Sukanya Phuengjayaem | Chulalongkorn University (original) (raw)

Papers by Sukanya Phuengjayaem

International journal of pharma medicine and biological sciences, 2020

Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe b... more Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe bacteria. It grew in 4% (w/v) NaCl, at a pH range of 5.0-8.0 (optimally at pH 7) and at 30-40 º C (optimally at 37 º C). Major cellular fatty acids were C16:0, C17:0 cyclo, C16:1 w9c, and C18:1 w9c of 36.33, 9.67, 13.85 and 10.74% of total component, respectively. The DNA G+C content of the type strain was 40.84 mol%. Based on the result of 16S rRNA gene sequence analysis. It was closely related to Clostridium amygdalinum BR-10 T (97.84%), Clostridium saccharolyticum WM1 T (97.76%) and Clostridium celleracrescens DSM 5628 T (97.69%). There were few researches studied on the production of succinic acid by the genus of Clostridium. Clostridium SP17-B1 was able to produce succinic acid 25.07 g/L from 40 g/L of glucose as a substrate. The application of lignocellulose as a carbon source for succinic production from this strain is under studied. 

Scienceasia, 2023

Fourteen lactic acid bacteria isolated from fermented foods and healthy animal feces in Thailand ... more Fourteen lactic acid bacteria isolated from fermented foods and healthy animal feces in Thailand were characterized for their potential as probiotics. The 16S rRNA gene sequence analyses identified them as Lactiplantibacillus, Levilactobacillus, Limosilactobacillus, Lacticaseibacillus, Campanilactobacillus, Pediococcus, and Enterococcus genera. All strains survived in simulated gastrointestinal fluid (pH 2) and bile salt solution (pH 8) at over 70% and 63%, compared with initial cell concentration, respectively. In vitro adhesion testing showed their adhesive property of over 70%, while the results of antibiotic susceptibility indicated that all strains were susceptible to amoxicillin, ampicillin, erythromycin, chloramphenicol, clindamycin, imipenem, kanamycin, norfloxacin, penicillin, tetracycline, and vancomycin. All strains exhibited antimicrobial ability against Staphylococcus aureus TISTR 1466, Listeria monocytogenes TISTR 2196, Escherichia coli TISTR 780, Salmonella Enteritidis TISTR 2202, and Salmonella Typhimurium TISTR 292. Moreover, Limosilactobacillus reuteri MF67.1 and Companilactobacillus farciminis R7-1 showed bile salt hydrolase activity. Cellfree culture supernatants of all 14 strains were screened for immunomodulating effects on Tumor Necrosis Factor alpha (TNF-α) production. Lactiplantibacillus paraplantarum R26-3 and Lacticaseibacillus zeae M2/5 showed high inhibition of TNF-α production at 34% and 29% reduction, respectively; while the other 12 strains decreased TNF-α production at various lower levels. Results suggested that all 14 strains met the general criteria of probiotics. Lac. zeae M2/5, Lac. paraplantarum R26-3, Lim. reuteri MF67.1, and Com. farciminis R7-1 were interesting candidates for further studies as anti-inflammatory (M2/5, R26-3) or cholesterol-reducing agents (MF67.1, R7-1) in in vivo animal models.

Anaerobe, Feb 1, 2020

An anaerobic, gram-positive, rod-shaped bacterium strain SP17eB1, isolated from dog saliva, was t... more An anaerobic, gram-positive, rod-shaped bacterium strain SP17eB1, isolated from dog saliva, was taxonomically characterized on the basis of phenotypic, chemotaxonomic, and genotypic characteristics. It was cultured in 4% (w/v) NaCl at a pH range of 5.0e8.0 (optimally at pH 7) and at 30 Ce40 C (optimally at 37 C). Its major cellular fatty acids are C 16:0 (36.3%), C 17:0 cyclo (9.7%), C 16:1 u9c (13.9%), and C 18:1 u9c (10.7%), and its DNA guanineecytosine content is 40.8 mol%. On the basis of the 16S rRNA gene sequence analysis, it was determined that the strain belonged to the genus Clostridium and was closely related to C. amygdalinum BR-10 T (97.8%), C. saccharolyticum WM1 T (97.8%), and C. celleracrescens DSM 5628 T (97.7%). This strain showed a low level of DNAeDNA relatedness with the closely related strains, suggesting that it is a novel species in the genus Clostridium. Recent studies have demonstrated the production of succinic acid using Clostridium strains. Strain SP17eB1 produced 25.1 ± 1.3 and 15.3 ± 1.5 g/L of succinic acid from 40 g/L of glucose and 30 g/L of hevea wood waste hydrolysate (HH), respectively, after 24 h. When detoxified HH was used as a substrate, the lag phase was reduced and cell growth was enhanced by 7 fold (OD 660 0.4e3.0) within 12 h. Detoxification using granular activated carbon may have reduced the levels of furfural and HMF without interfering with the amount of sugars in HH.

International Journal of Systematic and Evolutionary Microbiology, Feb 1, 2021

A Gram-stain-positive, catalase-negative, rod-shaped, non-motile, non-spore-forming, and facultat... more A Gram-stain-positive, catalase-negative, rod-shaped, non-motile, non-spore-forming, and facultatively anaerobic strain CRM56-3T, isolated from fermented tea leaves collected from Chiang Rai province, Thailand, was characterized based on a polyphasic approach. The strain produced dl-lactic acid heterofermentatively from glucose. It grew at 15–42 °C (optimum at 30 °C), pH 3.5–8.0 (optimum pH 6.0) and in 1–4 % (w/v) NaCl. Strain CRM56-3T contained C16:0, C19:0 cyclo ω8c, and C18:1 ω7c, and/or C18:1 ω6c as major cellular fatty acids. Based on 16S rRNA gene sequence analysis, strain CRM56-3T belongs to the genus Secundilactobacillus and was closely related to Secundilactobacillus odoratitofui DSM 19909T (99.2 %), S. collinoides JCM 1123T (98.9 %), and S. paracollinoides DSM 15502T (98.7 %). The draft genome of strain CRM56-3T contained 2681617 bp with 2413 coding sequences and DNA G+C content determined from genome sequence of 44.5 mol%. The digital DNA–DNA hybridization (dDDH) between strain CRM56-3T and S. odoratitofui DSM 19909T, S. collinoides JCM 1123T, and S. paracollinoides DSM 15502T were 19.5, 20.4, and 21.6 %, respectively. The average nucleotide identity (ANIm) and the average amino acid identity (AAI) between strain CRM56-3T and closely related strains were lower than 85.0 and 80.0 %, respectively. The strain CRM56-3T was clearly distinguished from related Secundilactobacillus species by its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity, and the draft genome analysis. Therefore, the strain represents a novel species of the genus Secundilactobacillus, for which the name of Secundilactobacillus folii sp. nov. is proposed. The type strain is CRM56-3T (=JCM 34223T=LMG 31663T=TISTR 2851T).

Sugar Tech, Jun 29, 2013

This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The b... more This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The biomass loading was 10 % w/v and the pretreatment parameters, concentration of sulfuric acid (0–3 % v/v), temperatures (120–190 °C) and residence times (10–30 min) were investigated. The monomeric sugars (glucose, xylose, galactose, arabinose, mannose) in hydrolyzate were analyzed using high performance liquid chromatography. The maximum yield of glucose and xylose from sweet sorghum straw was 0.234 g glucose/g dry substrate and 0.208 g xylose/g dry substrate, respectively, at the pretreatment condition: 120 °C, 3 % H2SO4 for 10 min. In this case, a total of 50.04 % of glucan and 76.41 % of xylan were converted to glucose and xylose, respectively.

This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric aci... more This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric acid pretreatment of sweet sorghum straw followed by enzymatic saccharification of a commercial cellulase (Celluclast 1.5, Novozyme). The sweet sorghum straw was mixed with 3% dilute sulfuric acid with 10%w/v of solid loading and then, pretreated at various temperatures (120-190°C) for 10 min of pretreated times. The maximum yields of glucose and xylose from sweet sorghum straw were 0.234 g/g dry substrate and 0.208 g/g dry substrate, respectively, at the pretreatment condition: 120 ºC, 3%H 2 SO 4 for 10 min. In this case, a total of 50.04% of glucan and 76.41% of xylan were converted to glucose and xylose, respectively. The saccharification conditions were 1.0-7.0% of the acid pretreated sweet sorghum straw, 15-35 FPU/g-substrate of cellulase, pH from 3 to 7 and temperature from 30 to 50 o C. A maximum saccharification yield of glucose was 0.366 g/g dry substrate at the optimal condition: 1.0-2.5% of the acid pretreated sweet sorghum straw, 30 FPU/g-substrate of cellulase, pH in the range 3-5, temperature in the range 30-50 o C and 96 h of incubation time. Furthermore, the saccharification conditions were optimized using a statistical analysis (Response surface methodology).

Functional & Integrative Genomics

International Journal of Pharma Medicine and Biological Sciences

Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe b... more Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe bacteria. It grew in 4% (w/v) NaCl, at a pH range of 5.0-8.0 (optimally at pH 7) and at 30-40 º C (optimally at 37 º C). Major cellular fatty acids were C16:0, C17:0 cyclo, C16:1 w9c, and C18:1 w9c of 36.33, 9.67, 13.85 and 10.74% of total component, respectively. The DNA G+C content of the type strain was 40.84 mol%. Based on the result of 16S rRNA gene sequence analysis. It was closely related to Clostridium amygdalinum BR-10 T (97.84%), Clostridium saccharolyticum WM1 T (97.76%) and Clostridium celleracrescens DSM 5628 T (97.69%). There were few researches studied on the production of succinic acid by the genus of Clostridium. Clostridium SP17-B1 was able to produce succinic acid 25.07 g/L from 40 g/L of glucose as a substrate. The application of lignocellulose as a carbon source for succinic production from this strain is under studied. 

Science Faculty of Chiang Mai University, 2018

Sugar Tech, 2013

This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The b... more This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The biomass loading was 10 % w/v and the pretreatment parameters, concentration of sulfuric acid (0–3 % v/v), temperatures (120–190 °C) and residence times (10–30 min) were investigated. The monomeric sugars (glucose, xylose, galactose, arabinose, mannose) in hydrolyzate were analyzed using high performance liquid chromatography. The maximum yield of glucose and xylose from sweet sorghum straw was 0.234 g glucose/g dry substrate and 0.208 g xylose/g dry substrate, respectively, at the pretreatment condition: 120 °C, 3 % H2SO4 for 10 min. In this case, a total of 50.04 % of glucan and 76.41 % of xylan were converted to glucose and xylose, respectively.

Journal of Agricultural Science, 2014

This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maxi... more This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maximum cellulase activity of 0.897 U/ml was obtained after 5 days of cultivation using 20 g/l cellobiose as a carbon source and 2 g/l ammonium sulfate supplemented with 0.3 g/l urea as nitrogen sources. Enzymatic saccharification of acid-pretreated sweet sorghum straw (SSS) using in house cellulase was optimized using Response Surface Methodology (RSM), variable five-code-level, four-factor; 1.0-7.0% w/v acid-pretreated SSS, 15-35 FPU/g dry substrate of cellulase enzyme, pH 3 to 7 and temperatures 30 to 70 °C. The optimal conditions were 1% w/v acid-pretreated SSS, 25 FPU/g dry substrate of cellulase, pH 5, 50 °C and 72 h cultivation. A maximal glucose yield of 0.440 g/g dry substrate was obtained.

International Journal of Systematic and Evolutionary Microbiology

A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, design... more A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, designed strain CPB3-1T, was isolated from tree bark. This homofermentative strain produced dl-lactic acid from glucose. It grew at 20–45 °C, pH 4.0–9.5 and in 0-3.0 % (w/v) NaCl. It contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant component. The major fatty acid was anteiso-C17 : 0. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown lipid. Based on the results of 16S rRNA gene sequence analysis, strain CPB3-1T belonged to the genus Sporolactobacillus and was closely related to Sporolactobacillus kofuensis DSM 11701T and Sporolactobacillus spathodeae BK117-1T (both 96.7 % similarity), Sporolactobacillus inulinus NRIC 1133T and Sporolactobacillus terrae DSM 11697T (both 96.6 % similarity), and Sporolactobacillus shoreicorticis MK21-7T, Sporolactobacillus l...

Fermentation

Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-... more Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-2, isolated from Calotropis gigantea, showed potential astaxanthin production. In this study, strain CP72-2 was identified as a putative new species in the genus Rhodotorula based on the 26S rRNA gene sequence (98% identity). It was first used as the microbial source for producing astaxanthin. Strain CP72-2 was screened for its astaxanthin production and was identified and quantified by High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-MS), and UV-Vis spectrophotometer. After a screening of astaxanthin production, various carbon sources, pH, temperature, and incubation period were evaluated for their effect on the astaxanthin production of strain CP72-2. Among the several experimental factors, the most efficient conditions for astaxanthin production were glucose (50 g/L), pH 4.5, 25 °C, and three days of cultivation. The assembly genome of strain ...

Biotechnology letters, 2021

OBJECTIVES This study aimed to screen, characterize, and annotate the genome along with the compa... more OBJECTIVES This study aimed to screen, characterize, and annotate the genome along with the comparison of GABA synthesis genes presented in lactic acid bacteria (LAB). RESULTS Thirty-five LAB isolates from fermented foods were screened for GABA production using thin-layer chromatography (TLC). Fifteen isolates produced GABA ranging from 0.07 to 22.94 g/L. Based on their GTG5 profiles, phenotypic, and genotypic characteristics, isolates LSI1-1, LSI1-5, LSI2-1, LSI2-2, LSI2-3, LSI2-5, and LSM3-1-4 were identified as Lactobacillus plantarum subsp. plantarum; isolate LSM1-4 was Lactobacillus argentoratensis; isolates CAB1-2, CAB1-5, CAB1-7, and LSI1-4 were Lactobacillus pentosus; and CAB1-1, LSM3-1-1 and LSM3-2-3 were Lactobacillus fermentum. Strains LSI2-1 and CAB1-7 from pickled vegetables were selected for genome analysis. The gadA gene (1410 bp, 470aa) was encountered in GABA production of both strains and no other glutamate decarboxylase (GAD) genes were found in the genomes when c...

Two hundred succinic acid-producing bacteria were isolated from cattle dung, soil, bovine rumen, ... more Two hundred succinic acid-producing bacteria were isolated from cattle dung, soil, bovine rumen, and tree bark collected in Thailand. Eighty-four isolates exhibited a clear zone on the screening plate. The production was analyzed qualitatively by the TLC method and quantitatively by the HPLC method. The succinic acid was in the range of 0.003-0.97 g/g glucose. Only 28 isolates were selected based on morphology, cultural and phenotypic characteristics. The isolates were divided into three groups. Twenty-three isolates in Group I were identified as Enterococcus, 2 isolates in Group II were identified as Lactobacillus, and 3 isolates in Group III were identified as Clostridium. Enterococcus durans (isolate NS15-dA1) and Enterococcus hirae (isolate NS15-bA2) were selected for further study for succinic acid production. The optimum conditions were 60 g/L of glucose, 30 g/L of yeast extract (NS15-dA1), and 30 g/L of tryptone (NS15-bA2) at pH 7.0 and 37 °C. The highest amounts of succinic ...

Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria

This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric aci... more This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric acid pretreatment of sweet sorghum straw followed by enzymatic saccharification of a commercial cellulase (Celluclast 1.5, Novozyme). The sweet sorghum straw was mixed with 3% dilute sulfuric acid with 10%w/v of solid loading and then, pretreated at various temperatures (120-190°C) for 10 min of pretreated times. The maximum yields of glucose and xylose from sweet sorghum straw were 0.234 g/g dry substrate and 0.208 g/g dry substrate, respectively, at the pretreatment condition: 120 oC, 3%H 2 SO 4 for 10 min. In this case, a total of 50.04% of glucan and 76.41% of xylan were converted to glucose and xylose, respectively. The saccharification conditions were 1.0-7.0% of the acid pretreated sweet sorghum straw, 15-35 FPU/g-substrate of cellulase, pH from 3 to 7 and temperature from 30 to 50 o C. A maximum saccharification yield of glucose was 0.366 g/g dry substrate at the optimal condition: ...

The Journal of General and Applied Microbiology

The DDBJ accession number for the 16S rRNA gene sequence of strain SL9-6 is LC496807. The GenBank... more The DDBJ accession number for the 16S rRNA gene sequence of strain SL9-6 is LC496807. The GenBank/EMBL/DDBJ accession number of the draft genome of strain SL9-6 is VSFI00000000. Genome analysis and optimization of γ γ γ γ γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials

International journal of pharma medicine and biological sciences, 2020

Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe b... more Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe bacteria. It grew in 4% (w/v) NaCl, at a pH range of 5.0-8.0 (optimally at pH 7) and at 30-40 º C (optimally at 37 º C). Major cellular fatty acids were C16:0, C17:0 cyclo, C16:1 w9c, and C18:1 w9c of 36.33, 9.67, 13.85 and 10.74% of total component, respectively. The DNA G+C content of the type strain was 40.84 mol%. Based on the result of 16S rRNA gene sequence analysis. It was closely related to Clostridium amygdalinum BR-10 T (97.84%), Clostridium saccharolyticum WM1 T (97.76%) and Clostridium celleracrescens DSM 5628 T (97.69%). There were few researches studied on the production of succinic acid by the genus of Clostridium. Clostridium SP17-B1 was able to produce succinic acid 25.07 g/L from 40 g/L of glucose as a substrate. The application of lignocellulose as a carbon source for succinic production from this strain is under studied. 

Scienceasia, 2023

Fourteen lactic acid bacteria isolated from fermented foods and healthy animal feces in Thailand ... more Fourteen lactic acid bacteria isolated from fermented foods and healthy animal feces in Thailand were characterized for their potential as probiotics. The 16S rRNA gene sequence analyses identified them as Lactiplantibacillus, Levilactobacillus, Limosilactobacillus, Lacticaseibacillus, Campanilactobacillus, Pediococcus, and Enterococcus genera. All strains survived in simulated gastrointestinal fluid (pH 2) and bile salt solution (pH 8) at over 70% and 63%, compared with initial cell concentration, respectively. In vitro adhesion testing showed their adhesive property of over 70%, while the results of antibiotic susceptibility indicated that all strains were susceptible to amoxicillin, ampicillin, erythromycin, chloramphenicol, clindamycin, imipenem, kanamycin, norfloxacin, penicillin, tetracycline, and vancomycin. All strains exhibited antimicrobial ability against Staphylococcus aureus TISTR 1466, Listeria monocytogenes TISTR 2196, Escherichia coli TISTR 780, Salmonella Enteritidis TISTR 2202, and Salmonella Typhimurium TISTR 292. Moreover, Limosilactobacillus reuteri MF67.1 and Companilactobacillus farciminis R7-1 showed bile salt hydrolase activity. Cellfree culture supernatants of all 14 strains were screened for immunomodulating effects on Tumor Necrosis Factor alpha (TNF-α) production. Lactiplantibacillus paraplantarum R26-3 and Lacticaseibacillus zeae M2/5 showed high inhibition of TNF-α production at 34% and 29% reduction, respectively; while the other 12 strains decreased TNF-α production at various lower levels. Results suggested that all 14 strains met the general criteria of probiotics. Lac. zeae M2/5, Lac. paraplantarum R26-3, Lim. reuteri MF67.1, and Com. farciminis R7-1 were interesting candidates for further studies as anti-inflammatory (M2/5, R26-3) or cholesterol-reducing agents (MF67.1, R7-1) in in vivo animal models.

Anaerobe, Feb 1, 2020

An anaerobic, gram-positive, rod-shaped bacterium strain SP17eB1, isolated from dog saliva, was t... more An anaerobic, gram-positive, rod-shaped bacterium strain SP17eB1, isolated from dog saliva, was taxonomically characterized on the basis of phenotypic, chemotaxonomic, and genotypic characteristics. It was cultured in 4% (w/v) NaCl at a pH range of 5.0e8.0 (optimally at pH 7) and at 30 Ce40 C (optimally at 37 C). Its major cellular fatty acids are C 16:0 (36.3%), C 17:0 cyclo (9.7%), C 16:1 u9c (13.9%), and C 18:1 u9c (10.7%), and its DNA guanineecytosine content is 40.8 mol%. On the basis of the 16S rRNA gene sequence analysis, it was determined that the strain belonged to the genus Clostridium and was closely related to C. amygdalinum BR-10 T (97.8%), C. saccharolyticum WM1 T (97.8%), and C. celleracrescens DSM 5628 T (97.7%). This strain showed a low level of DNAeDNA relatedness with the closely related strains, suggesting that it is a novel species in the genus Clostridium. Recent studies have demonstrated the production of succinic acid using Clostridium strains. Strain SP17eB1 produced 25.1 ± 1.3 and 15.3 ± 1.5 g/L of succinic acid from 40 g/L of glucose and 30 g/L of hevea wood waste hydrolysate (HH), respectively, after 24 h. When detoxified HH was used as a substrate, the lag phase was reduced and cell growth was enhanced by 7 fold (OD 660 0.4e3.0) within 12 h. Detoxification using granular activated carbon may have reduced the levels of furfural and HMF without interfering with the amount of sugars in HH.

International Journal of Systematic and Evolutionary Microbiology, Feb 1, 2021

A Gram-stain-positive, catalase-negative, rod-shaped, non-motile, non-spore-forming, and facultat... more A Gram-stain-positive, catalase-negative, rod-shaped, non-motile, non-spore-forming, and facultatively anaerobic strain CRM56-3T, isolated from fermented tea leaves collected from Chiang Rai province, Thailand, was characterized based on a polyphasic approach. The strain produced dl-lactic acid heterofermentatively from glucose. It grew at 15–42 °C (optimum at 30 °C), pH 3.5–8.0 (optimum pH 6.0) and in 1–4 % (w/v) NaCl. Strain CRM56-3T contained C16:0, C19:0 cyclo ω8c, and C18:1 ω7c, and/or C18:1 ω6c as major cellular fatty acids. Based on 16S rRNA gene sequence analysis, strain CRM56-3T belongs to the genus Secundilactobacillus and was closely related to Secundilactobacillus odoratitofui DSM 19909T (99.2 %), S. collinoides JCM 1123T (98.9 %), and S. paracollinoides DSM 15502T (98.7 %). The draft genome of strain CRM56-3T contained 2681617 bp with 2413 coding sequences and DNA G+C content determined from genome sequence of 44.5 mol%. The digital DNA–DNA hybridization (dDDH) between strain CRM56-3T and S. odoratitofui DSM 19909T, S. collinoides JCM 1123T, and S. paracollinoides DSM 15502T were 19.5, 20.4, and 21.6 %, respectively. The average nucleotide identity (ANIm) and the average amino acid identity (AAI) between strain CRM56-3T and closely related strains were lower than 85.0 and 80.0 %, respectively. The strain CRM56-3T was clearly distinguished from related Secundilactobacillus species by its phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence similarity, and the draft genome analysis. Therefore, the strain represents a novel species of the genus Secundilactobacillus, for which the name of Secundilactobacillus folii sp. nov. is proposed. The type strain is CRM56-3T (=JCM 34223T=LMG 31663T=TISTR 2851T).

Sugar Tech, Jun 29, 2013

This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The b... more This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The biomass loading was 10 % w/v and the pretreatment parameters, concentration of sulfuric acid (0–3 % v/v), temperatures (120–190 °C) and residence times (10–30 min) were investigated. The monomeric sugars (glucose, xylose, galactose, arabinose, mannose) in hydrolyzate were analyzed using high performance liquid chromatography. The maximum yield of glucose and xylose from sweet sorghum straw was 0.234 g glucose/g dry substrate and 0.208 g xylose/g dry substrate, respectively, at the pretreatment condition: 120 °C, 3 % H2SO4 for 10 min. In this case, a total of 50.04 % of glucan and 76.41 % of xylan were converted to glucose and xylose, respectively.

This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric aci... more This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric acid pretreatment of sweet sorghum straw followed by enzymatic saccharification of a commercial cellulase (Celluclast 1.5, Novozyme). The sweet sorghum straw was mixed with 3% dilute sulfuric acid with 10%w/v of solid loading and then, pretreated at various temperatures (120-190°C) for 10 min of pretreated times. The maximum yields of glucose and xylose from sweet sorghum straw were 0.234 g/g dry substrate and 0.208 g/g dry substrate, respectively, at the pretreatment condition: 120 ºC, 3%H 2 SO 4 for 10 min. In this case, a total of 50.04% of glucan and 76.41% of xylan were converted to glucose and xylose, respectively. The saccharification conditions were 1.0-7.0% of the acid pretreated sweet sorghum straw, 15-35 FPU/g-substrate of cellulase, pH from 3 to 7 and temperature from 30 to 50 o C. A maximum saccharification yield of glucose was 0.366 g/g dry substrate at the optimal condition: 1.0-2.5% of the acid pretreated sweet sorghum straw, 30 FPU/g-substrate of cellulase, pH in the range 3-5, temperature in the range 30-50 o C and 96 h of incubation time. Furthermore, the saccharification conditions were optimized using a statistical analysis (Response surface methodology).

Functional & Integrative Genomics

International Journal of Pharma Medicine and Biological Sciences

Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe b... more Clostridium SP17-B1 isolated from dog saliva was Gram-positive rod-shaped, facultative anaerobe bacteria. It grew in 4% (w/v) NaCl, at a pH range of 5.0-8.0 (optimally at pH 7) and at 30-40 º C (optimally at 37 º C). Major cellular fatty acids were C16:0, C17:0 cyclo, C16:1 w9c, and C18:1 w9c of 36.33, 9.67, 13.85 and 10.74% of total component, respectively. The DNA G+C content of the type strain was 40.84 mol%. Based on the result of 16S rRNA gene sequence analysis. It was closely related to Clostridium amygdalinum BR-10 T (97.84%), Clostridium saccharolyticum WM1 T (97.76%) and Clostridium celleracrescens DSM 5628 T (97.69%). There were few researches studied on the production of succinic acid by the genus of Clostridium. Clostridium SP17-B1 was able to produce succinic acid 25.07 g/L from 40 g/L of glucose as a substrate. The application of lignocellulose as a carbon source for succinic production from this strain is under studied. 

Science Faculty of Chiang Mai University, 2018

Sugar Tech, 2013

This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The b... more This study was focused on hydrolysis of sweet sorghum straw using dilute acid pretreatment. The biomass loading was 10 % w/v and the pretreatment parameters, concentration of sulfuric acid (0–3 % v/v), temperatures (120–190 °C) and residence times (10–30 min) were investigated. The monomeric sugars (glucose, xylose, galactose, arabinose, mannose) in hydrolyzate were analyzed using high performance liquid chromatography. The maximum yield of glucose and xylose from sweet sorghum straw was 0.234 g glucose/g dry substrate and 0.208 g xylose/g dry substrate, respectively, at the pretreatment condition: 120 °C, 3 % H2SO4 for 10 min. In this case, a total of 50.04 % of glucan and 76.41 % of xylan were converted to glucose and xylose, respectively.

Journal of Agricultural Science, 2014

This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maxi... more This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maximum cellulase activity of 0.897 U/ml was obtained after 5 days of cultivation using 20 g/l cellobiose as a carbon source and 2 g/l ammonium sulfate supplemented with 0.3 g/l urea as nitrogen sources. Enzymatic saccharification of acid-pretreated sweet sorghum straw (SSS) using in house cellulase was optimized using Response Surface Methodology (RSM), variable five-code-level, four-factor; 1.0-7.0% w/v acid-pretreated SSS, 15-35 FPU/g dry substrate of cellulase enzyme, pH 3 to 7 and temperatures 30 to 70 °C. The optimal conditions were 1% w/v acid-pretreated SSS, 25 FPU/g dry substrate of cellulase, pH 5, 50 °C and 72 h cultivation. A maximal glucose yield of 0.440 g/g dry substrate was obtained.

International Journal of Systematic and Evolutionary Microbiology

A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, design... more A Gram-stain-positive, facultatively anaerobic and endospore-forming rod-shaped bacterium, designed strain CPB3-1T, was isolated from tree bark. This homofermentative strain produced dl-lactic acid from glucose. It grew at 20–45 °C, pH 4.0–9.5 and in 0-3.0 % (w/v) NaCl. It contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant component. The major fatty acid was anteiso-C17 : 0. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unknown phospholipid and an unknown lipid. Based on the results of 16S rRNA gene sequence analysis, strain CPB3-1T belonged to the genus Sporolactobacillus and was closely related to Sporolactobacillus kofuensis DSM 11701T and Sporolactobacillus spathodeae BK117-1T (both 96.7 % similarity), Sporolactobacillus inulinus NRIC 1133T and Sporolactobacillus terrae DSM 11697T (both 96.6 % similarity), and Sporolactobacillus shoreicorticis MK21-7T, Sporolactobacillus l...

Fermentation

Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-... more Astaxanthin is a carotenoid pigment extensively used in various industries. Rhodotorula sp. CP72-2, isolated from Calotropis gigantea, showed potential astaxanthin production. In this study, strain CP72-2 was identified as a putative new species in the genus Rhodotorula based on the 26S rRNA gene sequence (98% identity). It was first used as the microbial source for producing astaxanthin. Strain CP72-2 was screened for its astaxanthin production and was identified and quantified by High-Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry (LC-MS), and UV-Vis spectrophotometer. After a screening of astaxanthin production, various carbon sources, pH, temperature, and incubation period were evaluated for their effect on the astaxanthin production of strain CP72-2. Among the several experimental factors, the most efficient conditions for astaxanthin production were glucose (50 g/L), pH 4.5, 25 °C, and three days of cultivation. The assembly genome of strain ...

Biotechnology letters, 2021

OBJECTIVES This study aimed to screen, characterize, and annotate the genome along with the compa... more OBJECTIVES This study aimed to screen, characterize, and annotate the genome along with the comparison of GABA synthesis genes presented in lactic acid bacteria (LAB). RESULTS Thirty-five LAB isolates from fermented foods were screened for GABA production using thin-layer chromatography (TLC). Fifteen isolates produced GABA ranging from 0.07 to 22.94 g/L. Based on their GTG5 profiles, phenotypic, and genotypic characteristics, isolates LSI1-1, LSI1-5, LSI2-1, LSI2-2, LSI2-3, LSI2-5, and LSM3-1-4 were identified as Lactobacillus plantarum subsp. plantarum; isolate LSM1-4 was Lactobacillus argentoratensis; isolates CAB1-2, CAB1-5, CAB1-7, and LSI1-4 were Lactobacillus pentosus; and CAB1-1, LSM3-1-1 and LSM3-2-3 were Lactobacillus fermentum. Strains LSI2-1 and CAB1-7 from pickled vegetables were selected for genome analysis. The gadA gene (1410 bp, 470aa) was encountered in GABA production of both strains and no other glutamate decarboxylase (GAD) genes were found in the genomes when c...

Two hundred succinic acid-producing bacteria were isolated from cattle dung, soil, bovine rumen, ... more Two hundred succinic acid-producing bacteria were isolated from cattle dung, soil, bovine rumen, and tree bark collected in Thailand. Eighty-four isolates exhibited a clear zone on the screening plate. The production was analyzed qualitatively by the TLC method and quantitatively by the HPLC method. The succinic acid was in the range of 0.003-0.97 g/g glucose. Only 28 isolates were selected based on morphology, cultural and phenotypic characteristics. The isolates were divided into three groups. Twenty-three isolates in Group I were identified as Enterococcus, 2 isolates in Group II were identified as Lactobacillus, and 3 isolates in Group III were identified as Clostridium. Enterococcus durans (isolate NS15-dA1) and Enterococcus hirae (isolate NS15-bA2) were selected for further study for succinic acid production. The optimum conditions were 60 g/L of glucose, 30 g/L of yeast extract (NS15-dA1), and 30 g/L of tryptone (NS15-bA2) at pH 7.0 and 37 °C. The highest amounts of succinic ...

Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria

This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric aci... more This study is focused on the yield of fermentative sugars liberated from the diluted sulfuric acid pretreatment of sweet sorghum straw followed by enzymatic saccharification of a commercial cellulase (Celluclast 1.5, Novozyme). The sweet sorghum straw was mixed with 3% dilute sulfuric acid with 10%w/v of solid loading and then, pretreated at various temperatures (120-190°C) for 10 min of pretreated times. The maximum yields of glucose and xylose from sweet sorghum straw were 0.234 g/g dry substrate and 0.208 g/g dry substrate, respectively, at the pretreatment condition: 120 oC, 3%H 2 SO 4 for 10 min. In this case, a total of 50.04% of glucan and 76.41% of xylan were converted to glucose and xylose, respectively. The saccharification conditions were 1.0-7.0% of the acid pretreated sweet sorghum straw, 15-35 FPU/g-substrate of cellulase, pH from 3 to 7 and temperature from 30 to 50 o C. A maximum saccharification yield of glucose was 0.366 g/g dry substrate at the optimal condition: ...

The Journal of General and Applied Microbiology

The DDBJ accession number for the 16S rRNA gene sequence of strain SL9-6 is LC496807. The GenBank... more The DDBJ accession number for the 16S rRNA gene sequence of strain SL9-6 is LC496807. The GenBank/EMBL/DDBJ accession number of the draft genome of strain SL9-6 is VSFI00000000. Genome analysis and optimization of γ γ γ γ γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials