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TY - JOUR AU - Rizk, Sandra E. AU - Abdel-Massih, Roula M. AU - Baydoun, Elias A.-H. AU - Brett, Christopher T. PY - 2000 DA - 2000/08/01 TI - Protein- and pH-dependent binding of nascent pectin and glucuronoarabinoxylan to xyloglucan in pea JO - Planta SP - 423 EP - 429 VL - 211 IS - 3 AB - Nascent pectin and glucuronoarabinoxylan, synthesised in vitro by membrane-bound enzymes from etiolated pea (Pisum sativum L.) epicotyls, were found to bind to pea xyloglucan in a pH-dependent manner. The binding was maximum at low pH (3–4), and decreased to almost zero at pH 6. The binding was probably non-covalent and reached saturation within 5 min. Removal of the fucose residues of xyloglucan decreased the degree of binding. Removal by protease of the proteins attached to nascent pectin and glucuronoarabinoxylan greatly reduced the maximum binding and abolished the pH-dependence. The observed binding may be of considerable significance in the process of cell-wall assembly and in the control of cell extension. SN - 1432-2048 UR - https://doi.org/10.1007/s004250000303 DO - 10.1007/s004250000303 ID - Rizk2000 ER -