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TY - JOUR AU - Collignon, Jérôme AU - Varlet, Isabella AU - Robertson, Elizabeth J. PY - 1996 DA - 1996/05/01 TI - Relationship between asymmetric nodal expression and the direction of embryonic turning JO - Nature SP - 155 EP - 158 VL - 381 IS - 6578 AB - GROWTH factors related to TGF-β provide important signals for patterning the vertebrate body plan1–3. One such family member, nodal, is required for formation of the primitive streak during mouse gastrulation4–6. Here we have used a nodal–lacZ reporter allele to demonstrate asymmetric nodal expression in the mouse node, a structure thought to be the functional equivalent of the frog and chick 'organizer'7, and in lateral plate mesoderm cells. We have also identified two additional genes acting with nodal in a pathway determining the left–right body axis. Thus we observe in inv mutant embryos8 that the sidedness of nodal expression correlates with the direction of heart looping and embryonic turning. In contrast, HNF3-β+/- nodallacZ/+ double-heterozygous embryos display LacZ staining on both left and right sides, and frequently exhibit defects in body situs. Taken together, these experiments, along with similar findings in chick9, demonstrate that elements of the genetic pathway that establish the left–right body axis are conserved in vertebrates. SN - 1476-4687 UR - https://doi.org/10.1038/381155a0 DO - 10.1038/381155a0 ID - Collignon1996 ER -