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TY - JOUR AU - Downes, Peter AU - Michell, Robert H. PY - 1981 DA - 1981/03/01 TI - Human erythrocyte membranes exhibit a cooperative calmodulin-dependent Ca2+-ATPase of high calcium sensitivity JO - Nature SP - 270 EP - 271 VL - 290 IS - 5803 AB - It is thought that the ionized Ca2+ concentration in the cytosol of healthy erythrocytes is in the range 0.01–0.1 µM (ref. 1) and that this low concentration is maintained by an ATP-driven calmodulin-dependent Ca2+ pump in the plasma membrane2,3. The Ca2+-stimulated ATPase which is the enzymatic expression of this pump varies in its calcium sensitivity between different preparations of erythrocyte ghosts, with activation generally occurring in a concentration range between ∼1 and 10 µM Ca2+ (refs 2–5). This is a higher range of Ca2+ concentrations than might be anticipated for activation of a pump that sustains intracellular concentrations of Ca2+ below 0.1 µM, and recent reports have suggested activation in some membrane preparations at Ca2+ concentrations in the range 0.1–1.0 µM (refs 6, 7). We report here a simple method for preparing human erythrocyte membranes in 2.5 mM HEPES/1 mM EGTA at pH 7.0 (see Fig. 1 legend) in which the activation of the Ca2+-ATPase by Ca2+ and intracellular concentrations of calmodulin is highly cooperative and is complete by ∼1 µM Ca2+. Unlike other available erythrocyte membrane preparations, the pattern of activation by Ca2+ and calmodulin is not complicated by partial resealing of the ghosts during and after isolation. We suggest that this cooperative activation of the Ca2+ pump may explain how healthy erythrocytes maintain their normal cytosol Ca2+ concentration at a threshold value at or below ∼0.1 µM. We also note that several other calmodulin-dependent enzymes display similar cooperative activation kinetics. SN - 1476-4687 UR - https://doi.org/10.1038/290270a0 DO - 10.1038/290270a0 ID - Downes1981 ER -