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TY - JOUR AU - Mai, Sabine AU - Fluri, Monika AU - Siwarski, David AU - Huppi, Konrad PY - 1996 DA - 1996/08/01 TI - Genomic instability in MycER-activated Rat1A-MycER cells JO - Chromosome Research SP - 365 EP - 371 VL - 4 IS - 5 AB - The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged periods. To this end, we have used Rat1A-MycER cells, which allow the experimental regulation of Myc protein levels. We examined the genomic stability of Rat1A-MycER cells cultivated in either the absence or the presence of estrogen, which reportedly activates the chimeric MycER protein in these cells. Following prolonged periods of MycER activation, Rat1A-Mycer cells exhibited irreversible chromosomal aberrations. The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements. SN - 1573-6849 UR - https://doi.org/10.1007/BF02257272 DO - 10.1007/BF02257272 ID - Mai1996 ER -