Daniel Robledo | Centro de Investigacion y Estudios Avanzados del IPN (original) (raw)
Books by Daniel Robledo
Fucoidans are sulfated polysaccharides with proven pharmacological effects localized in the cell ... more Fucoidans are sulfated polysaccharides with proven pharmacological effects localized in the cell wall of marine brown algae. The majority of studies have been performed with temperate brown algal species, but in recent years, the evaluation of species from tropical areas has been growing. The aim of this study was to determine the protective effect of fucoidans extracted from the tropical brown seaweeds Dictyota ciliolata, Padina sanctae-crucis, and Sargassum fluitans, against oxidative stress (OS). The D. ciliolata fucoidan (FDc) exhibited the highest reactive oxygen species (ROS) scavenging activity (26%), followed by P. sanctaecrucis fucoidan (FPs) (22%) and S. fluitans fucoidan (FSf) (14%). No cytotoxic effect was detected for any of the extracted fucoidans at a concentration of 2 mg mL −1 . Not only did the fucoidans tested show protective effect against OS by reducing ROS generation, but they also increased the glutathione (GSH) level and restored catalase (CAT) activity. Fucoidans obtained from tropical seaweeds could be used as a potential natural ingredient for functional foods.
Phenolics, sometimes called polyphenolic compounds, are present in a variety of terrestrial and m... more Phenolics, sometimes called polyphenolic compounds, are present in a variety of terrestrial and marine plants. They are utilized as important components of both human and animal diets for their antioxidant capacity. More than 8000 phenolic structures are currently known (Bravo 1998). To date, most polyphenols isolated from marine sources and referenced in the literature are of macroalgal origin (Li et al. 2011). The structures of natural polyphenols vary from simple molecules, such as phenolic
acids and other simple polyphenolic compounds, to the more complex phlorotannins, which consist of polymeric structures made up of units of phloroglucinol (1,3,5-trihydroxybenzene), typically isolated from marine brown algae or Phaeophyceae (Harborne 1980; Ragan & Glombitza 1986). Polyphenols exhibit a wide range of biological effects as a consequence of their antioxidant properties. The relatively high concentration of phenolic compounds in marine algae species contributes
to their antioxidant properties, which can be of benefit in reducing oxidative reactions deleterious to health. Oxidative stress in the human cell occurs during disease conditions or when optimal nutrition is lacking. Under these circumstances, the so-called “reactive oxygen species” (ROS) are available to initiate lipid peroxidation and damage to other biomolecules. Current evidence strongly supports the contribution of phenolic compounds to the prevention of cardiovascular diseases (CVDs) and cancers,
and suggests their role in the prevention of neurodegenerative diseases and diabetes mellitus (Urquiaga & Leighton 2000). This chapter reviews some of the chemical and biological properties of marine algae phenolics as natural antioxidants and discusses their protective effects in biological systems and their potential
activities in food products.
Cellular Origin, Life in Extreme Habitats and Astrobiology Volume 16, 2011, DOI: 10.1007/978-90-4... more Cellular Origin, Life in Extreme Habitats and Astrobiology
Volume 16, 2011, DOI: 10.1007/978-90-481-9316-5
All Flesh Is Grass
Plant-Animal Interrelationships
Zvy Dubinsky and Joseph Seckbach
Se presenta una valiosa compilación de temas relacionado con la Biodiversidad de Yucatán, sus uso... more Se presenta una valiosa compilación de temas relacionado con la Biodiversidad de Yucatán, sus usos, amenazas y potencialidades, así como la importancia que ha tenido la riqueza biológica en el desarrollo de la sociedad y la historia de los habitantes de este Estado.
Este libro está conformado de 4 grandes apartados, 10 capítulos y 171 contribuciones temáticas con la participación de 250 autores, de 55 instituciones académicas.
Esta Obra constituye la compilación de información más actualizada de los distintos grupos biológicos que ocurren en Yucatán, incluyendo plantas, animales vertebrados e invertebrados, y microorganismos, lo cual nos permite tener una idea más clara del nivel de conocimiento que se tiene de la Diversidad Biológica de Estado. Además, nos acerca a conocer la diversidad de usos para los cuales se emplean los distintos organismos biológicos y sus partes en la elaboración de bienes que proveen, alimentos, medicinas, utensilios y materiales de construcción, así como energía, elementos todos que permiten a las poblaciones locales satisfacer algunas de sus necesidades más básicas. Asimismo, se presentan diversas actividades productivas del hombre relacionadas con biodiversidad, así como sus impactos sobre los ecosistemas del Estado.
Esperamos firmemente que esta obra, sobre la riqueza biológica de Yucatán y su relación con las actividades productivas del hombre, sea un importante instrumento educativo y de planeación para ayudar al desarrollo de políticas públicas en materia ambiental en el Estado.
The importance of seaweed resources in the country its not only due to species diversity and wide... more The importance of seaweed resources in the country its not only due to species diversity and wide geographical distribution, but also due to its economic importance. Until now most of the phycological studies have focused on the elaboration of taxonomic listing with the idea of having all the marine flora from the country registred. Nevertheless, in this way the knowledge of our seaweed resources its limited without having identified abundance and seasonality of the potentially economic species along the coast of Mexico. In this study we present the evaluation of economic important species from the Yucatan coast with emphasis to its potential use for human consumption and polysaccharide extraction for the food industry.
Papers by Daniel Robledo
Journal of Experimental Botany, 2021
Journal of Experimental Botany, 2020
Short-term effects of pCO2 (700–380 ppm; High carbon (HC) and Low carbon (LC), respectively) and ... more Short-term effects of pCO2 (700–380 ppm; High carbon (HC) and Low carbon (LC), respectively) and nitrate content (50–5 µM; High nitrogen (HN) and Low nitrogen (LN), respectively on photosynthesis were investigated in Ulva rigida (Chlorophyta) under solar radiation (in-situ) and in the laboratory under artificial light (ex-situ). After six days of incubation at ambient temperature (AT), algae were subjected to a 4 °C temperature increase (AT+4 °C) for 3 d. Both in-situ and ex-situ maximal electron transport rate (ETRmax) and in situ gross photosynthesis (GP), measured by O2 evolution, presented highest values under HCHN, and lowest under HCLN, across all measuring systems. Maximal quantum yield (Fv/Fm), and ETRmax of photosystem (PS) II [ETR(II)max] and PSI [ETR(I)max], decreased under HCLN at AT+4 °C. Ex situ ETR was higher than in situ ETR. At noon, Fv/Fm decreased (indicating photoinhibition), whereas ETR(II)max and maximal non-photochemical quenching (NPQmax) increased. ETR(II)ma...
Hydrobiologia, 1991
... If the best commercial strategy for vegetative micropropagation is to maximize propagules per... more ... If the best commercial strategy for vegetative micropropagation is to maximize propagules per donor-plant ratios, the logical strategy would be to reduce ... Somatic-cell isolation in seaweeds is not a dif-ficult task as it has been achieved by partial enzymatic digestion of thalli (in ...
European Journal of Phycology, 1994
The filamentous brown algae Feldmannia simplex and F. irregular~ are attacked by aquatic viruses,... more The filamentous brown algae Feldmannia simplex and F. irregular~ are attacked by aquatic viruses, which propagate in deformed sporangia of the host plants. In order to evaluate a possible detrimental effect of the pathogen, photosynthesis-irradiance response curves and pigment concentrations of healthy and infected plants were compared. Photosynthetic performance of infected plants was significantly reduced and associated with a decrease in chlorophyll a and c content. In Feldmannia irregularis, which had a relatively low photosynthetic capacity, the virus infection produced a more severe effect than in F. simplex.
SPIE Proceedings, 2007
The green microalga Chlamydomonas reinhardtii is proposed to produce hydrogen in a low-cost syste... more The green microalga Chlamydomonas reinhardtii is proposed to produce hydrogen in a low-cost system using the solar radiation in Yucatan, Mexico. A two-step process is necessary with a closed photobioreactor, in which the algae are firstly growth and then induced for hydrogen generation. Preliminary results are presented in this work with some planning for the future. Different culture broths, temperatures and light intensities were tested for biomass and hydrogen production in laboratory conditions. The first experiments in external conditions with solar radiation and without temperature control have been performed, showing the potential of this technique at larger scales. However, some additional work must be done in order to optimize the culture maintenance, particularly in relation with the temperature control, the light radiation and the carbon dioxide supply, with the idea of keeping an economic production.
Journal of Applied Phycology, 2006
The effects of plant growth regulators on callus induction rate and regeneration of K. alvarezii ... more The effects of plant growth regulators on callus induction rate and regeneration of K. alvarezii explants was evaluated. K. alvarezii calluses were induced in vitro with kinetin (K), 6-benzylaminopurine (B), 1naphtalene acetic acid (N) and spermine (S). After 30 days, K. alvarezii explants produced filamentous calluses and isolated crystalline filaments growing from the medullar region and from cortical cells at the cut edge. The plant growth regulators 1-naphtalene acetic acid (1 mg L −1) and 6-benzylaminopurine (1 mg L −1) and the 1-naphtalene acetic acid + kinetin + spermine (1, 1, 0.018 mg L −1 respectively) combination produced 85 to 129% more calluses, with significant differences versus the control (p < 0.05). Spermine at 0.018 mg L −1 produced calluses in the apical, intercalary and basal regions of explants. Spermine also reduced callus induction time to 7 days, which is faster than previously reported induction times with other plant growth regulators. An airlift bioreactor was designed and characterized to micropropagate K. alvarezii calluses. The bioreactor had mixing times ranging from 4.6-10.3 s at T 90 and T 95 , which is shorter than those for the
Marine Drugs, 2022
Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. ... more Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC50 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC50 of 0.38 μg/mL during the...
Phycologia, 2019
Seaweed farming has now expanded across several continents from South East Asia to South America,... more Seaweed farming has now expanded across several continents from South East Asia to South America, Northern Europe, Canada and East Africa, contributing to global food security, supporting rural livelihoods, alleviating poverty and improving the health of oceans. Latin America (LA) covers a vast geographical area, which includes four different oceanic domains (Temperate Northern Pacific, Tropical Eastern Pacific, Temperate South America and Tropical Atlantic) and encompasses many types of coastal ecosystems with a wide range of seaweed species. LA has major potential for the development of seaweed farming activities; however, almost all the production is based on the harvesting of natural beds. This review describes the development of and prospects for the aquaculture seaweed industry in LA. The status of the seaweed aquaculture sector for green, brown and red seaweed and the main industry challenges are addressed. Regulation in the primary countries is also discussed. The expansion of the aquaculture industry in this region can be improved with new strains and farming methodologies, diversification of species, market expansion and an increase in domestic demand.
Natural Product Communications, 2017
Polysaccharides extracted from Rhodymenia pseudopalmata, Solieria filiformis, Hydropuntia cornea ... more Polysaccharides extracted from Rhodymenia pseudopalmata, Solieria filiformis, Hydropuntia cornea (Rhodophyta) and Sargassum fluitans (Phaeophyceae) were evaluated for its cytotoxic and antiviral activities against Herpes simplex virus (HSV-Type 1). Chemical structures were characterized by FT-IR spectroscopy and 13C-NMR analyses. Polysaccharides from Sargassum fluitans (EC50 = 42.8 μg/ml) and Solieria filiformis (EC50 = 136.0 μg/ml) showed antiviral activity against herpes simplex virus type-I in vitro at a multiplicity of infection (MOI) of 0.01 ID50/cells without cytotoxicity (1–200 μg/mL). The activity observed suggests that sulphation, molecular weight and carbohydrate nature of these polysaccharides may be involved in this activity. To better understand the antiviral activity of the polysaccharides evaluated, it seems important to study the mechanism of action involved. These polysaccharides could be studied further to evaluate their potential use as antiviral drugs.
Marine Drugs, 2018
Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefine... more Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefinery concept applied to seaweed facilitates the extraction of many chemical constituents from the same biomass ensuring that the resource is used fully, generating few residues through a succession of extraction steps. In the present study, the biomass of the carragenophyte Solieria filiformis (Rhodophyta, Gigartinales) cultured in an integrated multi-trophic aquaculture (IMTA) system was evaluated to obtain valuable products by a biorefinery approach. Enzymatic-assisted extraction (EAE) and microwave-assisted extraction (MAE) were the eco-friendly technologies used to ensure an environmentally friendly valorization of the biomass. Three valuable products were successfully recovered: a water-soluble extract rich in proteins and sulfated polysaccharides suitable as a food supplement; a lipid fraction rich in polyunsaturated fatty acids (PUFAs) with potential to be used in the nutraceutical ...
BioMed Research International, 2016
Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mex... more Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mexico (Macrocystis pyrifera,Eisenia arborea,Pelvetia compressa,Ulva intestinalis, andSolieria filiformis) were tested in this study in order to evaluate their effect on measles virusin vitro. All polysaccharides showed antiviral activity (as measured by the reduction of syncytia formation) and low cytotoxicity (MTT assay) at inhibitory concentrations. SPs fromEisenia arboreaandSolieria filiformisshowed the highest antiviral activities (confirmed by qPCR) and were selected to determine their combined effect. Their synergistic effect was observed at low concentrations (0.0274 μg/mL and 0.011 μg/mL ofE. arboreaandS. filiformisSPs, resp.), which exhibited by far a higher inhibitory effect (96% syncytia reduction) in comparison to the individual SP effects (50% inhibition with 0.275 μg/mL and 0.985 μg/mL ofE. arboreaandS. filiformis, resp.). Time of addition experiments and viral penetration as...
BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene ... more BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene un ancestro común, de manera que se les ha agrupado en razón de las similitudes que presentan. Para definirlas utilizamos, entonces, características que unifi-can al grupo, tales como la de ser organismos autótrofos que realizan la fotosíntesis oxigénica con niveles de organización y estructuras simples (carecen de flores, sistema vascular y raíces como las plantas), con pigmentos fotosintéticos y es-tructuras celulares similares. Debido a que la definición es muy general, en este conjunto se ha incluido al grupo de las cianofíceas o algas verde-azules (Cyanophyta), las cuales, aunque son procariontas, son consideradas en este grupo artificial en términos de sus pigmentos y su función en el ecosistema. Las especies de macroalgas son principalmente algas verdes (Chlorophyta), algas cafés (Phaeophyta), algas rojas (Rhodophyta), y algunas especies de cianofíceas o algas verde-azules (Cyanop...
Fucoidans are sulfated polysaccharides with proven pharmacological effects localized in the cell ... more Fucoidans are sulfated polysaccharides with proven pharmacological effects localized in the cell wall of marine brown algae. The majority of studies have been performed with temperate brown algal species, but in recent years, the evaluation of species from tropical areas has been growing. The aim of this study was to determine the protective effect of fucoidans extracted from the tropical brown seaweeds Dictyota ciliolata, Padina sanctae-crucis, and Sargassum fluitans, against oxidative stress (OS). The D. ciliolata fucoidan (FDc) exhibited the highest reactive oxygen species (ROS) scavenging activity (26%), followed by P. sanctaecrucis fucoidan (FPs) (22%) and S. fluitans fucoidan (FSf) (14%). No cytotoxic effect was detected for any of the extracted fucoidans at a concentration of 2 mg mL −1 . Not only did the fucoidans tested show protective effect against OS by reducing ROS generation, but they also increased the glutathione (GSH) level and restored catalase (CAT) activity. Fucoidans obtained from tropical seaweeds could be used as a potential natural ingredient for functional foods.
Phenolics, sometimes called polyphenolic compounds, are present in a variety of terrestrial and m... more Phenolics, sometimes called polyphenolic compounds, are present in a variety of terrestrial and marine plants. They are utilized as important components of both human and animal diets for their antioxidant capacity. More than 8000 phenolic structures are currently known (Bravo 1998). To date, most polyphenols isolated from marine sources and referenced in the literature are of macroalgal origin (Li et al. 2011). The structures of natural polyphenols vary from simple molecules, such as phenolic
acids and other simple polyphenolic compounds, to the more complex phlorotannins, which consist of polymeric structures made up of units of phloroglucinol (1,3,5-trihydroxybenzene), typically isolated from marine brown algae or Phaeophyceae (Harborne 1980; Ragan & Glombitza 1986). Polyphenols exhibit a wide range of biological effects as a consequence of their antioxidant properties. The relatively high concentration of phenolic compounds in marine algae species contributes
to their antioxidant properties, which can be of benefit in reducing oxidative reactions deleterious to health. Oxidative stress in the human cell occurs during disease conditions or when optimal nutrition is lacking. Under these circumstances, the so-called “reactive oxygen species” (ROS) are available to initiate lipid peroxidation and damage to other biomolecules. Current evidence strongly supports the contribution of phenolic compounds to the prevention of cardiovascular diseases (CVDs) and cancers,
and suggests their role in the prevention of neurodegenerative diseases and diabetes mellitus (Urquiaga & Leighton 2000). This chapter reviews some of the chemical and biological properties of marine algae phenolics as natural antioxidants and discusses their protective effects in biological systems and their potential
activities in food products.
Cellular Origin, Life in Extreme Habitats and Astrobiology Volume 16, 2011, DOI: 10.1007/978-90-4... more Cellular Origin, Life in Extreme Habitats and Astrobiology
Volume 16, 2011, DOI: 10.1007/978-90-481-9316-5
All Flesh Is Grass
Plant-Animal Interrelationships
Zvy Dubinsky and Joseph Seckbach
Se presenta una valiosa compilación de temas relacionado con la Biodiversidad de Yucatán, sus uso... more Se presenta una valiosa compilación de temas relacionado con la Biodiversidad de Yucatán, sus usos, amenazas y potencialidades, así como la importancia que ha tenido la riqueza biológica en el desarrollo de la sociedad y la historia de los habitantes de este Estado.
Este libro está conformado de 4 grandes apartados, 10 capítulos y 171 contribuciones temáticas con la participación de 250 autores, de 55 instituciones académicas.
Esta Obra constituye la compilación de información más actualizada de los distintos grupos biológicos que ocurren en Yucatán, incluyendo plantas, animales vertebrados e invertebrados, y microorganismos, lo cual nos permite tener una idea más clara del nivel de conocimiento que se tiene de la Diversidad Biológica de Estado. Además, nos acerca a conocer la diversidad de usos para los cuales se emplean los distintos organismos biológicos y sus partes en la elaboración de bienes que proveen, alimentos, medicinas, utensilios y materiales de construcción, así como energía, elementos todos que permiten a las poblaciones locales satisfacer algunas de sus necesidades más básicas. Asimismo, se presentan diversas actividades productivas del hombre relacionadas con biodiversidad, así como sus impactos sobre los ecosistemas del Estado.
Esperamos firmemente que esta obra, sobre la riqueza biológica de Yucatán y su relación con las actividades productivas del hombre, sea un importante instrumento educativo y de planeación para ayudar al desarrollo de políticas públicas en materia ambiental en el Estado.
The importance of seaweed resources in the country its not only due to species diversity and wide... more The importance of seaweed resources in the country its not only due to species diversity and wide geographical distribution, but also due to its economic importance. Until now most of the phycological studies have focused on the elaboration of taxonomic listing with the idea of having all the marine flora from the country registred. Nevertheless, in this way the knowledge of our seaweed resources its limited without having identified abundance and seasonality of the potentially economic species along the coast of Mexico. In this study we present the evaluation of economic important species from the Yucatan coast with emphasis to its potential use for human consumption and polysaccharide extraction for the food industry.
Journal of Experimental Botany, 2021
Journal of Experimental Botany, 2020
Short-term effects of pCO2 (700–380 ppm; High carbon (HC) and Low carbon (LC), respectively) and ... more Short-term effects of pCO2 (700–380 ppm; High carbon (HC) and Low carbon (LC), respectively) and nitrate content (50–5 µM; High nitrogen (HN) and Low nitrogen (LN), respectively on photosynthesis were investigated in Ulva rigida (Chlorophyta) under solar radiation (in-situ) and in the laboratory under artificial light (ex-situ). After six days of incubation at ambient temperature (AT), algae were subjected to a 4 °C temperature increase (AT+4 °C) for 3 d. Both in-situ and ex-situ maximal electron transport rate (ETRmax) and in situ gross photosynthesis (GP), measured by O2 evolution, presented highest values under HCHN, and lowest under HCLN, across all measuring systems. Maximal quantum yield (Fv/Fm), and ETRmax of photosystem (PS) II [ETR(II)max] and PSI [ETR(I)max], decreased under HCLN at AT+4 °C. Ex situ ETR was higher than in situ ETR. At noon, Fv/Fm decreased (indicating photoinhibition), whereas ETR(II)max and maximal non-photochemical quenching (NPQmax) increased. ETR(II)ma...
Hydrobiologia, 1991
... If the best commercial strategy for vegetative micropropagation is to maximize propagules per... more ... If the best commercial strategy for vegetative micropropagation is to maximize propagules per donor-plant ratios, the logical strategy would be to reduce ... Somatic-cell isolation in seaweeds is not a dif-ficult task as it has been achieved by partial enzymatic digestion of thalli (in ...
European Journal of Phycology, 1994
The filamentous brown algae Feldmannia simplex and F. irregular~ are attacked by aquatic viruses,... more The filamentous brown algae Feldmannia simplex and F. irregular~ are attacked by aquatic viruses, which propagate in deformed sporangia of the host plants. In order to evaluate a possible detrimental effect of the pathogen, photosynthesis-irradiance response curves and pigment concentrations of healthy and infected plants were compared. Photosynthetic performance of infected plants was significantly reduced and associated with a decrease in chlorophyll a and c content. In Feldmannia irregularis, which had a relatively low photosynthetic capacity, the virus infection produced a more severe effect than in F. simplex.
SPIE Proceedings, 2007
The green microalga Chlamydomonas reinhardtii is proposed to produce hydrogen in a low-cost syste... more The green microalga Chlamydomonas reinhardtii is proposed to produce hydrogen in a low-cost system using the solar radiation in Yucatan, Mexico. A two-step process is necessary with a closed photobioreactor, in which the algae are firstly growth and then induced for hydrogen generation. Preliminary results are presented in this work with some planning for the future. Different culture broths, temperatures and light intensities were tested for biomass and hydrogen production in laboratory conditions. The first experiments in external conditions with solar radiation and without temperature control have been performed, showing the potential of this technique at larger scales. However, some additional work must be done in order to optimize the culture maintenance, particularly in relation with the temperature control, the light radiation and the carbon dioxide supply, with the idea of keeping an economic production.
Journal of Applied Phycology, 2006
The effects of plant growth regulators on callus induction rate and regeneration of K. alvarezii ... more The effects of plant growth regulators on callus induction rate and regeneration of K. alvarezii explants was evaluated. K. alvarezii calluses were induced in vitro with kinetin (K), 6-benzylaminopurine (B), 1naphtalene acetic acid (N) and spermine (S). After 30 days, K. alvarezii explants produced filamentous calluses and isolated crystalline filaments growing from the medullar region and from cortical cells at the cut edge. The plant growth regulators 1-naphtalene acetic acid (1 mg L −1) and 6-benzylaminopurine (1 mg L −1) and the 1-naphtalene acetic acid + kinetin + spermine (1, 1, 0.018 mg L −1 respectively) combination produced 85 to 129% more calluses, with significant differences versus the control (p < 0.05). Spermine at 0.018 mg L −1 produced calluses in the apical, intercalary and basal regions of explants. Spermine also reduced callus induction time to 7 days, which is faster than previously reported induction times with other plant growth regulators. An airlift bioreactor was designed and characterized to micropropagate K. alvarezii calluses. The bioreactor had mixing times ranging from 4.6-10.3 s at T 90 and T 95 , which is shorter than those for the
Marine Drugs, 2022
Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. ... more Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC50 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC50 of 0.38 μg/mL during the...
Phycologia, 2019
Seaweed farming has now expanded across several continents from South East Asia to South America,... more Seaweed farming has now expanded across several continents from South East Asia to South America, Northern Europe, Canada and East Africa, contributing to global food security, supporting rural livelihoods, alleviating poverty and improving the health of oceans. Latin America (LA) covers a vast geographical area, which includes four different oceanic domains (Temperate Northern Pacific, Tropical Eastern Pacific, Temperate South America and Tropical Atlantic) and encompasses many types of coastal ecosystems with a wide range of seaweed species. LA has major potential for the development of seaweed farming activities; however, almost all the production is based on the harvesting of natural beds. This review describes the development of and prospects for the aquaculture seaweed industry in LA. The status of the seaweed aquaculture sector for green, brown and red seaweed and the main industry challenges are addressed. Regulation in the primary countries is also discussed. The expansion of the aquaculture industry in this region can be improved with new strains and farming methodologies, diversification of species, market expansion and an increase in domestic demand.
Natural Product Communications, 2017
Polysaccharides extracted from Rhodymenia pseudopalmata, Solieria filiformis, Hydropuntia cornea ... more Polysaccharides extracted from Rhodymenia pseudopalmata, Solieria filiformis, Hydropuntia cornea (Rhodophyta) and Sargassum fluitans (Phaeophyceae) were evaluated for its cytotoxic and antiviral activities against Herpes simplex virus (HSV-Type 1). Chemical structures were characterized by FT-IR spectroscopy and 13C-NMR analyses. Polysaccharides from Sargassum fluitans (EC50 = 42.8 μg/ml) and Solieria filiformis (EC50 = 136.0 μg/ml) showed antiviral activity against herpes simplex virus type-I in vitro at a multiplicity of infection (MOI) of 0.01 ID50/cells without cytotoxicity (1–200 μg/mL). The activity observed suggests that sulphation, molecular weight and carbohydrate nature of these polysaccharides may be involved in this activity. To better understand the antiviral activity of the polysaccharides evaluated, it seems important to study the mechanism of action involved. These polysaccharides could be studied further to evaluate their potential use as antiviral drugs.
Marine Drugs, 2018
Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefine... more Marine macroalgae (seaweed) are an excellent source of novel bioactive metabolites. The biorefinery concept applied to seaweed facilitates the extraction of many chemical constituents from the same biomass ensuring that the resource is used fully, generating few residues through a succession of extraction steps. In the present study, the biomass of the carragenophyte Solieria filiformis (Rhodophyta, Gigartinales) cultured in an integrated multi-trophic aquaculture (IMTA) system was evaluated to obtain valuable products by a biorefinery approach. Enzymatic-assisted extraction (EAE) and microwave-assisted extraction (MAE) were the eco-friendly technologies used to ensure an environmentally friendly valorization of the biomass. Three valuable products were successfully recovered: a water-soluble extract rich in proteins and sulfated polysaccharides suitable as a food supplement; a lipid fraction rich in polyunsaturated fatty acids (PUFAs) with potential to be used in the nutraceutical ...
BioMed Research International, 2016
Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mex... more Sulfated polysaccharides (SPs) extracted from five seaweed samples collected or cultivated in Mexico (Macrocystis pyrifera,Eisenia arborea,Pelvetia compressa,Ulva intestinalis, andSolieria filiformis) were tested in this study in order to evaluate their effect on measles virusin vitro. All polysaccharides showed antiviral activity (as measured by the reduction of syncytia formation) and low cytotoxicity (MTT assay) at inhibitory concentrations. SPs fromEisenia arboreaandSolieria filiformisshowed the highest antiviral activities (confirmed by qPCR) and were selected to determine their combined effect. Their synergistic effect was observed at low concentrations (0.0274 μg/mL and 0.011 μg/mL ofE. arboreaandS. filiformisSPs, resp.), which exhibited by far a higher inhibitory effect (96% syncytia reduction) in comparison to the individual SP effects (50% inhibition with 0.275 μg/mL and 0.985 μg/mL ofE. arboreaandS. filiformis, resp.). Time of addition experiments and viral penetration as...
BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene ... more BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene un ancestro común, de manera que se les ha agrupado en razón de las similitudes que presentan. Para definirlas utilizamos, entonces, características que unifi-can al grupo, tales como la de ser organismos autótrofos que realizan la fotosíntesis oxigénica con niveles de organización y estructuras simples (carecen de flores, sistema vascular y raíces como las plantas), con pigmentos fotosintéticos y es-tructuras celulares similares. Debido a que la definición es muy general, en este conjunto se ha incluido al grupo de las cianofíceas o algas verde-azules (Cyanophyta), las cuales, aunque son procariontas, son consideradas en este grupo artificial en términos de sus pigmentos y su función en el ecosistema. Las especies de macroalgas son principalmente algas verdes (Chlorophyta), algas cafés (Phaeophyta), algas rojas (Rhodophyta), y algunas especies de cianofíceas o algas verde-azules (Cyanop...
BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene ... more BIODIVERSIDAD 162 Definir las algas no es una tarea fácil pues se trata de un grupo que no tiene un ancestro común, de manera que se les ha agrupado en razón de las similitudes que presentan. Para definirlas utilizamos, entonces, características que unifi-can al grupo, tales como la de ser organismos autótrofos que realizan la fotosíntesis oxigénica con niveles de organización y estructuras simples (carecen de flores, sistema vascular y raíces como las plantas), con pigmentos fotosintéticos y es-tructuras celulares similares. Debido a que la definición es muy general, en este conjunto se ha incluido al grupo de las cianofíceas o algas verde-azules (Cyanophyta), las cuales, aunque son procariontas, son consideradas en este grupo artificial en términos de sus pigmentos y su función en el ecosistema. Las especies de macroalgas son principalmente algas verdes (Chlorophyta), algas cafés (Phaeophyta), algas rojas (Rhodophyta), y algunas especies de cianofíceas o algas verde-azules (Cyanop...
Aquaculture Economics & Management, 2015
The accuracy of the Content should not be relied upon and should be independently verified with p... more The accuracy of the Content should not be relied upon and should be independently verified with primary sources of information. Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. This article may be used for research, teaching, and private study purposes. Any substantial or systematic reproduction, redistribution, reselling, loan, sub-licensing, systematic supply, or distribution in any form to anyone is expressly forbidden.
Seasonal variation of fatty acid composition of Halymenia floresii (Rhodophyta algae) was determi... more Seasonal variation of fatty acid composition of Halymenia floresii (Rhodophyta algae) was determined by conventional methods and gas chromatography-mass spectrometry (GC-MS). Three saturated fatty acids (SAFA) were identified (C14:0, C16:0, C18:0) and a monounsaturated (MUFA) C18:1 Δ9. Results indicated a cons-tant SAFA/MUFA proportion throughout the year (dry and rainy season), except in the windy season where MUFA increased and particularly oleic acid. In general, H. floresii has a good supply of fatty acids compared with commercial algae such as nori (Porphyra sp.) and dulse (Palmaria palmata). www.relaquim.com
Sixteenth International Seaweed Symposium, 1999
The yield and gel properties of agar from Gelidium robustum, harvested in Baja California for ind... more The yield and gel properties of agar from Gelidium robustum, harvested in Baja California for industrial production is affected by season of collection and epiphyte loading. The alga is epiphytized to various extents by the bryozoan Membraniphora tuberculata ('conchilla') and the resulting calcareous crust on the alga diminishes the price of the seaweed biomass. Classification of the algal biomass quality by the agar industry is based on the apparent 'conchilla' content from visual examination. The different quality classes can be categorized quantitatively into premium class (30-40% w/w of 'conchilla' load), 2nd class (∼ 50% w/w) and 3rd class (> 60% w/w). For samples collected at two exploited beds, the biomass obtained from Bahía de Tortugas had a lower epiphytic coverage than that from Bahía Asunción. The agar yield from different quality classes of G. robustum was strongly affected by the bryozoan epiphytic coverage, while its gel characteristics were not. Algae collected at Punta Prieta, an unexploited bed not affected by 'conchilla', showed seasonality in agar yield. It ranged between 17.5 and 44.2% with two maximum values observed, one in summer and the other in winter. Gel strength ranged between 515 and 665 g cm −2 , reaching a maximum during autumn.
The effect of alkali treatments on the yield, rheological and chemical properties of agar from Gr... more The effect of alkali treatments on the yield, rheological and chemical properties of agar from Gracilaria cornea growing along the Yucatán coast were studied in order to evaluate its potential for industrial use in an attractive economic standpoint. Alkali treatment was carried out with NaOH concentrations of 0.5%, 1%, 3% and 5% in a water bath at 80, 85 and 90 C. Agar yield, gel strength, gelling and melting temperatures, sulphate, 3,6-anhydrogalactose and ash content were determined. The different combinations of NaOH concentration and treatment temperature strongly influenced agar characteristics. There was a variation in the agar content for all NaOH treatments and temperature combinations, ranging between 14.5% to 22.1%. Although the yields obtained for 0.5% NaOH at all temperatures and 1% NaOH at 80 and 85 C were higher than those required by the industry, the physical and chemical characteristics of the agar were similar to those obtained for native agar from the same species. The gel strengths, sulphate content and gelation hysteresis obtained with agar from the 1% NaOH treatment at 90 C are in the range required by the food industry. Treatments with 3% and 5% NaOH at all temperatures improved significantly the agar quality giving higher gel strengths (974-1758 g cm 2) than those reported for other Gracilaria species.
Agar films were subjected to natural weathering exposure in a humid tropical climate for 90 days ... more Agar films were subjected to natural weathering exposure in a humid tropical climate for 90 days to determine their biodegradation behavior and functionality. Exposed samples were taken at 15, 30, 45, 60 and 90 days. Mechanical, thermal, structural and morphological properties were determined using tensile test, differential scanning calorimetry (DSC), attenuated reflectance infrared spectroscopy (ATR-FTIR), X-ray diffraction and environmental scanning electron microscopy (ESEM). The photodegradation process and temperatureerelative humidity fluctuations promoted a decrease in agar mechanical properties in early exposure stages (30e45 days) caused by a reduction in agar molecular size and a decrease in the number of sulfate groups. These changes alter agar crystallinity, causing contraction that leads to formation of micro-fractures and embrittlement, and promote microbial attack. Accelerated weathering exposure of agar films showed that outdoor climate parameters play an important role in their degradation. These results will aid in further research to determine the potential use of agar as an environmentally friendly solution to the problem of biodegradable composites disposal.
Sixteenth International Seaweed Symposium, 1999
The two main permanent magnet technologies (hybrid and pure permanent magnets) are compared for i... more The two main permanent magnet technologies (hybrid and pure permanent magnets) are compared for invacuum undulator design. The small ratio gap/period favours the hybrid technology despite of the difficult passive field termination design. The choice of the permanent magnet material is an issue. Based on non linear permanent magnet models, simulations using RADIA are presented. The material intrinsic coercivity H cj is determined for both NdFeB and Sm 2 Co 17 material to avoid any irreversible demagnetisation during the required baking (≤140 deg. C) of the undulator. The resulting commercially available material are compared. The status of ESRF in-vacuum undulators is presented. Four devices have been completed and the main magnetic measurement results are summarized. In particular, the spectrum shimming performances are discussed. The construction of additional devices has started.