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TY - JOUR AU - Maryanski, Janet L. AU - Pala, Pietro AU - Corradin, Giampietro AU - Jordan, Bertrand R. AU - Cerottini, Jean-Charles PY - 1986 DA - 1986/12/01 TI - H—2-restricted cytolytic T cells specific for HLA can recognize a synthetic HLA peptide JO - Nature SP - 578 EP - 579 VL - 324 IS - 6097 AB - It is generally accepted that T lymphocytes recognize antigens in the context of molecules encoded by genes in the major histocompatibility complex (MHC). MHC class Il-restricted T cells usually recognize degraded or denatured rather than native forms of antigen on the surface of class II-bearing antigen presenting cells (reviewed in refs l, 2). It has recently been shown3 that short synthetic peptides corresponding to mapped antigenic sites of the influenza nucleoprotein (NP) can render uninfected target cells susceptible to lysis by NP-specific class I-restricted cytolytic T cells (CTL). These and earlier experiments4 that showed specific recognition of NP deletion mutant transfectants suggest that class I-restricted recognition might also involve processed antigenic fragments. One important issue arising from these studies is whether the model applies not only to viral proteins that are expressed internally (such as NP) but also to antigens normally expressed as integral membrane proteins at the cell surface. We have recently isolated class I-restricted mouse CTL clones that recognize class I gene products of the human MHC (HLA) as antigens in mouse cell HLA-transfectants5. Here we show that these anti-HLA CTL can lyse HLA-negative syngeneic mouse cells in the presence of a synthetic HLA peptide. These results suggest that the model applies generally. SN - 1476-4687 UR - https://doi.org/10.1038/324578a0 DO - 10.1038/324578a0 ID - Maryanski1986 ER -