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TY - JOUR AU - Lin, Xiaoying AU - Kaul, Samir AU - Rounsley, Steve AU - Shea, Terrance P. AU - Benito, Maria-Ines AU - Town, Christopher D. AU - Fujii, Claire Y. AU - Mason, Tanya AU - Bowman, Cheryl L. AU - Barnstead, Mary AU - Feldblyum, Tamara V. AU - Buell, C. Robin AU - Ketchum, Karen A. AU - Lee, John AU - Ronning, Catherine M. AU - Koo, Hean L. AU - Moffat, Kelly S. AU - Cronin, Lisa A. AU - Shen, Mian AU - Pai, Grace AU - Van Aken, Susan AU - Umayam, Lowell AU - Tallon, Luke J. AU - Gill, John E. AU - Adams, Mark D. AU - Carrera, Ana J. AU - Creasy, Todd H. AU - Goodman, Howard M. AU - Somerville, Chris R. AU - Copenhaver, Greg P. AU - Preuss, Daphne AU - Nierman, William C. AU - White, Owen AU - Eisen, Jonathan A. AU - Salzberg, Steven L. AU - Fraser, Claire M. AU - Venter, J. Craig PY - 1999 DA - 1999/12/01 TI - Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana JO - Nature SP - 761 EP - 768 VL - 402 IS - 6763 AB - Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130–140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2. SN - 1476-4687 UR - https://doi.org/10.1038/45471 DO - 10.1038/45471 ID - Lin1999 ER -