(original) (raw)

TY - JOUR AU - Mäkelä, Tomi P. AU - Tassan, Jean-Pierre AU - Nigg, Erich A. AU - Frutiger, Séverine AU - Hughes, Graham J. AU - Weinberg, Robert A. PY - 1994 DA - 1994/09/01 TI - A cyclin associated with the CDK-activating kinase MO15 JO - Nature SP - 254 EP - 257 VL - 371 IS - 6494 AB - THE eukaryotic cell cycle is regulated by the sequential activation of cyclin-dependent kinases (CDKs)1. CDK activation is dependent on cyclin binding2–4 and phosphorylation of a conserved threonine (T161 in Cdc2)5–9 mediated by the CDK-activating kinase CAK9. A CDK-related kinase, MO15 (ref. 10), has been identified as the catalytic subunit of CAK (refs 11–13). Here we use a yeast two-hybrid screen to show that a new human cyclin (cyclin H) is a MO15-associated protein. Cyclin H is a major MO15 partner in vivo and enhances the kinase activity of MO15 towards Cdk2/cyclin A. These findings demonstrate that a cyclin/kinase complex can function as a regulator of other cyclin/kinase complexes, and suggest that cyclin/kinase cascades may exist. SN - 1476-4687 UR - https://doi.org/10.1038/371254a0 DO - 10.1038/371254a0 ID - Mäkelä1994 ER -