(original) (raw)

TY - JOUR AU - Mahajan, Nupam P. AU - Linder, Katrina AU - Berry, Gail AU - Gordon, Gerald W. AU - Heim, Roger AU - Herman, Brian PY - 1998 DA - 1998/06/01 TI - Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer JO - Nature Biotechnology SP - 547 EP - 552 VL - 16 IS - 6 AB - It has been hypothesized that interaction of Bcl-2 and Bax may regulate apoptosis. The spatial and temporal interaction of Bcl-2 and Bax at the single cell level has not, however, been demonstrated. To achieve this goal, we have developed two-fusion FRET (fluorescence resonance energy transfer). Using green fluorescent protein (GFP)-Bax and blue fluorescent protein (BFP)-Bcl-2 fusion proteins coexpressed in the same cell, we demonstrate a direct interaction between Bcl-2 and Bax in individual mitochondria. Mitochondrially localized cytochrome c-GFP and BFP-Bcl-2 showed little or no FRET, while nuclear-localized GFP-human papillomavirus E6 and BFP-Bcl-2 did not interact when coexpressed in the same cell. These findings indicate that two-fusion FRET provides an opportunity to examine the interaction between two different proteins coexpressed in single intact mammalian cells. SN - 1546-1696 UR - https://doi.org/10.1038/nbt0698-547 DO - 10.1038/nbt0698-547 ID - Mahajan1998 ER -