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TY - JOUR AU - GALFRE, G. AU - HOWE, S. C. AU - MILSTEIN, C. AU - BUTCHER, G. W. AU - HOWARD, J. C. PY - 1977 DA - 1977/04/01 TI - Antibodies to major histocompatibility antigens produced by hybrid cell lines JO - Nature SP - 550 EP - 552 VL - 266 IS - 5602 AB - FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2. One of the most powerful features of this approach is that, by cloning, one may easily derive cell lines synthesising monoclonal antibodies despite using non-purified immunogens. The multiple components of a heterogeneous population of hybrid cells are resolved by cloning techniques. This feature makes the system a very powerful tool in the study of complex antigenic structures. The established cell lines offer the further advantage of unlimited permanent supply of material, and the possibility of worldwide standardisation. SN - 1476-4687 UR - https://doi.org/10.1038/266550a0 DO - 10.1038/266550a0 ID - GALFRE1977 ER -