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TY - JOUR AU - Smith, Lloyd M. AU - Sanders, Jane Z. AU - Kaiser, Robert J. AU - Hughes, Peter AU - Dodd, Chris AU - Connell, Charles R. AU - Heiner, Cheryl AU - Kent, Stephen B. H. AU - Hood, Leroy E. PY - 1986 DA - 1986/06/01 TI - Fluorescence detection in automated DNA sequence analysis JO - Nature SP - 674 EP - 679 VL - 321 IS - 6071 AB - We have developed a method for the partial automation of DNA sequence analysis. Fluorescence detection of the DNA fragments is accomplished by means of a fluorophore covalently attached to the oligonucleotide primer used in enzymatic DNA sequence analysis. A different coloured fluorophore is used for each of the reactions specific for the bases A, C, G and T. The reaction mixtures are combined and co-electrophoresed down a single polyacrylamide gel tube, the separated fluorescent bands of DNA are detected near the bottom of the tube, and the sequence information is acquired directly by computer. SN - 1476-4687 UR - https://doi.org/10.1038/321674a0 DO - 10.1038/321674a0 ID - Smith1986 ER -