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TY - JOUR AU - Blake, William J. AU - KÆrn, Mads AU - Cantor, Charles R. AU - Collins, J. J. PY - 2003 DA - 2003/04/01 TI - Noise in eukaryotic gene expression JO - Nature SP - 633 EP - 637 VL - 422 IS - 6932 AB - Transcription in eukaryotic cells has been described as quantal1, with pulses of messenger RNA produced in a probabilistic manner2,3. This description reflects the inherently stochastic nature4,5,6,7,8,9 of gene expression, known to be a major factor in the heterogeneous response of individual cells within a clonal population to an inducing stimulus10,11,12,13,14,15,16. Here we show in Saccharomyces cerevisiae that stochasticity (noise) arising from transcription contributes significantly to the level of heterogeneity within a eukaryotic clonal population, in contrast to observations in prokaryotes15, and that such noise can be modulated at the translational level. We use a stochastic model of transcription initiation specific to eukaryotes to show that pulsatile mRNA production, through reinitiation, is crucial for the dependence of noise on transcriptional efficiency, highlighting a key difference between eukaryotic and prokaryotic sources of noise. Furthermore, we explore the propagation of noise in a gene cascade network and demonstrate experimentally that increased noise in the transcription of a regulatory protein leads to increased cell–cell variability in the target gene output, resulting in prolonged bistable expression states. This result has implications for the role of noise in phenotypic variation and cellular differentiation. SN - 1476-4687 UR - https://doi.org/10.1038/nature01546 DO - 10.1038/nature01546 ID - Blake2003 ER -