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TY - JOUR AU - Woltjen, Knut AU - Michael, Iacovos P. AU - Mohseni, Paria AU - Desai, Ridham AU - Mileikovsky, Maria AU - Hämäläinen, Riikka AU - Cowling, Rebecca AU - Wang, Wei AU - Liu, Pentao AU - Gertsenstein, Marina AU - Kaji, Keisuke AU - Sung, Hoon-Ki AU - Nagy, Andras PY - 2009 DA - 2009/04/01 TI - piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells JO - Nature SP - 766 EP - 770 VL - 458 IS - 7239 AB - The discovery that non-germline adult cells can be reprogrammed to become pluripotent, able to differentiate into any cell type, opened up exciting possibilities. Reprogrammed cells — called induced pluripotent stem (iPS) cells — should have great potential in regenerative medicine, but most current methods of producing them involve viral gene delivery that could cause abnormalities in the induced cells. Two groups in this issue report on a collaboration that has succeeded in producing pluripotency in human cells without using viral vectors. Stable iPS cells were produced in both human and mouse fibroblasts using virus-derived 2A peptide sequences to create a multicistronic vector incorporating the reprogramming factors, delivered to the cell by the piggyBac transposon vector. The 2A-linked reprogramming factors, not required in the established iPS cell lines, were then removed. SN - 1476-4687 UR - https://doi.org/10.1038/nature07863 DO - 10.1038/nature07863 ID - Woltjen2009 ER -