Felipe Ascencio | Centro de Investigaciones Biológicas del Noroeste S.C. (original) (raw)

Papers by Felipe Ascencio

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Research Square (Research Square), Apr 20, 2023

The Almaco jack (Seriola rivoliana) is a marine sh maintained in mariculture systems and frequent... more The Almaco jack (Seriola rivoliana) is a marine sh maintained in mariculture systems and frequently infested by monogenean parasites like Neobenedenia sp. Severe infestations can lead to high mortalities and economic losses for farmers. This study evaluated the effects of temperature on the immune response on Almaco jack infested with Neobenedenia sp. We exposed infested shes at temperatures of 20°C, 24°C and 30°C for 20 days and took samples of different tissues at the beginning of the experiment, and after 3 and 20 days. The tissues considered were the skin, thymus, cephalic kidney, and spleen to evaluate the relative gene expression of different genes: Hsp70, IgM, IL-1β, IL-10, and MyD88. Our results showed an increase in IL-1β gene expression in the skin after 20 days of infestation but no signi cant effect of temperature on gene expression, despite increases in infestation rates with temperature. These results show that the parasite infestation induced a local response in the skin, but that temperature has a limited direct effect on the immune system of Almaco jack.

$Research paper thumbnail of Inducción de IgA e IgG anti-omp48 en conejos inmunizados intragástricamente con una fracción extracelular de Aeromonas veronii Induction of omp48-specific IgA and IgG in rabbits intragastrically immunized with an extracellular fraction of Aeromonas veronii$

Una proteína de membrana externa de 48 kDa (omp48) de Aeromonas veronii (A. veronii), que tiene a... more Una proteína de membrana externa de 48 kDa (omp48) de Aeromonas veronii (A. veronii), que tiene afinidad por secreciones de la mucosa (tal como mucina, lactoferrina, inmunoglobulinas o colágeno), induce una respuesta inmune estadísticamente significativa al ser administrada intragástricamente a conejos blancos de Nueva Zelanda. Estudios posteriores mostraron una correlación positiva entre las inmunoglobulinas y la respuesta de células productoras de anticuerpo (CPA). La administración intragástrica del antígeno induce una respuesta vigorosa, tanto local como sistémica, principalmente de inmunoglobulinas A y G contra la omp48 de A. veronii. Estas se evidenciaron por la presencia de (i) células productoras de anticuerpos contra omp48 en el tracto gastrointestinal, bazo y sangre, y (ii) elevados niveles de anticuerpos circulantes. La estimulación del sistema inmune de mucosa mediante antígenos bacterianos inmunogénicos, puede ser una importante alternativa para el control y prevención de enfermedades asociadas a Aeromonas.

Journal of Chromatography A, 1988

In this study, five strains of microalgae were isolated from Bahia de La Paz, Baja California Sur... more In this study, five strains of microalgae were isolated from Bahia de La Paz, Baja California Sur, Mexico and further identified as Grammatophora sp., Navicula sp., Rhabdonema sp., Schizochytrium sp., and Nitzchia sp. by standard taxonomic studies. The strains were cultured outdoor and (hatched) harvest after four days. The protein, lipid, and carbohydrate composition and the fatty acid profiles of the strains were determined by gas chromatography. To assess the effect of microalgae strains in Artemia franciscana, were decapsulated the cysts ( A. franciscana ) and then cultured at outdoor conditions at one nauplius mL -1 and the microalgal density was maintained at 70 000 cells mL -1 . The experiment was conducted in 15-L tanks for twelve days. Samples were taken at the beginning and end of the experiment to assess the number, size, and weight of Artemia larvae. Rhabdonema sp. showed a lower percentage of polyunsaturated fatty acids (PUFAs), while Grammatophora sp. had the greatest...

Journal of Microbiology and Biotechnology, 2001

A simple method for studying the lectin-like interactions between Helicobacter pylori and culture... more A simple method for studying the lectin-like interactions between Helicobacter pylori and cultured human epithelial cell lines was developed using an enzyme-linked, biotin-streptavidin bacterial-adhesion assay. The present study suggests that this method is suitable for evaluating the participation of lectin interactions in the adhesion of H. pylori to cultured HeLa S3 and Kato III cells, both fixed and glycosidase-treated cells, as well as assessing glycoconjugated binding inhibition studies. The time-course and dose-dependent kinetics of the biotin-labeled H. pylori adhesion th the formaldehyde-fixed Hela S3 and Kato III cell lines exhibited saturation. In addition, the binding of the biotin-labeled H. pylori to the formaldehyde-fixed cultured cells was partially blocked by pre-incubation with glycoconjugates and polyclonal antibodies against a heparan sulfate binding protein from H. pylori.

THE JOURNAL OF MARINE BIOTECHNOLOGY, 1995

Journal of Thermal Biology, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Journal of Food Protection, 2020

ABSTRACTColletotrichum species are the most important postharvest spoilage fungi of papaya fruit.... more ABSTRACTColletotrichum species are the most important postharvest spoilage fungi of papaya fruit. The objective of this research was to evaluate the effect of temperature and relative humidity on growth rate and time for growth to become visible of five strains of Colletotrichum gloeosporioides isolated from papaya fruit in a complex medium. As a primary model, the radial growth rates were estimated using the Baranyi and Roberts model in papaya agar. The Solver MS Excel function was used to obtain the time to visible mycelium (tv). Secondary models obtained with the Rosso et al. cardinal model of inflection were applied to describe the effect of temperature on the growth rate (μ). The Arrhenius-Davey model was used to model tv. The obtained models seem to be satisfactory for describing both μ and tv. The relative humidity had an effect on μ and tv for all tested C. gloeosporioides isolates, but no model accurately described the behavior of the fungus. External validation of models w...

Aquaculture Research, 2019

In shrimp, several physiological, metabolic and gene expression patterns related to the use of ca... more In shrimp, several physiological, metabolic and gene expression patterns related to the use of carbohydrates have been associated with hypoxia since this limiting factor modifies aerobic glycolysis to anaerobic glycolysis. The aim of this work was to analyse the effect of short-term hypoxia on oxygen consumption (OC), carbohydrate content and expression of hypoxia inducible factor 1 (HIF-1), glycolysis and Krebs' cycle genes in Litopenaeus vannamei juvenile shrimps to elucidate use of energy in shrimps during the periods of low oxygen concentration commonly found in culture ponds. At low oxygen concentration, a decrease in shrimp OC, an increase in carbohydrate content and an increase in expression of HIF-1, glycolysis and Krebs' cycle genes in shrimp gills were registered. These results might indicate that exposure of L. vannamei to short-term hypoxia implies the use of carbohydrates as metabolic fuel triggered by an increase in transcription of HIF-1, glycolysis and Krebs' cycle pathway genes, as a strategy to maintain a balance between oxidative and anaerobic metabolism and to sustain the energy supply as a compensation to low oxygen availability.

Physiological and Molecular Plant Pathology, 2018

β-glucosidases participate (EC 3.2.1.21) in the activation of glycosylated and inactive forms of ... more β-glucosidases participate (EC 3.2.1.21) in the activation of glycosylated and inactive forms of various compounds during plant response to the environmental conditions. The understanding of regulatory mechanisms for β-glucosidases in each plant species and their functionality is the purpose of various investigations. Our study reports the expression analysis of 10 β-glucosidase genes in corn seedlings after a challenge infection ith Fusarium proliferatum and Ustilago maydis compared with the gene expression analysis after mechanical damage and without damage to the seedlings under in vitro culture conditions. Likewise, the heterologous sequence comparison of the β-glucosidases from Zea mays and the characterized β-glucosidases of Oryza sativa and Arabidopsis thaliana were reported. Our results suggest a potential similitude in the functionality of this group of β-glucosidase genes in maize during the response to biotic stress and the mechanical damage that also showed possible tissue-specific regulation. Our results suggest the transcription regulation of these β-glucosidases and their possible influence in the defence mechanism against fungal infections in maize.

Fish & Shellfish Immunology, 2016

Injected phage-displayed-VP28 vaccine reduces shrimp Litopenaeus vannamei mortality by white spot... more Injected phage-displayed-VP28 vaccine reduces shrimp Litopenaeus vannamei mortality by white spot syndrome virus infection,

Zentralblatt für Bakteriologie, 1993

Untersuchung der mit Gold-markiertem Fetuin inkubierten H. pylori zeigte, dag das Hamagglutinin m... more Untersuchung der mit Gold-markiertem Fetuin inkubierten H. pylori zeigte, dag das Hamagglutinin mit lose anhangendem Material an der Bakterienoberflache assoziiert war und dag das gereinigte Hamagglutinin keine Fimbrienstruktur aufwies. Die Fahigkeit zur Bindung an Sialoglycokonjugate der Erythrozytenmembran weist darauf hin, dag das Hammaglutinin einen wichtigen Besiedlungsfaktor darstellt, der H. pylori eine Bindung an ahnliche Saccharidstrukturen auf Epithelzellen ermoglicht.

uanl.mx

El uso de probióticos para el cultivo de peces marinos y dulceacuícolas, se incrementa cada vez m... more El uso de probióticos para el cultivo de peces marinos y dulceacuícolas, se incrementa cada vez más, debido a los efectos positivos que éstos confieren al hospedero. Las levaduras son probióticos, que han mostrado promover el crecimiento, la supervivencia, la actividad y la expresión de genes codificantes para las principales enzimas digestivas, cuando son administrados durante la etapa larvaria. Entre los beneficios aportados por estos microorganismos, encontramos el suministro de poliaminas, las cuales promueven diversos procesos fisiológicos vitales para el hospedero. En peces teleósteos, se ha observado que la administración de levaduras vivas en la dieta, estimula el sistema inmune y antioxidante así como la expresión de genes relacionados en el sistema inmune, confiriendo protección contra patógenos. Así mismo, en los juveniles, también se ha demostrado que se promueve la actividad de ciertas enzimas digestivas, permitiendo obtener mejores rendimientos en el crecimiento.

Journal of Applied Microbiology, 2020

Aims: To evaluate tomato epiphyte lactic acid bacteria (LAB) hydrophobicity and auto-aggregation ... more Aims: To evaluate tomato epiphyte lactic acid bacteria (LAB) hydrophobicity and auto-aggregation as an indicator of bacteria adhesion to tomato. Likewise use LAB adhesion and co-aggregation as mechanisms to antagonize pathogen attachment. Methods and Results: Fifty-four LAB were screened to evaluate their hydrophobic, auto-and coaggregative properties against Salmonella Typhimurium, Saintpaul, Montevideo and Escherichia coli O157:H7. Subsequently, tomato adhesion of Enterococcus faecium Col1-1C, Weisella cibaria 11-E-2, and W. confusa Col 1-13 with high, medium and low hydrophobicity and high co-aggregation was investigated as well as their pathogen antagonism. Results indicate that bacteria hydrophobicity and auto-aggregation correspond with LAB adhesion to tomato. Ent. faecium Col1-1C interfered in most of the pathogen adhesion and micrographs reveled that such effect could be related to the inhibition of structures-type biofilm on E. coli O157:H7 and the aggregate formation on Salmonella. Conclusions: LAB hydrophobicity and auto-aggregation can estimate bacteria adhesion to tomato and adhesive and co-aggregative properties could serve as a tool to antagonize foodborne pathogens under specific conditions. Significance and Impact of Study: This study evidence the interference of Ent. faecium Col1-1C in E. coli O157:H7 biofilm formation and Salmonella colonization.

Submission article platform - Latin American Journal of Aquatic Research, Feb 28, 2017

Developmental and Comparative Immunology, 2018

Characterization of nuclear factor of activated T-cells-c3 (NFATc3) and gene expression of upstre... more Characterization of nuclear factor of activated T-cells-c3 (NFATc3) and gene expression of upstream-downstream signaling molecules in response to immunostimulants in Pacific red snapper cells, Developmental and Comparative Immunology (2017),

Latin American Journal of Aquatic Research, Feb 28, 2017

The protective effects of two probiotic mixtures was studied using the fingerprints of the bacter... more The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5×10 5 CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.

Journal of Helminthology, Dec 18, 2017

The toxicity of water-ethanol extracts of garlic (Allium sativum), ginger (Zingiber officinale), ... more The toxicity of water-ethanol extracts of garlic (Allium sativum), ginger (Zingiber officinale), basil (Ocimum basilicum), bitter chaparro (Castela tortuousa), onion (Allium cepa) and papaya (Carica papaya) against adults, eggs and oncomiracidia of Neobenedenia spp. parasites was examined. Parasites were exposed to continuous immersion and treated as follows: extracts were tested at three dilutions: 1:10, 1:50 and 1:100 made with filtered seawater (35 g l-1); ethanol (70%) was evaluated at the same dilutions of 1:10 (7% ethanol), 1:50 (1.4% ethanol) and 1:100 (0.07% ethanol) and a seawater (35 g l-1) control. The antiparasitic effect was measured on: (1) adult survival, egg production and time to detachment from the culture vessel; (2) egg development and cumulative egg hatching; and (3) oncomiracidia survival. All three dilutions of ginger and dilutions 1:100 and 1:50 of basil extract reduced adult survival in vitro, time to detachment from the surface of the culture vessel, egg production and oncomiracidia survival. Bitter chaparro extract reduced adult egg production and oncomiracidia survival. Hatching success was significantly reduced (P < 0.05) in basil extract (1:100) to 86.6% compared to the seawater control (100%). Dilutions 1:10 of ginger and basil exhibited the highest impact on the biological parameters of Neobenedenia sp. Our study demonstrates that water-ethanol extracts of ginger, basil and bitter chaparro are toxic against Neobenedenia sp. life stages. 58 A.G. Trasviña-Moreno et al.

Developmental and Comparative Immunology, Nov 1, 2018

Debaryomyces hansenii-derived β-glucan has shown immunostimulant effect on aquaculture species an... more Debaryomyces hansenii-derived β-glucan has shown immunostimulant effect on aquaculture species and recently on goat peripheral blood leukocytes. Moreover, the marine yeast D. hansenii CBS 8339 has demonstrated to enhance fish immune response. Nonetheless, the associated immune signaling pathways induced by β-glucan from this marine yeast have not been characterized yet. This study described the effects of β-glucan from D. hansenii CBS 8339 against challenge with Escherichia coli and activation of possible mechanisms on goat peripheral blood leukocytes. The proton nuclear magnetic resonance spectra showed that D. hansenii had β-(1,3) (1,6)-glucan. The phagocytic ability enhanced after E. coli challenge, and nitric oxide production increased before and after challenge in leukocytes stimulated with D. hansenii β-glucan. In addition, an early gene expression stimulation was found related to β-glucan recognition by TLR2 and Dectin-1 receptors, intracellular regulation by Syk, TRAF6, MyD88 and transcription factor NFκB, and effector functions of pro-inflammatory cytokine, such as IL-1β and TNF-α. Interestingly, simulation with D. hansenii-derived β-glucan increased leukocyte viability after E. coli challenge. In conclusion, β-glucan from D. hansenii CBS 8339 reduced cytotoxic effects of E. coli and modulated signaling pathways and innate immune response in goat peripheral blood leukocytes.