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TY - JOUR AU - Labib, Karim AU - Diffley, John F.X. AU - Kearsey, Stephen E. PY - 1999 DA - 1999/11/01 TI - G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus JO - Nature Cell Biology SP - 415 EP - 422 VL - 1 IS - 7 AB - Cyclin-dependent kinases (CDKs) activate the firing of replication origins during the S phase of the cell cycle. They also block re-initiation of DNA replication within a single cell cycle, by preventing the assembly of prereplicative complexes at origins. We show here that, in budding yeast, CDKs exclude the essential prereplicative-complex component Mcm4 from the nucleus. Although origin firing can be triggered by the B-type cyclins only, both G1-phase and B-type cyclins cause exit of Mcm4 from the nucleus. These results suggest that G1 cyclins may diminish the cell’s capacity to assemble prereplicative complexes before B-type cyclins trigger origin firing during S phase. SN - 1476-4679 UR - https://doi.org/10.1038/15649 DO - 10.1038/15649 ID - Labib1999 ER -