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TY - JOUR AU - Stettler, Sophie AU - Chiannilkulchai, Nuchanard AU - Hermann-Le Denmat, Sylvie AU - Lalo, Dominique AU - Lacroute, François AU - Sentenac, André AU - Thuriaux, Pierre PY - 1993 DA - 1993/05/01 TI - A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae JO - Molecular and General Genetics MGG SP - 169 EP - 176 VL - 239 IS - 1 AB - A multicopy genomic library of Saccharomyces cerevisiae (strain FL100) was screened for its ability to suppress conditionally defective mutations altering the 31 kDa subunit (rpc31–236) or the 53 kDa subunit (rpc53-254/424) of RNA polymerase III. In addition to allele-specific suppressors, we identified seven suppressor clones that acted on both mutations and also suppressed several other conditional mutations defective in RNA polymerases I or II. All these clones harbored a complete copy of the SSD1 gene. The same pleiotropic suppression pattern was found with the dominant SSD1-v allele present in some laboratory strains of S. cerevisiae. SSD1-v was previously shown to suppress mutations defective in the SIT4 gene product (a predicted protein phosphatase subunit) or in the regulatory subunit of the cyclic AMP-dependent protein kinase. We propose that the SSD1 gene product modulates the activity (or the level) of the three nuclear RNA polymerases, possibly by altering their degree of phosphorylation. SN - 1432-1874 UR - https://doi.org/10.1007/BF00281615 DO - 10.1007/BF00281615 ID - Stettler1993 ER -