(original) (raw)

TY - JOUR AU - Zhang, Xiaoai AU - Xu, Hongmei AU - Chen, Xiaodan AU - Li, Xiujun AU - Wang, Xianjun AU - Ding, Shujun AU - Zhang, Renli AU - Liu, Lijuan AU - He, Cui AU - Zhuang, Lu AU - Li, Hao AU - Zhang, Panhe AU - Yang, Hong AU - Li, Tingyu AU - Liu, Wei AU - Cao, Wuchun PY - 2014 DA - 2014/02/01 TI - Association of functional polymorphisms in the MxA gene with susceptibility to enterovirus 71 infection JO - Human Genetics SP - 187 EP - 197 VL - 133 IS - 2 AB - Myxovirus resistance A (MxA) is an antiviral protein induced by type I interferons α and β (IFN-α and IFN-β) that can inhibit virus replication. We examined whether the MxA polymorphisms were related to the risk and severity of enterovirus 71 (EV71) infection in Chinese populations. The MxA C-123A and G-88T polymorphisms were genotyped in two independent case–control populations in China by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis. Multivariate logistic regression analysis was used to calculate adjusted odds ratios (ORs) and 95 % confidence intervals (95 % CIs). MxA messenger RNA was quantified by real-time quantitative PCR in peripheral blood mononuclear cells (PBMCs) from 45 healthy children and 19 patients with EV71 infection. Significantly decreased susceptibility to EV71 infection was observed for the -123A allele and -88T allele carriers, with ORs (95 % CIs) estimated as 0.56 (0.39–0.81) and 0.64 (0.47–0.88), respectively, in the northern population. This association was confirmed in the southern population, with ORs (95 % CIs) estimated as 0.58 (0.38–0.89) and 0.67(0.47–0.95), respectively. The A-123T-88 haplotype was also significantly associated with lower risk of EV71 infection in both the northern (OR = 0.62; 95 % CI = 0.44–0.85) and the southern population (OR = 0.63; 95 % CI = 0.43–0.92). Furthermore, we observed higher MxA messenger RNA levels in IFNβ1a-stimulated PBMCs from the -123A or -88T allele carriers compared with that from nocarriers. Our findings suggest that polymorphisms in the MxA promoter may play a role in mediating the susceptibility to EV71 infection in Chinese population. SN - 1432-1203 UR - https://doi.org/10.1007/s00439-013-1367-3 DO - 10.1007/s00439-013-1367-3 ID - Zhang2014 ER -