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TY - JOUR AU - Broide, David H. AU - Stachnick, Greg AU - Castaneda, Diego AU - Nayar, Jyothi AU - Miller, Marina AU - Cho, Jae Youn AU - Roman, Mark AU - Zubeldia, Jose AU - Hyashi, Tomoko AU - Raz, Eyal PY - 2001 DA - 2001/05/01 TI - Systemic Administration of Immunostimulatory DNA Sequences Mediates Reversible Inhibition of Th2 Responses in a Mouse Model of Asthma JO - Journal of Clinical Immunology SP - 175 EP - 182 VL - 21 IS - 3 AB - This study investigated whether immunostimulatory DNA sequences (ISS) induce a transient or sustained inhibition of Th2 responses to inhaled antigen. We sensitized mice with subcutaneous injections to develop a Th2 response to ovalbumin (ova) and then administered a dose of ISS prior to ova inhalation challenge. Mice were then rechallenged with ova by inhalation a second time at varying time points after the first ova inhalation (1 to 8 weeks later) to determine whether the ISS dose administered prior to the first ova inhalation protected against a subsequent second ova inhalation challenge. A single dose of ISS inhibited the Th2 response to the first inhalation of ova antigen, as well as 4 weeks later to the second inhalation of ova. However, ISS did not inhibit a Th2 response to the second inhalation of ova 8 weeks later. The reversible inhibition of Th2 responses at 8 weeks suggests the need for repeated ISS administration at monthly intervals. SN - 1573-2592 UR - https://doi.org/10.1023/A:1011078930363 DO - 10.1023/A:1011078930363 ID - Broide2001 ER -