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TY - JOUR AU - McGraw, Bonnie R. AU - Marinus, M. G. PY - 1980 DA - 1980/05/01 TI - Isolation and characterization of dam+ revertants and suppressor mutations that modify secondary phenotypes of dam-3 strains of Escherichia coli K-12 JO - Molecular and General Genetics MGG SP - 309 EP - 315 VL - 178 IS - 2 AB - Bacteria mutant in the dam (DNA adenine methylation) gene and in either recA or recB or recC genes are inviable (Virm- phenotype). From crosses between dam-3 bacteria and recA1 or recB21 recC22 strains, Vrm+ recombinants were recovered. Among these recombinants, Dam+ revertants were present which did not show the phenotypes normally associated with dam-3 bacteria. Three classes of indirectly suppressed strains (dam-3 genotype) were also recovered which showed alterations in the secondary phenotypes normally associated with dam-3 bacteria. These strains contained a second unlinked mutation in either mutL or mutS or sin. In addition, mutation in either sbcA or sbcB suppresses the Vrm- phenotype of dam-3 recB21 recC22 strains. SN - 1432-1874 UR - https://doi.org/10.1007/BF00270477 DO - 10.1007/BF00270477 ID - McGraw1980 ER -