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TY - JOUR AU - Antonny, Bruno AU - Madden, David AU - Hamamoto, Susan AU - Orci, Lelio AU - Schekman, Randy PY - 2001 DA - 2001/06/01 TI - Dynamics of the COPII coat with GTP and stable analogues JO - Nature Cell Biology SP - 531 EP - 537 VL - 3 IS - 6 AB - We have developed an assay to monitor the assembly of the COPII coat onto liposomes in real time. We show that with Sar1pGTP bound to liposomes, a single round of assembly and disassembly of the COPII coat lasts a few seconds. The two large COPII complexes Sec23/24p and Sec13/31p bind almost instantaneously (in less than 1 s) to Sar1pGTP-doped liposomes. This binding is followed by a fast (less than 10 s) disassembly due to a 10-fold acceleration of the GTPase-activating protein activity of Sec23/24p by the Sec13/31p complex. Experiments with the phosphate analogue BeFx suggest that Sec23/24p provides residues directly involved in GTP hydrolysis on Sar1p. SN - 1476-4679 UR - https://doi.org/10.1038/35078500 DO - 10.1038/35078500 ID - Antonny2001 ER -