Laiq ur rahman | Central Institute Of medicinal & Aromatic plants (original) (raw)
Papers by Laiq ur rahman
Springer eBooks, Sep 21, 2022
Indo global journal of pharmaceutical sciences, 2017
Plant Cell, Tissue and Organ Culture (PCTOC), 2021
Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicin... more Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicine for the treatment of diabetes. Phytochemical investigations of this plant showed gymnemic acids (a group of triterpenoid saponins) as the main active components. The present study aims to investigate the effectiveness of sodium nitroprusside (SNP) treatment for enhancement of cell suspension culture biomass and to evaluate their deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acid XVII contents of G. sylvestre. Callus was obtained from in vitro derived leaves of G. sylvestre on MS medium forti ed with 3.0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 2.0 mg/L Kn (Kinetin), and the same were used further to produce cell suspension cultures. Cell suspensions were exposed to different concentrations of SNP (5, 10, 20 and 40 µM) and data were collected at 20, 30 and 40 days. Out of the tested concentrations, 20 µM SNP had the highest level of cell culture growth (398.94 ± 8.32 g/L FCW and 40.00 ± 0.75 g/L DCW) on 40-day as compared to control. High-performance liquid chromatography analysis showed that maximum accumulation of deacylgymnemic acid (5.51 mg/g DCW), gymnemagenin (2.80 mg/g DCW) and gymnemic acid XVII (2.08 mg/g DCW) in 20 µM SNP treatment which is (13.43, 13.86 and17.33 folds) higher than the respective control at 40 days exposure. This research suggests that G. sylvestre cell suspension culture with optimal SNP elicitation treatment could be used as a good strategy for the large-scale production of these secondary metabolites at the industrial level.
Plant Cell, Tissue and Organ Culture (PCTOC), 2021
An incorrect concentration of Kn (Kinetin) was specified in the initial online publication. The o... more An incorrect concentration of Kn (Kinetin) was specified in the initial online publication. The original article has been corrected. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Natural Product Communications, 2009
The hydrodistilled essential oil of aerial parts of Thymus serpyllum L. (Lamiaceae), cultivated i... more The hydrodistilled essential oil of aerial parts of Thymus serpyllum L. (Lamiaceae), cultivated in the Kumaon region of western Himalaya was analyzed by GC and GCMS. Twenty-nine compounds, representing 91.8% of the oil, were identified. The major components were thymol (58.8%), p-cymene (5.7%), thymol methyl ether (4.0%), borneol (3.8%), sabinene (3.4%), γ-terpinene (3.4%) and carvacrol methyl ether (3.2%).
Scientific reports, Jan 17, 2018
Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the... more Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the form of an anesthetic and antispasmodic drug. The present study demonstrates enhanced scopolamine production from transgenic hairy root clones of Duboisia leichhardtii wherein the expression of quinolinate phosphoribosyl transferase (QPT) gene was silenced using the QPT-RNAi construct under the control of CaMV 35 S promoter. The RNAi hairy roots clones viz. P4, P7, P8, and P12 showed the enhanced synthesis of scopolamine with significant inhibition of nicotine biosynthesis. Optimization of culture duration in combination with methyl jasmonate elicitor in different concentrations (50 µM-200 µM) was carried out. Maximum synthesis of scopolamine had obtained from HR clones P7 (8.84 ± 0.117 mg/gm) on the 30 day of cultivation. Conspicuously, elicitation with wound-associated hormone methyl jasmonate enhanced the yield of scopolamine 2.2 fold (19.344 ± 0.275 mg/gm) compared to the culture la...
Frontiers in plant science, 2018
In traditional, herbal medicine, and aromatherapy, use of essential oils and their aroma compound... more In traditional, herbal medicine, and aromatherapy, use of essential oils and their aroma compounds have been known since long, for the management of various human diseases. The essential oil is a mixture of highly complex, naturally occurring volatile aroma compounds synthesized by medicinal and aromatic plants as secondary metabolites. Essential oils widely used in pharmaceutical, cosmetic, sanitary, food industry and agriculture for their antibacterial, antiviral, antifungal, antiparasitic, insecticidal, anticancer, neuroprotective, psychophysiological, and anti-aging activities. Moreover, volatile aroma compounds comprise a chemically diverse class of low molecular weight organic compounds with significant vapor pressure. However, aroma compounds produced by plants, mainly attract pollinators, seed dispersers and provide defense against pests or pathogens. However, in humans, about 300 active olfactory receptor genes are involved to detect thousands of different aroma compounds a...
African Journal of Biotechnology, 2016
Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous co... more Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous compounds possessing strong biocidal activity, thus making Tagetes plants very useful as natural source of agents for controlling pathogens such as nematodes, insects, fungi and bacteria. Hairy root cultures of Tagetes erecta L. were developed using Agrobacterium rhizogenes mediated transformation. The hairy root clones showed great variations in growth pattern and total thiophene content (0.31 to 0.96 mg/gfw). Four types of thiophenes that accumulated in root cultures of Tagetes were butenenylbithiophene (BBT), hydroxybutenenylbithiophene (BBTOH), acetoxybutenenylbithiophene (BBTOAc) and α-terthienyl (α-T). Total thiophene contents in these isolated rhizoclones were found to be four fold higher than that of wild type hairy root cultures. The developed method of producing hairy cultures of T. erecta can be used for producing thiophenes at large scale.
An experiment was conducted to determine the impact of harvesting stage and postharvest storage o... more An experiment was conducted to determine the impact of harvesting stage and postharvest storage on essential oil (EO) content and composition of clary sage (Salvia sclarea L.) cv. 'CIM-Chandni' in the Kumaon region of western Himalaya. Clary sage inflorescences were collected at five different stages viz., prior to seed setting, all seeds whitish green, half seeds brown, all seeds brown and seed shattering stages. The EO was isolated by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The EO content varied from 0.18 to 0.31% during these stages. The major components of these oils were linalool (25.38-34.32%), linalyl acetate (27.98-48.59%), D-terpineol (4.03-5.25%) and sclareol (1.02-1.59%). Storage of clary sage inflorescences under shade for 96 h prior to distillation significantly reduced the EO recovery (from 0.30 to 0.13%). Linalool increase after storage (28.86 to 37.47%) but linalyl acetate initially increased (from 39.68 to 43.30%) thereafter it decreased after storage.
Plant Signaling & Behavior, 2015
Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzym... more Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol-glucosides (SGs) in many organisms. WsSGTL1 is a SGT of Withania somnifera that has been found associated with plasma membranes. However its biological function in W.somnifera is largely unknown. In the present study, we have demonstrated through RNAi silencing of WsSGTL1 gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic W.somnifera. For this, RNAi construct (pFGC1008-WsSGTL1) was made and genetic transformation was done by Agrobacterium tumefaciens. HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of W.somnifera) and a large increase of withanolides (majorly withaferin A) content. Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of WsSGTL1 gene in W.somnifera.
Plant signaling & behavior, Jan 4, 2015
Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers ... more Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers a potential production system for iridoid glycosides. In present study we have investigated the effects of various nutrient medium formulations viz B5, MS, WP and NN, and sucrose concentrations (1-8%) on the biomass and glycoside production of selected clone (14-P) of P. kurroa hairy root. Full strength B5 medium was found to be most suitable for maximum biomass yield on the 40th day of culture (GI = 32.72 ± 0.44) followed by the NN medium of the same strength (GI = 22.9 ± 0.43). Secondary metabolite production was 1.1 and 1.3 times higher in half strength B5 medium respectively in comparison to MS medium. Maximum biomass accumulation along with the maximum picroliv content was achieved with 4% sucrose concentration in basal medium. RT vitamin and Thiamine-HCl effected the growth and secondary metabolite production of hairy roots growing on MS medium but did not show any effect on other ...
Journal of environmental biology / Academy of Environmental Biology, India, 2014
Farmers in hilly regions experience low production potential and resource use efficiency due to l... more Farmers in hilly regions experience low production potential and resource use efficiency due to low valued crops and poorsoil health. Geranium (Pelargonium graveolens L.) is a vegetatively propagated initially slow growing, high value aromatic crop. Potato (Solanum tuberosum L.) is also vegetatively propagated high demand cash crop. A field experiment was carried out in temperate climate to investigate the influence of geranium intercropping at different row strips (1:1 and 1:2) and plant density (60 x 45, 75 x 45 and 90 x 45 cm) with potato intercrop on biomass, oil yield, monetary advantage and soil quality parameters. The row spacing 60x45cm and row strip 1:1 was found to be superior and produced 92 t ha(-1) and 14 kg ha(-1) biomass and oil yield, respectively. The row strip 1:2 intercrop earned a maximum 2107,followedby2107, followed by 2107,followedby1862 with row strip 1:1 at 60 x 45 cm plant density. Significant variations were noticed in soil organic carbon (Corg), total N (Nt), available nutrients, so...
International Journal of Recycling of Organic Waste in Agriculture, 2014
Background Intercropping systems significantly influence chemical and biological properties of th... more Background Intercropping systems significantly influence chemical and biological properties of the soil. Our objective was to evaluate the effects of intercropping systems on soil organic carbon (SOC), total Kjeldahl nitrogen (TKN), available N, P and K, soil microbial biomass carbon (MB C) and microbial biomass nitrogen (MB N) under geranium (Pelargonium graveolens L.)-based intercropping systems. Results Geranium-based intercropping (with cereals, pulses, fodder, and vegetables) resulted in increase of SOC and TKN by 7.8-69.2 % and 10.7-92.8 %, respectively, over geranium alone. Similarly, microbial respiration was higher under geranium intercropped with oat (36.3 %) followed by wheat (30.5 %) and barley (12.5 %) as compared to that under geranium alone. Soil MB C accounted for 3.3-4.7 % of SOC content and soil MB N accounted for 3.1-3.5 % of TKN under different intercropping conditions. A higher CO 2 evolution rate and a wider soil MB C / MB N ratio were recorded with cereals and fodders. Conclusions It is concluded that the build-up of the organic matter and enhancement of soil MB C in the intercropping study should promote long-term stability of soil health.
Journal of the Serbian Chemical Society, 2010
The essential oil content in th e inflorescence of lavender (Lavandula angustifolia Mill.) cultiv... more The essential oil content in th e inflorescence of lavender (Lavandula angustifolia Mill.) cultivated in the mid hills of Uttarakhand was found to be 2.8 % based o n the fresh weight. The oil was an alysed by capillary GC and GC-MS. Thirty seven con stituents, representing 97.81 % of the o il were identified. The major components of the oil were linalyl acetate (47.56 %), linalool (28.06 %), lavanduly l acetate (4.34 %) and α-terpineol (3.75 %). The quality o f lavender oil pro duced in India was found to b e comparable to that produced in Hungary,
Journal of the Chilean Chemical Society, 2012
To investigate the seasonal influence on essential oil content and composition of rosemary (Rosma... more To investigate the seasonal influence on essential oil content and composition of rosemary (Rosmarinus officinalis) cultivar 'CIM-Hariyali' a study was conducted in Kumaon region of western Himalaya. Essential oil content was found to vary from 1.0% to 1.14% during the year. GC and GC-MS analyses enabled to identify thirty components representing 95.33%-97.03% of the total oil composition. Main components of the oils were 1,8-cineole (22.61%-23.85%), camphor (24.40%-25.85%), α-pinene (10.74%-12.59%), verbenone (4.90%-5.77%), camphene (5.46%-6.16%), β-pinene (3.28%-4.02%), limonene (2.86%-3.39%) and β-myrcene (1.89%-1.95%). The study clearly showed that there were no drastic changes in the essential oil content and composition of rosemary due to season. Therefore, the crop may be harvested in any season to get good quality oil in hilly region of north India.
Industrial Crops and Products, 2008
Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium grave... more Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium graveolens L'Her Ex Ait. cv. Hemanti were successfully transferred to the field in Northern India for evaluation. Two distinct morphotypes were described on the basis of leaf dentation-one resembling the parental cultivar in having highly dentated leaves (HDL) and the other with less dentated leaves (LDL). After repeated field-testing for 3 consecutive years, the HDL clones closely resembled the parental cultivar with respect to the different quantity and quality determining traits, while the LDL group was clearly different. The field established LDL somaclones possessed higher herb yield, number of branches and other oil yield attributing traits as compared to the HDL clones and the parent cultivar. The chemical investigations of the essential oil revealed significant differences between the LDL clones, the HDL clones and the control. Selection of such somaclones, which are superior to the parental in most of the quantitative and qualitative traits and show better adaptability to different areas of cultivation, will help towards commercialization of geranium in India.
Crop Protection, 2008
A protocol has been established for the induction of disease resistance in callus cultures of ros... more A protocol has been established for the induction of disease resistance in callus cultures of rose-scented geranium, Pelargonium graveolens cv. Hemanti against leaf blight disease caused by the fungal pathogen Alternaria alternata. The callus cultures were raised and maintained on semi-solid Murashige and Skoog's (MS) medium supplemented with 10 mg l À1 Kn and 1.0 mg l À1 NAA. The calli were subjected to various concentrations of culture filtrate (0%, 4%, 8%, 12%, 16% and 20%) obtained from A. alternata. Resistant calli were selected and placed on regeneration medium (MS supplemented with 0.5 mg l À1 BAP and 0.1 mg l À1 NAA). The regenerants were confirmed for A. alternata resistance by exposing their leaves to the same concentrations of culture filtrate as used previously. While the parental wild type demonstrated typical susceptibility, the leaves of putative resistant clones remained green and viable in the presence of toxin and regenerated shoots directly on the toxin-free regeneration medium (MS supplemented with 5 mg l À1 Kn and l mg l À1 NAA). The above experiment demonstrated the induction of disease resistance in rose-scented geranium plants at the cellular level. This approach could be successfully exploited in raising new disease-resistant cultivars in geranium against various fungal pathogens.
… Access Journal of …, 2010
Mints comprise a group of species of the genus Mentha belonging to the family Lamiaceae. The aeri... more Mints comprise a group of species of the genus Mentha belonging to the family Lamiaceae. The aerial parts of the herb on distillation yields essential oil containing a large number of aroma chemicals like menthol, menthone, isomenthone, menthofuran, carvone, linalool, linalyl acetate and piperitenone oxide which are used in pharmaceutical, food, flavour, cosmetics, beverages and allied industries. Mint oils are mainly produced in Argentina,
Ocimum sanctum is a multipurpose herb with highly significant medicinal properties. An in vitro d... more Ocimum sanctum is a multipurpose herb with highly significant medicinal properties. An in vitro direct regeneration protocol for propagation of a valuable medicinal plant Ocimum sanctum, using petiole explants has been successfully developed. The protocol employed regeneration of shoots directly, without any intervening callus using Murashige and Skoog (MS) medium fortified with 3 mg L-1 BAP + 1 mg L-1 NAA. The maximum regeneration frequency of 98% with 9.6 shoots per explants was achieved. Agrobacterium tumefaciens mediated genetic transformation (ATMT) protocol (transient and stable) was established using LBA4404 strain harboring pBI121 with uid-A reporter gene and neomycin phosphotransferase (npt-II) as selection marker. The putative transformants were screened on MS with 50 mg L-1 kanamycin and subsequently rooted on the half-strength MS medium. The confirmation was done via polymerase chain reaction (PCR) using npt-II and gus-A gene-specific primers. The maximum stable transfor...
Plant cell, tissue and …, 2007
Springer eBooks, Sep 21, 2022
Indo global journal of pharmaceutical sciences, 2017
Plant Cell, Tissue and Organ Culture (PCTOC), 2021
Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicin... more Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an e cient Ayurvedic traditional medicine for the treatment of diabetes. Phytochemical investigations of this plant showed gymnemic acids (a group of triterpenoid saponins) as the main active components. The present study aims to investigate the effectiveness of sodium nitroprusside (SNP) treatment for enhancement of cell suspension culture biomass and to evaluate their deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acid XVII contents of G. sylvestre. Callus was obtained from in vitro derived leaves of G. sylvestre on MS medium forti ed with 3.0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 2.0 mg/L Kn (Kinetin), and the same were used further to produce cell suspension cultures. Cell suspensions were exposed to different concentrations of SNP (5, 10, 20 and 40 µM) and data were collected at 20, 30 and 40 days. Out of the tested concentrations, 20 µM SNP had the highest level of cell culture growth (398.94 ± 8.32 g/L FCW and 40.00 ± 0.75 g/L DCW) on 40-day as compared to control. High-performance liquid chromatography analysis showed that maximum accumulation of deacylgymnemic acid (5.51 mg/g DCW), gymnemagenin (2.80 mg/g DCW) and gymnemic acid XVII (2.08 mg/g DCW) in 20 µM SNP treatment which is (13.43, 13.86 and17.33 folds) higher than the respective control at 40 days exposure. This research suggests that G. sylvestre cell suspension culture with optimal SNP elicitation treatment could be used as a good strategy for the large-scale production of these secondary metabolites at the industrial level.
Plant Cell, Tissue and Organ Culture (PCTOC), 2021
An incorrect concentration of Kn (Kinetin) was specified in the initial online publication. The o... more An incorrect concentration of Kn (Kinetin) was specified in the initial online publication. The original article has been corrected. Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Natural Product Communications, 2009
The hydrodistilled essential oil of aerial parts of Thymus serpyllum L. (Lamiaceae), cultivated i... more The hydrodistilled essential oil of aerial parts of Thymus serpyllum L. (Lamiaceae), cultivated in the Kumaon region of western Himalaya was analyzed by GC and GCMS. Twenty-nine compounds, representing 91.8% of the oil, were identified. The major components were thymol (58.8%), p-cymene (5.7%), thymol methyl ether (4.0%), borneol (3.8%), sabinene (3.4%), γ-terpinene (3.4%) and carvacrol methyl ether (3.2%).
Scientific reports, Jan 17, 2018
Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the... more Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the form of an anesthetic and antispasmodic drug. The present study demonstrates enhanced scopolamine production from transgenic hairy root clones of Duboisia leichhardtii wherein the expression of quinolinate phosphoribosyl transferase (QPT) gene was silenced using the QPT-RNAi construct under the control of CaMV 35 S promoter. The RNAi hairy roots clones viz. P4, P7, P8, and P12 showed the enhanced synthesis of scopolamine with significant inhibition of nicotine biosynthesis. Optimization of culture duration in combination with methyl jasmonate elicitor in different concentrations (50 µM-200 µM) was carried out. Maximum synthesis of scopolamine had obtained from HR clones P7 (8.84 ± 0.117 mg/gm) on the 30 day of cultivation. Conspicuously, elicitation with wound-associated hormone methyl jasmonate enhanced the yield of scopolamine 2.2 fold (19.344 ± 0.275 mg/gm) compared to the culture la...
Frontiers in plant science, 2018
In traditional, herbal medicine, and aromatherapy, use of essential oils and their aroma compound... more In traditional, herbal medicine, and aromatherapy, use of essential oils and their aroma compounds have been known since long, for the management of various human diseases. The essential oil is a mixture of highly complex, naturally occurring volatile aroma compounds synthesized by medicinal and aromatic plants as secondary metabolites. Essential oils widely used in pharmaceutical, cosmetic, sanitary, food industry and agriculture for their antibacterial, antiviral, antifungal, antiparasitic, insecticidal, anticancer, neuroprotective, psychophysiological, and anti-aging activities. Moreover, volatile aroma compounds comprise a chemically diverse class of low molecular weight organic compounds with significant vapor pressure. However, aroma compounds produced by plants, mainly attract pollinators, seed dispersers and provide defense against pests or pathogens. However, in humans, about 300 active olfactory receptor genes are involved to detect thousands of different aroma compounds a...
African Journal of Biotechnology, 2016
Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous co... more Marigold (Tagetes spp.) is a source of thiophenes, which are a group of heterocyclic sulfurous compounds possessing strong biocidal activity, thus making Tagetes plants very useful as natural source of agents for controlling pathogens such as nematodes, insects, fungi and bacteria. Hairy root cultures of Tagetes erecta L. were developed using Agrobacterium rhizogenes mediated transformation. The hairy root clones showed great variations in growth pattern and total thiophene content (0.31 to 0.96 mg/gfw). Four types of thiophenes that accumulated in root cultures of Tagetes were butenenylbithiophene (BBT), hydroxybutenenylbithiophene (BBTOH), acetoxybutenenylbithiophene (BBTOAc) and α-terthienyl (α-T). Total thiophene contents in these isolated rhizoclones were found to be four fold higher than that of wild type hairy root cultures. The developed method of producing hairy cultures of T. erecta can be used for producing thiophenes at large scale.
An experiment was conducted to determine the impact of harvesting stage and postharvest storage o... more An experiment was conducted to determine the impact of harvesting stage and postharvest storage on essential oil (EO) content and composition of clary sage (Salvia sclarea L.) cv. 'CIM-Chandni' in the Kumaon region of western Himalaya. Clary sage inflorescences were collected at five different stages viz., prior to seed setting, all seeds whitish green, half seeds brown, all seeds brown and seed shattering stages. The EO was isolated by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The EO content varied from 0.18 to 0.31% during these stages. The major components of these oils were linalool (25.38-34.32%), linalyl acetate (27.98-48.59%), D-terpineol (4.03-5.25%) and sclareol (1.02-1.59%). Storage of clary sage inflorescences under shade for 96 h prior to distillation significantly reduced the EO recovery (from 0.30 to 0.13%). Linalool increase after storage (28.86 to 37.47%) but linalyl acetate initially increased (from 39.68 to 43.30%) thereafter it decreased after storage.
Plant Signaling & Behavior, 2015
Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzym... more Sterol glycosyltransferases (SGTs) belong to family 1 of glycosyltransferases (GTs) and are enzymes responsible for synthesis of sterol-glucosides (SGs) in many organisms. WsSGTL1 is a SGT of Withania somnifera that has been found associated with plasma membranes. However its biological function in W.somnifera is largely unknown. In the present study, we have demonstrated through RNAi silencing of WsSGTL1 gene that it performs glycosylation of withanolides and sterols resulting in glycowithanolides and glycosylated sterols respectively, and affects the growth and development of transgenic W.somnifera. For this, RNAi construct (pFGC1008-WsSGTL1) was made and genetic transformation was done by Agrobacterium tumefaciens. HPLC analysis depicts the reduction of withanoside V (the glycowithanolide of W.somnifera) and a large increase of withanolides (majorly withaferin A) content. Also, a significant decrease in level of glycosylated sterols has been observed. Hence, the obtained data provides an insight into the biological function of WsSGTL1 gene in W.somnifera.
Plant signaling & behavior, Jan 4, 2015
Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers ... more Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers a potential production system for iridoid glycosides. In present study we have investigated the effects of various nutrient medium formulations viz B5, MS, WP and NN, and sucrose concentrations (1-8%) on the biomass and glycoside production of selected clone (14-P) of P. kurroa hairy root. Full strength B5 medium was found to be most suitable for maximum biomass yield on the 40th day of culture (GI = 32.72 ± 0.44) followed by the NN medium of the same strength (GI = 22.9 ± 0.43). Secondary metabolite production was 1.1 and 1.3 times higher in half strength B5 medium respectively in comparison to MS medium. Maximum biomass accumulation along with the maximum picroliv content was achieved with 4% sucrose concentration in basal medium. RT vitamin and Thiamine-HCl effected the growth and secondary metabolite production of hairy roots growing on MS medium but did not show any effect on other ...
Journal of environmental biology / Academy of Environmental Biology, India, 2014
Farmers in hilly regions experience low production potential and resource use efficiency due to l... more Farmers in hilly regions experience low production potential and resource use efficiency due to low valued crops and poorsoil health. Geranium (Pelargonium graveolens L.) is a vegetatively propagated initially slow growing, high value aromatic crop. Potato (Solanum tuberosum L.) is also vegetatively propagated high demand cash crop. A field experiment was carried out in temperate climate to investigate the influence of geranium intercropping at different row strips (1:1 and 1:2) and plant density (60 x 45, 75 x 45 and 90 x 45 cm) with potato intercrop on biomass, oil yield, monetary advantage and soil quality parameters. The row spacing 60x45cm and row strip 1:1 was found to be superior and produced 92 t ha(-1) and 14 kg ha(-1) biomass and oil yield, respectively. The row strip 1:2 intercrop earned a maximum 2107,followedby2107, followed by 2107,followedby1862 with row strip 1:1 at 60 x 45 cm plant density. Significant variations were noticed in soil organic carbon (Corg), total N (Nt), available nutrients, so...
International Journal of Recycling of Organic Waste in Agriculture, 2014
Background Intercropping systems significantly influence chemical and biological properties of th... more Background Intercropping systems significantly influence chemical and biological properties of the soil. Our objective was to evaluate the effects of intercropping systems on soil organic carbon (SOC), total Kjeldahl nitrogen (TKN), available N, P and K, soil microbial biomass carbon (MB C) and microbial biomass nitrogen (MB N) under geranium (Pelargonium graveolens L.)-based intercropping systems. Results Geranium-based intercropping (with cereals, pulses, fodder, and vegetables) resulted in increase of SOC and TKN by 7.8-69.2 % and 10.7-92.8 %, respectively, over geranium alone. Similarly, microbial respiration was higher under geranium intercropped with oat (36.3 %) followed by wheat (30.5 %) and barley (12.5 %) as compared to that under geranium alone. Soil MB C accounted for 3.3-4.7 % of SOC content and soil MB N accounted for 3.1-3.5 % of TKN under different intercropping conditions. A higher CO 2 evolution rate and a wider soil MB C / MB N ratio were recorded with cereals and fodders. Conclusions It is concluded that the build-up of the organic matter and enhancement of soil MB C in the intercropping study should promote long-term stability of soil health.
Journal of the Serbian Chemical Society, 2010
The essential oil content in th e inflorescence of lavender (Lavandula angustifolia Mill.) cultiv... more The essential oil content in th e inflorescence of lavender (Lavandula angustifolia Mill.) cultivated in the mid hills of Uttarakhand was found to be 2.8 % based o n the fresh weight. The oil was an alysed by capillary GC and GC-MS. Thirty seven con stituents, representing 97.81 % of the o il were identified. The major components of the oil were linalyl acetate (47.56 %), linalool (28.06 %), lavanduly l acetate (4.34 %) and α-terpineol (3.75 %). The quality o f lavender oil pro duced in India was found to b e comparable to that produced in Hungary,
Journal of the Chilean Chemical Society, 2012
To investigate the seasonal influence on essential oil content and composition of rosemary (Rosma... more To investigate the seasonal influence on essential oil content and composition of rosemary (Rosmarinus officinalis) cultivar 'CIM-Hariyali' a study was conducted in Kumaon region of western Himalaya. Essential oil content was found to vary from 1.0% to 1.14% during the year. GC and GC-MS analyses enabled to identify thirty components representing 95.33%-97.03% of the total oil composition. Main components of the oils were 1,8-cineole (22.61%-23.85%), camphor (24.40%-25.85%), α-pinene (10.74%-12.59%), verbenone (4.90%-5.77%), camphene (5.46%-6.16%), β-pinene (3.28%-4.02%), limonene (2.86%-3.39%) and β-myrcene (1.89%-1.95%). The study clearly showed that there were no drastic changes in the essential oil content and composition of rosemary due to season. Therefore, the crop may be harvested in any season to get good quality oil in hilly region of north India.
Industrial Crops and Products, 2008
Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium grave... more Several randomly selected glasshouse grown somaclones of rose scented geranium, Pelargonium graveolens L'Her Ex Ait. cv. Hemanti were successfully transferred to the field in Northern India for evaluation. Two distinct morphotypes were described on the basis of leaf dentation-one resembling the parental cultivar in having highly dentated leaves (HDL) and the other with less dentated leaves (LDL). After repeated field-testing for 3 consecutive years, the HDL clones closely resembled the parental cultivar with respect to the different quantity and quality determining traits, while the LDL group was clearly different. The field established LDL somaclones possessed higher herb yield, number of branches and other oil yield attributing traits as compared to the HDL clones and the parent cultivar. The chemical investigations of the essential oil revealed significant differences between the LDL clones, the HDL clones and the control. Selection of such somaclones, which are superior to the parental in most of the quantitative and qualitative traits and show better adaptability to different areas of cultivation, will help towards commercialization of geranium in India.
Crop Protection, 2008
A protocol has been established for the induction of disease resistance in callus cultures of ros... more A protocol has been established for the induction of disease resistance in callus cultures of rose-scented geranium, Pelargonium graveolens cv. Hemanti against leaf blight disease caused by the fungal pathogen Alternaria alternata. The callus cultures were raised and maintained on semi-solid Murashige and Skoog's (MS) medium supplemented with 10 mg l À1 Kn and 1.0 mg l À1 NAA. The calli were subjected to various concentrations of culture filtrate (0%, 4%, 8%, 12%, 16% and 20%) obtained from A. alternata. Resistant calli were selected and placed on regeneration medium (MS supplemented with 0.5 mg l À1 BAP and 0.1 mg l À1 NAA). The regenerants were confirmed for A. alternata resistance by exposing their leaves to the same concentrations of culture filtrate as used previously. While the parental wild type demonstrated typical susceptibility, the leaves of putative resistant clones remained green and viable in the presence of toxin and regenerated shoots directly on the toxin-free regeneration medium (MS supplemented with 5 mg l À1 Kn and l mg l À1 NAA). The above experiment demonstrated the induction of disease resistance in rose-scented geranium plants at the cellular level. This approach could be successfully exploited in raising new disease-resistant cultivars in geranium against various fungal pathogens.
… Access Journal of …, 2010
Mints comprise a group of species of the genus Mentha belonging to the family Lamiaceae. The aeri... more Mints comprise a group of species of the genus Mentha belonging to the family Lamiaceae. The aerial parts of the herb on distillation yields essential oil containing a large number of aroma chemicals like menthol, menthone, isomenthone, menthofuran, carvone, linalool, linalyl acetate and piperitenone oxide which are used in pharmaceutical, food, flavour, cosmetics, beverages and allied industries. Mint oils are mainly produced in Argentina,
Ocimum sanctum is a multipurpose herb with highly significant medicinal properties. An in vitro d... more Ocimum sanctum is a multipurpose herb with highly significant medicinal properties. An in vitro direct regeneration protocol for propagation of a valuable medicinal plant Ocimum sanctum, using petiole explants has been successfully developed. The protocol employed regeneration of shoots directly, without any intervening callus using Murashige and Skoog (MS) medium fortified with 3 mg L-1 BAP + 1 mg L-1 NAA. The maximum regeneration frequency of 98% with 9.6 shoots per explants was achieved. Agrobacterium tumefaciens mediated genetic transformation (ATMT) protocol (transient and stable) was established using LBA4404 strain harboring pBI121 with uid-A reporter gene and neomycin phosphotransferase (npt-II) as selection marker. The putative transformants were screened on MS with 50 mg L-1 kanamycin and subsequently rooted on the half-strength MS medium. The confirmation was done via polymerase chain reaction (PCR) using npt-II and gus-A gene-specific primers. The maximum stable transfor...
Plant cell, tissue and …, 2007