of the later. Six monosaccharides (L-rhamnose, xylose, arabinose, glucose, fructose and galactose), three disaccharides (sucrose, maltose and cellobiose) and two polysaccharides (starch and agar) were tested as reducing agents of silver ions. The formation and stabilization of Ag NPs was assessed with UV-Vis, PL and FT-IR spectroscopy, SEM and EDAX measurements. Distinctive colors with varying λmax on UV-Vis and PL spectra were observed in the aqueous solutions of the Ag NPs produced by different carbohydrates. FT-IR spectroscopy revealed the attachment of carbohydrate moieties to Ag NPs. On SEM and EDAX measurements, agar exhibited the best results by producing the smallest Ag NPs (30–50 nm) with high yield, which makes it suitable for industrial production of Ag NPs.

International Journal of Genomics, 2014

Physiology and molecular biology of plants : an international journal of functional plant biology, 2014

The present study reports an efficient in vitro micropropagation protocol for a medicinally impor... more The present study reports an efficient in vitro micropropagation protocol for a medicinally important tree, Terminalia bellerica Roxb. from nodal segments of a 30 years old tree. Nodal segments taken from the mature tree in March-April and cultured on half strength MS medium gave the best shoot bud proliferation response. Combinations of serial transfer technique (ST) and incorporation of antioxidants (AO) [polyvinylpyrrolidone, PVP (50 mg l(-1)) + ascorbic acid (100 mg l(-1)) + citric acid (10 mg l(-1))] in the culture medium aided to minimize browning and improve explant survival during shoot bud induction. Highest multiplication of shoots was achieved on medium supplemented with 6-benzyladenine (BA, 8.8 μM) and α-naphthalene acetic acid (NAA, 2.6 μM) in addition to antioxidants. Shoot elongation was obtained on MS medium containing BA (4.4 μM) + phloroglucinol (PG, 3.9 μM). Elongated shoots were transferred to half strength MS medium containing indole-3-butyric acid (IBA, 2.5 μM)...

Methods in molecular biology (Clifton, N.J.), 2010

In this chapter, we describe multiplication of the superior and elite tree of Crataeva adansonii ... more In this chapter, we describe multiplication of the superior and elite tree of Crataeva adansonii using plant tissue culture techniques. An ornamental and avenue tree, it is not available in abundance because of poor seed germination and seedling establishment. It reproduces in nature by root suckers, but that restricts its distribution to very limited areas. Efficient procedures are outlined for plant regeneration through direct shoot bud formation, indirect organogenesis, and somatic embryogenesis through callus formation. Different explants were utilized for separate pathways of regeneration. Murashige and Skoog's (MS) medium containing 3 mg/L BA and 0.05-0.1 mg/L NAA is most effective in direct induction of axillary buds from nodal explants and shoot tips. Adventitious shoots developed from leaves on MS medium containing 3 mg/L BA and 0.1 mg/L NAA. De novo shoots were obtained from the anthers on MS medium supplemented with 3 mg/L BA. Somatic embryos developed on half strengt...

Summary A highly efficient protocol for callus induction and plant regeneration in Sorghum bicol... more Summary A highly efficient protocol for callus induction and plant regeneration in Sorghum bicolor was developed by varying the concentrations of copper (0.1, 0.3, 0.5, 0.7, 1, 1.5, 2.5 μM) in Murashige and Skoog (MS) medium. The mature embryos of Sorghum bicolor were cultured on MS medium containing 2,4-dichlorophenoxyacetic acid (9μM), kinetin (2.3 μM), and 3% (w/v) sucrose for embryogenic callus induction. Plant regeneration from this callus occurred on MS medium containing kinetin (9.2 μM) and indole-3-acetic acid (2.85 μM). A much greater response was noted on these media with higher levels of copper. Frequency of plant regeneration and number of regenerants dramatically increased with an optimal amount of copper (2 μM) in the MS medium. Rooting of the regenerated shoots readily occurred on half-strength MS medium supplemented with α-naphthaleneacetic acid (10.7 μM) and 3% (w/v) sucrose. Well-developed plantlets were transferred to the field where 100% survival and normal seed setting was noted.

Indian journal of experimental biology, 2010

Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemic... more Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are often toxic and flammable. In the present study, the spore crystal mixture of Bacillus thuringiensis was used for the synthesis of silver nanoparticles. Nanoparticles were characterized using UV-Vis absorption spectroscopy, XRD and TEM. X-ray diffraction and TEM analysis showed the average particle size of 15 nm and mixed (cubic and hexagonal) structure. This is for the first time that any bacterial spore crystal mixture was used for the synthesis of nanoparticles. Further, these biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogenic bacteria.

Scientia Horticulturae, 2009

Incorporation of a range of higher concentrations of CuSO 4 Á5H 2 O in MS medium . A revised medi... more Incorporation of a range of higher concentrations of CuSO 4 Á5H 2 O in MS medium . A revised medium for rapid growth and bioassay with 240 tobacco tissue cultures. Physiol. Plant 15, 473-497] significantly enhanced direct shoot bud induction and proliferation from cultured leaf and nodal explants taken from mature plants of Stevia rebaudiana. Shoot bud induction medium was supplemented with BAP (2.2 mM) and IAA (2.8 mM). When the concentration of CuSO 4 Á5H 2 O in the induction medium was raised to 0.5 mM (five times the MS level, i.e. 0.1 mM) there was significant increase in percentage response along with increase in shoot bud number per explant. The shoots were healthy, well developed with dark green broader leaves. There was remarkable increase in total biomass and chlorophyll content at increased (0.5 mM) copper level in the medium. During proliferation stage also presence of high copper levels in the medium favoured increase in shoot bud number per explant. ß

Physiology and Molecular Biology of Plants, 2012

Trees, 2014

Key message Pithecellobium dulce has a huge repository of morphological and genetic diversity wit... more Key message Pithecellobium dulce has a huge repository of morphological and genetic diversity within the species which has been documented for further tree research and breeding programmes. Abstract A total of 40 accessions of a multipurpose leguminous tree, Pithecellobium dulce, differing in morphological and phenological characteristics was collected from different locations in Rajasthan and genetic diversity assessed using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of 60 RAPD primers, 43 decamer primers produced 311 scorable bands of which 223 bands were polymorphic. Polymorphic banding patterns with the number of amplified fragments varied from 1 (OPA-12 and OPF-17) to 12 (OPF-04, OPT-07, . Percent polymorphism ranged from 33.3 % (OPF-07, OPA-20, OPT-05) to 89 % (OPA-13) with an average of 64.34 %. Out of the 20 ISSR primers screened, 8 primers produced 77 amplification products of which 55 were polymorphic. The number of bands amplified per primer varied between 6 (UBC-820) and 13 (UBC-808 and UBC-816) with average band size between 300 and 4,500 bp. Percent polymorphism ranged from 38 % (UBC-817) to 100 % (UBC-811) with an average of 71 %. Dendrogram constructed on the basis of combined RAPD and ISSR markers separated the accessions into five distinct clusters at 53 % variation with Jaccard's similarity coefficient ranging from minimum similarity (0.35) between Karoli (K) and Malviya Nagar, Jaipur (MN1) to maximum similarity (0.81) between two populations of Kota (K1 and K2). The matrices for two markers, RAPD and ISSR, were also compared using Mantel's test. The correlation value between the matrices is high (r = 0.83) indicating good correlation between the two molecular marker systems. The study indicated a high level of morphological and genetic diversity in P. dulce.

Nanotechnology and Plant Sciences, 2015

Nanotechnology is a very promising area of research which involves the production of nanomaterial... more Nanotechnology is a very promising area of research which involves the production of nanomaterials as the basic strategy. Although artificial synthesis of nanomaterials were initiated by using chemical and physical approaches, but recently the biological synthesis methods are being widely used as ecofriendly alternatives. Plant-based synthesis of nanomaterials is better because of its ease of handling, rapidity, and cost-effective nature along with environmental friendliness. A wide range of applications of silver nanoparticles (AgNPs) creates a focal point for attention of researchers. In view of published studies, in this chapter, we critically assess the role of plants in the synthesis of AgNPs, the characterization methods, applications of biologically synthesized AgNPs in various fields and future perspectives.

Plant Cell Reports, 1993

In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innox... more In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in culture. Embryos differentiated and matured in the liquid medium itself as also evidenced by histological observation. Embryos germinated to form plantlets on semisolid MS medium without growth substances. The regenerated plants had haploid number of chromosomes.

Theoretical and Applied Genetics, 1986

Protoplasts from different Daucus carota L. cell strains carrying resistance to glyphosate, 5-met... more Protoplasts from different Daucus carota L. cell strains carrying resistance to glyphosate, 5-methyltryptophan, sodium selenate or selenocystine were fused in three combinations using dextran. Clones were selected for both of the resistances carried by the individual parental strains in medium with both inhibitors. No doubly resistant colonies formed from unfused controls or from protoplasts from each individual parental strain alone. Suspension cultures from the selected clones contained predominantly the additive chromosome numbers of the parental strains. Apparently the four resistances used are expressed dominantly in fusion hybrids. Analysis of mitochondrial DNA showed that recombination occurred in one fusion combination since the mitochondrial DNA in the hybrid cells was different from that of either parent as shown by restriction endonuclease fragment analysis. Mitochondrial DNA in the other two somatic hybrid combinations was parental. Thus, a dominant, nuclear resistance marker system has been developed to select efficiently for somatic hybrids in which mitochondrial DNA recombination can be studied.

National Academy Science Letters, 2012

Your article is protected by copyright and all rights are held exclusively by The National Academ... more Your article is protected by copyright and all rights are held exclusively by The National Academy of Sciences, India. This e-offprint is for personal use only and shall not be selfarchived in electronic repositories. If you wish to self-archive your work, please use the accepted author's version for posting to your own website or your institution's repository. You may further deposit the accepted author's version on a funder's repository at a funder's request, provided it is not made publicly available until 12 months after publication.

Plant Cell, Tissue and Organ Culture (PCTOC), 2011

A micropropagation system through leaf explant culture has been developed for Withania coagulans.... more A micropropagation system through leaf explant culture has been developed for Withania coagulans. Shoot bud proliferation occurred through both adventitious and de novo routes depending on the hormonal regime of the culture medium. Green compact nodular organogenic callus developed on Murashige and Skoog (MS) medium supplemented with 2.3 lM kinetin (Kn) and lower levels of 6-benzyladenine (BA) (13.3 lM) while multiple adventitious shoot bud differentiation occurred on medium fortified with 2.3 lM kinetin (Kn) and higher levels of BA (22.2 lM). Shoot buds were transferred to proliferation medium containing 2.2 lM BA, 2.3 lM Kn, and 3.9 lM phloroglucinol (PG) for further growth and development of shoot system. Elongated shoots were rooted using a two-step procedure involving pulse treatment of 7 days in a medium containing 71.6 lM choline chloride (CC) and 3.9 lM PG and then transferred to rooting medium containing MS, 1.2 lM IBA, 3.6 lM PAA, and 14.3 lM CC for 3 weeks. Well-rooted plants were transferred to a greenhouse for hardening and further growth. Random amplification of polymorphic DNA (RAPD) showed monomorphic bands in all the plants thereby confirming clonality of the regenerants. Thin layer chromatography (TLC) showed the presence of withanolides in the regenerated plants. Quantification through reverse-phase HPLC revealed increased concentration of withanolides in the regenerated plants compared to the field-grown mother plant. Accumulation of withaferin A and withanolide A increased up to twofold and that of withanone up to tenfold. Direct regeneration via leaf explants will be useful for Agrobacterium-mediated genetic transformation, and will facilitate pathway manipulation using metabolic engineering for bioactive withanolides.