(original) (raw)

TY - JOUR AU - Randriamampita, Clotilde AU - Tsien, Roger Y. PY - 1993 DA - 1993/08/01 TI - Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx JO - Nature SP - 809 EP - 814 VL - 364 IS - 6440 AB - INTRACELLULAR Ca2+ signals that last more than a few minutes after the onset of stimulation depend critically on influx of extracellular Ca2+. Such Ca2+ influx can be triggered in many cell types by depletion of intracellular Ca2+ stores without detectable elevations of known messengers. The mechanism by which store depletion can control plasma membrane Ca2 + permeability remains controversial1–7. Here we present evidence for a novel soluble mediator. Calcium depletion of a lymphocyte cell line caused the messenger to be released from intracellular organelles into the cytoplasm and to a much lesser extent into the extracellular medium. The messenger caused Ca2+ influx when applied to macrophages, astrocytoma cells, and flbroblasts and was therefore named CIF (for Ca2+-influx factor). CIF appears to have hydroxyls (or hydroxyl and amino groups) on adjacent carbons, a phosphate, and a Mr under 500. SN - 1476-4687 UR - https://doi.org/10.1038/364809a0 DO - 10.1038/364809a0 ID - Randriamampita1993 ER -