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TY - CHAP AU - Cangalaya, Carla AU - Dityatev, Alexander ED - Rusakov, Dmitri PY - 2024 DA - 2024// TI - Synaptic Photodamage and Its Application to Study Microglia–Synapse Interactions In Vivo BT - Fluorescence Imaging of the Brain SP - 111 EP - 126 PB - Springer US CY - New York, NY AB - Microglia appeared as the major player in the remodeling of synapses under physiological and pathophysiological conditions, in particular during development, forgetting, and neuroinflammation. In this chapter, we describe in vivo two-photon photodamage of single synapses to induce microglia–synapse interactions. Application examples are provided to illustrate the advantages offered by this methodology for the spatiotemporal control of microglia engagement in synaptic remodeling. Direct observation of this process opens new possibilities to gain mechanistic insights into how microglia are involved in various neurological and psychiatric disorders. SN - 978-1-0716-4011-1 UR - https://doi.org/10.1007/978-1-0716-4011-1\_5 DO - 10.1007/978-1-0716-4011-1_5 ID - Cangalaya2024 ER -