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TY - JOUR AU - Reading, Donald S. AU - Hallberg, Richard L. AU - Myers, Alan M. PY - 1989 DA - 1989/02/01 TI - Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor JO - Nature SP - 655 EP - 659 VL - 337 IS - 6208 AB - The hsp60 protein isolated from the protozoan Tetrahymena thermophila is induced in response to heat stress and is a member of an immunologically conserved family represented in Escherichia coli and in mitochondria of plants and animals1,2. We report here the cloning and characterization of a nuclear gene, HSP60, which codes for the hsp60 homologue from the yeast Saccharomyces cerevisiae. Nucleotide sequence analysis revealed that yeast hsp60 is related to the groEL protein of E. coli3-6 and the RUBISCO-binding protein (RBP) of chloroplasts7. HSP60 was found to be the genetic locus of the conditional-lethal mutation described by Cheng et al.8, which at non-permissive temperature is defective in the assembly of several different multisubunit complexes in mitochondria. These data are consistent with the hypothesis that the groEL-related proteins serve an evolutionarily conserved function as accessory factors facilitating the folding and/or association of individual subunits of multimeric protein complexes7,9-14. SN - 1476-4687 UR - https://doi.org/10.1038/337655a0 DO - 10.1038/337655a0 ID - Reading1989 ER -