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TY - JOUR AU - Navin, Nicholas AU - Kendall, Jude AU - Troge, Jennifer AU - Andrews, Peter AU - Rodgers, Linda AU - McIndoo, Jeanne AU - Cook, Kerry AU - Stepansky, Asya AU - Levy, Dan AU - Esposito, Diane AU - Muthuswamy, Lakshmi AU - Krasnitz, Alex AU - McCombie, W. Richard AU - Hicks, James AU - Wigler, Michael PY - 2011 DA - 2011/04/01 TI - Tumour evolution inferred by single-cell sequencing JO - Nature SP - 90 EP - 94 VL - 472 IS - 7341 AB - Tumours are known to be genetically heterogeneous, but it is proving difficult to dissect this heterogeneity at the single-cell level. A combination of whole-genome amplification and sequencing of single nuclei separated by fluorescence activated cell sorting now reveals the population structure of breast tumours from two patients. In both, tumour growth is by punctuated clonal expansions with few persistent intermediates, in contrast to the many gradual models of tumour progression. Single-cell sequencing of this type — once it becomes cheaper — is likely to have clinical implications for cancer prognosis and staging. SN - 1476-4687 UR - https://doi.org/10.1038/nature09807 DO - 10.1038/nature09807 ID - Navin2011 ER -