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TY - JOUR AU - Bugawan, Teodorica L. AU - Erlich, Henry A. PY - 1991 DA - 1991/05/01 TI - Rapid typing of HLA-DQB1 DNA polymorphism using nonradioactive oligonucleotide probes and amplified DNA JO - Immunogenetics SP - 163 EP - 170 VL - 33 IS - 3 AB - The allelic sequence diversity at theHLA-DQBI locus has been analyzed by polymerase chain reaction (PCR) amplification and sequencing. Fifteen amino acid sequence-defined alleles (one previously unreported) and several silent nucleotide polymorphisms which subdivide these alleles have been identified. Here, we describe the specific amplification of theDQB1 second exon by several different PCR primer pairs and a simple and rapid typing procedure using a panel of 16 horseradish peroxidase (HRP)-labeled oligonucleotide probes capable of distinguishing theseDQBI alleles. SN - 1432-1211 UR - https://doi.org/10.1007/BF01719235 DO - 10.1007/BF01719235 ID - Bugawan1991 ER -