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TY - JOUR AU - Harrison, G. E. AU - Heslop-Harrison, J. S. PY - 1995 DA - 1995/02/01 TI - Centromeric repetitive DNA sequences in the genus Brassica JO - Theoretical and Applied Genetics SP - 157 EP - 165 VL - 90 IS - 2 AB - Representatives of two major repetitive DNA sequence families from the diploid Brassica species B. campestris and B. oleracea were isolated, sequenced and localized to chromosomes by in situ hybridization. Both sequences were located near the centromeres of many chromosome pairs in both diploid species, but major sites of the two probes were all on different chromosome pairs. Such chromosome specificity is unusual for plant paracentromeric repetitive DNA. Reduction of stringency of hybridization gave centromeric hybridization sites on more chromosomes, indicating that there are divergent sequences present on other chromosomes. In tetraploid species derived from the diploids, the number of hybridization sites was different from the sum of the diploid ancestors, and some chromosomes had both sequences, indicating relatively rapid homogenization and copy number evolution since the origin of the tetraploid species. SN - 1432-2242 UR - https://doi.org/10.1007/BF00222197 DO - 10.1007/BF00222197 ID - Harrison1995 ER -