Saurav Sinha | Central Institute of Fisheries Education, Mumbai (original) (raw)

Papers by Saurav Sinha

The study was performed for a period of 60 days to evaluate the effect of dietary carrageenan on ... more The study was performed for a period of 60 days to evaluate the effect of dietary carrageenan on growth, haematology, biochemical and innate immunity in rohu Labeo rohita. A basal diet supplemented with iota (І)-Carrageenan at 5, 10 and 20 g kg -1 was fed to three different groups of fish for 60 days and the fishes were examined after 15, 30, 45 and 60 days of experiment. The growth (absolute growth, specific growth rate and percentage weight gain), haematological (total erythrocyte count, total leucocyte count, thrombocyte count and haemoglobin value), biochemical (total serum protein, albumin, globulin and albumin-globulin ratio) and innate immune parameters (NBT and myeloperoxidase activity) were monitored at fortnight interval to assess the effect of I-Carrageenan based diet in L. rohita. All the studied growth, haematological, biochemical and innate immune parameters increased significantly (P<0.05) in carrageenan-fed groups in comparison to control. However, the group which was fed diet containing 10 g kg −1 I-Carrageenan showed the highest value of growth, haematological, biochemical and innate immune parameters on 60 th day in comparison to other groups. The study suggests that I-carrageenan at 10 g kg -1 diet enhances the innate immunity and also the overall health status in L. rohita.

Modulation of the immune responses using active bio-ingredients as a possible prophylaxis measure... more Modulation of the immune responses using active bio-ingredients as a possible prophylaxis measure has been novel prospect for aquaculture. The present study evaluated the effects of azadirachtin EC 25% on non-specific immune responses in goldfish Carassius auratus and resistance against pathogenic bacteria Aeromonas hydrophila. The experimental trial for effects of azadirachtin on immuno-haematoloical parameters in goldfish was conducted by feeding the various levels of azadirachtin as control T 0 (without azadirachtin), T 1 (0.1%), T 2 (0.2%), T 3 (0.4%), T 4 (0.8%) and T 5 (1.6%) for a period of 28 days. Fishes were challenged with A. hydrophila 28 days post feeding and relative percentage survival (%) was recorded over 14 days post infection. Immuno-haematoloical (total erythrocyte count, total leukocyte count, haemoglobin, packed cell volume, NBT activity, phagocytic activity, serum lysozyme activity, myeloperoxidase activity, total immunoglobulin) and serum biochemical parameters (serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and blood glucose) of fishes were examined at 14 and 28 days of feedings. Fish fed with azadirachtin, showed significantly (p < 0.05) enhanced TEC, TLC, Total Ig, total protein, NBT activity, serum lysozyme activity and myeloperoxidase level in different treatment groups in comparison with control group. Similarly, SGOT, SGPT and blood glucose level were found to be significantly (p < 0.05) high but PCV and Hb did not differ significantly (p > 0.05) in the treatment groups compared to control groups. Azadirachtin at the concentration of 4 g kg À1 showed significantly (p < 0.05) higher relative percentage survival (42.60%) when compared with the control against A. hydrophila infection. This study indicated that azadirachtin EC 25% (4 g kg À1 ) showed higher NBT activity, serum lysozyme, protein profiles, leukocyte counts and resistance against A. hydrophila infection and thus, can be used as a potential immunostimulant in aquaculture.

Parasitology …, 2012

Argulus are common aquatic ectoparasites that create one of the major threats to aquaculture due ... more Argulus are common aquatic ectoparasites that create one of the major threats to aquaculture due to absence of suitable therapy. Piperine, a bioactive component of Piper longum, has medicinal properties and acts as anti-inflammatory, antibacterial, and antifungal, considering eco-friendliness and costeffectiveness. The present study aimed to evaluate antiparasitic effect of piperine against an ectoparasite Argulus spp. on Carassius auratus. Artificial Argulus infection was carried out by cohabitation method, and the fishes were selected for in vivo study when intensity of Argulus infestation was observed to be 15-20 Argulus per fish. In vitro and in vivo studies were performed at different concentration 1.0 (T 1 ), 3.0 (T 2 ), 5.0 (T 3 ), 7.0 (T 4 ), and 9.0 mg l −1 (T 5 ) of piperine solution to treat Argulus for 3 and 72 h, respectively. The acute toxicity test for piperine EC 97 % against goldfish was performed for 96 h. The 96 h median lethal concentration (LC 50 ) for piperine was found to be 52.64 mg l −1 . In vitro effect of piperine solution led to 100 % mortality of Argulus at 9.0 mg l −1 in 3 h whereas, under in vivo test, the 100 % antiparasitic efficacy of piperine solution was found at 9.0 mg l −1 in 48 h. The EC 50 for 48 h was 9.0 mg l −1 , and thus, therapeutic index is 5.8. The results revealed that piperine at a concentration of 9.0 mg l −1 can be used as a potential natural agent for controlling Argulus parasite.

Parasitology …, 2012

Argulus is one of the most common and predominant ectoparasites which cause serious parasitic dis... more Argulus is one of the most common and predominant ectoparasites which cause serious parasitic disease and is a potent carrier of viruses and bacteria in the ornamental fish industry. In recent years, organic (herbs)based medicines are widely used to cure the disease, and neem (Sarbaroganibarini) medicine is very popular and effective throughout the world. The present study was conducted to find the effects of Azadirachtin against Argulus spp. on Carassius auratus under in vitro and in vivo conditions. The 96-h median lethal concentration (LC 50 ) for Azadirachtin EC 25% against Carassius auratus was found to be 82.115 mg L −1 . The antiparasitic activity test under in vitro and in vivo was evaluated at 1 (T1), 5 (T2), 10 (T3), 15 (T4) and 20 mg L −1 (T5) to treat Argulus for 3 h and 72 h, respectively. In vitro effect of Azadirachtin solution led to 100% mortality of Argulus at 20 and 15 mg L −1 for 2.5 and 3 h, respectively. Whereas, under in vivo test, the 100% antiparasitic efficacy of Azadirachtin solution was found at 15 and 20 mg L −1 for 72 and 48 h, respectively. The EC 50 for 48 h was

Abstract Argulosis hampers aquaculture production and alters the host physiology and growth. Azad... more Abstract Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups.

Parasitology …, 2012